Utilising atomic force microscopy for the characterisation of nanoscale drug delivery systems

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 74, Issue 1, 2010, Pages 2-13

  Sitterberg, Johannes ^a   Özcetin, Aybike ^a   Ehrhardt, Carsten ^b   Bakowsky, Udo ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

AFM; Drug delivery systems; Gene vehicle; Liposomes; Nanoparticles; SFM

Indexed keywords

AZITHROMYCIN; LIDOCAINE; LIPOSOME; NANOPARTICLE; POLYGLACTIN;

ADHESION; ATOMIC FORCE MICROSCOPY; CHEMICAL STRUCTURE; CLINICAL EFFECTIVENESS; DEGRADATION KINETICS; DRUG DELIVERY SYSTEM; ELASTICITY; GENETIC TRANSFECTION; IMAGE ANALYSIS; NONVIRAL GENE DELIVERY SYSTEM; PARTICLE SIZE; PERMEABILITY BARRIER; PHYSICAL CHEMISTRY; REVIEW; SAMPLING;

DRUG DELIVERY SYSTEMS; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, ATOMIC FORCE; NANOSTRUCTURES; NANOTECHNOLOGY; PHARMACEUTICAL PREPARATIONS; TRANSFECTION;

EID: 72149119711     PISSN: 09396411     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejpb.2009.09.005     Document Type: Review

References (162)

1. Lipinski C.A. Drug-like properties and the causes of poor solubility and poor permeability. J. Pharm. Tox. Meth. 44 1 (2000) 235-249
2. Langer R. Drug delivery and targeting. Nature 392 6679 (1998) 5-10
3. Zhang L., Gu F.X., Chan J.M., Wang A.Z., Langer R.S., and Farokhzad O.C. Nanoparticles in medicine: therapeutic applications and developments. J. Clin. Pharm. Ther. 83 5 (2008) 761-769
4. Soppimath K.S., Aminabhavi T.M., Kulkarni A.R., and Rudzinski W.E. Biodegradable polymeric nanoparticles as drug delivery devices. J. Control. Release 70 1-2 (2001) 1-20
5. Allen T.M., and Cullis P.R. Drug delivery systems: entering the mainstream. Science 303 (2004) 1818-1822
6. Abrams I.M., and McBain J.W. A closed cell for electron microscopy. J. Appl. Phys. 15 (1944) 607-609
7. Swif J.A., and Brown A.C. An environmental cell for the examination of wet biological specimens at atmospheric pressure by transmission scanning electron microscopy. J. Phys. E 3 (1970) 924-926
8. Davies M., Brindley A., Chen X., Marlow M., Doughty S.W., Shrubb I., and Roberts C.J. Characterization of drug particle surface energetics and Youngs modulus by atomic force microscopy and inverse gas chromatography. Pharm. Res. 22 7 (2005) 1158-1166
9. Turner Y.T., Roberts C.J., and Davies M.C. Scanning probe microscopy in the field of drug delivery. Adv. Drug Deliv. Rev. 59 14 (2007) 1453-1473
10. Binnig G., and Rohrer H. Scanning tunnelling microscopy. Helv. Phys. Acta 55 (1982) 726-735
11. Zasadzinski J.A. Scanning tunneling microscopy with applications to biological surfaces. Biotechniques 7 2 (1989) 174-187
12. Binnig G., Quate C.F., and Gerber C. Atomic force microscope. Phys. Rev. Lett. 56 (1986) 930-933
13. Mecke A., Uppuluri S., Sassanella T.M., Lee D.K., Ramamoorthy A., Baker J.R., and Orr B.G. Direct observation of lipid bilayer disruption by poly (amidoamine) dendrimers. Chem. Phys. Lip. 132 (2004) 3-14
14. Pesen D., and Hoh J.H. Micromechanical architecture of the endothelial cell cortex. Biophys. J. 88 1 (2005) 670-679
15. Van Vliet K.J., and Hinterdorfer P. Probing drug-cell interactions. Nano Today 1 3 (2006) 18-25
16. Henderson E. Imaging of living cells by atomic force microscopy. Prog. Surf. Sci. 46 1 (1994) 39-60
17. Pereira R.d.S. Detection of the absorption of glucose molecules by living cells using atomic force microscopy. FEBS Lett. 475 1 (2000) 43-46
18. Dong G.H., Danying L., Weiguo C., Yunxu S., Lei J., Wanyun M., and Wang C. MAC mode atomic force microscopy studies of living samples, ranging from cells to fresh tissue. Ultramicroscopy 107 4-5 (2007) 299-307
19. Schneider S.W., Egan M.E., Jena B.P., Guggino W.B., Oberleithner H., and Geibel J.P. Continuous detection of extracellular ATP on living cells by using atomic force microscopy. PNAS 96 (1999) 12180-12185
20. Schneider S.W., Pagel P., Rotsch C., Danker T., Oberleithner H., Radmacher M., and Schwab A. Volume dynamics in migrating epithelial cells measured with atomic force microscopy. Pflugers Arch. Eur. J. Physiol. 439 3 (2000) 297-303
