Development of a highthroughput yeast-based assay for detection of metabolically activated genotoxins

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 653, Issue 1-2, 2008, Pages 63-69

  Liu, Xuemei ^a   Kramer, Jeffrey A ^a   Swaffield, Jonathan C ^a   Hu, Yi ^a   Chai, Guixuan ^a   Wilson, Alan G E ^a  

^a LEXICON PHARMACEUTICALS INC   (United States)

Author keywords

Dual luciferase; Genetic toxicology; Highthroughput; Metabolic activation; Mutagens; Yeast

Indexed keywords

CLASTOGEN; DNA; FIREFLY LUCIFERASE; PHOSPHOGLYCERATE KINASE; RAD51 PROTEIN; RECA PROTEIN; RENILLA LUCIFERIN 2 MONOOXYGENASE; S 9; TOXIN; MUTAGENIC AGENT; RECOMBINANT PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN;

AMES TEST; ARTICLE; CELL COUNT; CELL MEMBRANE; CELL WALL; COMPARATIVE STUDY; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; ENZYME ACTIVITY; ENZYME ASSAY; EUKARYOTE; FALSE NEGATIVE RESULT; FIREFLY; FUNGAL STRAIN; GENE; GENETIC RECOMBINATION; GENETIC TRANSFECTION; GENOME; GENOTOXICITY; HIGH THROUGHPUT SCREENING; HOMOLOGOUS RECOMBINATION; METABOLIC ACTIVATION; MUTAGENICITY; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; SAMPLE; TIME; YEAST; YEAST CELL; ANIMAL; BIOSYNTHESIS; DRUG EFFECT; GENETICS; METABOLISM; METHODOLOGY; MUTAGEN TESTING; PROMOTER REGION; RENILLA; SACCHAROMYCES CEREVISIAE; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); EUKARYOTA; RENILLA LUCIFERASE; SALMONELLA;

ANIMALS; DNA REPAIR; GENOME, FUNGAL; LUCIFERASES, RENILLA; MUTAGENICITY TESTS; MUTAGENS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; RENILLA; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SENSITIVITY AND SPECIFICITY;

EID: 49649129964     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2008.03.006     Document Type: Article

References (66)

