-
1
-
-
37249004920
-
Reaching for high-hanging fruit in drug discovery at protein-protein interfaces
-
Wells J.A., and McClendon C.L. Reaching for high-hanging fruit in drug discovery at protein-protein interfaces. Nature 450 (2007) 1001-1009
-
(2007)
Nature
, vol.450
, pp. 1001-1009
-
-
Wells, J.A.1
McClendon, C.L.2
-
2
-
-
0035987407
-
The post-genomic era of interactive proteomics: facts and perspectives
-
Auerbach D., Thaminy S., Hottiger M.O., and Stagljar I. The post-genomic era of interactive proteomics: facts and perspectives. Proteomics 2 (2002) 611-623
-
(2002)
Proteomics
, vol.2
, pp. 611-623
-
-
Auerbach, D.1
Thaminy, S.2
Hottiger, M.O.3
Stagljar, I.4
-
3
-
-
0024406857
-
A novel genetic system to detect protein-protein interactions
-
Fields S., and Song O. A novel genetic system to detect protein-protein interactions. Nature 340 (1989) 245-246
-
(1989)
Nature
, vol.340
, pp. 245-246
-
-
Fields, S.1
Song, O.2
-
4
-
-
0034628508
-
A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae
-
Uetz P., Giot L., Cagney G., Mansfield T.A., Judson R.S., Knight J.R., Lockshon D., Narayan V., Srinivasan M., Pochart P., et al. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature 403 (2000) 623-627
-
(2000)
Nature
, vol.403
, pp. 623-627
-
-
Uetz, P.1
Giot, L.2
Cagney, G.3
Mansfield, T.A.4
Judson, R.S.5
Knight, J.R.6
Lockshon, D.7
Narayan, V.8
Srinivasan, M.9
Pochart, P.10
-
5
-
-
0035836765
-
A comprehensive two-hybrid analysis to explore the yeast protein interactome
-
Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., and Sakaki Y. A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci U S A 98 (2001) 4569-4574
-
(2001)
Proc Natl Acad Sci U S A
, vol.98
, pp. 4569-4574
-
-
Ito, T.1
Chiba, T.2
Ozawa, R.3
Yoshida, M.4
Hattori, M.5
Sakaki, Y.6
-
6
-
-
38549161592
-
A proteome-wide protein interaction map for Campylobacter jejuni
-
Parrish J.R., Yu J., Liu G., Hines J.A., Chan J.E., Mangiola B.A., Zhang H., Pacifico S., Fotouhi F., DiRita V.J., et al. A proteome-wide protein interaction map for Campylobacter jejuni. Genome Biol 8 (2007) R130
-
(2007)
Genome Biol
, vol.8
-
-
Parrish, J.R.1
Yu, J.2
Liu, G.3
Hines, J.A.4
Chan, J.E.5
Mangiola, B.A.6
Zhang, H.7
Pacifico, S.8
Fotouhi, F.9
DiRita, V.J.10
-
7
-
-
0035843145
-
The protein-protein interaction map of Helicobacter pylori
-
Rain J.C., Selig L., De Reuse H., Battaglia V., Reverdy C., Simon S., Lenzen G., Petel F., Wojcik J., Schachter V., et al. The protein-protein interaction map of Helicobacter pylori. Nature 409 (2001) 211-215
-
(2001)
Nature
, vol.409
, pp. 211-215
-
-
Rain, J.C.1
Selig, L.2
De Reuse, H.3
Battaglia, V.4
Reverdy, C.5
Simon, S.6
Lenzen, G.7
Petel, F.8
Wojcik, J.9
Schachter, V.10
-
8
-
-
0345600247
-
A protein interaction map of Drosophila melanogaster
-
Giot L., Bader J.S., Brouwer C., Chaudhuri A., Kuang B., Li Y., Hao Y.L., Ooi C.E., Godwin B., Vitols E., et al. A protein interaction map of Drosophila melanogaster. Science 302 (2003) 1727-1736
-
(2003)
Science
, vol.302
, pp. 1727-1736