21. Radmacher M. Measuring the elastic properties of biological samples with the AFM. IEEE Eng. Med. Biol. Mag. 16 2 (1997) 47-57
22. Ohnesorge F.M., Horber J.K., Haberle W., Czerny C.P., Smith D.P., and Binnig G. AFM review study on pox viruses and living cells. Biophys. J. 73 4 (1997) 2183-2194
23. Li P., Yu F., Loehbach C., Muecklich F., Lehr C.M., and Bakowsky U. New methods of laser ablation patterning induces directional cell growth. IEEE Trans. NanoBiosci. 2 3 (2003) 138-145
24. Christ K., Wiedemann I., Bakowsky U., Sahl H.G., and Bendas G. The role of lipid II in membrane binding of and pore formation by nisin analyzed by two combined biosensor techniques. Biochim. Biophys. Acta. 1768 3 (2007) 694-704
25. Berquand A., Mingeot-Leclercq M., and Dufrene Y. Real-time imaging of drug-membrane interactions by atomic force microscopy. BBA-Biomembr. 1664 (2004) 198-205
26. Pereira R.d.S. Atomic force microscopy as a novel pharmacological tool. Biochem. Pharm. 62 8 (2001) 975-983
27. Danesh A., Davies M.C., Hinder S.J., Roberts C.J., Tendler S.J., Williams P.M., and Wilkins M.J. Surface characterization of aspirin crystal planes by dynamic chemical force microscopy. Anal. Chem. 72 15 (2000) 3419-3422
28. Danesh A., Connell S.D., Davies M.C., Roberts C.J., Tendler S.J.B., Williams P.M., and Wilkins M.J. An in situ dissolution study of aspirin crystal planes (1 0 0) and (0 0 1) by atomic force microscopy. Pharm. Res. 18 3 (2001) 299-303
29. Kneuer C., Sameti M., Bakowsky U., Schiestel T., Schirra H., Schmidt H., and Lehr C.M. Surface modified silica-nanoparticles can enhance transfection in vitro: a novel class of non-viral DNA vectors. Bioconj. Chem. 11 6 (2000) 926-932
30. Westedt U., Kalinowski M., Wittmar M., Merdan T., Unger F., Fuchs J., Schaller S., Bakowsky U., and Kissel T. Poly(vinyl alcohol)-graft-poly(lactide-co-glycolide) nanoparticles for local delivery of paclitaxel for restenosis treatment. J. Control. Release 119 1 (2007) 41-51
31. Simon M., Wittmar M., Bakowsky U., and Kissel T. Self-assembling nanoplexes from insulin and water-soluble branched polyesters, poly[(vinyl-3-(diethylamino)-propylcarbamate-co-(vinyl acetate)-co-(vinyl alcohol)]-graft-poly(l-lactic acid): A novel carrier for transmucosal delivery of peptides. Bioconj. Chem. 15 (2004) 841-849
32. Sanders G.H.W., Roberts C.J., Danesh A., Murray A.J., Price D.M., Davies M.C., Tendler S.J.B., and Wilkins M.J. Discrimination of polymorphic forms of a drug product by localized thermal analysis, short Communication. J. Microsc. 198 2 (2000) 77-81
33. Ward S., Perkins M., Zhang J., Roberts C.J., Madden C.E., Luk S.Y., Patel N., and Ebbens S.J. Identifying and mapping surface amorphous domains. Pharm. Res. 22 7 (2005) 1195-1202
34. Onoa G.B., Cervantes G., Moreno V., and Prieto M.J. Study of the interaction of DNA with cisplatin and other Pd(II) and Pt(II) complexes by atomic force microscopy. Nucl. Acids Res. 26 6 (1998) 1473-1480
35. Krautbauer R., Clausen-Schaumann H., and Gaub H.E. Cisplatin changes the mechanics of single DNA molecules. Angew. Chem. Int. Ed. 39 21 (2000) 3912
36. Danesh A., Chen X.Y., Davies M.C., Roberts C.J., Sanders G.H.W., Tendler S.J.B., Williams P.M., and Wilkins M.J. The discrimination of drug polymorphic forms from single crystals using atomic force microscopy. Pharm. Res. 17 7 (2000) 887-890
37. Thompson C., Davies M.C., Roberts C.J., Tendler S.J.B., and Wilkinson M.J. The effects of additives on the growth and morphology of paracetamol (acetaminophen) crystals. Int. J. Pharm. 280 1-2 (2004) 137-150
38. Burton W.K., Cabrera N., and Frank F.C. The growth of crystals and the equilibrium structure of their surfaces. Philos. Trans. Roy. Soc. Lond. Philos. Trans. A243 (1951) 299-358
39. Mahlin D., Berggren J., Alderborn G., and Engström S. Moisture-induced crystallisation of spray-dried amorphous lactose particles studied by atomic force microscopy. J. Pharm. Sci. 93 (2004) 29-37
40. Price R., and Young P.M. Visualization of the crystallisation of lactose from the amorphous state. J. Pharm. Sci. 93 (2004) 155-164
