Signal peptide-dependent protein transport in Bacillus subtilis: A genome-based survey of the secretome

Microbiology and Molecular Biology Reviews

Volumn 64, Issue 3, 2000, Pages 515-547

  Tjalsma, H ^a   Bolhuis, A ^a   Jongbloed, J D H ^a   Bron, S ^a   Van Dijl, J M ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; BACTERIAL PROTEIN; CARRIER PROTEIN; CHAPERONE; LIPOPROTEIN;

AMINO TERMINAL SEQUENCE; BACILLUS SUBTILIS; GENOME; MOLECULAR DYNAMICS; NONHUMAN; PROTEIN SECRETION; PROTEIN TRANSPORT; REVIEW; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION;

BACILLI; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; POSIBACTERIA; PROKARYOTA; SACCHAROMYCES; SACCHAROMYCES CERVISIAE;

EID: 0033818171     PISSN: 10922172     EISSN: None     Source Type: Journal    
DOI: 10.1128/MMBR.64.3.515-547.2000     Document Type: Review

References (341)

1. SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli
2. FtsH (HflB) is an ATP-dependent protease selectively acting on SecY and some other membrane proteins
3. The Saccharomyces cerevisiae OXA1 gene is required for the correct assembly of cytochrome c oxidase and oligomycin-sensitive ATP synthase
4. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
5. A new Escherichia coli gene, dsbG, encodes a periplasmic protein involved in disulfide bond formation, required for recycling DsbA/DsbB and DsbC redox proteins
6. Isolation and characterization of a sporulation initiation mutation in the Bacillus subtilis secA gene
7. Characterization of the pcp gene encoding the pyrrolidone carboxyl peptidase of Bacillus subtilis
8. Target cell specificity of a bacteriocin molecule: A C-terminal signal directs lysostaphin to the cell wall of Staphylococcus aureus
9. Targeting of muralytic enzymes to the cell division site of Gram-positive bacteria: Repeat domains direct autolysin to the equatorial surface ring of Staphylococcus aureus
10. Purification and biochemical analysis of WprA, a 52-kDa serine protease secreted by Bacillus subtilis as an active complex with its 23-kDa propeptide
11. Structure and expression of a gene encoding the precursor of subtilin, a small protein antibiotic
12. Synthesis of precursor maltose-binding protein with proline in the +1 position of the cleavage site interferes with the activity of Escherichia coli signal peptidase I in vivo
13. PET1402, a nuclear gene required for proteolytic processing of cytochrome oxidase subunit 2 in yeast
14. Specificity and control of uptake of purines and other compounds in Bacillus subtilis
15. Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor
16. How chaperones fold proteins
17. Subunit II of Bacillus subtilis cytochrome c oxidase is a lipoprotein
18. A common export pathway for proteins binding complex redox cofactors?
19. Role of Cue1p in ubiquitination and degradation at the ER surface
20. The role of autolysins during vegetative growth of Bacillus subtilis 168
21. Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein
22. Targeting of GroEL to SecA on the cytoplasmic membrane of Escherichia coli
23. Pathway specificity for a delta pH-dependent precursor thylakoid lumen protein is governed by a 'Sec-avoidance' motif in the transfer peptide and a 'Sec-incompatible' mature protein
24. An essential component of a novel bacterial protein export system with homologues in plastids and mitochondria
25. Sec11 is required for signal peptide processing and yeast cell growth
26. Bacillus subtilis can modulate its capacity and specificity for protein secretion by temporally controlled expression of the sips gene for signal peptidase I
27. SecDF of Bacillus subtilis, a molecular Siamese twin required for the efficient secretion of proteins
28. Different mechanisms for thermal inactivation of Bacillus subtilis signal peptidase mutants
29. Evaluation of bottlenecks in the late stages of protein secretion in Bacillus subtilis
30. Signal peptide peptidase- and ClpP-like proteins of Bacillus subtilis are required for efficient translocation and processing of secretory proteins
