Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its application to enantioselective resolution in organic solvent

Biotechnology and Bioengineering

Volumn 90, Issue 2, 2005, Pages 223-230

  Lee, Seung Hwan ^a   Choi, Jong Il ^a   Han, Mee Jung ^a   Choi, Jong Hyun ^a   Lee, Sang Yup ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Cell surface display; Enantioselective resolution; Lipase; OprF; Recombinant E. coli; Whole cell biocatalyst

Indexed keywords

BIOLOGICAL MEMBRANES; BIOTECHNOLOGY; CELLS; GENES; IMMUNOLOGY; ORGANIC SOLVENTS; PROTEINS;

IMMUNOFLUORESCENCE MICROSCOPY; LIPASE; PSEUDOMONAS FLUORESCENS; SURFACE DISPLAY SYSTEM;

ESCHERICHIA COLI;

1 PHENYLETHANOL; BACTERIAL PROTEIN; HEXANE; OPRF LIPASE FUSION PROTEIN; OPRF PROTEIN; ORGANIC SOLVENT; OUTER MEMBRANE PROTEIN; PHENYLETHYL ACETATE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

AQUEOUS SOLUTION; ARTICLE; BACTERIAL GENE; BIOCATALYST; BIOTECHNOLOGY; CARBOXY TERMINAL SEQUENCE; CELL SURFACE; CELL SURFACE DISPLAY; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME STABILITY; ESCHERICHIA COLI; GENE DELETION; GENE FUSION; IMMUNOFLUORESCENCE MICROSCOPY; INCUBATION TIME; NONHUMAN; OPRF GENE; PROTEIN LOCALIZATION; PROTEIN MOTIF; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS FLUORESCENS; RACEMIC MIXTURE; SIGNAL DETECTION; SIK W1 LIPASE GENE; WESTERN BLOTTING; WHOLE CELL BIOCATALYST;

BACTERIAL OUTER MEMBRANE PROTEINS; BENZYL ALCOHOLS; BLOTTING, WESTERN; DNA, BACTERIAL; ESCHERICHIA COLI; LIPASE; MICROSCOPY, FLUORESCENCE; POLYMERASE CHAIN REACTION; SOLVENTS; STEREOISOMERISM;

EID: 17644382948     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20399     Document Type: Article

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