Translation elongation factor 1-α gene from Pichia pastoris: Molecular cloning, sequence, and use of its promoter

Applied Microbiology and Biotechnology

Volumn 74, Issue 3, 2007, Pages 601-608

  Ahn, Jungoh ^a   Hong, Jiyeon ^a   Lee, Hyeokweon ^a   Park, Myongsoo ^a   Lee, Eungyo ^a   Kim, Chunsuk ^a   Choi, Euisung ^a   Jung, Joonki ^a   Lee, Hongweon ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Constitutive promoter; Pichia pastoris; Translation elongation factor

Indexed keywords

CONSTITUTIVE PROMOTER; GENE ENCODING; PICHIA PASTORIS; TRANSLATION ELONGATION FACTOR;

AMINO ACIDS; BACTERIOLOGY; GENES; GLUCOSE; MOLECULAR DYNAMICS; PROTEINS; YEAST;

CLONING;

CELL PROTEIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; TRANSLATION ELONGATION FACTOR 1 ALPHA; UNCLASSIFIED DRUG;

AMINO ACID; GENE EXPRESSION; GLUCOSE; MOLECULAR ANALYSIS; PEPTIDE; PROTEIN; YEAST;

ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; FUNGAL GENE; FUNGUS GROWTH; GENE EXPRESSION; GENE FUNCTION; GENE SEQUENCE; INSTRUMENTAL CONDITIONING; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PROMOTER REGION; REPORTER GENE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; FUNGAL PROTEINS; GENE EXPRESSION; GENES, REPORTER; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); LIPASE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OPEN READING FRAMES; PEPTIDE ELONGATION FACTOR 1; PICHIA; PROMOTER REGIONS (GENETICS); SACCHAROMYCES; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS); PICHIA PASTORIS;

EID: 33847133851     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0698-6     Document Type: Article

References (31)

