Effect of glycosylation modification (N-Q-108I → N-Q-108T) on the freezing stability of recombinant chicken cystatin overexpressed in Pichia pastoris X-33

Journal of Agricultural and Food Chemistry

Volumn 50, Issue 19, 2002, Pages 5313-5317

  Jiang, Shann Tzong ^a   Chen, Gen Hung ^a   Tang, Shye Jye ^a   Chen, Ching San ^b  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

^b INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

Author keywords

Glycosylation modification; Overexpression; P. pastoris; Recombinant cystatin

Indexed keywords

COMPLEMENTARY DNA; CYSTATIN; MUTANT PROTEIN; PAPAIN; RECOMBINANT CYSTATIN; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL MODIFICATION; CHICKEN; COMPETITIVE INHIBITION; CONTROLLED STUDY; ENZYME INHIBITION; FREEZING; GENE OVEREXPRESSION; INHIBITION KINETICS; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PROTEIN GLYCOSYLATION; PROTEIN STABILITY;

ANIMALS; CHICKENS; CYSTATINS; DRUG STABILITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FREEZING; GENE EXPRESSION; GLYCOSYLATION; PICHIA; RECOMBINANT PROTEINS;

GALLUS GALLUS; PICHIA; PICHIA PASTORIS; VECTORS;

EID: 0037063435     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf0200321     Document Type: Article

References (34)

1. Cystatin, a protein inhibitor of cysteine proteinase. Improved purification from egg white, characterization, and detection in chicken serum
2. The cystatins: A new class of peptidase inhibitors
3. Ficin and papain inhibitor from chicken egg white
4. The cystatins: Protein inhibitors of cysteine proteinases
5. Crystallization of chicken egg white cystatin, a low molecular weight protein inhibitor of cysteine proteinases, and preliminary x-ray diffraction data
6. The 2.0 Å x-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases
7. Effect of papain inhibitor from chicken egg white on cathepsin B1
8. Interaction of chicken cystatin with inactivated papains
9. Interaction between chicken cystatin and the cysteine proteinases actinidin, chymopapain A, and ficin
10. Kinetics of binding of chicken cystatin to papain
11. Inhibition of cysteine proteinases and dipeptidyl peptidase I by egg-white cystatin
12. Effect of glycosylation on the stability of α1-antitrypsin toward urea denaturation and thermal deactivation
13. Stabilization of proteins by glycosylation examined by NMR analysis of a fucosylated proteinase inhibitor
14. Structural studies of α-N-acetylgalactosaminidase: Effect of glycosylation on the level of expression, secretion efficiency, and enzyme activity
15. Recent advances in the expression of foreign genes in Pichia pastoris
16. Strategies for optimizing protein expression and secretion in the methylotrophic yeast Pichia pastoris
17. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter
18. Factors enhancing genetic transformation of intact yeast cells modify cell wall porosity
19. High-level production of recombinant chicken cystatin by Pichia pastoris and its application in mackerel surimi
20. Cleavage of structural proteins during the assembly of the head bacteriophage T4
21. Improved staining of proteins in polyacrylaminde gel including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie brilliant blue G-250 and R-250
22. Influence of wound injury on accumulation of proteinase inhibitors in leaf and stem tissues of two processing tomato cultivars
23. Cathepsin B cathepsins H, and cathepsins L.
24. i
25. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal of protein-dye binding
26. Recombinant chicken egg white cystatin variants of the QLVSG region
27. Recombinant Q53E- and Q53N-chicken egg white cystatin variants inhibit papain, actinidin and cathepsins B
28. Studies on the control of denaturation of fish muscle proteins during frozen storage
29. The role of N-linked oligosaccharides in glycoproteins function
30. Hyperglycosylation of hen egg white lysozyme in yeast
31. Enhanced secretion of hydrophobic peptide fused lysozyme by the introduction of N-glycosylation signal and the disruption of calnexin gene in Saccharomyces cerevisiae
32. Site-selective glycosylation of subtilisin Bacillus lentus causes dramatic increases in esterase activity