Proteome analysis of Bacillus subtilis extracellular proteins: A two-dimensional protein electrophoretic study

Microbiology

Volumn 146, Issue 1, 2000, Pages 65-75

  Hirose, Isao ^a   Sano, Kazuyoshi ^a   Shioda, Izumi ^a   Kumano, Miyuki ^a   Nakamura, Kouji ^a   Yamane, Kunio ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Bacillus subtilis; Extracellular proteins; Proteome analysis; SecA and ffh mutants; Two dimensional gel electrophoresis

Indexed keywords

BACTERIAL PROTEIN; CELLOBIOSE; GLUCOSE; MALTOSE; PROTEOME; SIGNAL PEPTIDE; STARCH;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIUM CULTURE; COMPARATIVE STUDY; CONTROLLED STUDY; CULTURE MEDIUM; MEMBRANE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; TEMPERATURE SENSITIVE MUTANT; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); BACTERIUM SUBTILIS; POSIBACTERIA;

EID: 0033972848     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-146-1-65     Document Type: Article

References (52)

1. Antelmann, H., Bernhardt, J., Schmid, R., March, H., Volker, U. & Hecker, M (1997). First steps from two-dimensional protein index towards a response-regulation map for Bacillus subtilis. Electrophoresis 18, 1451-1463.
2. Beltzer, J. P., Wessels, H. P. & Spiess, M. (1989). Signal peptidase can cleave inside a polytopic membrane protein. FEBS Lett 253, 93-98.
3. Berks, B. C. (1996). A common export pathway for proteins binding complex redox cofactors? Mol Microbiol 22, 393-404.
4. Bol, D. K. & Yasbin, R. E. (1991). The isolation, cloning and identification of a vegetative catalase gene from Bacillus subtilis. Gene 109, 31-37.
5. Bolhuis, A., Broekhuizen, C. P., Sorokin, A., van Roosmalen, M. L., Venema, G., Bron, S., Quax, W. & van Dijl, J. M. (1998). SecDF of Bacillus subtilis, a molecular siamese twin required for the efficient secretion of proteins. J Biol Chem 273, 21217-21224.
6. Bunai, K., Takamatsu, H., Horinaka, T., Oguro, A., Nakamura, K. & Yamane, K. (1996). Bacillus subtilis Ffh, a homologue of mammalian SRP54, can intrinsically bind to the precursors of secretory proteins. Biochem Biophys Res Commun 227, 762-767.
7. Bunai, K., Yamada, K., Hayashi, K., Nakamura, K. & Yamane, K. (1999). Enhancing effect of Bacillus subtilis Ffh, a homologue of the SRP54 subunit of the mammalian signal recognition particle, on the binding of SecA to precursors of secretory proteins in vitro. J Biochem 125, 151-159.
8. Collier, D. N., Bankaitis, V. A., Weiss, J. B. & Bassford, P. J., Jr (1988). The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein. Cell 53, 273-283.
9. DeLange, R. J., Chang, J. Y., Shaper, J. H. & Glazer, A. N. (1976). Amino acid sequence of flagellin of Bacillus subtilis 168. III. Tryptic peptides, N-bromosuccinimide peptides, and the complete amino acid sequence. J Biol Chem 251, 705-711.
10. Douville, K., Price, A., Eichler, J., Economou, A. & Wickner, W. (1995). SecYEG and SecA are the stoichiometric components of preprotein translocase. J Biol Chem 270, 20106-20111.
11. Foster, S. J. (1993). Molecular analysis of the three major wall-associated proteins of Bacillus subtilis 168: evidence for processing of the product of a gene encoding a 258 kDa precursor two-domain ligand-binding protein. Mol Microbiol 8, 299-310.
12. Gosalbes, M. J., Perez-Gonzalez, J. A., Gonzalez, R. & Navarro, A. (1991). Two β-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression and sequence analysis of genes encoding a xylanase and an endo β-(1,3)-(1,4)-glucanase. J Bacteriol 173, 7705-7710.
13. Graumann, P., Schroder, K., Schmid, R. & Marohiel, M. A. (1996). Cold shock stress-induced proteins in Bacillus subtilis. J Bacteriol 178, 4611-4619.
