Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris

Biotechnology Letters

Volumn 27, Issue 5, 2005, Pages 339-345

  Mansur, Manuel ^a   Cabello, Cecilia ^a   Hernández, Lester ^a   País, José ^a   Varas, Laura ^a   Valdés, Jorge ^a   Terrero, Yanet ^a   Hidalgo, Abdel ^a   Plana, Liuba ^b   Besada, Vladimir ^a   García, Liudys ^a   Lamazares, Emilio ^a   Castellanos, Lila ^a   Martínez, Eduardo ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

^b Natl Inst Fundamental Res T   (Cuba)

Author keywords

Insulin; Multiple gene copies; Pichia pastoris; Protein expression

Indexed keywords

ALCOHOLS; GENES; MASS SPECTROMETRY; PROTEINS; YEAST;

INSULIN PRECURSOR SECRETION; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE; SYNTHETIC GENES;

INSULIN;

ALCOHOL DEHYDROGENASE; ALCOHOL OXIDASE; DISULFIDE; GLUTAMYL ENDOPEPTIDASE; PEPTIDE; PROINSULIN; SERINE PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; BIOTECHNOLOGY; CHEMISTRY; FERMENTATION; GENE DOSAGE; GENE VECTOR; GENETIC PROCEDURES; GENETICS; HUMAN; KINETICS; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; PEPTIDE MAPPING; PICHIA; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; SECRETION; TIME;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; BIOTECHNOLOGY; CLONING, MOLECULAR; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; GENE DOSAGE; GENETIC TECHNIQUES; GENETIC VECTORS; HUMANS; KINETICS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PEPTIDES; PICHIA; PLASMIDS; PROINSULIN; PROMOTER REGIONS (GENETICS); SACCHAROMYCES CEREVISIAE; SERINE ENDOPEPTIDASES; TIME FACTORS;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 20244370528     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10529-005-1007-7     Document Type: Article

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