31. Functional analysis of paralogous thiol-disulfide oxidoreductases in Bacillus subtilis
32. Did the mitochondrial processing peptidase evolve from a eubacterial regulator of gene expression?
33. Cloning of a human gene involved in cytochrome oxidase assembly by functional complementation of an oxa1-mutation in Saccharomyces cerevisiae
34. Helix packing in membrane proteins
35. How many membrane proteins are there?
36. Improving protein secretion by engineering components of the bacterial translocation machinery
37. Conformations of signal peptides induced by lipids suggest initial steps in protein export
38. Targeting of thylakoid proteins by the delta pH-driven twin-arginine translocation pathway requires a specific signal in the hydrophobic domain in conjunction with the twin-arginine motif
39. Unusual characteristics of amino-terminal and hydrophobic domains in nuclear-encoded thylakoid signal peptides
40. Protein secretion and possible roles for multiple signal peptidases for precursor processing in bacilli
41. Molecular biology and genetics of Bacillus spp.
42. The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation
43. Isolation and sequence analysis of dacB, which encodes a sporulation-specific penicillin-binding protein in Bacillus subtilis
44. Complete genome sequence of the methanogenic archaeon Methanococcus jannaschii
45. RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators
46. A predictor of transmembrane alpha-helix domains of proteins based on neural networks
47. A new type of signal peptide: Central role of a twin-arginine motif in transfer signals for the delta pH-dependent thylakoidal protein translocase
48. Role of the leader and structural regions of prelantibiotic peptides as assessed by expressing nisin-subtilin chimeras in Bacillus subtilis 168, and characterization of their physical, chemical, and antimicrobial properties
49. Secretion of Bacillus subtilis levansucrase. Fe(III) could act as a cofactor in an efficient coupling of the folding and translocation processes
50. Anionic polymers of Bacillus subtilis cell wall modulate the folding rate of secreted proteins
51. Effect of alteration of charged residues at the N termini of signal peptides on protein export in Bacillus subtilis
52. The subtilin gene of Bacillus subtilis ATCC 6633 is encoded in an operon that contains a homolog of the hemolysin B transport protein
53. ComC is required for the processing and translocation of ComGC, a pilin-like competence protein of Bacillus subtilis
54. All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis
55. Cell surface localization and processing of the ComG proteins, required for DNA binding during transformation of Bacillus subtilis
56. A folded protein can be transported across the chloroplast envelope and thylakoid membranes
57. Escherichia coli prlC encodes an endopeptidase and is homologous to the Salmonella typhimurium opdA gene
58. A monomeric, tightly folded stromal intermediate on the ΔpH-dependent thylakoidal protein transport pathway
59. The signal recognition particle-targeting pathway does not necessarily deliver proteins to the Sec-translocase in Escherichia coli
60. Competition between Sec- and Tat-dependent protein translocation in Escherichia coli
61. Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: The dense alignment surface method
62. Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli
63. Forespore-specific transcription of a gene in the signal transduction pathway that governs pro-sigma K processing in Bacillus subtilis
64. Signal peptidases in prokaryotes and eukaryotes: A new protease family
65. Protein translocation into and across the bacterial plasma membrane and the plant thylaloid membrane
66. Leader peptidase catalyzes the release of exported proteins from the outher surface of the Escherichia coli plasma membrane
67. The chemistry and enzymology of the type I signal peptidases
68. A new heat-shock gene, ppiD, encodes a peptidyl-prolyl isomerase required for folding of outer membrane proteins in Escherichia coli
69. Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli
70. Domain interactions of the peripheral pre-protein translocase subunit SecA
71. The fish gene of Bacillus subtilis is involved in major cellular processes such as sporulation, stress adaptation and secretion
72. The ftsH gene of Bacillus subtilis is transiently induced after osmotic and temperature upshift
73. Maturation pathway of nisin and other lantibiotics: Post-translationally modified antimicrobial peptides exported by Gram-positive bacteria
