High-level expression, secretion, and purification of the thermostable aqualysin I from Thermus aquaticus YT-1 in Pichia pastoris

Protein Expression and Purification

Volumn 29, Issue 2, 2003, Pages 223-229

  Olȩdzka, Gabriela ^a   Da̧browski, Sławomir ^a   Kur, Józef ^a  

^a GDANSK UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

Aqualysin I; Pichia pastoris; Protein secretion; Serine protease; Thermostable enzymes; Thermus aquaticus YT 1

Indexed keywords

PICHIA; PICHIA PASTORIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; THERMUS; THERMUS AQUATICUS;

EID: 0037791002     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(03)00060-3     Document Type: Article

References (18)

1. H. Matsuzawa, M. Hamaoki, T. Ohta, Production of thermophilic extracellular proteases (aqualysins I and II) by Thermus aquaticus YT-1, an extreme thermophile, Agri. Biol. Chem. 47 (1983) 25-28.
2. H. Matsuzawa, K. Tokugawa, M. Hamaoki, H. Taguchi, I. Terada, S.-T. Kwon, T. Ohta, Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1, Eur. J. Biochem. 171 (1988) 441-447.
3. S.-T. Kwon, I. Terada, H. Matsuzawa, T. Ohta, Nucleotide sequence of the gene for aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1 and characteristics of the deduced primary structure of the enzyme, Eur. J. Biochem. 173 (1988) 491-497.
4. 2- and COOH-terminal prosequences and its processing in Escherichia coli, J. Biol. Chem. 265 (1990) 6576-6581.
5. 2-terminal pro-sequence in the production of active aqualysin I (a thermophilic serine protease) in Escherichia coli, Agri. Biol. Chem. 55 (1991) 3027-3032.
6. Y.-C. Lee, H. Koike, H. Taguchi, T. Ohta, H. Matsuzawa, Requirement of a COOH-terminal pro-sequence for the extracellular secretion of aqualysin I (a thermophilic subtilisin-type protease) in Thermus thermophilus, FEMS Microbiol. Lett. 120 (1994) 69-74.
7. D.-W. Kim, Y.-C. Lee, H. Matsuzawa, Role of the COOH-terminal pro-sequence of aqualysin I (a heat-stable serine protease) in its extracellular secretion by Thermus thermophilus, FEMS Microbiol. Lett. 157 (1997) 39-45.
8. K. Kurosaka, T. Ohta, H. Matsuzawa, A 38 kDa precursor protein of aqualysin I (a thermophilic subtilisin-type protease) with a C-terminal extended sequence: Its purification and in vitro processing, Mol. Microbiol. 20 (1996) 385-389.
9. H. Takagi, M. Koga, S. Katsurada, Y. Yabuta, U. Shinde, M. Inouye, S. Nakamori, Functional analysis of the propeptides of subtilisin E and aqualysin I as intramolecular chaperones, FEBS Lett. 16 (2001) 210-214.
10. J.F. Tschopp, G. Sverlow, R. Kosson, W. Craig, L. Grinna, High-level secretion of glycosylated invertase in the methylotrophic yeast Pichia pastoris, Biotechnology 5 (1987) 1305-1308.
11. J.J. Clare, F.B. Rayment, S.P. Ballantine, K. Sreekrishna, M.A. Romanos, High-level expression of tetanus toxin fragment C in Pichia pastoris strain containing multiple tandem integrations of the gene, Bio/technology 9 (1991) 455-460.
12. E. Paifer, E. Margolles, J. Cremata, R. Montesino, L. Herrera, J.M. Delgado, Efficient expression and secretion of recombinant alpha amylase in Pichia pastoris using two different signal sequences, Yeast 10 (1994) 1415-1419.
13. H.R. Waterham, M.E. Digan, P.J. Koutz, S.V. Lair, J.M. Cregg, Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter, Gene 186 (1997) 37-44.
14. S. Da̧browski, J. Kur, Cloning, overexpression, and purification of the recombinant His-tagged SSB protein of Escherichia coli and use in polymerase chain reaction amplification, Protein Expr. Purif. 16 (1999) 96-102.
15. S. Da̧browski, J. Kur, Cloning and expression in Escherichia coli of the recombinant His-tagged DNA polymerases from Pyrococcus furiosus and Pyrococcus woesei, Protein Expr. Purif. 14 (1998) 131-138.
16. S. Linder, M. Schliwa, S. Kube-Granerath, Direct PCR screening of Pichia pastoris clones, BioTechnique 20 (1996) 980-982.
17. M. Kunitz, J. Gen. Physiol. 30 (1946/47) 291.
18. U.K. Laemmli, Cleavage of structural protein during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.