1. Lorge E., Gervais V., Becourt-Lhote N., Maisonneuve C., Delongeas J.L., and Claude N. Genetic toxicity assessment: employing the best science for human safety evaluation. Part IV. A strategy in genotoxicity testing in drug development: some examples. Toxicol. Sci. 98 (2007) 39-42
2. Thybaud V., Aardema M., Clements J., Dearfield K., Galloway S., Hayashi M., Jacobson-Kram D., Kirkland D., MacGregor J.T., Marzin D., Ohyama W., Schuler M., Suzuki H., and Zeiger E. Strategy for genotoxicity testing: hazard identification and risk assessment in relation to in vitro testing. Mutat. Res. 627 (2007) 41-58
3. Muller L., Kikuchi Y., Probst G., Schechtman L., Shimada H., Sofuni T., and Tweats D. ICH-harmonised guidances on genotoxicity testing of pharmaceuticals: evolution, reasoning and impact. Mutat. Res. 436 (1999) 195-225
4. Fluckiger-Isler S., Baumeister M., Braun K., Gervais V., Hasler-Nguyen N., Reimann R., Van Gompel J., Wunderlich H.G., and Engelhardt G. Assessment of the performance of the Ames II assay: a collaborative study with 19 coded compounds. Mutat. Res. 558 (2004) 181-197
5. Ptitsyn L.R., Horneck G., Komova O., Kozubek S., Krasavin E.A., Bonev M., and Rettberg P. A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells. Appl. Environ. Microbiol. 63 (1997) 4377-4384
6. Quillardet P., Huisman O., D'Ari R., and Hofnung M. SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity. Proc. Natl. Acad. Sci. U.S.A. 79 (1982) 5971-5975
7. Chen C., and Kolodner R.D. Gross chromosomal rearrangements in Saccharomyces cerevisiae replication and recombination defective mutants. Nat. Genet. 23 (1999) 81-85
8. Vamvakas S., Vock E.H., and Lutz W.K. On the role of DNA double-strand breaks in toxicity and carcinogenesis. Crit. Rev. Toxicol. 27 (1997) 155-174
9. Khanna K.K., and Jackson S.P. DNA double-strand breaks: signaling, repair and the cancer connection. Nat. Genet. 27 (2001) 247-254
10. Kirpnick Z., Homiski M., Rubitski E., Repnevskaya M., Howlett N., Aubrecht J., and Schiestl R.H. Yeast DEL assay detects clastogens. Mutat. Res. 582 (2005) 116-134
11. Forsburg S.L. The art and design of genetic screens: yeast. Nat. Rev. Genet. 2 (2001) 659-668
12. Billinton N., Barker M.G., Michel C.E., Knight A.W., Heyer W.D., Goddard N.J., Fielden P.R., and Walmsley R.M. Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor. Biosens. Bioelectron. 13 (1998) 831-838
13. Knight A.W., Billinton N., Cahill P.A., Scott A., Harvey J.S., Roberts K.J., Tweats D.J., Keenan P.O., and Walmsley R.M. An analysis of results from 305 compounds tested with the yeast RAD54-GFP genotoxicity assay (GreenScreen GC) including relative predictivity of regulatory tests and rodent carcinogenesis and performance with autofluorescent and coloured compounds. Mutagenesis (2007)
14. Knight A.W., Keenan P.O., Goddard N.J., Fielden P.R., and Walmsley R.M. A yeast-based cytotoxicity and genotoxicity assay for environmental monitoring using novel portable instrumentation. J. Environ. Monit. 6 (2004) 71-79
15. Walmsley R.M., Billinton N., and Heyer W.D. Green fluorescent protein as a reporter for the DNA damage-induced gene RAD54 in Saccharomyces cerevisiae. Yeast 13 (1997) 1535-1545
16. Krogh B.O., and Symington L.S. Recombination proteins in yeast. Annu. Rev. Genet. 38 (2004) 233-271
17. Ivanov E.L., and Haber J.E. DNA repair: RAD alert. Curr. Biol. 7 (1997) R492-R495
18. Billinton N., and Knight A.W. Seeing the wood through the trees: a review of techniques for distinguishing green fluorescent protein from endogenous autofluorescence. Anal. Biochem. 291 (2001) 175-197
19. McNabb D.S., Reed R., and Marciniak R.A. Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryot. Cell 4 (2005) 1539-1549
20. Clever B., Interthal H., Schmuckli-Maurer J., King J., Sigrist M., and Heyer W.D. Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins. Embo J. 16 (1997) 2535-2544
21. Resnick M.A., and Martin P. The repair of double-strand breaks in the nuclear DNA of Saccharomyces cerevisiae and its genetic control. Mol. Gen. Genet. 143 (1976) 119-129