74. Lipid involvement in protein translocation in Escherichia coli
75. Proteolytic activities in Bacillus
76. All cyclophilins and FK506 binding proteins are, individually and collectively, dispensable for viability in Saccharomyces cerevisiae
77. The Sec system
78. Bacterial protein translocation: Kinetic and thermodynamic role of ATP and the protonmotive force
79. How proteins cross the bacterial cytoplasmic membrane
80. Binding and transport of transforming DNA by Bacillus subtilis: The role of type-IV pilin-like proteins - A review
81. The mycosubtilin synthetase of Bacillus subtills ATCC 6633: A multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase
82. Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of pre-protein translocase holoenzyme
83. Biogenesis of the Gram-negative bacterial envelope
84. Bacterial pre-protein translocase: Mechanism and conformational dynamics of a processive enzyme
85. SecA promotes pre-protein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion
86. SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF
87. The protease-protected 30 kDa domain of SecA is largely inaccessible to the membrane lipid phase
88. Both an N-terminal 65-kDa domain and a C-terminal 30-kDa domain of SecA cycle into the membrane at SecYEG during translocation
89. Bacillus subtilis sporulation: Regulation of gene expression and control of morphogenesis
90. In vivo observation of polypeptide flux through the bacterial chaperonin system
91. Expression of the staphylococcal protein A gene in Bacillus subtilis by gene fusions utilizing the promoter from a Bacillus amyloliquefaciens α-amylase gene
92. The molecular chaperone SecB is released from the carboxyl terminus of SecA during initiation of precursor potein translocation
93. Pre-protein transfer to the Escherichia coli translocase requires the co-operative binding of SecB and the signal sequence to SecA
94. Protein targeting to the bacterial cytoplasmic membrane
95. Commercial production of extracellular enzymes
96. Analysis of the autolysins of Bacillus subtilis 168 during vegetative growth and differentiation by using renaturing polyacrylamide gel electrophoresis
97. Molecular analysis of three major wall-associated proteins of Bacillus subtilis 168: Evidence for processing of the product of a gene encoding a 258 kDa precursor two-domain ligand-binding protein
98. Membrane topology of the lactococcal bacteriocin ATP-binding cassette transporter protein LcnC. Involvement of LcnC in lactococcin a maturation
99. Characterization of the surfactin synthetase multi-enzyme complex
100. Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles
101. Signal peptide mutants of Escherichia coli
102. Binding site-shaped repeated sequences of bacterial wall peptidoglycan hydrolases
103. Prolipoprotein modification and processing in Escherichia coli: A unique secondary structure in prolipoprotein signal sequence for the recognition by glyceryl transferase
104. Versatile properties of a nonsaturable, homogeneous transport system in Bacillus subtilis: Genetic, kinetic, and affinity labeling studies
105. The yeast genome directory
106. Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane
107. Cyclophilin and trigger factor from Bacillus subtilis catalyze in vitro protein folding and are necessary for viability under starvation conditions
108. A family of cold shock proteins in Bacillus subtilis is essential for cellular growth and for efficient protein synthesis at optimal and low temperatures
109. Trigger factor depletion or overproduction causes defective cell division but does not block protein export
110. The binding cascade of SecB to SecA to SecY/E mediates pre-protein targeting to the E. coli plasma membrane
111. Highlight: Protein folding in vivo
112. A family of bacteriocin ABC transporters carry out proteolytic processing of their substrates concomitant with export
113. Heat-shock and general stress response in Bacillus subtilis
114. Oxa1p mediates the export of the N- and C-termini of pCoxII from the mitochondrial matrix to the intermembrane space
115. Oxa1p, an essential component of the N-tail protein export machinery, in mitochondria
116. Temporal expression of the Bacillus subtilis secA gene, encoding a central component of the preprotein translocase
117. Escherichia coli trigger factor is a prolyl isomerase that associates with nascent polypeptide chains
118. Proteasomes: Destruction as a programme
119. Proteome analysis of Bacillus subtilis extracellular proteins: A two-dimensional protein electrophoretic study