-
-
Giot, L.1
Bader, J.S.2
Brouwer, C.3
Chaudhuri, A.4
Kuang, B.5
Li, Y.6
Hao, Y.L.7
Ooi, C.E.8
Godwin, B.9
Vitols, E.10
-
9
-
-
20144379646
-
Protein interaction mapping: a Drosophila case study
-
Formstecher E., Aresta S., Collura V., Hamburger A., Meil A., Trehin A., Reverdy C., Betin V., Maire S., Brun C., et al. Protein interaction mapping: a Drosophila case study. Genome Res 15 (2005) 376-384
-
(2005)
Genome Res
, vol.15
, pp. 376-384
-
-
Formstecher, E.1
Aresta, S.2
Collura, V.3
Hamburger, A.4
Meil, A.5
Trehin, A.6
Reverdy, C.7
Betin, V.8
Maire, S.9
Brun, C.10
-
10
-
-
27644484749
-
A protein interaction network of the malaria parasite Plasmodium falciparum
-
LaCount D.J., Vignali M., Chettier R., Phansalkar A., Bell R., Hesselberth J.R., Schoenfeld L.W., Ota I., Sahasrabudhe S., Kurschner C., et al. A protein interaction network of the malaria parasite Plasmodium falciparum. Nature 438 (2005) 103-107
-
(2005)
Nature
, vol.438
, pp. 103-107
-
-
LaCount, D.J.1
Vignali, M.2
Chettier, R.3
Phansalkar, A.4
Bell, R.5
Hesselberth, J.R.6
Schoenfeld, L.W.7
Ota, I.8
Sahasrabudhe, S.9
Kurschner, C.10
-
11
-
-
25144498379
-
A human protein-protein interaction network: a resource for annotating the proteome
-
In a systematic analysis of the human interactome, a protein matrix of 4456 baits and 5632 preys was screened and 3186 interactions were identified. Subsequent data analysis and validation revealed 911 high-confidence interactions among 401 proteins.
-
Stelzl U., Worm U., Lalowski M., Haenig C., Brembeck F.H., Goehler H., Stroedicke M., Zenkner M., Schoenherr A., Koeppen S., et al. A human protein-protein interaction network: a resource for annotating the proteome. Cell 122 (2005) 957-968. In a systematic analysis of the human interactome, a protein matrix of 4456 baits and 5632 preys was screened and 3186 interactions were identified. Subsequent data analysis and validation revealed 911 high-confidence interactions among 401 proteins.
-
(2005)
Cell
, vol.122
, pp. 957-968
-
-
Stelzl, U.1
Worm, U.2
Lalowski, M.3
Haenig, C.4
Brembeck, F.H.5
Goehler, H.6
Stroedicke, M.7
Zenkner, M.8
Schoenherr, A.9
Koeppen, S.10
-
12
-
-
27144530248
-
Towards a proteome-scale map of the human protein-protein interaction network
-
Analysis of the human interactome. This study also revealed more than 300 new connections to over 100 disease-associated proteins.
-
Rual J.F., Venkatesan K., Hao T., Hirozane-Kishikawa T., Dricot A., Li N., Berriz G.F., Gibbons F.D., Dreze M., Ayivi-Guedehoussou N., et al. Towards a proteome-scale map of the human protein-protein interaction network. Nature (2005). Analysis of the human interactome. This study also revealed more than 300 new connections to over 100 disease-associated proteins.
-
(2005)
Nature
-
-
Rual, J.F.1
Venkatesan, K.2
Hao, T.3
Hirozane-Kishikawa, T.4
Dricot, A.5
Li, N.6
Berriz, G.F.7
Gibbons, F.D.8
Dreze, M.9
Ayivi-Guedehoussou, N.10
-
13
-
-
33847744247
-
How complete are current yeast and human protein-interaction networks?
