A nonradioactive high-throughput assay for screening and characterization of adenylation domains for nonribosomal peptide combinatorial biosynthesis

Analytical Biochemistry

Volumn 386, Issue 2, 2009, Pages 244-250

  McQuade, Thomas J ^a   Shallop, Abbie D ^b   Sheoran, Anita ^b   DelProposto, James E ^a   Tsodikov, Oleg V ^b   Garneau Tsodikova, Sylvie ^b  

^a Life Sciences Institute   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Adenylation domain; Colorimetric; Combinatorial biosynthesis; High throughput screening; Inorganic pyrophosphatase; Malachite green; Nonradioactive; Nostocyclopeptide

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; CARBONATE MINERALS; CONTROLLED DRUG DELIVERY; ENZYMES; PEPTIDES; TARGETED DRUG DELIVERY; THROUGHPUT;

ADENYLATION DOMAINS; COLORIMETRIC; COMBINATORIAL BIOSYNTHESIS; HIGH THROUGHPUT SCREENING; INORGANIC PYROPHOSPHATASES; MALACHITE GREEN; NONRADIOACTIVE; NOSTOCYCLOPEPTIDE;

BIOCHEMISTRY;

ADENOSINE PHOSPHATE; ADENOSINE TRIPHOSPHATE DERIVATIVE; AMINO ACID; INORGANIC PYROPHOSPHATASE; MALACHITE GREEN; NCPB A4 PROTEIN; PEPTIDE; PHOSPHATE; PROTEIN; PYROPHOSPHATE; PYROPHOSPHORIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ADENYLATION; ARTICLE; ASSAY; BIOSYNTHESIS; CATALYSIS; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; ENZYME DEGRADATION; KINETICS; NONHUMAN; NONRIBOSOMAL PEPTIDE SYNTHESIS; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; QUANTITATIVE ANALYSIS; SCREENING;

EID: 59749101384     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.12.014     Document Type: Article

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