41. Wong J., Chilkoti A., and Moy V.T. Direct force measurements of the streptavidin-biotin interaction. Biomol. Eng. 16 1-4 (1999) 45-55
42. Radmacher M., Cleveland J.P., Fritz M., Hansma H.G., and Hansma P.K. Mapping interaction forces with the atomic force microscope. Biophys. J. 66 6 (1994) 2159-2165
43. Quist A.P., Bergman A.A., Reimann C.T., Oscarsson S.O., and Sundqvist B.U. Imaging of single antigens, antibodies, and specific immunocomplex formation by scanning force microscopy. Scan. Microsc. 9 2 (1995) 395-400
44. Florin E.L., Moy V.T., and Gaub H.E. Adhesion forces between individual ligand-receptor pairs. Science 264 5157 (1994) 415-417
45. Chen A., and Moy V.T. Cross-linking of cell surface receptors enhances cooperativity of molecular adhesion. Biophys. J. 78 6 (2000) 2814-2820
46. Pohl D.W., Fischer U.C., and Durig U.T. Scanning near-field optical microscopy (SNOM). J. Microsc. 152 (1988) 853-861
47. Price D.M., Reading M., Hammiche A., and Pollock H.M. Micro-thermal analysis: scanning thermal microscopy and localised thermal analysis. Int. J. Pharm. 192 1 (1999) 85-96
48. ® E100 using microthermal analysis. Pharm. Res. 20 1 (2003) 135-138
49. E. Meyer, H. Heinzelmann, in: Scanning Tunneling Microscopy II, Springer Ser. Surf. Sci., vol. 28, Springer, Berlin, Heidelberg, 1992, pp. 99-146.
50. Jones R., Pollock H., Geldart D., and Verlinden-Luts A. Frictional forces between cohesive powder particles studied by AFM. Ultramicroscopy 100 (2004) 59-78
51. Rasa M., Kuipers B., and Philipse A. Atomic force microscopy and magnetic force microscopy study of model colloids. J. Colloid Interf. Sci. 250 (2002) 303-315
52. Ge G., Han D., Lin D., Chu W., Sun Y., Jiang L., Ma W., and Wang C. MAC mode atomic force microscopy studies of living samples, ranging from cells to fresh tissue. Ultramicroscopy 107 4-5 (2007) 299-307
53. Mélin T., Diesinger H., Deresmes D., and Stiévenard D. Probing nanoscale dipole-dipole interactions by electric force microscopy. Phys. Rev. Lett. 92 (2004) 166101
54. Butt H.J., Cappella B., and Kappl M. Force measurements with the atomic force microscope: technique, interpretation and applications. Surf. Sci. Rep. 59 (2005) 1-152
55. Giessibl F., Hembacher S., Bielefeldt H., and Mannhart J. Subatomic features on the silicon (1 1 1) - (7 × 7) surface observed by atomic force microscopy. Science 289 (2000) 422-425
56. Shahin V., and Barrera N.P. Providing unique insight into cell biology via atomic force microscopy. Int. Rev. Cytol. 265 (2008) 227-252
57. Liang X., Mao G., and Ng K.Y.S. Effect of chain lengths of PEO-PPO-PEO on small unilamellar vesicles morphology and stability: an AFM investigation. J. Colloid Interf. Sci. 285 (2005) 360-372
58. Tarasova A., Griesser H.J., and Meagher L. AFM study of the stability of a dense affinity-bound liposome layer. Langmuir ACS J. Surf. Colloids Sci. 24 14 (2008) 7371-7377
59. Bakowsky H., Richter T., Kneuer C., Hoekstra D., Rothe U., Bendas G., Ehrhardt C., and Bakowsky U. Adhesion characteristics and stability assessment of lectin-modified liposomes for site-specific drug delivery. Biochim. Biophys. Acta. 1778 1 (2008) 242-249
60. Anabousi S., Kleemann E., Bakowsky U., Kissel T., Schmehl T., Gessler T., Seeger W., Lehr C.M., and Ehrhardt C. Effect of PEGylation on the stability of liposomes during nebulisation and in lung surfactant. J. Nanosci. Nanotechnol. 6 9-10 (2006) 3010-3016
61. Kleemann E., Schmehl T., Gessler T., Bakowsky U., Kissel T., and Seeger W. Iloprost-containing liposomes for aerosol application in pulmonary arterial hypertension: formulation aspects and stability. Pharm. Res. 24 2 (2007) 277-287
62. Schmitz I., Schreiner M., Friedbacher G., and Grasserbauer M. Phase imaging as an extension to tapping mode AFM for the identification of material properties on humidity-sensitive surfaces. Appl. Surf. Sci. 115 2 (1997)
63. Winkler R.G., Spatz J.P., Sheiko S., Moller M., Reineker P., and Marti O. Imaging material properties by resonant tapping-force microscopy: a model investigation. Phys. Rev. B. Condens. Matter. 54 12 (1996) 8908-8912
