A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells

Applied and Environmental Microbiology

Volumn 63, Issue 11, 1997, Pages 4377-4384

  Ptitsyn, L R ^a   Horneck, G ^b   Komova, O ^c   Kozubek, S ^d   Krasavin, E A ^c   Bonev, M ^e   Rettberg, P ^b  

^a STATE RESEARCH INSTITUTE OF GENETICS AND SELECTION OF INDUSTRIAL MICROORGANISMS   (Russian Federation)

^b GERMAN AEROSPACE CENTER DLR   (Germany)

^c JOINT INSTITUTE FOR NUCLEAR RESEARCH   (Russian Federation)

^d INSTITUTE OF MATHEMATICS   (Czech Republic)

^e INSTITUTE FOR NUCLEAR RESEARCH AND NUCLEAR ENERGY   (Bulgaria)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BIOASSAY; BIOSENSOR; ESCHERICHIA COLI; GENOTOXICITY; MUTAGENICITY; NONHUMAN; POLLUTION; TOXIN ANALYSIS;

EID: 0030712223     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.11.4377-4384.1997     Document Type: Article

References (38)

1. Ames, B. N. 1979. Identifying environmental chemicals causing mutations and cancer. Science 204:587-593.
2. Ames, B. N., J. McCann, and E. Yamasaki. 1975. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mutat. Res. 31:347-364.
3. Bachmann, B. J. 1972. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol. Rev. 36:525-577.
4. Burlage, R. S., G. S. Sayler, and F. Larimer. 1990. Monitoring of naphthalene catabolism by bioluminescence with nah-lux transcriptional fusions. J. Bacteriol. 172:4749-4757.
5. Chatterjee, J., and E. A. Meighen. 1995. Biotechnical applications of bacterial bioluminescence (lux) genes. Photochem. Photobiol. 62:641-650.
6. Corbisier, P., G. Ji, G. Nuyts, M. Mergeay, and S. Silver. 1993. luxAB gene fusions with the arsenic and cadmium resistance operons of Staphylococcus aureus plasmid pI258. FEMS Microbiol. Lett. 110:2231-2238.
7. Frey, J., P. Ghersa, P. G. Palacios, and M. Belet. 1986. Physical and genetic analysis of the ColD plasmid. J. Bacteriol. 166:15-19.
8. Heitzer, A., K. Malachowski, J. E. Thonnard, P. R. Bienkowski, D. C. White, and G. S. Sayler. 1994. Optical biosensor for environmental on-line monitoring of naphthalene and salicylate bioavailability with an immobilized bioluminescent catabolic reporter bacterium. Appl. Environ. Microbiol. 60: 1487-1494.
9. Isildar, M., and G. Bakale. 1984. Radiation-induced mutagenicity and lethality in Ames tester strains of Salmonella. Radiat. Res. 100:396-411.
10. Koudela, K., L. Ryznar, S. Kozubek, and J. Slotova. 1992. Induction of SOS repair by ionizing radiation. Radiat. Environ. Biophys. 31:343-348.
11. Kozubek, S., E. A. Krasavin, J. Soska, V. Drásil, K. G. Amiratyev, B. Tokarova, and M. Bonev. 1989. Induction of the SOS response in Escherichia coli by heavy ions. Mutat. Res. 215:49-53.
12. Kozubek, S., E. A. Krasavin, K. G. Amiratyev, B. Tokarova, J. Soska, V. Drásil, and M. Bonev. 1989. The induction of revertants by heavy particles and γ-rays in Salmonella tester strains. Mutat. Res. 210:221-226.
13. Kozubek, S., M. M. Ogievetskaya, E. A. Krasavin, V. Drásil, and J. Soska. 1990. Investigation of the SOS response of Escherichia coli after γ-irradiation by means of the SOS chromotest. Mutat. Res. 230:1-7.
14. Lee, C. Y., R. B. Szittner, and E. A. Meighen. 1991. The lux genes of luminous bacterial symbiont Photobacterium leiognathi of the ponyfish. Eur. J. Biochem. 201:161-167.
15. Levin, D. E., E. Yamasaki, and B. N. Ames. 1982. A new Salmonella tester strain, TA97, for the detection of frameshift mutagens: a run of cytosins as a mutational hot spot. Mutat. Res. 94:315-330.
16. Levin, D. E., M. C. Hollstein, M. F. Christmas, E. A. Schwiers, and B. N. Ames. 1982. A new Salmonella tester strain (TA102) with A:T base pairs at the site of mutation detects oxidative mutagens. Proc. Natl. Acad. Sci. USA 79:7445-7449.
17. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
18. Maron, D. M., and B. N. Ames. 1983. Revised methods for the Salmonella mutagenicity test. Mutat. Res. 113:173-215.
19. McDaniels, A. E., A. L. Reyes, L. J. Wymer, C. C. Rankin, and G. N. Stelma. 1990. Comparison of the Salmonella (Ames) test, umu tests, and the SOS chromotests for detecting genotoxins. Environ. Mol. Mutagen. 16:204-215.
20. Mersch-Sundermann, V., U. Schneider, G. Klopman, and H. S. Rosenkranz. 1994. SOS induction in Escherichia coli and Salmonella mutagenicity: a comparison using 330 compounds. Mutagenesis 9:205-224.
21. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
22. Nakamura, S., Y. Oda, T. Shimida, I. Oki, and K. Sugimoto. 1987. SOS-inducing activity of chemical carcinogens and mutagens in Salmonella typhimurium TA1535/pSK1002: examination with 151 chemicals. Mutat. Res. 192:239-246.
23. Oda, Y., S. Nakamura, I. Oki, T. Kato, and H. Shinagawa. 1985. Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens. Mutat. Res. 147:219-229.
24. Oda, Y., H. Yamazaki, M. Watanabe, T. Nohmi, and T. Shimada. 1995. Development of highly sensitive umu test system: rapid detection of genotoxicity of promutagenic aromatic amines by Salmonella typhimurium strain NM2009 possessing high O-acetyltransferase activity. Mutat. Res. 334:145-156.
25. Ptitsyn, L. R., W. B. Gurevich, T. G. Barsanova, A. N. Shenderov, M. J. Haikinson, and A. I. Stepanov. 1988. The cloning and insertional mutagenesis of a DNA fragment coding the bioluminescence system of Photobacterium leiognathi. Mol. Genet. Mikrobiol. Virusol. 14:17-19. (In Russian.)
26. Ptitsyn, L. R., M. A. Fatova, and A. I. Stepanov. 1990. Expression of the bioluminescence system of Photobacterium leiognathi in E. coli. Mol. Genet. Mikrobiol. Virusol. 16:26-29. (In Russian.)
27. Quillardet P., O. Huisman, R. D. Ari, and M. Hofnung. 1982. SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K12 to measure genotoxicity. Proc. Natl. Acad. Sci. USA 79:5971-5975.
28. Quillardet P., and M. Hofnung. 1984. Induction by UV light of the SOS function sfiA in Escherichia coli strains deficient or proficient in excision repair. J. Bacteriol. 157:35-38.
29. Quillardet, P., G. Frelat, V. D. Nguyen, and M. Hofnung. 1989. Detection of ionizing radiation with the SOS chromotest, a bacterial short-term test for genotoxic agents. Mutat. Res. 216:251-257.
30. Quillardet, P., and M. Hofnung. 1993. The SOS chromotest: a review. Mutat. Res. 297:235-279.
31. Radman, M. 1975. Phenomenology of an inducible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis, p. 128-142. In L. Prakash, F. Sherman, M. Miller, C. Laurence, and H. W. Taylor (ed.), Molecular and environmental aspects of mutagenesis. Thomas, Springfield, Ill.
32. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
33. Scheller, F., and R. D. Schmid. 1991. Biosensors: fundamentals, technologies and applications. VCH Verlagsgesellschaft, Weinheim, Germany.
34. Selfinova, O., R. Burlage, and T. Barkay. 1993. Bioluminescent sensors for detection of bioavailable Hg(II) in the environment. Appl. Environ. Microbiol. 59:3083-3090.
35. Stewart, G. S. A. B., S. P. Denyer, and J. Lewington. 1991. Microbiology illuminated: gene engineering and bioluminescence. Trends Food Sci. Technol. Today 2:19-22.
36. Tescione, L., and G. Belfort. 1993. Construction and evaluation of a metal ion biosensor. Biotechnol. Bioeng. 42:945-952.
37. White, P. A., and J. B. Rasmussen. 1996. SOS chromotest results in a broader context: empirical relationships between genotoxic potency, mutagenic potency, and carcinogenic potency. Environ. Mol. Mutagen. 27:270-305.
38. Witkin, E. M. 1976. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol. Rev. 40:869-907.