120. Activation of the proprotein transcription factor prosigma E is associated with its progression through three patterns of subcellular localization during sporulation in Bacillus subtilis
121. Extracellular signal protein triggering the proteolytic activation of a developmental transcription factor in Bacillus subtilis
122. Cloning and characterization of a Bacillus subtilis gene encoding a homolog of the 54-kilodalton subunit of mammalian signal recognition particle and Escherichia coli Ffh
123. Signal peptide digestion in Escherichia coli: Effect of protease inhibitors on hydrolysis of the cleaved signal peptide of the major outer-membrane lipoprotein
124. The sec-independent twin-arginine translocation system can transport both tightly folded and malfolded proteins across the thylakoid membrane
125. Protease IV, a cytoplasmic membrane protein of Escherichia coli, has signal peptide peptidase activity
126. Molecular cloning and sequencing of the sppA gene and characterization of the encoded protease IV, a signal peptide peptidase
127. In vitro processing of pro-subtilisin produced in Escherichia coli
128. Requirement of pro-sequence for the production of active subtilsin E in Escherichia coli
129. Cloning and characterization of the gene for a protein thioldisulfide oxidoreductase in Bacillus brevis
130. Bacillus subtilis PrsA is required in vivo as an extracytoplasmic chaperone for secretion of active enzymes synthesized either with or without pro-sequences
131. Isolation and characterization of the secE homologue gene of Bacillus subtilis
132. Differential processing of propeptide inhibitors of Rap phosphatases in Bacillus subtilis
133. Protein folding activities of Escherichia coli protein disulfide isomerase
134. Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases
135. Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA
136. Dislocation of membrane proteins in FtsH-mediated proteolysis
137. SecA protein is exposed to the periplasmic surface of the Escherichia coli inner membrane in its active state
138. Genes involved in self-protection against the lantibiotic subtilin produced by Bacillus subtilis ATCC 6633
139. The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus
140. Lysine 106 of the putative catalytic ATP-binding site of the Bacillus subtilis SecA protein is required for functional complementation of Escherichia coli secA mutants in vivo
141. Substrate specificity of the SecB chaperone
142. In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli
143. Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis
144. The PrsA lipoprotein is essential for protein secretion in Bacillus subtilis and sets a limit for high-level secretion
145. A gene (prsA) of Bacillus subtilis involved in a novel, late stage of protein export
146. ER quality control: The cytoplasmic connection
147. Processing of lipid-modified prolipoprotein requires energy and sec gene products in vivo
148. Highly selective binding of nascent polypeptides by an Escherichia coli chaperone protein in vivo
149. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
150. Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli
151. The type IV prepilin peptidases comprise a novel family of aspartic acid proteases
152. An exported peptide functions intracellularly to contribute to cell density signaling in Bacillus subtilis
153. Lipid modification of prelipoproteins is dispensable for growth but essential for efficient protein secretion in Bacillus subtilis: Characterization of the lgt gene
154. The 'trigger factor cycle' includes ribosomes, presecretory proteins and the plasma membrane
155. SecA protein hydrolyses ATP and is an essential component of the protein translocation ATPase of Escherichia coli
156. The ATPase activity of SecA is regulated by acidic phospholipids, SecY, and the leader and mature domains of precursor proteins
157. Leader peptidase of type IV prepilins related proteins
158. A role for trigger factor and an Rgg-like regulator in the transcription, secretion and processing of the cysteine proteinase of Streptococcus pyogenes
159. Biochemical and genetic characterization of a competence pheromone from Bacillus subtilis
160. Extreme stabilization of a thermolysin-like protease by an engineered disulfide bond
161. In vivo cross-linking of the SecA and SecY subunits of the Escherichia coli preprotein translocase
162. A new Bacillus subtilis gene with homology to Escherichia coli prc
163. The wprA gene of Bacillus subtilis 168, expressed during exponential growth, encodes a cell-wall-associated protease
164. The lytE gene of Bacillus subtilis 168 encodes a cell wall hydrolase