64. Lin W.C., Blanchette C.D., Ratto T.V., and Longo M.L. Lipid asymmetry in DLPC/DSPC-supported lipid bilayers: a combined AFM and fluorescence microscopy study. Biophys. J. 90 1 (2006) 228-237
65. Weiss B., Schaefer U.F., Zapp J., Lamprecht A., Stallmach A., and Lehr C.M. Nanoparticles made of fluorescence-labelled poly(L-lactide-co-glycolide): preparation, stability, and biocompatibility. J. Nanosci. Nanotechnol. 6 (2006) 3048-3056
66. Hinterdorfer P., Baumgartner W., Gruber H.J., Schilcher K., and Schindler H. Detection and localization of individual antibody-antigen recognition events by atomic force microscopy. Proc. Natl. Acad. Sci. USA 93 (1996) 3477-3481
67. Gould S.A., Burke K., and Hansma P.K. Simple theory for the atomic-force microscope with a comparison of theoretical and experimental images of graphite. Phys. Rev. B. Condens. Matter. 40 8 (1989) 5363-5366
68. Hansma H.G., Weisenhorn A.L., Edmundson A.B., Gaub H.E., and Hansma P.K. Atomic force microscopy: seeing molecules of lipid and immunoglobulin. Clin. Chem. 37 9 (1991) 1497-1501
69. Thundat T., Allison D.P., Warmack R.J., and Ferrell T.L. Imaging isolated strands of DNA molecules by atomic force microscopy. Ultramicroscopy 42-44 (1992) 1101-1106
70. Thundat T., Warmack R.J., Allison D.P., Bottomley L.A., Lourenco A.J., and Ferrell T.L. Atomic force microscopy of deoxyribonucleic acid strands adsorbed on mica: the effect of humidity on apparent width and image contrast. J. Vac. Sci. Technol. A10 (1992) 630-635
71. Thundat T., Allison D.P., Warmack R.J., Brown G.M., Jacobson K.B., Schrick J.J., and Ferrell T.L. Atomic force microscopy of DNA on mica and chemically modified mica. Scan. Microsc. 6 4 (1992) 911-918
72. Wong S.S., Joselevich E., Woolley A.T., Cheung C.L., and Lieber C.M. Covalently functionalized nanotubes as nanometre-sized probes in chemistry and biology. Nature 394 6688 (1998) 52-55
73. Hansma H.G., Vesenka J., Siegerist C., Keldermann G., Morrett H., Sinsheimer R.L., Elings V., Bustamante C., and Hansma P.K. Reproducible imaging and dissection of plasmid DNA under liquid with the atomic force microscopy. Science 256 (1992) 1180-1184
74. Hansma H.G., Sinsheimer R.L., Li M.Q., and Hansma P.K. Atomic force microscopy of single- and double-stranded DNA. Nucl. Acids Res. 20 14 (1992) 3585-3590
75. Hansma H.G., Bezanilla M., Zenhausern F., Adrian M., and Sinsheimer R.L. Atomic force microscopy of DNA in aqueous solutions. Nucl. Acids Res. 21 3 (1993) 505-512
76. Heckl W.M., Smith D.P.E., Binnig G., Klagges H., Hansch T.W., and Maddocks J. Two dimensional ordering of the DNA base Guanine observed with scanning tunneling microscopy. Proc. Nat. Acad. Sci. USA 88 (1991) 8003-8005
77. Ohlsson P.A., Tjärnhage T., Herbai E., Löfas S., and Puu G. Liposome and proteoliposome fusion onto solid substrates, studied using atomic force microscopy, quartz crystal microbalance and surface plasmon resonance. Biological activities of incorporated components. Bioelectrochem. Bioenergy 38 (1995) 137-148
78. Tillmann R.W., Hofmann U.G., and Gaub H.E. AFM-investigation of the molecular structure of films from a polymerizable two-chain lipid. Chem. Phys. Lipids 73 (1994) 81-89
79. Müller D.J., Schabert F.A., Büldt G., and Engel A. Imaging purple membrane in aqueous solutions at sub-nanometer resolution by atomic force microscopy. Biophys. J. 68 (1995) 1681-1686
80. Marti O., Elings V., Haugan M., Bracker C.E., Schneir J., Drake B., Gould S.A., Gurley J., Hellemans L., and Shaw K. Scanning probe microscopy of biological samples and other surfaces. J. Microsc. 152 (1988) 803-809
81. Zuhorn I.S., Bakowsky U., Polushkin E., Visser W.H., Stuart M.C.A., Engberts J.B.F.N., and Hoekstra D. Nonbilayer phase of lipoplex - membrane mixture determines endosomal escape of genetic cargo and transfection efficiency. Mol. Ther. 11 5 (2005) 801-810
82. Oberle V., Bakowsky U., Zuhorn I., and Hoekstra D. Lipoplex formation under equilibrium conditions reveals a three step mechanism. Biophys. J. 79 (2000) 1447-1454
83. Morris V.J. Biological applications of scanning probe microscopies. Prog. Biophys. Mol. Biol. 61 (1994) 131-185