-
Hart G.T., Ramani A.K., and Marcotte E.M. How complete are current yeast and human protein-interaction networks?. Genome Biol 7 (2006) 120
-
(2006)
Genome Biol
, vol.7
, pp. 120
-
-
Hart, G.T.1
Ramani, A.K.2
Marcotte, E.M.3
-
14
-
-
39449129810
-
Novel interaction partners of Bardet-Biedl syndrome proteins
-
Oeffner F., Moch C., Neundorf A., Hofmann J., Koch M., and Grzeschik K.H. Novel interaction partners of Bardet-Biedl syndrome proteins. Cell Motil Cytoskeleton 65 (2008) 143-155
-
(2008)
Cell Motil Cytoskeleton
, vol.65
, pp. 143-155
-
-
Oeffner, F.1
Moch, C.2
Neundorf, A.3
Hofmann, J.4
Koch, M.5
Grzeschik, K.H.6
-
15
-
-
34249715853
-
Huntingtin interacting proteins are genetic modifiers of neurodegeneration
-
Kaltenbach L.S., Romero E., Becklin R.R., Chettier R., Bell R., Phansalkar A., Strand A., Torcassi C., Savage J., Hurlburt A., et al. Huntingtin interacting proteins are genetic modifiers of neurodegeneration. PLoS Genet 3 (2007) e82
-
(2007)
PLoS Genet
, vol.3
-
-
Kaltenbach, L.S.1
Romero, E.2
Becklin, R.R.3
Chettier, R.4
Bell, R.5
Phansalkar, A.6
Strand, A.7
Torcassi, C.8
Savage, J.9
Hurlburt, A.10
-
16
-
-
33646687963
-
A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration
-
A comprehensive interaction screening was undertaken for 20 proteins that are involved in inherited ataxias, a class of neurodegenerative disorders with common clinical and pathological features. This work provides an excellent insight into how disease-centered YTH interactome studies can elucidate pathogenic mechanisms and identify candidate genes for complex human diseases.
-
Lim J., Hao T., Shaw C., Patel A.J., Szabo G., Rual J.F., Fisk C.J., Li N., Smolyar A., Hill D.E., et al. A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell 125 (2006) 801-814. A comprehensive interaction screening was undertaken for 20 proteins that are involved in inherited ataxias, a class of neurodegenerative disorders with common clinical and pathological features. This work provides an excellent insight into how disease-centered YTH interactome studies can elucidate pathogenic mechanisms and identify candidate genes for complex human diseases.
-
(2006)
Cell
, vol.125
, pp. 801-814
-
-
Lim, J.1
Hao, T.2
Shaw, C.3
Patel, A.J.4
Szabo, G.5
Rual, J.F.6
Fisk, C.J.7
Li, N.8
Smolyar, A.9
Hill, D.E.10
-
17
-
-
14944383217
-
Automated yeast two-hybrid screening for nuclear receptor-interacting proteins
-
Albers M., Kranz H., Kober I., Kaiser C., Klink M., Suckow J., Kern R., and Koegl M. Automated yeast two-hybrid screening for nuclear receptor-interacting proteins. Mol Cell Proteomics 4 (2005) 205-213
-
(2005)
Mol Cell Proteomics
, vol.4
, pp. 205-213
-
-
Albers, M.1
Kranz, H.2
Kober, I.3
Kaiser, C.4
Klink, M.5
Suckow, J.6
Kern, R.7
Koegl, M.8
-
18
-
-
34948838857
-
A protein interaction map of the mitotic spindle
-
Wong J., Nakajima Y., Westermann S., Shang C., Kang J.S., Goodner C., Houshmand P., Fields S., Chan C.S., Drubin D., et al. A protein interaction map of the mitotic spindle. Mol Biol Cell 18 (2007) 3800-3809
-
(2007)
Mol Biol Cell
, vol.18
, pp. 3800-3809
-
-
Wong, J.1
Nakajima, Y.2
Westermann, S.3
Shang, C.4
Kang, J.S.5
Goodner, C.6
Houshmand, P.7
Fields, S.8
Chan, C.S.9
Drubin, D.10
-
19
-
-
34250170441
-
A yeast two-hybrid smart-pool-array system for protein-interaction mapping
-
Jin F., Avramova L., Huang J., and Hazbun T. A yeast two-hybrid smart-pool-array system for protein-interaction mapping. Nat Methods 4 (2007) 405-407
-
(2007)
Nat Methods
, vol.4
, pp. 405-407
-
-
Jin, F.1
Avramova, L.2
Huang, J.3
Hazbun, T.4
-
20
-
-
0037161731
-
Comparative assessment of large-scale data sets of protein-protein interactions
-
von Mering C., Krause R., Snel B., Cornell M., Oliver S.G., Fields S., and Bork P. Comparative assessment of large-scale data sets of protein-protein interactions. Nature 417 (2002) 399-403
-
(2002)
Nature
, vol.417