165. Roles of SecG in ATP- and SecA-dependent protein translocation
166. SecD is involved in the release of translocated secretory proteins from the cytoplasmic membrane of Escherichia coli
167. A novel periplasmic carrier protein involved in the sorting and transport of Escherichia coli lipoproteins destined for the outer membrane
168. A novel outer membrane lipoprotein, Lo1B (HemM), involved in the Lo1A (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli
169. Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
170. Use of the pre-pro part of Staphylococcus hyicus lipase as a carrier for secretion of Escherichia coli outer membrane protein A (OmpA) prevents proteolytic degradation of OmpA by cell-associated protease(s) in two different gram-positive bacteria
171. The endogenous Bacillus subtilis (natto) plasmids pTA1015 and pTA1040 contain signal peptidase-encoding genes: Identification of a new structural module on cryptic plasmids
172. Rolling-circle plasmids from Bacillus subtilis: Complete nucleotide sequences and analyses of genes of pTA1015, pTA1040, pTA1050 and pTA1060, and comparisons with related plasmids from Gram-positive bacteria
173. A periplasm in Bacillus subtilis
174. Protein misfolding in the cell envelope of Escherichia coli: New signaling pathways
175. Expression of a germination-specific amidase, SleB, of bacilli in the forespore compartment of sporulating cells and its localization on the exterior side of the cortex in dormant spores
176. ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation
177. Two-component regulatory systems
178. Suppression of the growth and export defects of an Escherichia coli secA(Ts) mutant by a gene cloned from Bacillus subtilis
179. Chaperone-like activities of the CsaA protein of Bacillus subtilis
180. Interaction of Bacillus subtilis CsaA with SecA and precursor proteins
181. A mutation affecting signal peptidase inhibits degradation of an abnormal membrane protein in Saccharomyces cerevisiae
182. Identification and characterization of pbpC, the gene encoding Bacillus subtilis penicillin-binding protein 3
183. Identification and characterization of pbpA, encoding Bacillus subtilis penicillin-binding protein 2A
184. Membrane topology of Escherichia coli prolipoprotein signal peptidase (signal peptidase II)
185. Protein secretion
186. Cloning and characterization of a Bacillus subtilis gene homologous to E. coli secY
187. Small cytoplasmic RNA of Bacillus subtilis: Functional relationship with human signal recognition particle 7S RNA and Escherichia coli 4.5S RNA
188. The Bacillus subtilis Srp54 homologue, Ffh, has an intrinsic Gtpase activity and forms a ribonucleoprotein complex with small cytoplasmic Rna in vivo
189. Bacillus subtilis histone-like protein, HBsu, is an integral component of a SRP-like particle that can bind the Alu domain of small cytoplasmic RNA
190. Molecular biology of antibiotic production in Bacillus
191. Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in Gram-positive bacteria
192. Cell wall sorting of lipoproteins in Staphylococcus aureus
193. Novel lantibiotics and their pre-peptides
194. Protein folding in the cytosol: Chaperonin-dependent and -independent mechanisms
195. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites
196. Import of barley photosystem I subunit N into the thylakoid lumen is mediated by a bipartite presequence lacking an intermediate processing site: Role of the delta pH in translocation across the thylakoid membrane
197. A signal peptide with a proline next to the cleavage site inhibits leader peptidase when present in a Sec-independent protein
198. Localization and purification of two enzymes from Escherichia coli capable of hydrolyzing a signal peptide
199. Degradation of a signal peptide by protease IV and oligopeptidase A
200. Product of the Pseudomonas aeruginosa gene pilD is a prepilin leader peptidase
201. srb: A Bacillus subtilis gene encoding a homologue of the alpha-subunit of the mammalian signal recognition particle receptor
202. Identification of a gene fragment which codes for the 364 amino-terminal amino acid residues of a SecA homologue from Bacillus subtilis: Further evidence for the conservation of the protein export apparatus in Gram-positive and Gram-negative bacteria
203. Crystal structure of a bacterial signal peptidase in complex with a beta-lactam inhibitor
204. Identification and characterization of the structural and transporter genes for, and the chemical and biological properties of, sublancin 168, a novel lantibiotic produced by Bacillus subtilis 168