14. Hartle, F.-U., Lecker, S., Schiebel, E., Hendrick, T. P. & Wickner, W. (1990). The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane. Cell 63, 269-279.
15. Henner, D. I. (1990). Inducible expression of regulatory genes in Bacillus subtilis. Methods Enzymol 185, 223-228.
16. Honda, K., Nakamura, K., Nishiguchi, M. & Yamane, K. (1993). Cloning and characterization of a Bacillus subtilis gene encoding a homologue of the 54 kilodalton subunit of mammalian signal recognition particle and Escherichia coli Ffh. J Bacteriol 175, 4885-4894.
17. Hueck, C. J. (1998). Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev 62, 379-433.
18. Izui, K., Nielsen, J. B. K., Caulfield, M. P. & Lampen, J. O. (1980). Large exopenicillinase, initial extracellular form detected in cultures of Bacillus licheniformis. Biochemistry 19, 1882-1886.
19. Keenan, R. J., Freymann, D. M., Walter, P. & Stroud, R. M. (1998). Crystal structure of the signal-sequence-binding subunit of the signal recognition particle. Cell 94, 181-191.
20. Kunst, F., Ogasawara, N., Moszer, I. & 148 other authors (1997). The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature 390, 249-256.
21. Lutcke, H. (1995). Signal recognition particle (SRP), a ubiquitous initiator of protein translocation. Eur J Biochem 228, 531-550.
22. Lutcke, H., High, S., Romisch, K., Ashford, A. J. & Dobberstein, B. (1992). The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences. EMBO J 11, 1543-1551.
23. Margot, P. & Karamata, D. (1996). The wprA gene of Bacillus subtilis 168, expressed during exponential growth, encodes a cell-wall-associated protease. Microbiology 142, 3437-3444.
24. Matsudaira, P. (1987). Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membrane. J Biol Chem 262, 10035-10038.
25. Miyakawa, Y. & Komano, T. (1980). Study of the cell cycle of Bacillus subtilis using temperature sensitive mutants. I. Isolation and genetic analysis of the mutants defective in septum formation. Mol Gen Genet 181, 207-214.
26. Morrissey, J. H. (1981). Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 117, 307-310.
27. Nakamura, K., Yahagi, S., Yamazaki, T. & Yamane, K. (1999). Bacillus subtilis histone-like protein, HBsu, is an integral component of an SRP-like particle that can bind the Alu domain of small cytoplasmic RNA. J Biol Chem 274, 13569-13576.
28. Namba, K., Yamashita, I. & Vonderviszt, F. (1989). Structure of the core and central channel of bacterial flagella. Nature 342, 648-654.
29. Nasser, W., Awade, A. C., Reverchon, S. & Robert-Baudony, J. (1993). Pectate lyase from Bacillus subtilis: molecular characterization of the gene, and properties of the cloned enzyme. FEBS Lett 335, 319-326.
30. Nishiyama, K., Suzuki, T. & Tokuda, H. (1996). Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation. Cell 85, 71-81.
31. Nohara, T., Nakai, M., Goto, A. & Endo, T. (1995). Isolation and chracterization of the cDNA for pea chloroplast SecA : evolutionary conservation of the bacterial-type SecA-dependent protein transport within chloroplasts. FEBS Lett 364, 305-308.
32. Rabilloud, T., Adessi, C., Giraudel, A. & Lunardi, J. (1997). Improvement of the solubilization of proteins in two-dimensional electroporesis with immobilized pH gradient. Electrophoresis 18, 307-311.
33. Rapoport, T. A., Jungnickel, B. & Kutay, U. (1996). Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membrane. Annu Rev Biochem 65, 271-303.
34. Sadaie, Y., Takamatsu, H., Nakamura, K. & Yamane, K. (1991). Sequencing reveals similarity of the wild type div + gene of Bacillus subtilis to the Escherichia coli secA gene. Gene 98, 101-105.
35. Sargent, F., Bogsch, E. G., Stanley, N. R., Wexler, M., Robinson, C., Berks, B. C. & Palmer, T. (1998). Overlapping functions of components of a bacterial Sec-independent protein export pathway. EMBO J 17, 3640-3650.