, pp. 399-403
-
-
von Mering, C.1
Krause, R.2
Snel, B.3
Cornell, M.4
Oliver, S.G.5
Fields, S.6
Bork, P.7
-
21
-
-
36949000237
-
Where have all the interactions gone? Estimating the coverage of two-hybrid protein interaction maps
-
Huang H., Jedynak B.M., and Bader J.S. Where have all the interactions gone? Estimating the coverage of two-hybrid protein interaction maps. PLoS Comput Biol 3 (2007) e214
-
(2007)
PLoS Comput Biol
, vol.3
-
-
Huang, H.1
Jedynak, B.M.2
Bader, J.S.3
-
22
-
-
0036849482
-
Analysis of membrane protein interactions using yeast-based technologies
-
Stagljar I., and Fields S. Analysis of membrane protein interactions using yeast-based technologies. Trends Biochem Sci 27 (2002) 559-563
-
(2002)
Trends Biochem Sci
, vol.27
, pp. 559-563
-
-
Stagljar, I.1
Fields, S.2
-
23
-
-
0032574840
-
A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo
-
Stagljar I., Korostensky C., Johnsson N., and te Heesen S. A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo. Proc Natl Acad Sci U S A 95 (1998) 5187-5192
-
(1998)
Proc Natl Acad Sci U S A
, vol.95
, pp. 5187-5192
-
-
Stagljar, I.1
Korostensky, C.2
Johnsson, N.3
te Heesen, S.4
-
24
-
-
0028080090
-
Split ubiquitin as a sensor of protein interactions in vivo
-
Johnsson N., and Varshavsky A. Split ubiquitin as a sensor of protein interactions in vivo. Proc Natl Acad Sci U S A 91 (1994) 10340-10344
-
(1994)
Proc Natl Acad Sci U S A
, vol.91
, pp. 10340-10344
-
-
Johnsson, N.1
Varshavsky, A.2
-
25
-
-
0037810425
-
Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system
-
Thaminy S., Auerbach D., Arnoldo A., and Stagljar I. Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system. Genome Res 13 (2003) 1744-1753
-
(2003)
Genome Res
, vol.13
, pp. 1744-1753
-
-
Thaminy, S.1
Auerbach, D.2
Arnoldo, A.3
Stagljar, I.4
-
26
-
-
24744436626
-
Large-scale identification of yeast integral membrane protein interactions
-
Miller J.P., Lo R.S., Ben-Hur A., Desmarais C., Stagljar I., Noble W.S., and Fields S. Large-scale identification of yeast integral membrane protein interactions. Proc Natl Acad Sci U S A 102 (2005) 12123-12128
-
(2005)
Proc Natl Acad Sci U S A
, vol.102
, pp. 12123-12128
-
-
Miller, J.P.1
Lo, R.S.2
Ben-Hur, A.3
Desmarais, C.4
Stagljar, I.5
Noble, W.S.6
Fields, S.7
-
27
-
-
34147167177
-
Mapping protein-protein interactions for the yeast ABC transporter Ycf1p by integrated split-ubiquitin membrane yeast two-hybrid analysis
-
Paumi C.M., Menendez J., Arnoldo A., Engels K., Iyer K.R., Thaminy S., Georgiev O., Barral Y., Michaelis S., and Stagljar I. Mapping protein-protein interactions for the yeast ABC transporter Ycf1p by integrated split-ubiquitin membrane yeast two-hybrid analysis. Mol Cell 26 (2007) 15-25
-
(2007)
Mol Cell
, vol.26
, pp. 15-25
-
-
Paumi, C.M.1
Menendez, J.2
Arnoldo, A.3
Engels, K.4
Iyer, K.R.5
Thaminy, S.6
Georgiev, O.7
Barral, Y.8
Michaelis, S.9
Stagljar, I.10
-
28
-
-
41849128212
-
An optimized split-ubiquitin cDNA-library screening system to identify novel interactors of the human Frizzled 1 receptor
-
Dirnberger D., Messerschmid M., and Baumeister R. An optimized split-ubiquitin cDNA-library screening system to identify novel interactors of the human Frizzled 1 receptor. Nucleic Acids Res 36 (2008) e37
-
(2008)
Nucleic Acids Res
, vol.36
-
-
Dirnberger, D.1
Messerschmid, M.2
Baumeister, R.3
-
29
-
-
47849096465
-
Monitoring protein-protein interactions between the mammalian integral membrane transporters and PDZ-interacting partners using a modified split-ubiquitin membrane yeast two-hybrid system
-
This approach addresses proteins containing PDZ domains that bind to PDZ binding motifs in the C-terminal tails of integral membrane proteins using the MYTH screening system. In this application of the MYTH the transcription factor moiety was shifted to the N-terminus to permit detection of C-terminal PDZ domain interactions.