84. Muller D.J., Amrein M., and Engel A. Adsorption of biological molecules to a solid support for scanning probe microscopy. J. Struct. Biol. 119 2 (1997) 172-188
85. Villarrubia J.S. Morphological estimation of tip geometry for scanned probe microscopy. Surf. Sci. 321 3 (1994) 287-300
86. Keller D., and Chung C.H. Imaging steep, high structures by scanning force microscopy with electron beam deposited tips. Surf. Sci. 268 (1992) 333-339
87. Micic M.., Chen A., Leblanc R.M., and Moy V.T. Scanning electron microscopy studies of protein-functionalized atomic force microscopy cantilever tips. Scanning 21 6 (1999) 394-397
88. Gu Y., Xie H., Gao J., Liu D., Williams C.T., Murphy C.J., and Ploehn H.J. AFM characterization of dendrimer-stabilized platinum nanoparticles. Langmuir 21 7 (2005) 3122-3131
89. Oliva M., Diez-Perez I., Gorostiza P., Lastra C., Oliva I., Caramella C., and Marino E. Self-assembly of drug-polymer complexes: a spontaneous nanoencapsulation process monitored by atomic force microscopy. J. Pharm. Sci. 92 1 (2002) 77-83
90. Moughton A.O., and O'Reilly R.K. Noncovalently connected micelles, nanoparticles, and metal-functionalized nanocages using supramolecular self-assembly. J. Am. Chem. Soc. 130 (2008) 8714-8725
91. Almofti M.R., Harashima H., Shinohara Y., Almofti A., Baba Y., and Kiwada H. Cationic liposome-mediated gene delivery: biophysical study and mechanism of internalization. Arch. Biochem. Biophys. 410 2 (2003) 246-253
92. Mao S., Neu M., Germershaus O., Merkel O., Sitterberg J., Bakowsky U., and Kissel T. Influence of polyethylene glycol chain length on the physicochemical and biological properties of poly(ethylene imine)-graft-poly(ethylene glycol) block copolymer/SiRNA polyplexes. Bioconj. Chem. 17 5 (2006) 1209-1218
93. Prabu P., Chaudhari A.A., Aryal A., Dharmaraj N., Park S.Y., Kim W.D., and Kim H.Y. In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell. J. Mater. Sci. Mater. Med. 19 5 (2008) 2157-2163
94. Leonenko Z., Finot E., and Amrein M. Adhesive interaction measured between AFM probe and lung epithelial type II cells. Ultramicroscope 107 10-11 (2007) 948-953
95. Shekunov B.Y., Chattopadhyay P., Tong H.H.Y., and Chow A.H.L. Particle size analysis in pharmaceutics: principles, methods and applications. Pharm. Res. 24 (2007) 203-227
96. Oikawa K., Kim H., Watanabe N., Shigeno M., Shirakawabe Y., and Yasuda K. Measuring the sizes of nanospheres on a rough surface by using atomic force microscopy and a curvature-reconstruction method. Sensor. Nanostruct. Ultramicroscopy Proceedings of the 8th Int. Conference on Scanning Probe Microscopy 107 10-11 (2007) 1061-1067
97. Roe G., McDonnell L., and Ghanem A. A method for measuring the size distribution of latex particles by scanning force microscopy. Ultramicroscope 100 3-4 (2004) 319-329
98. Lacava B.M., Azevedo R.B., Silva L.P., Lacava Z.G.M., Neto K.S., Buske N., Bakuzis A.F., and Morais P.C. Particle sizing of magnetite-based magnetic fluid using atomic force microscopy: a comparative study with electron microscopy and birefringence. Appl. Phys. Lett. 77 (2000)
99. 99mTechnetium-fluconazole nanocapsules. Int. J. Pharm. 349 (2008) 152-160
100. Wooley K.L. Shell crosslinked polymer assemblies: nanoscale constructs inspired from biological systems. J. Polym. Sci. Part A. Polym. Chem. 38 9 (2000) 1397-1407
101. Qi K., Ma Q., Remsen E., Clark Jr. C., and Wooley K. Determination of the bioavailability of biotin conjugated onto shell crosslinked (SCK) nanoparticles. J. Am. Chem. Soc. 126 21 (2004) 6599-6607
102. Zhang Q., Remsen E., and Wooley K. Shell cross-linked nanoparticles containing hydrolytically degradable, crystalline core domains. J. Am. Chem. S. 122 15 (2000) 3642-3651
103. Paik P., Kar K., Deva D., and Sharma A. Measurement of mechanical properties of polymer nanospheres by atomic force microscopy: effects of particle size. Micro Nano Lett. IET 2 3 (2007) 72-77
104. Stock W.H.R.R.S. J. Polym. Sci.: Polym. Phys. Ed. 23 (1985) 1393
105. Montasser I., Fessi H., and Coleman A.W. Atomic force microscopy imaging of novel type of polymeric colloidal nanostructures. Eur. J. Pharm. Biopharm. 54 3 (2002) 281-284