36. Sloma, A., Rufo, G. A., Theriault, K. A., Dwyer, M., Wilson, S. W. & Pero, J. (1991). Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis. J Bacteriol 173, 6889-6895.
37. Stephenson, K. & Harwood, C. R. (1998). Influence of a cell-wall-associated protease on production of α-amylase by Bacillus subtilis. Appl Environ Microbiol 64, 2875-2881.
38. Stöver, A. G. & Driks, A. (1999). Secretion, localization, and antibacterial activity of TasA, a Bacillus subtilis spore-associated protein. J Bacteriol 181, 1664-1672.
39. Takamatsu, H., Fuma, S., Nakamura, K., Sadaie, Y., Shinkai, A., Matsuyama, S., Mizushima, S. & Yamane, K. (1992). In vivo and in vitro characterization of the secA gene product of Bacillus subtilis. J Bacteriol 174, 4308-4316.
40. Takamatsu, H., Nakane, A., Sadaie, Y., Nakamura, K. & Yamane, K. (1994). The rapid degradation of mutant SecA protein in Bacillus subtilis secA341(ts) mutant causes a protein-translocation defect in the cell. Biosci Biotechnol Biochem 58, 1845-1850.
41. Takamatsu, H., Bunai, K., Horinaka, T., Oguro, A., Nakamura, K. & Yamane, K. (1997). Identification of a region required for binding to presecretory protein in Bacillus subtilis Ffh, a homoloque of 54 kDa subunit of mammalian signal recognition particle. Eur J Biochem 248, 575-582.
42. Tjalsma, H., Bolhuis, A., van Roosmalen, M. L. & 7 other authors (1998). Functional analysis of the secretory precursor processing machinery of Bacillus snbtilis: identification of a eubacterial homolog of archeal and eukaryotic signal peptidases. Genes Dev 12, 2318-2331.
43. Tjalsma, H., Kontinen, V. P., Pragai, Z., Wu, H., Meima, R., Venema, G., Bron, S., Sarvas, M. & van Dijl, J. M. (1999). The role of lipoprotein processing by signal peptidase II in the Gram-positive eubacterium Bacillus subtilis: signal peptidease II is required for the efficient secretion of α-amylase, a non-lipoprotein. J Biol Chem 274, 1698-1707.
44. Tominaga, Y. & Tsujisaka, Y. (1975). Purification and some enzymatic properties of the chitosanase from Bacillus R-4 which lyses Phizoprus cell walls. Biochim Biophys Acta 410, 145-155.
45. Ulbrandt, N. D., Newitt, J. A. & Bernstein, H. D. (1997). The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins. Cell 88, 187-196.
46. Valent, Q. A., Scotti, P. A., High, S., de Gier, J.-W. L., von Heijne, G., Lentzen, G., Wintermayer, W., Oudega, B. & Luirink, J. (1998). The Escherichia coli SRP and SecB targeting pathways converge at the translocon. EMBO J 17, 2504-2512.
47. Walter, P. & Blobel, G. (1982). Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum. Nature 299, 691-698.
48. Walter, P. & Johnson, A. E. (1994). Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane. Annu Rev Cell Biol 10, 87-119.
49. Weiner, J. H., Bilous, P. T., Shaw, G. M., Lubitz, S. P., Frost, L., Thomas, G. H., Cole, J. A. & Turner, R. J. (1998). A novel and ubiquitous system for membrane targeting and secretion of cofactor-containing proteins. Cell 93, 93-101.
50. van Wely, K. H. M., Swaving, J., Broekhuizen, C. P., Rose, M., Quax, W. J. & Driessen, A. J. M. (1999). Functional identification of the product of the Bacillus subtilis yvaI. gene as a SecG homologue. J Bacteriol 181, 1786-1792.
51. Yamazaki, H., Ohmura, K., Nakayama, A., Takeichi, Y., Otozai, K., Yamasaki, M., Tamura, G. & Yamane, K. (1983). α-Amylase genes (amyR2 and amyE +) from an α-amylase hyperproducing Bacillus subtilis strain: molecular cloning and nucleotide sequences. J Bacteriol 156, 327-337.
52. Yang, M., Galizzi, A. & Henner, D. J. (1983). Nucleotide sequence of the amylase gene from Bacillus subtilis. Nucleic Acids Res 11, 237-249.