-
Gisler S.M., Kittanakom S., Fuster D., Wong V., Bertic M., Radanovic T., Hall R.A., Murer H., Biber J., Markovich D., et al. Monitoring protein-protein interactions between the mammalian integral membrane transporters and PDZ-interacting partners using a modified split-ubiquitin membrane yeast two-hybrid system. Mol Cell Proteomics 7 (2008) 1362-1377. This approach addresses proteins containing PDZ domains that bind to PDZ binding motifs in the C-terminal tails of integral membrane proteins using the MYTH screening system. In this application of the MYTH the transcription factor moiety was shifted to the N-terminus to permit detection of C-terminal PDZ domain interactions.
-
(2008)
Mol Cell Proteomics
, vol.7
, pp. 1362-1377
-
-
Gisler, S.M.1
Kittanakom, S.2
Fuster, D.3
Wong, V.4
Bertic, M.5
Radanovic, T.6
Hall, R.A.7
Murer, H.8
Biber, J.9
Markovich, D.10
-
30
-
-
34447130876
-
Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins
-
CytoYTH is a MYTH variant that displays PPIs in the cytoplasm. CytoYTH was applied for transcription factors p53 and members of the NF-kappaB complex. Screening a cDNA library, several new interaction partners were identified for Uri1p, a previously uncharacterized yeast protein.
-
Mockli N., Deplazes A., Hassa P.O., Zhang Z., Peter M., Hottiger M.O., Stagljar I., and Auerbach D. Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins. Biotechniques 42 (2007) 725-730. CytoYTH is a MYTH variant that displays PPIs in the cytoplasm. CytoYTH was applied for transcription factors p53 and members of the NF-kappaB complex. Screening a cDNA library, several new interaction partners were identified for Uri1p, a previously uncharacterized yeast protein.