1. Ahn JO, Choi ES, Lee HW, Hwang SH, Kim CS, Jang HW, Haam SJ, Jung JK (2004) Enhanced secretion of Bacillus stearothermophilus L1 lipase in Saccharomyces cerevisiae by translational fusion to cellulose-binding domain. Appl Microbiol Biotechnol 64:833-839
2. Arocho A, Chen B, Ladanyi M, Pan Q (2006) Validation of the 2-DeltaDeltaCt calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts. Diagn Mol Pathol 15:56-61
3. Axelos M, Bardet C, Liboz T, Thai ALV, Curie C, Lescure B (1989) The gene family encoding the Arabidopsis thaliana translation elongation factor EF-1α: molecular cloning, characterization and expression. Mol Gen Genet 219:106-112
4. Bieche I, Olivi M, Champeme MH, Vidaud D, Lidereau R, Vidaud M (1998) Novel approach to quantitative polymerase chain reaction using real-time detection: application to the detection of gene amplification in breast cancer. Int J Cancer 78:661-666
5. Cereghino GPL, Cereghino J, ILgen C, Cregg JM (2002) Production of recombinant proteins in fermentor cultures of the yeast Pichia pastoris. Curr Opin Biotechnol 13:329-332
6. Da Silva NA, Bailey JE (1991) Influence of plasmid origin and promoter strength in fermentations of recombinant yeast. Biotechnol Bioeng 37:318-324
7. Denis CL, Ferguson J, Young ET (1983) mRNA levels for fermentative alcohol dehydrogenase of Saccharomyces cerevisiae decrease upon growth on a non-fermentable carbon source. J Biol Chem 258:1165-1171
8. Doring F, Klapper M, Thesis S, Daniel H (1998) Use of the glyceraldehyde-3-phosphate dehydrogenase promoter for the production of functional mammalian membrane transport protein in the yeast Pichia pastoris. Biochem Biophys Res Commun 250:531-535
9. Doring F, Klapper M, Thesis S, Daniel H (2000) Production of functional mammalian membrane transport proteins in the yeast Pichia pastoris. In: Invitrogen Newsletter expressions 7.4, June 2000, Research News pGAPZ Pichia pastoris expression system
10. Heo JH, Hong KH, Cho EY, Kim MW, Kim JY, Kim CH, Rhee SK, Kang HA (2003) Properties of the Hansenula polymorpha-derived constitutive GAP promoter, assessed using an HSA reporter gene. FEMS Yeast Res 4:175-184
11. Kitamoto N, Matsui J, Kawai Y, Kato A, Yoshino S, Ohmiya K, Tsukagoshi N (1998) Utilization of the TEF1-α gene (TEF1) promoter for expression of polygalacturonase genes, pgaA and pgaB, in Aspergillus oryzae. Appl Microbiol Biotechnol 50:85-92
12. Kobayashi K, Kuwaes S, Ohya T (2000) Addition of oleic acid increases expression of recombinant human serum albumin by the AOX2 promoter in Pichia pastoris. J Biosci Bioeng 89:479-484
13. Koller A, Valesco J, Subramani S (2000) The CUP1 promoter of Saccharomyces cerevisiae is inducible by copper in Pichia pastoris. Yeast 16:651-656
14. Kozak M (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:292-293
15. Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22:249-270
16. Mumberg D, Müller R, Funk M (1995) Yeast vectors for the controlled expression of heterologous proteins in difference genetic backgrounds. Gene 156:119-122
17. Müller S, Thomas S, Peter KH, Henrik D (1998) Comparison of expression systems in the yeast Saccharomyces cerevisiae, Hansenula polymorpha, Kluyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast 14:1267-1283
18. Nakari-Setala T, Penttila ME (1995) Production of Trichoderma reesei cellulases on glucose containing media. Appl Environ Microbiol 61:3650-3655
19. Nakari T, Alatalo E, Penttila ME (1993) Isolation of Trichoderma reesei genes highly expressed on glucose-containing media: characterization of the TEF1 gene encoding translation elongation factor 1 alpha. Gene 136:313-318
20. Pal Y, Khushoo A, Mukherjee KJ (2005) Process optimization of constitutive human granulocyte-macrophage colony-stimulating factor (hGM-CSF) expression in Pichia pastoris fed-batch culture. Appl Microbiol Biotechnol 69(6):650-657
21. Resina D, Scorer C, Valero F, Ferrer P (2004) Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. J Biotechnol 109:103-113
22. Romanos M, Scorer C, Sreekrishna K, Clare J (1998) The generation of multicopy recombinant strains. In: Higgins DR, Cregg JM (eds) Pichia protocols. Human Press, Totowa, NJ, USA, pp 55-72
23. Sambrook J, Russel DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
24. Schmitt ME, Brown TA, Trumpower BL (1990) A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res 18:3091-3092
25. Sears IB, O'Connor J, Rossanese OW, Glick BS (1998) A versatile set of vectors for constitutive gene expression in Pichia pastoris. Yeast 14:783-790
26. Slobin LI (1980) The role of eukaryotic elongation factor Tu in protein synthesis. Eur J Biochem 110:555-563
27. Steiner S, Philippsen P (1994) Sequence and promoter analysis of the highly expressed TEF gene of the filamentous fungus Ashbya gossypii. MGG Mol Gen Genet 242:263-271
28. Triglia T (2000) Inverse PCR (IPCR) for obtaining promoter sequence. In: Tymms MJ (ed) Transcription factor protocols. Humana Press, Totowa, NJ, USA, pp 79-84
29. Tschopp JF, Brust PF, Cregg JM, Stillman CA, Gingeras TR (1987) Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res 15:3859-3876
30. Waterham HR, Digan ME, Koutz PJ, Lair SV, Cregg V (1997) Isolation of the Pichia pastoris glyceraldehyde-3-phophate dehydrogenase gene and regulation and use of its promoter. Gene 186:37-44
31. Wu JM, Chieng LL, Hsu TA, Lee CK (2003) Sequential expression of recombinant proteins and their separate recovery from a Pichia pastoris cultivation. Biochem Eng J 16:9-16