106. Chatterjee J., Haik Y., and Chen C. Polyethylene magnetic nanoparticle: a new magnetic material for biomedical applications. J. Mag. Mag. Mat. 246 3 (2002) 382-391
107. Packhaeuser C., and Kissel T. On the design of in situ forming biodegradable parenteral depot systems based on insulin loaded dialkylaminoalkyl-amine-poly(vinyl alcohol)-g-poly(lactide-co-glycolide) nanoparticles. J. Control. Release 123 (2007) 131-140
108. Germershaus O., Mao S., Sitterberg J., Bakowsky U., and Kissel T. Gene delivery using chitosan, trimethyl chitosan or polyethylenglycol-graft-trimethyl chitosan block copolymers: establishment of structure-activity relationships in vitro. J. Control. Release 125 (2008) 145-154
109. Dailey L.A., Kleemann E., Wittmar M., Gessler T., Schmehl T., Roberts C., Seeger W., and Kissel T. Surfactant-free, biodegradable nanoparticles for aerosol therapy based on the branched polyesters, DEAPA-PVAL-g-PLGA. Pharm. Res. 20 12 (2003) 2011-2020
110. Kneuer C., Ehrhardt C., Bakowsky H., Kumar M.N., Oberle V., Lehr C.M., Hoekstra D., and Bakowsky U. The influence of physicochemical parameters on the efficacy of non-viral DNA transfection complexes: a comparative study. J. Nanosci. Nanotechnol. 6 (2006) 2776-2782
111. Kumar M.N.V.R., Bakowsky U., and Lehr C.M. Preparation and characterization of cationic PLGA nanospheres as DNA carriers. Biomaterials 25 (2004) 1771-1777
112. Görner T., Gref R., Michenot D., Sommer F., Tran M.N., and Dellacherie E. Lidocaine-loaded biodegradable nanospheres. I. Optimization of the drug incorporation into the polymer matrix. J. Control. Release 57 3 (1999) 259-268
113. Yuan B., and Wicks D.A. Thermotropic color changing nanoparticles prepared by encapsulating blue polystyrene particles with a poly-N-isopropylacrylamide gel. J. Appl. Poly. Sci. 105 (2007) 446-452
114. Ramesh S., Cohen Y., Aurbach D., and Gedanken A. Atomic force microscopy investigation of the surface topography and adhesion of nickel nanoparticles to submicrospherical silica. Chem. Phys. Lett. 287 (1998) 461-467
115. ®): PLGA nanoparticles containing vitamin E TPGS. J. Control. Release 86 (2003) 33-48
116. Feng S.S., Mu L., Win K.Y., and Huang G. Nanoparticles of biodegradable polymers for clinical administration of paclitaxel. Curr. Med. Chem. 11 4 (2004) 413-424
117. Wang C., Zhang Y., Seng H.S., and Ngo L.L. Nanoparticle-assisted micropatterning of active proteins on solid substrate. Biosens. Bioelect. 21 (2006) 1638-1643
118. Bowen W.R., and Williams P.M. Dynamic ultrafiltration model for proteins: a colloidal interaction approach. Biotech. Bioeng. 50 (1996) 125-135
119. Cai C., Bakowsky U., Rytting E., Schaper A.K., and Kissel T. Charged nanoparticles as protein delivery systems: a feasibility study using lysozyme as model protein. Eur. J. Pharm. Biopharm. 69 1 (2008) 31-42
120. Schäfer J., Sitterberg J., Ehrhardt C., Ravi Kumar M.N.V., and Bakowsky U. A new drug vehicle - lipid coated biodegradable nanoparticles. Adv. Sci. Technol. 57 (2008) 148-153
121. Yip C.M., Brader L.M., Frank B.H., DeFelippis M.R., and Ward M.D. Structural studies of a crystalline insulin analog complex with protamine by atomic force microscopy. Biophys. J. 78 (2000) 466-473
122. Mamot C., Drummond D.C., Hong K., Kirpotin D.B., and Park J.W. Liposome-based approaches to overcome anticancer drug resistance. Drug Resist. Updates 6 (2003) 271-279
123. Ramachandran S., Quist A.P., Kumar S., and Lal R. Cisplatin nanoliposomes for cancer therapy: AFM and fluorescence imaging of cisplatin encapsulation, stability, cellular uptake, and toxicity. Langmuir 22 19 (2006) 8156-8162
124. Bergers J.J., Ten Hagen T.L.M., Van Etten E.W.M., and Bakker-Woudenberg I.A.J.M. Liposomes as delivery systems in the prevention and treatment of infectious diseases. Pharm. World Sci. 17 (2005) 1-11
125. Niesman M.R. The use of liposomes as drug carriers in ophthalmology. Crit. Rev. Ther. Drug Carr. Syst. 9 1 (1992) 1-38
126. Gaspar M., Bakowsky U., and Ehrhardt C. Inhaled liposomes - current strategies and future challenges. J. Biomed. Nanotechnol. 4 3 (2008) 245-257