-
(2007)
Biotechniques
, vol.42
, pp. 725-730
-
-
Mockli, N.1
Deplazes, A.2
Hassa, P.O.3
Zhang, Z.4
Peter, M.5
Hottiger, M.O.6
Stagljar, I.7
Auerbach, D.8
-
31
-
-
0035902450
-
A two-hybrid system for transactivator bait proteins
-
Hirst M., Ho C., Sabourin L., Rudnicki M., Penn L., and Sadowski I. A two-hybrid system for transactivator bait proteins. Proc Natl Acad Sci U S A 98 (2001) 8726-8731
-
(2001)
Proc Natl Acad Sci U S A
, vol.98
, pp. 8726-8731
-
-
Hirst, M.1
Ho, C.2
Sabourin, L.3
Rudnicki, M.4
Penn, L.5
Sadowski, I.6
-
32
-
-
0031547955
-
A RNA polymerase III-based two-hybrid system to study RNA polymerase II transcriptional regulators
-
Marsolier M.C., Prioleau M.N., and Sentenac A. A RNA polymerase III-based two-hybrid system to study RNA polymerase II transcriptional regulators. J Mol Biol 268 (1997) 243-249
-
(1997)
J Mol Biol
, vol.268
, pp. 243-249
-
-
Marsolier, M.C.1
Prioleau, M.N.2
Sentenac, A.3
-
33
-
-
0035131574
-
Two-hybrid selection assay to identify proteins interacting with polymerase II transcription factors and regulators
-
300, 302
-
Petrascheck M., Castagna F., and Barberis A. Two-hybrid selection assay to identify proteins interacting with polymerase II transcription factors and regulators. Biotechniques 30 (2001) 296-298 300, 302
-
(2001)
Biotechniques
, vol.30
, pp. 296-298
-
-
Petrascheck, M.1
Castagna, F.2
Barberis, A.3
-
34
-
-
0031051417
-
Mammalian two-hybrid system: a complementary approach to the yeast two-hybrid system
-
Luo Y., Batalao A., Zhou H., and Zhu L. Mammalian two-hybrid system: a complementary approach to the yeast two-hybrid system. Biotechniques 22 (1997) 350-352
-
(1997)
Biotechniques
, vol.22
, pp. 350-352
-
-
Luo, Y.1
Batalao, A.2
Zhou, H.3
Zhu, L.4
-
35
-
-
40049090424
-
High-throughput mammalian two-hybrid screening for protein-protein interactions using transfected cell arrays
-
This paper describes the application of transfected cell arrays for mammalian two-hybrid screens. The arrays contain immobilized bait and prey expression plasmids and an auto-fluorescent reporter construct. Adherent cells growing in the array pick the plasmids up and will become fluorescent if the expressed proteins interact.
-
Fiebitz A., Nyarsik L., Haendler B., Hu Y.H., Wagner F., Thamm S., Lehrach H., Janitz M., and Vanhecke D. High-throughput mammalian two-hybrid screening for protein-protein interactions using transfected cell arrays. BMC Genomics 9 (2008) 68. This paper describes the application of transfected cell arrays for mammalian two-hybrid screens. The arrays contain immobilized bait and prey expression plasmids and an auto-fluorescent reporter construct. Adherent cells growing in the array pick the plasmids up and will become fluorescent if the expressed proteins interact.
-
(2008)
BMC Genomics
, vol.9
, pp. 68
-
-
Fiebitz, A.1
Nyarsik, L.2
Haendler, B.3
Hu, Y.H.4
Wagner, F.5
Thamm, S.6
Lehrach, H.7
Janitz, M.8
Vanhecke, D.9
-
36
-
-
34347374044
-
Universal strategies in research and drug discovery based on protein-fragment complementation assays
-
Michnick S.W., Ear P.H., Manderson E.N., Remy I., and Stefan E. Universal strategies in research and drug discovery based on protein-fragment complementation assays. Nat Rev Drug Discov 6 (2007) 569-582
-
(2007)
Nat Rev Drug Discov
, vol.6
, pp. 569-582
-
-
Michnick, S.W.1
Ear, P.H.2
Manderson, E.N.3
Remy, I.4
Stefan, E.5
-
37
-
-
33751217186
-
Monitoring regulated protein-protein interactions using split TEV
-
A PPI between a bait and prey reconstitutes a TEV protease that subsequently releases a sequestered reporter protein in an irreversible manner, thus generating a stable readout for the PPI. The reporter protein is either a transcription factor or the light emitting enzyme luciferase.
-
Wehr M.C., Laage R., Bolz U., Fischer T.M., Grunewald S., Scheek S., Bach A., Nave K.A., and Rossner M.J. Monitoring regulated protein-protein interactions using split TEV. Nat Methods 3 (2006) 985-993. A PPI between a bait and prey reconstitutes a TEV protease that subsequently releases a sequestered reporter protein in an irreversible manner, thus generating a stable readout for the PPI. The reporter protein is either a transcription factor or the light emitting enzyme luciferase.
