Combined use of GAP and AOX1 promoter to enhance the expression of human granulocyte-macrophage colony-stimulating factor in Pichia pastoris

Enzyme and Microbial Technology

Volumn 33, Issue 4, 2003, Pages 453-459

  Wu, J M ^a   Lin, J C ^a   Chieng, L L ^a   Lee, C K ^a   Hsu, T A ^b,c  

^a NATIONAL TAIWAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Taiwan)

^b NATIONAL HEALTH RESEARCH INSTITUTES   (Taiwan)

^c NATIONAL TSING HUA UNIVERSITY   (Taiwan)

Author keywords

AOX1 promoter; GAP promoter; Glycoprotein; hGM CSF; P. pastoris

Indexed keywords

BIOCHEMICAL ENGINEERING; CELLS; CONCENTRATION (PROCESS); ENZYME KINETICS;

MACROPHAGES;

PROTEINS;

ALCOHOL OXIDASE 1; CASAMINO ACID; FUNGAL ENZYME; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR; UNCLASSIFIED DRUG;

BIOTECHNOLOGY; CONFERENCE PAPER; CONTROLLED STUDY; EXPRESSION VECTOR; FUNGAL STRAIN; FUNGUS CULTURE; GENE CASSETTE; GENE EXPRESSION REGULATION; HETEROLOGOUS EXPRESSION; NONHUMAN; PICHIA PASTORIS; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN INDUCTION; PROTEIN SECRETION;

PICHIA; PICHIA PASTORIS;

EID: 0042932426     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(03)00147-9     Document Type: Conference Paper

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