205. Molecular cloning of α-amylase gene from Bacillus amyloliquefaciens and its expression in Bacillus subtilis
206. Engineering for secretion of proteins by bacteria
207. Multiple protein-aspartate phosphatases provide a mechanism for the integration of diverse signals in the control of development in Bacillus subtilis
208. Aspartyl-phosphate phosphatases deactivate the response regulator components of the sporulation signal transduction system in Bacillus subtilis
209. Cell-cell communication regulates the effects of protein aspartate phosphatases on the phosphorelay controlling development in Bacillus subtilis
210. A peptide export-import control circuit modulating bacterial development regulates protein phosphatases of the phosphorelay
211. The biosynthesis of rat serum albumin: In vivo studies on the formation of the disulfide bonds
212. The contribution of the cell wail to a transmembrane calcium gradient could play a key role in Bacillus subtilis protein secretion
213. Bacillus subtilis levansucrase: Amino acid substitutions at one site affect secretion efficiency and refolding kinetics mediated by metals
214. Signal sequence recognition in posttranslational protein transport across the yeast ER membrane
215. Mutant analysis links the translocon and BiP to retrograde protein transport for ER degradation
216. The Cs sec mutants of Escherichia coli reflect the cold sensitivity of protein export itself
217. Protein translocation in the three domains of life: Variations on a theme
218. Overall protein content and induced enzyme components of the periplasm of Bacillus subtilis
219. The Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is part of a three-gene operon required for proper spore cortex synthesis and spore core dehydration
220. Analysis of the peptidoglycan structure of Bacillus subtilis endospores
221. Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis
222. The role of the lipoprotein sorting signal (aspartate +2) in pullulanase secretion
223. Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor
224. The signal peptidase II (lsp) gene of Bacillus subtilis
225. Separable ATPases and membrane insertion domains of the secA subunit of pre-protein translocase
226. The complete general secrotory pathway in gram-negative bacteria
227. The general protein-export pathway is directly required for extracellular pullulanase secretion in Escherichia coli K12
228. Merits of secretion of heterologous proteins from industrial micro-organisms
229. Confirmation of the existence of a third family among peptidyl-prolyl cis/trans isomerases: Amino acid sequence and recombinant production of parvulin
230. Glucosaminidase of Bacillus subtilis: Cloning, regulation, primary structure and biochemical characterization
231. Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene
232. The ins and outs of protein translocation
233. The presequence of a chimeric construct dictates which of two mechanisms are utilized for translocation across the thylakoid membrane: Evidence for the existence of two distinct translocation systems
234. Targeting of proteins into and across the thylakoid membrane
235. Contribution of novel choline-binding proteins to adherence, colonization and immunogenicity of Streptococcus pneumoniae
236. Membrane topology of the 60-kDa Oxa1p homologue from Escherichia coli
237. Effect of septum initiation mutation on sporulation and competent cell formation in Bacillus subtilis
238. Bacillus subtilis gene involved in cell division, sporulation, and exoenzyme scretion
239. + gene of Bacillus subtilis to the Escherichia coli secA gene
240. Biosynthesis and biological activities of lantibiotics with unique post-translational modifications
241. Searching for autolysin functions: Characterization of a pneumococcal mutant deleted in the lytA gene
242. Cloning and expression of gene fragments encoding the choline-binding domain of pneumococcal murein hydrolases
243. Lipid modification of bacterial prolipoprotein; transfer of diacylglyceryl moiety from phosphatidylglycerol
244. Modification of bacterial lipoproteins
245. Roles of histidine-103 and tyrosine-235 in the function of the prolipoprotein diacylglyceryl transferase of Escherichia coli
246. A novel Sec-independent periplasmic protein translocation pathway in Escherichia coli
247. Overlapping functions of components of a bacterial Sec-independent protein export pathway
248. Sec-independent protein translocation in Escherichia coli: A distinct and pivotal role for the TatB protein
249. Secretion of human serum albumin from Bacillus subtilis