-
(2006)
Nat Methods
, vol.3
, pp. 985-993
-
-
Wehr, M.C.1
Laage, R.2
Bolz, U.3
Fischer, T.M.4
Grunewald, S.5
Scheek, S.6
Bach, A.7
Nave, K.A.8
Rossner, M.J.9
-
38
-
-
0035199166
-
Design and application of a cytokine-receptor-based interaction trap
-
Eyckerman S., Verhee A., der Heyden J.V., Lemmens I., Ostade X.V., Vandekerckhove J., and Tavernier J. Design and application of a cytokine-receptor-based interaction trap. Nat Cell Biol 3 (2001) 1114-1119
-
(2001)
Nat Cell Biol
, vol.3
, pp. 1114-1119
-
-
Eyckerman, S.1
Verhee, A.2
der Heyden, J.V.3
Lemmens, I.4
Ostade, X.V.5
Vandekerckhove, J.6
Tavernier, J.7
-
39
-
-
40149083727
-
MAPPIT analysis of early Toll-like receptor signalling events
-
Ulrichts P., and Tavernier J. MAPPIT analysis of early Toll-like receptor signalling events. Immunol Lett (2008)
-
(2008)
Immunol Lett
-
-
Ulrichts, P.1
Tavernier, J.2
-
40
-
-
33745510082
-
A complex interaction pattern of CIS and SOCS2 with the leptin receptor
-
Lavens D., Montoye T., Piessevaux J., Zabeau L., Vandekerckhove J., Gevaert K., Becker W., Eyckerman S., and Tavernier J. A complex interaction pattern of CIS and SOCS2 with the leptin receptor. J Cell Sci 119 (2006) 2214-2224
-
(2006)
J Cell Sci
, vol.119
, pp. 2214-2224
-
-
Lavens, D.1
Montoye, T.2
Piessevaux, J.3
Zabeau, L.4
Vandekerckhove, J.5
Gevaert, K.6
Becker, W.7
Eyckerman, S.8
Tavernier, J.9
-
41
-
-
42149089479
-
The yeast two-hybrid system and its role in drug discovery
-
Lentze N., and Auerbach D. The yeast two-hybrid system and its role in drug discovery. Expert Opin Ther Targets 12 (2008) 505-515
-
(2008)
Expert Opin Ther Targets
, vol.12
, pp. 505-515
-
-
Lentze, N.1
Auerbach, D.2
-
42
-
-
0029809862
-
Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions
-
Vidal M., Brachmann R.K., Fattaey A., Harlow E., and Boeke J.D. Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions. Proc Natl Acad Sci U S A 93 (1996) 10315-10320
-
(1996)
Proc Natl Acad Sci U S A
, vol.93
, pp. 10315-10320
-
-
Vidal, M.1
Brachmann, R.K.2
Fattaey, A.3
Harlow, E.4
Boeke, J.D.5
-
43
-
-
0031472251
-
A yeast genetic system for selecting small molecule inhibitors of protein-protein interactions in nanodroplets
-
Huang J., and Schreiber S.L. A yeast genetic system for selecting small molecule inhibitors of protein-protein interactions in nanodroplets. Proc Natl Acad Sci U S A 94 (1997) 13396-13401
-
(1997)
Proc Natl Acad Sci U S A
, vol.94
, pp. 13396-13401
-
-
Huang, J.1
Schreiber, S.L.2
-
44
-
-
34250674950
-
Identification of protein interaction antagonists using the repressed transactivator two-hybrid system
-
Joshi P.B., Hirst M., Malcolm T., Parent J., Mitchell D., Lund K., and Sadowski I. Identification of protein interaction antagonists using the repressed transactivator two-hybrid system. Biotechniques 42 (2007) 635-644
-
(2007)
Biotechniques
, vol.42
, pp. 635-644
-
-
Joshi, P.B.1
Hirst, M.2
Malcolm, T.3
Parent, J.4
Mitchell, D.5
Lund, K.6
Sadowski, I.7
-
45
-
-
21444437387
-
Reverse MAPPIT: screening for protein-protein interaction modifiers in mammalian cells
-