127. Gaspar M.M., Boerman O.C., Laverman P., Corvo M.L., Storm G., and Cruz M.E. Enzymosomes with surface-exposed superoxide dismutase: in vivo behaviour and therapeutic activity in a model of adjuvant arthritis. J. Control. Release 117 2 (2007) 186-195
128. Anabousi S., Bakowsky U., Schneider M., Huwer H., Lehr C.M., and Ehrhardt C. In vitro assessment of transferrin-conjugated liposomes as drug delivery systems for inhalation therapy of lung cancer. Eur. J. Pharm. Sci. 29 5 (2006) 367-374
129. Sharma G., Anabousi S., Ehrhardt C., and Ravi Kumar M.N. Liposomes as targeted drug delivery systems in the treatment of breast cancer. J. Drug. Target. 14 5 (2006) 301-310
130. Anabousi S., Laue M., Lehr C.M., Bakowsky U., and Ehrhardt C. Assessing transferrin modification of liposomes by atomic force microscopy and transmission electron microscopy. Eur. J. Pharm. Biopharm. 60 2 (2005) 295-303
131. Torchilin V.P. Recent advances with liposomes as pharmaceutical carriers. Nat. Rev. Drug Discov. 4 (2005) 145-159
132. Vermette P., Meagher L., Gagnon E., Griesser H.J., and Doillon C.J. Immobilized liposome layers for drug delivery applications: inhibition of angiogenesis. J. Control. Release 80 1-3 (2002) 179-195
133. Carmo V.A., De Oliveira M.C., Reis E.C., Guimarães T.M., Vilela J.M., Andrade M.S., Michalick M.S., and Cardoso V.N. Physicochemical characterization and study of in vitro interactions of pH-sensitive liposomes with the complement system. J. Liposome Res. 18 1 (2008) 59-70
134. Bendas G., Krause A., Bakowsky U., Vogel J., and Rothe U. Targetability of novel immunoliposomes prepared by a new antibody conjugation technique. Int. J. Pharm. 181 1 (1999) 79-93
135. Nakano K., Tozuka Y., Yamamoto H., Kawashima Y., and Takeuchi H.A. A novel method for measuring rigidity of submicron-size liposomes with atomic force microscopy. Int. J. Pharm. 355 1-2 (2008) 203-209
136. Sakai K., Tomizawa H., Tsuchiya K., Ishida N., Sakai H., and Abe M. Characterizing the structural transition of cationic DPPC liposomes from the approach of TEM, SAXS and AFM measurements. Colloids. Surf. B. Biointerf. 67 1 (2008) 73-78
137. Liang X., Mao G., and Ng K.Y. Mechanical properties and stability measurement of cholesterol containing liposomes on mica by atomic force microscopy. J. Colloid. Interf. Sci. 278 1 (2004) 53-62
138. Ruozi B., Tosi G., Forni F., Fresta M., and Vandelli M.A. Atomic force microscopy and photon correlation spectroscopy: two techniques for rapid characterization of liposomes. Eur. J. Pharm. Sci. 25 1 (2005) 81-89
139. Berquand A., Fa N., Dufrêne Y.F., and Mingeot-Leclercq M.P. Interaction of the macrolide antibiotic azithromycin with lipid bilayers: effect on membrane organization, fluidity, and permeability. Pharm. Res. 22 3 (2005) 465-475
140. Fa N., Lins L., Courtoy P.J., Dufrêne Y., Van Der Smissen P., Brasseur R., Tyteca D., and Mingeot-Leclercq M.P. Decrease of elastic moduli of DOPC bilayers induced by a macrolide antibiotic, azithromycin. Biochim. Biophys. Acta. 1768 7 (2007) 1830-1838
141. Zuhorn I.S., Oberle V., Visser W.H., Engberts J.B., Bakowsky U., Polushkin E., and Hoekstra D. Phase behavior of cationic amphiphiles and their mixtures with helper lipid influences lipoplex shape, DNA translocation, and transfection efficiency. Biophys. J. 83 4 (2002) 2096-2108
142. Marty R., N'soukpoé-Kossi C.N., Charbonneau D., Weinert C.M., Kreplak L., and Tajmir-Riahi H.A. Structural analysis of DNA complexation with cationic lipids. Nucl. Acids Res. (2008) (Epub ahead of print)
143. Kawaura C., Noguchi A., Furuno T., and Nakanishi M. Atomic force microscopy for studying gene transfection mediated by cationic liposomes with a cationic cholesterol derivative. FEBS Lett. 421 1 (1998) 69-72
144. Zhao Y.Z., Luo Y.K., Liang H.D., Mei X.G., Tang J., Lu C.T., Zhang Y., and Lin Q. Comparing transfection efficiency and safety for antisense oligodeoxyribonucleotide between phospholipids-based microbubbles and liposomes. J. Drug Target. 4 10 (2006) 687-693
