Atomic force microscopic analysis of the influence of the molecular weight of poly(L)lysine on the size of polyelectrolyte complexes formed with DNA

Gene Therapy

Volumn 3, Issue 3, 1996, Pages 269-273

  Wolfert, M A ^a   Seymour, L W ^a  

^a UNIVERSITY DEPARTMENT OF MEDICINE   (United Kingdom)

Author keywords

Atomic force microscopy; Block copolymers; Poly(L)lysine; Self assembly; Synthetic vectors

Indexed keywords

DNA; POLYELECTROLYTE; POLYLYSINE;

ARTICLE; ATOMIC FORCE MICROSCOPY; COMPLEX FORMATION; CYTOTOXICITY; DNA PROTEIN COMPLEX; EXPRESSION VECTOR; MOLECULAR WEIGHT; PRIORITY JOURNAL;

CELL SURVIVAL; DNA; ELECTROLYTES; GENETIC VECTORS; HUMANS; MICROSCOPY, ATOMIC FORCE; MOLECULAR WEIGHT; POLYLYSINE; TUMOR CELLS, CULTURED;

EID: 0030005946     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Sikora K. Gene therapy - heralding a new era. Gene Therapy 1994; 1: 1-2.
2. Farhood H et al. Cationic liposomes for direct gene transfer in therapy of cancer and other diseases. In: Huber BE, Lazo JS (eds). Gene Therapy for Neoplastic Diseases Ann NY Acad Sci 1994; 716: 23-35.
3. Remy J-S et al. Targeted gene transfer into hepatoma cells with lipopolyamine-condensed DNA parades presenting galactose ligands: a stage towards artificial viruses. Proc Natl Acad Sci USA 1994; 92: 1744-1748.
4. Zhu N, Liggitt D, Liu Y, Debs R. Systemic gene expression after intravenous DNA delivery into adult mice. Science 1993; 261: 209-211.
5. Logan JJ et al. Cationic lipids for reporter gene and CFTR transfer to rat pulmonary epithelium. Gene Therapy 1995; 2: 38-49.
6. Sternberg B, Sorgi FL, Huang L. New structures in complex formation between DNA and cationic liposomes visualized by freeze-fracture electron microscopy. FEBS Lett 1994; 356: 361-366.
7. Jolly D. Viral vector systems for gene therapy. Cancer Gene Ther 1994; 1: 51-64.
8. Flotte TR et al. An improved system for packaging recombinant adeno-associated virus vectors capable of in vivo transduction. Gene Therapy 1995; 2: 29-37.
9. Curiel DT. High efficiency gene transfer mediated by adenovirus-polylysine-DNA complexes. In: Huber BE, Lazo JS (eds). Gene Therapy for Neoplastic Diseases. Ann NY Acad Sci 1994; 716: 36-58.
10. Seymour LW. Passive tumor targeting of soluble macromolecules and drug conjugates. Crit Rev Ther Drug Carrier Syst 1992; 9: 135-187.
11. Perales JC et al. Gene transfer in vivo: sustained expression and regulation of genes introduced into the liver by receptor-targeted uptake. Proc Natl Acad Sci USA 1994; 91: 4086-1090.
12. Wagner E, Cotten M, Foisner R, Birnstiel ML. Transferrin-polycation-DNA complexes: the effect of polycations on the structure of the complex and DNA delivery to cells. Proc Natl Acad Sci USA 1991; 88: 4255-4259.
13. Cotten M, Wagner E, Birnstiel ML. Receptor-mediated transport of DNA into eukaryotic cells. Methods Enzymol 1993; 217: 618-644.
14. Batra RK, Berschneider H, Curiel DT. Molecular conjugate vectors mediate efficient gene transfer into gastrointestinal epithelial cells. Cancer Gene Ther 1994; 1: 185-192.
15. Wagner E, Curiel D, Cotten M. Delivery of drugs, proteins and genes into cells using transferrin as a ligand for receptor-mediated endocytosis. Adv Drug Deliv Rev 1994; 14: 113-135.
16. Seymour LW. Synthetic polymers with intrinsic anticancer activity. J Bioact Compat Polym 1991; 6: 178-216.
17. Wolfert MA. Development of self-assembling vectors for gene therapy, MSc thesis, University of Birmingham, UK, 1994.
18. Sgouras D, Duncan R. Methods for the evaluation of biocompatibility of soluble synthetic polymers which have potential for biomedical use: 1 - Use of the tetrazolium-based colorimetric assay (MTT) as a preliminary screen for evaluation of in vitro cytotoxicity. J Mat Sci: Mat Med 1990; 1: 61-68.