Kinetic studies of constitutive human granulocyte-macrophage colony stimulating factor (hGM-CSF) expression in continuous culture of Pichia pastoris

Biotechnology Letters

Volumn 29, Issue 12, 2007, Pages 1903-1908

  Khasa, Yogender Pal ^a   Khushoo, Amardeep ^a   Srivastava, Leena ^a   Mukherjee, K J ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

Constitutive expression; Continuous culture; GAP Promoter; Human granulocyte macrophage colony stimulating factor; Pichia pastoris

Indexed keywords

BIOMASS; CONCENTRATION (PROCESS); FEEDBACK; GENETIC ENGINEERING; KINETICS; VOLUMETRIC ANALYSIS;

CONSTITUTIVE EXPRESSION; CONTINUOUS CULTURE; GAP PROMOTER; HUMAN GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR; PICHIA PASTORIS;

MACROPHAGES;

GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR;

ARTICLE; BIOMASS; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; GROWTH, DEVELOPMENT AND AGING; HUMAN; KINETICS; METABOLISM; METHODOLOGY; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BIOMASS; CULTURE MEDIA; CULTURE TECHNIQUES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HUMANS; KINETICS; PICHIA;

PICHIA PASTORIS;

EID: 35748948039     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9473-8     Document Type: Article

References (16)

1. Armitage JO (1998) Emerging applications of recombinant human granulocyte-macrophage colony-stimulating factor. Blood 92:4491-4508
2. Bhattacharya SK, Dubey AK (1995) Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene. Biotechnol Lett 17:1155-1160
3. Bhattacharya P, Pandey G, Shrivastva P, Mukherjee KJ (2005) Combined effect of protein fusion and signal sequence greatly enhances the production of recombinant hGM-CSF in Escherichia coli. Mol Biotechnol 30:103-116
4. Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24:45-66
5. Cos O, Ramon R, Montesinos JL, Valero F (2006) Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A Review. Microb Cell Fact 5:17
6. D'Anjou MC, Daugulis AJ (2000) Mixed-feed exponential feeding for fed-batch culture of recombinant methlotrophic yeast. Biotechnol Lett 22:341-346
7. Goodrick JC, Xu M, Finnegan R, Schilling BM, Schiavi S, Hoppe H, Wan NC (2001) High-level expression and stabilization of recombinant human chitinase produced in a continuous constitutive Pichia pastoris expression system. Biotechnol Bioeng 74:492-497
8. Jahic M, Rotticci-Mulder JC, Martinelle M, Hult K, Enfors SO (2002) Modeling of growth and energy metabolism of Pichia pastoris producing a fusion protein. Bioprocess Biosyst Eng 24:385-393
9. Murray WD, Duff SJB (1989) Induction and stability of alcohol oxidase in the methylotrophic yeast Pichia pastoris. Appl Microbiol Biotechnol 32:95-100
10. Nakano A, Lee CY, Yoshida A, Matsumoto T, Shiomi N, Katoh S (2006) Effects of methanol feeding methods on chimeric alpha-amylase expression in continuous culture of Pichia pastoris. J Biosci Bioeng 10:227-231
11. Pal Y, Khushoo A, Mukherjee KJ (2006) Process optimization of constitutive human granulocyte-macrophage colony-stimulating factor (hGM-CSF) expression in Pichia pastoris fed-batch culture. Appl Microbiol Biotechnol 69:650-657
12. Pirt SJ (1982) Maintenance energy - A general-model for energy-limited and energy-sufficient growth. Arch Microbiol 133:300-302
13. Shin CS, Hong MS, Kim DY, Shin HC, Lee J (1998) Growth-associated synthesis of recombinant human glucagon and human growth hormone in high-cell-density cultures of Escherichia coli. Appl Microbiol Biotechnol 49:364-370
14. Waterham HR, Digan ME, Koutz PJ, Lair SV, Cregg JM (1997) Isolation of the Pichia pastoris glyceraldehyd-3-phospate dehydrogenase gene and regulation and use of its promotor. Gene 186:37-44
15. Zhang W, Liu CP, Inan M, Meagher MM (2004) Optimization of cell density and dilution rate in Pichia pastoris continuous fermentation for production of recombinant proteins. J Ind Microbiol Biotechnol 31:330-334
16. Zhang AL, Zhang TY, Luo JX, Chen SC, Guan WJ, Fu CY, Peng SQ, Li HL (2007) Constitutive expression of human angiostatin in Pichia pastoris by high cell density culture. J Ind Microbiol Biotechnol 34:117-122