Monitoring of genes that respond to overproduction of an insoluble recombinant protein in Escherichia coli glucose-limited fed-batch fermentations

Biotechnology and Bioengineering

Volumn 70, Issue 2, 2000, Pages 217-224

  Jürgen, Britta ^a   Lin, Hong Ying ^b   Riemschneider, Stefan ^b   Scharf, Christian ^a   Neubauer, Peter ^b   Schmid, Roland ^c   Hecker, Michael ^a   Schweder, Thomas ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^c UNIVERSITY OF OSNABRÜCK   (Germany)

Author keywords

Escherichia coli; Fed batch; Inclusion bodies; Overexpression; Stress mRNAs; Stress proteins

Indexed keywords

AGGLOMERATION; BATCH CELL CULTURE; COMPOSITION EFFECTS; ESCHERICHIA COLI; FERMENTATION; GENES; MASS SPECTROMETRY; RNA; SOLUBILITY; YEAST;

ALPHA GLUCOSIDASE; GENE CODING;

ENZYMES;

ALPHA GLUCOSIDASE; BACTERIAL PROTEIN; BACTERIAL RNA; CHAPERONE; GLUCOSE; HEAT SHOCK PROTEIN; MESSENGER RNA; PROTEINASE; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BACTERIAL GENE; BIOENGINEERING; BIOLOGICAL MONITORING; BIOPROCESS; CELL FUNCTION; CELL GROWTH; CONCENTRATION RESPONSE; CONTROLLED STUDY; ESCHERICHIA COLI; FERMENTATION; GENE OVEREXPRESSION; GENETIC TRANSCRIPTION; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SYNTHESIS; RNA ANALYSIS; SACCHAROMYCES CEREVISIAE;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 0034693456     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20001020)70:2<217::AID-BIT11>3.0.CO;2-W     Document Type: Article

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