Enhancement of the production of SAM by overexpression of SAM synthetase in Pichia pastoris

Acta Biochimica et Biophysica Sinica

Volumn 35, Issue 2, 2003, Pages 127-132

  Yu, Zhi Liang ^c   Wu, Xing Jia ^a   Li, Dong Yang ^a   Yang, Sheng ^b   Zhou, Zheng ^a   Cai, Jin ^c   Yuan, Zhong Yi ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c ZHEJIANG UNIVERSITY   (China)

Author keywords

Expression optimization; Metabolic engineering; P. pastoris; S Adenosyl L methionine; SAM synthetase 2

Indexed keywords

METHIONINE ADENOSYLTRANSFERASE; S ADENOSYLMETHIONINE;

ARTICLE; CHEMICAL REACTION; FERMENTATION; FUNGAL STRAIN; GENE EXPRESSION; HETEROLOGOUS EXPRESSION; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

BLEOMYCIN; CELL DIVISION; DRUG RESISTANCE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, FUNGAL; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HYDROGEN-ION CONCENTRATION; METHIONINE; METHIONINE ADENOSYLTRANSFERASE; PICHIA; PLASMIDS; PROMOTER REGIONS (GENETICS); S-ADENOSYLMETHIONINE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TIME FACTORS; TRANSFORMATION, GENETIC;

PICHIA; PICHIA PASTORIS; VECTORS;

EID: 0037261058     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Lu SC. S-Aenosylmethionine. Int J Biochem Cell Biol, 2000, 32: 391-395
2. Matos JR, Raushel FM, Wong CH. S-Adenosylmethionine: Studies on chemical and enzymatic synthesis. Biotechnol Appl Biochem, 1987, 9(1): 39-52
3. Reed BR, Patterson II HD. Enzymatic synthesis of S-adenosylmethionine. 1986-07-30, EP0189322
4. Shiozaki S, Shimizu S, Yamada H. Unusual intracellular accumulation of S-adenosyl-L-methionine by microorganisms. Agric Biol Chem, 1984, 48(9): 2293-2300
5. Thomas D, Surdin-Kerjan Y. The synthesis of the two S-adenosyl-methionine synthetases is differently regulated in Saccharomyces cerevisiae. Mol Gen Genet, 1991, 226(1-2): 224-232
6. Park J, Tai J, Roessner CA, Scott AI. Enzymatic synthesis of S-adenosyl-L-methionine on the preparative scale. Bioorg Med Chem, 1996, 4(12): 2179-2185
7. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Simth JA, Struhl K. Short Protocols in Molecalar Biology, 2nd ed. New York: John Wiley & Sons, 1995
8. Li DY, Yu J, Tian L, Ji XS, Yuan ZY. Production of SAM by recombinant Pichia pastoris. Chin J Biotechnol, 2002, 18(3): 295-299
9. Waterham HR, Digan ME, Koutz PJ, Lair SV, Cregg JM. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene, 1997, 186: 37-44
10. Sears IB, O'Connor J, Rossanese OW, Glick BS. A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris. Yeast, 1998, 14: 783-790
11. Shiozaki S, Shimizu S, Yamada H. Production of S-adenosyl-L-methionine by Saccharomyces sake. J Biotechnol, 1986, 4(6): 345-354
12. Shiozaki S, Yamada H, Tani Y, Shimizu S. Yeast culture containing S-adenosylmethionine in high concentrations, and process for production of S-adenosylmethionine. 1985-12-31, US4562149
13. Shiozaki S, Shimizu S, Yamada H. S-Adenosyl-L-methionine production by Saccharomyces sake: Optimization of the culture conditions for the production of cells with a high S-Adenosyl-L-methionine content. Agric Biol Chem, 1989, 53(12): 3269-3274
14. Romanos MA, Scorer CA, Clare JJ. Foreign gene expression in yeast: A review. Yeast, 1992, 8: 423-488