22. Shinohara A., Gasior S., Ogawa T., Kleckner N., and Bishop D.K. Saccharomyces cerevisiae recA homologues RAD51 and DMC1 have both distinct and overlapping roles in meiotic recombination. Genes Cells 2 (1997) 615-629
23. Keller-Seitz M.U., Certa U., Sengstag C., Wurgler F.E., Sun M., and Fasullo M. Transcriptional response of yeast to aflatoxin B1: recombinational repair involving RAD51 and RAD1. Mol. Biol. Cell 15 (2004) 4321-4336
24. U.S. Government Printing Office. NIH Guidelines for the Laboratory Use of Chemical Carcinogens (1981), U.S. Government Printing Office, Washington, DC
25. Myers A.M., Tzagoloff A., Kinney D.M., and Lusty C.J. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45 (1986) 299-310
26. Gietz R.D., and Woods R.A. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol. 350 (2002) 87-96
27. Sherman F. Getting started with yeast. Methods Enzymol. 350 (2002) 3-41
28. Mortelmans K., and Zeiger E. The Ames Salmonella/microsome mutagenicity assay. Mutat. Res. 455 (2000) 29-60
29. Cahill P.A., Knight A.W., Billinton N., Barker M.G., Walsh L., Keenan P.O., Williams C.V., Tweats D.J., and Walmsley R.M. The GreenScreen genotoxicity assay: a screening validation programme. Mutagenesis 19 (2004) 105-119
30. Chang M., Bellaoui M., Boone C., and Brown G.W. A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 16934-16939
31. Lupardus P.J., Byun T., Yee M.C., Hekmat-Nejad M., and Cimprich K.A. A requirement for replication in activation of the ATR-dependent DNA damage checkpoint. Genes Dev. 16 (2002) 2327-2332
32. Kirkland D., Aardema M., Henderson L., and Muller L. Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens. Part I. Sensitivity, specificity and relative predictivity. Mutat. Res. 584 (2005) 1-256
33. Kirkland D., Aardema M., Muller L., and Makoto H. Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens. Part II. Further analysis of mammalian cell results, relative predictivity and tumour profiles. Mutat. Res. 608 (2006) 29-42
34. Perkins E.L., Sterling J.F., Hashem V.I., and Resnick M.A. Yeast and human genes that affect the Escherichia coli SOS response. Proc. Natl. Acad. Sci. U.S.A. 96 (1999) 2204-2209
35. Bressan D.A., Baxter B.K., and Petrini J.H. The Mre11-Rad50-Xrs2 protein complex facilitates homologous recombination-based double-strand break repair in Saccharomyces cerevisiae. Mol. Cell Biol. 19 (1999) 7681-7687
36. Arai R., Makita Y., Oda Y., and Nagamune T. Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/umu-test) and improvement of mutagen-sensitivity. J. Biosci. Bioeng. 92 (2001) 301-304
37. Mitchell R.J., and Gu M.B. An Escherichia coli biosensor capable of detecting both genotoxic and oxidative damage. Appl. Microbiol. Biotechnol. 64 (2004) 46-52
38. Jackson S.P. Colworth Medal lecture. Detection, repair and signalling of DNA double-strand breaks. Biochem. Soc. Trans. 27 (1999) 1-13
39. Thacker J. The nature of mutants induced by ionising radiation in cultured hamster cells. Part III. Molecular characterization of HPRT-deficient mutants induced by gamma-rays or alpha-particles showing that the majority have deletions of all or part of the hprt gene. Mutat. Res. 160 (1986) 267-275
40. Jeggo P.A. Identification of genes involved in repair of DNA double-strand breaks in mammalian cells. Radiat. Res. 150 (1998) S80-S91
41. Kanaar R., Hoeijmakers J.H., and van Gent D.C. Molecular mechanisms of DNA double strand break repair. Trends Cell. Biol. 8 (1998) 483-489
42. New J.H., Sugiyama T., Zaitseva E., and Kowalczykowski S.C. Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A. Nature 391 (1998) 407-410
43. Dudasova Z., Dudas A., Alemayehu A., Vlasakova D., Markova E., Chovanec M., Vlckova V., and Brozmanova J. Disruption of the RAD51 gene sensitizes S. cerevisiae cells to the toxic and mutagenic effects of hydrogen peroxide. Folia Microbiol. (Praha) 49 (2004) 259-264
44. Jensen L.H., Dejligbjerg M., Hansen L.T., Grauslund M., Jensen P.B., and Sehested M. Characterisation of cytotoxicity and DNA damage induced by the topoisomerase II-directed bisdioxopiperazine anti-cancer agent ICRF-187 (dexrazoxane) in yeast and mammalian cells. BMC Pharmacol. 4 (2004) 31
