Engineering thermal stability of l-asparaginase by in vitro directed evolution

FEBS Journal

Volumn 276, Issue 6, 2009, Pages 1750-1761

  Kotzia, Georgia A ^a   Labrou, Nikolaos E ^a  

^a AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

Author keywords

Directed evolution; Enzyme engineering; Leukaemia; Saturation mutagenesis; Thermal stability

Indexed keywords

AMMONIA; ASPARAGINASE; ASPARAGINE; LEUCINE;

ACUTE LYMPHOBLASTIC LEUKEMIA; AMINO ACID SEQUENCE; ARTICLE; ENZYME ENGINEERING; GENETIC SCREENING; HYDROLYSIS; IN VITRO DIRECTED EVOLUTION; IN VITRO STUDY; MOLECULAR EVOLUTION; NONHUMAN; PECTOBACTERIUM CAROTOVORUM; PECTOBACTERIUM CHRYSANTHEMI; POINT MUTATION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SITE SATURATION MUTAGENESIS; THERMOSTABILITY;

ASPARAGINASE; BASE SEQUENCE; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; HOT TEMPERATURE; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN ENGINEERING; STATIC ELECTRICITY;

ERWINIA CHRYSANTHEMI; PECTOBACTERIUM CAROTOVORUM;

EID: 61349130050     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2009.06910.x     Document Type: Article

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