145. Wolfert M.A., and Seymour L.W. Atomic force microscopic analysis of the influence of the molecular weight of poly(l)lysine on the size of polyelectrolyte complexes formed with DNA. Gene Ther. 3 (1996) 269-273
146. Wolfert M.A., Schacht E.H., Toncheva V., Ulbrich K., Nazarova O., and Seymour L.M. Characterization of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers. Hum. Gene Ther. 7 (1996) 2123-2133
147. Werth S., Urban-Klein B., Dai L., Hobel S., Grzelinski M., Bakowsky U., Czubayko F., and Aigner A. A low molecular weight fraction of polyethylenimine (PEI) displays increased transfection efficiency of DNA and siRNA in fresh or lyophilized complexes. J Control. Release 112 2 (2006) 257-270
148. Oster C.G., Wittmar M., Bakowsky U., and Kissel T. DNA nano-carriers from biodegradable cationic branched polyesters are formed by a modified solvent displacement method. J. Control. Release 111 3 (2006) 371-381
149. Merdan T., Kunath K., Petersen H., Bakowsky U., Voigt K.H., Kopecek J., and Kissel T. PEGylation of poly(ethylene imine) affects stability of complexes with plasmid DNA under in vivo conditions in a dose-dependent manner after intravenous injection into mice. Bioconjug. Chem. 16 4 (2005) 785-792
150. Merdan T., Callahan J., Petersen H., Kunath K., Bakowsky U., Kopeckova P., Kissel T., and Kopecek J. Pegylated polyethylenimine-Fab' antibody fragment conjugates for targeted gene delivery to human ovarian carcinoma cells. Bioconj. Chem. 14 5 (2003) 989-996
151. Chim Y.T., Lam J.K., Ma Y., Armes S.P., Lewis A.L., Roberts C.J., Stolnik S., Tendler S.J., and Davies M.C. Structural study of DNA condensation induced by novel phosphorylcholine-based copolymers for gene delivery and relevance to DNA protection. Langmuir 21 8 (2005) 3591-3598
152. Neu M., Sitterberg J., Bakowsky U., and Kissel T. Stabilized nanocarriers for plasmids based upon cross-linked poly(ethylene imine). Biomacromolecules 7 12 (2006) 3428-3438
153. Bakowsky U., and Sitterberg J. Understanding nanoscale drug delivery systems. Glatt Int. Times. 23 (2007) 19-23
154. Martin A.L., Davies M.C., Rackstraw B.J., Roberts C.J., Stolnik S., Tendler S.J.B., and Williams P.M. Observation of DNA-polymer condensate formation in real time at a molecular level. FEBS Lett. 480 (2000) 106-112
155. Abdelhady H.G., Allen S., Davies M.C., Roberts C.J., Tendler S.J.B., and Williams P.M. Direct real-time molecular scale visualisation of the degradation of condensed DNA complexes exposed to DNase I. Nucl. Acids Res. 31 14 (2003) 4001-4005
156. Grzelinski M., Urban-Klein B., Martens T., Lamszus K., Bakowsky U., Höbel S., Czubayko F., and Aigner A. RNA interference-mediated gene silencing of pleiotrophin through polyethylenimine-complexed small interfering RNAs in vivo exerts antitumoral effects in glioblastoma xenografts. Human Gene Ther. 17 7 (2006) 751-766
157. Pennadam S.S., Ellis J.S., Lavigne M.D., Górecki D.C., Davies M.C., and Alexander C. Synthesis and characterization of variable-architecture thermosensitive polymers for complexation with DNA. Langmuir 23 1 (2007) 41-49
158. Kleemann E., Dailey L.A., Abdelhady H.G., Gessler T., Schmehl T., Roberts C.J., Davies M.C., Seeger W., and Kissel T. Modified polyethylenimines as non-viral gene delivery systems for aerosol gene therapy: investigations of the complex structure and stability during air-jet and ultrasonic nebulization. J. Control. Release 100 3 (2004) 437-450
159. Peetla C., and Labhasetwar V. Biophysical characterization of nanoparticle-endothelial model cell membrane interactions. Mol. Pharm. 5 3 (2008) 418-429
160. Leroueil P.R., Berry S.A., Duthie K., Han G., Rotello V.M., McNerny D.Q., Baker Jr. J.R., Orr B.G., and Holl M.M. Wide varieties of cationic nanoparticles induce defects in supported lipid bilayers. Nano Lett. 8 (2008) 420-424
161. Kanno S., Furuyama A., and Hirano S. A murine scavenger receptor MARCO recognizes polystyrene nanoparticles. Toxicol. Sci. 97 2 (2007) 398-406
162. Pan Y.L., Cai J.Y., Qin L., and Wang H. Atomic force microscopy-based cell nanostructure for ligand-conjugated quantum dot endocytosis. Acta. Biochim. Biophys. Sin (Shanghai) 38 9 (2006) 646-652