45. Iversen O.H. Urethan (ethyl carbamate) alone is carcinogenic for mouse skin. Carcinogenesis 5 (1984) 911-915
46. Spagnuolo G., Mauro C., Leonardi A., Santillo M., Paterno R., Schweikl H., Avvedimento E.V., and Rengo S. NF-kappaB protection against apoptosis induced by HEMA. J. Dent. Res. 83 (2004) 837-842
47. Sato S., Taketomi M., and Morita T. Simplified mouse peripheral reticulocyte micronucleus test with dimethylnitrosamine. Mutat. Res. 278 (1992) 103-107
48. Jagetia G.C., and Jyothi P. Enhancement of micronuclei frequency by vindesine in mouse bone marrow. Mutat. Res. 388 (1997) 1-5
49. Tinwell H., Lefevre P.A., and Ashby J. Relative activities of methyl methanesulphonate (MMS) as a genotoxin, clastogen and gene mutagen to the liver and bone marrow of MutaMouse mice. Environ. Mol. Mutagen. 32 (1998) 163-172
50. Valentin-Severin I., Thybaud V., Le Bon A.M., Lhuguenot J.C., and Chagnon M.C. The autoradiographic test for unscheduled DNA synthesis: a sensitive assay for the detection of DNA repair in the HepG2 cell line. Mutat. Res. 559 (2004) 211-217
51. Hooker A.M., Horne R., Morley A.A., and Sykes P.J. Dose-dependent increase or decrease of somatic intrachromosomal recombination produced by etoposide. Mutat. Res. 500 (2002) 117-124
52. Gavin I.M., Melnik S.M., Yurina N.P., Khabarova M.I., and Bavykin S.G. Zero-length protein-nucleic acid crosslinking by radical-generating coordination complexes as a probe for analysis of protein-DNA interactions in vitro and in vivo. Anal. Biochem. 263 (1998) 26-30
53. Bond J.A., and Li A.P. Rat nasal tissue activation of benzo(a)pyrene and 2-aminoanthracene to mutagens in Salmonella typhimurium. Environ. Mutagen. 5 (1983) 311-318
54. Witt K.L., and Bishop J.B. Mutagenicity of anticancer drugs in mammalian germ cells. Mutat. Res. 355 (1996) 209-234
55. Galloway S.M., Miller J.E., Armstrong M.J., Bean C.L., Skopek T.R., and Nichols W.W. DNA synthesis inhibition as an indirect mechanism of chromosome aberrations: comparison of DNA-reactive and non-DNA-reactive clastogens. Mutat. Res. 400 (1998) 169-186
56. Olsen O., Wang X., von D., and Wettstein. Sodium azide mutagenesis: preferential generation of A.T-->G.C transitions in the barley Ant18 gene. Proc. Natl. Acad. Sci U.S.A. 90 (1993) 8043-8047
57. Lee G.S., Blonsky K.S., Van On D.L., Savage E.A., Morgan A.R., von R.C., and Borstel. Base alterations in yeast induced by alkylating agents with differing Swain-Scott substrate constants. J. Mol. Biol. 223 (1992) 617-626
58. Burnouf D., Duane M., and Fuchs R.P. Spectrum of cisplatin-induced mutations in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 84 (1987) 3758-3762
59. Hartwig A., Groblinghoff U.D., Beyersmann D., Natarajan A.T., Filon R., and Mullenders L.H. Interaction of arsenic(III) with nucleotide excision repair in UV-irradiated human fibroblasts. Carcinogenesis 18 (1997) 399-405
60. Kang J.M., Iovine N.M., and Blaser M.J. A paradigm for direct stress-induced mutation in prokaryotes. FASEB J. 20 (2006) 2476-2485
61. Ambler J.E., and Pinney R.J. Positive R plasmid mutator effect on chromosomal mutation to nalidixic acid resistance in nalidixic acid-exposed cultures of Escherichia coli. J. Antimicrob. Chemother. 35 (1995) 603-609
62. Cui X.S., Eriksson L.C., and Moller L. Formation and persistence of DNA adducts during and after a long-term administration of 2-nitrofluorene. Mutat. Res. 442 (1999) 9-18
63. Mailhes J.B., Carabatsos M.J., Young D., London S.N., Bell M., and Albertini D.F. Taxol-induced meiotic maturation delay, spindle defects, and aneuploidy in mouse oocytes and zygotes. Mutat. Res. 423 (1999) 79-90
64. Benedict W.F., and Karon M. Chromatid breakage: cytosine arabinoside-induced lesions inhibited by ultraviolet irradiation. Science 171 (1971) 680-682
65. Kundu G.C., Schullek J.R., and Wilson I.B. The alkylating properties of chlorambucil. Pharmacol. Biochem. Behav. 49 (1994) 621-624
66. Pincus S., and Rekosh D. The inhibition of both initiation and elongation of adenovirus DNA replication by actinomycin D. Biochem. Biophys. Res. Commun. 121 (1984) 1033-1041