250. Common principles of protein translocation across membranes
251. The secE gene encodes an integral membrane protein required for protein export in Escherichia coli
252. Δμ(H)+ and ATP function at different steps of the catalytic cycle of pre-protein translocase
253. Structure of the cell wall anchor of surface proteins in Staphylococcus aureus
254. Cell wall sorting signals in surface proteins of Gram-positive bacteria
255. Sorting of protein A to the staphylococcal cell wall
256. Expression of a pepT homologue from Bacillus subtilis
257. SecA is not required for signal recognition particle-mediated targeting and initial membrane insertion of a nascent inner membrane protein
258. YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase
259. FtsY, the prokaryotic signal recognition particle receptor homologue, is essential for biogenesis of membrane proteins
260. A Bacillus subtilis secreted protein with a role in endospore coat assembly and function
261. Sec-independent protein translocation by the maize Hcf106 protein
262. Testing the '+2 rule' for lipoprotein sorting in the Escherichia coli cell envelope with a new genetic selection
263. The proton motive force lowers the level of ATP required for the in vitro translocation of a secretory protein in Escherichia coli
264. Each of the activities of signal recognition particle (SRP) is contained within a distinct domain: Analysis of biochemical mutants of SRP
265. Protein secretion in Bacillus species
266. Predicting the topology of eukaryotic membrane proteins
267. A gene required for nutritional repression of the Bacillus subtilis dipeptide permease operon
268. Gene encoding a minor extracellular protease in Bacillus subtilis
269. Bacillopeptidase F of Bacillus subtilis: Purification of the protein and cloning of the gene
270. Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis
271. Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: Functional analysis and comparative genomics
272. Identification of a region of interaction between Escherichia coli SecA and SecY proteins
273. Convergent sensing pathways mediate response to two extracellular competence factors in Bacillus subtilis
274. Purification and characterization of an extracellular peptide factor that affects two different developmental pathways in Bacillus subtilis
275. Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation
276. Influence of a cell wall-associated protease on production of alpha-amylase by Bacillus subtilis
277. The influence of protein folding on late stages of the secretion of α-amylases from Bacillus subtilis
278. Secretion, localization, and antibacterial activity of TasA, a Bacillus subtilis spore-associated protein
279. Regulation of synthesis of the Bacillus subtilis transition-phase, spore-associated antibacterial protein TasA
280. Control of synthesis and secretion of the Bacillus subtilis protein YqxM
281. Molecular genetics of sporulation in Bacillus subtilis
282. Isolation of a secY homologue from Bacillus subtilis: Evidence for a common protein export pathway in eubacteria
283. Lipoproteins of gram-positive bacteria
284. Characterization of the sppA gene encoding protease IV, a signal peptide peptidase of Escherichia coli
285. Pre-protein translocation by a hybrid translocase composed of Escherichia coli and Bacillus subtilis subunits
286. In vivo and in vitro characterization of the secA gene product of Bacillus subtilis
287. 2-terminal processing of Bacillus subtilis α-amylase
288. 2-terminal proteolysis and COOH-terminal CAAX processing
289. Genetic analysis of SecY: Additional export-defective mutations and factors affecting their phenotypes
290. From one gene to two proteins: The biogenesis of cytochromes b and c1 in Bradyrhizobium japonicum
291. Bacillus subtilis contains four closely related type I signal peptidases with overlapping substrate specificities: Constitutive and temporally controlled expression of different sip genes
292. Functional analysis of the secretory precursor processing machinery of Bacillus subtilis: Identification of a eubacterial homolog of archaeal and eukaryotic signal peptidases
293. The role of lipoprotein processing by signal peptidase II in the Gram-positive eubacterium Bacillus subtilis: Signal peptidase II is required for the efficient secretion of α-amylase, a non-lipoprotein
294. The plasmid-encoded type I signal peptidase SipP can functionally replace the major signal peptidases Sips and SipT of Bacillus subtilis
295. The potential active site of the lipoprotein-specific (type II) signal peptidase of Bacillus subtilis
296. Conserved serine and histidine residues are critical for activity of the ER-type signal peptidase SipW of Bacillus subtilis
297. Post-translational modification and processing of Escherichia coli prolipoprotein in vitro
298. Anchor structure of staphylococcal surface proteins. A branched peptide that links the carboxyl terminus of proteins to the cell wall
299. Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif
300. Cloning and expression of a novel protease gene encoding an extracellular neutral protease from Bacillus subtilis
301. A serine and a lysine residue implicated in the catalytic mechanism of the Escherichia coli leader peptidase
302. The Escherichia coli signal recognition particle is required for the insertion of a subset of inner membrane proteins
303. Early events in pre-protein recognition in E. coli: Interaction of SRP and trigger factor with nascent polypeptides
304. Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor
305. The Escherichia coli SRP and SecB targeting pathways converge at the translocon
306. Interaction between SecA and SecYEG in micellar solution and formation of the membrane-inserted state
307. The catalytic cycle of the Escherichia coli SecA ATPase comprises two distinct pre-protein translocation events
308. Identification of the potential active site of the signal peptidase Sips of Bacillus subtilis: Structural and functional similarities with LexA-like proteases
309. Synthesis and processing of Escherichia coli TEM-β-lactamase and Bacillus licheniformis α-amylase in Escherichia coli: The role of signal peptidase I
310. Signal peptidase I of Bacillus subtilis: Patterns of conserved amino acids in prokaryotic and eukaryotic type I signal peptidases
311. The signal peptidase SipS
312. In vitro membrane integration of leader peptidase depends on the Sec machinery and anionic phospholipids and can occur post-translationally
313. Production of human interleukin-3 using industrial micro-organisms
314. The catalytic mechanism of endoplasmic reticulum signal peptidase appears to be distinct from most eubacterial signal peptidases
315. Functional identification of the product of the Bacillus subtilis yvaL gene as a SecG homologue
316. Analysis of a calcium binding surface loop critical for the stability of the thermolysin-like protease of Bacillus stearothermophilus
317. The genes for secretion and maturation of lactococcins are located on the chromosome of Lactococcus lactis IL1403
318. Analysis of the induction of general stress proteins of Bacillus subtilis
319. How signal sequences maintain cleavage specific
320. The structure of signal peptides from bacterial lipoproteins
321. Protein targeting signals
322. The signal peptide
323. Life and death of a signal peptide
324. Species-specific variation in signal peptide design. Implications for protein secretion in foreign hosts
325. Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms
326. Secretion, processing and activation of bacterial extracellular proteases
327. Sequence of active site peptides from the penicillin-sensitive D-alanine carboxypeptidase of Bacillus subtilis: Mechanism of penicillin action and sequence homology to beta-lactamases
328. A novel and ubiquitous system for membrane targeting and secretion of cofactor-containing proteins
329. Sec61-mediated transfer of a membrane protein from the endoplasmic reticulum to the proteasome for destruction
330. DnaK and DnaJ heat shock proteins participate in protein export in Escherichia coli
331. Involvement of the DnaK-DnaJ-GrpE chaperone team in protein secretion in Escherichia coli
332. Advances in the use of Bacillus subtilis for the expression and secretion of heterologous proteins
333. Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase
334. Enhanced secretory production of a single-chain antibody fragment from Bacillus subtilis by coproduction of molecular chaperones
335. Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases
336. Depletion of Bacillus subtilis histone-like protein, HBsu, causes defective protein translocation and induces upregulation of small cytoplasmic RNA
337. Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation
338. Cloning and sequencing of the cell division gene pbpB, which encodes penicillin-binding protein 2B in Bacillus subtilis
339. Genes of the sbo-alb locus of Bacillus subtilis are required for production of the antilisterial bacteriocin subtilosin
340. Pro-sequence of subtilisin can guide the refolding of denatured subtilisin in an intermolecular process
341. The role of molecular chaperones in protein transport into mammalian endoplasmic reticulum