Eyckerman S., Lemmens I., Catteeuw D., Verhee A., Vandekerckhove J., Lievens S., and Tavernier J. Reverse MAPPIT: screening for protein-protein interaction modifiers in mammalian cells. Nat Methods 2 (2005) 427-433
-
(2005)
Nat Methods
, vol.2
, pp. 427-433
-
-
Eyckerman, S.1
Lemmens, I.2
Catteeuw, D.3
Verhee, A.4
Vandekerckhove, J.5
Lievens, S.6
Tavernier, J.7
-
46
-
-
0029776689
-
A three-hybrid system to detect RNA-protein interactions in vivo
-
SenGupta D.J., Zhang B., Kraemer B., Pochart P., Fields S., and Wickens M. A three-hybrid system to detect RNA-protein interactions in vivo. Proc Natl Acad Sci U S A 93 (1996) 8496-8501
-
(1996)
Proc Natl Acad Sci U S A
, vol.93
, pp. 8496-8501
-
-
SenGupta, D.J.1
Zhang, B.2
Kraemer, B.3
Pochart, P.4
Fields, S.5
Wickens, M.6
-
47
-
-
0029824170
-
A three-hybrid system for detecting small ligand-protein receptor interactions
-
Licitra E.J., and Liu J.O. A three-hybrid system for detecting small ligand-protein receptor interactions. Proc Natl Acad Sci U S A 93 (1996) 12817-12821
-
(1996)
Proc Natl Acad Sci U S A
, vol.93
, pp. 12817-12821
-
-
Licitra, E.J.1
Liu, J.O.2
-
48
-
-
12244298884
-
An optimized dexamethasone-methotrexate yeast 3-hybrid system for high-throughput screening of small molecule-protein interactions
-
Baker K., Sengupta D., Salazar-Jimenez G., and Cornish V.W. An optimized dexamethasone-methotrexate yeast 3-hybrid system for high-throughput screening of small molecule-protein interactions. Anal Biochem 315 (2003) 134-137
-
(2003)
Anal Biochem
, vol.315
, pp. 134-137
-
-
Baker, K.1
Sengupta, D.2
Salazar-Jimenez, G.3
Cornish, V.W.4
-
49
-
-
1542290057
-
A three-hybrid approach to scanning the proteome for targets of small molecule kinase inhibitors
-
Becker F., Murthi K., Smith C., Come J., Costa-Roldan N., Kaufmann C., Hanke U., Degenhart C., Baumann S., Wallner W., et al. A three-hybrid approach to scanning the proteome for targets of small molecule kinase inhibitors. Chem Biol 11 (2004) 211-223
-
(2004)
Chem Biol
, vol.11
, pp. 211-223
-
-
Becker, F.1
Murthi, K.2
Smith, C.3
Come, J.4
Costa-Roldan, N.5
Kaufmann, C.6
Hanke, U.7
Degenhart, C.8
Baumann, S.9
Wallner, W.10
-
50
-
-
33745311964
-
A small-molecule-protein interaction system with split-ubiquitin as sensor
-
Dirnberger D., Unsin G., Schlenker S., and Reichel C. A small-molecule-protein interaction system with split-ubiquitin as sensor. Chembiochem 7 (2006) 936-942
-
(2006)
Chembiochem
, vol.7
, pp. 936-942
-
-
Dirnberger, D.1
Unsin, G.2
Schlenker, S.3
Reichel, C.4
-
51
-
-
33746329294
-
MASPIT: three-hybrid trap for quantitative proteome fingerprinting of small molecule-protein interactions in mammalian cells
-
Caligiuri M., Molz L., Liu Q., Kaplan F., Xu J.P., Majeti J.Z., Ramos-Kelsey R., Murthi K., Lievens S., Tavernier J., et al. MASPIT: three-hybrid trap for quantitative proteome fingerprinting of small molecule-protein interactions in mammalian cells. Chem Biol 13 (2006) 711-722
-
(2006)
Chem Biol
, vol.13
, pp. 711-722
-
-
Caligiuri, M.1
Molz, L.2
Liu, Q.3
Kaplan, F.4
Xu, J.P.5
Majeti, J.Z.6
Ramos-Kelsey, R.7
Murthi, K.8
Lievens, S.9
Tavernier, J.10
-
52
-
-
34147108830
-
A novel approach for generating full-length, high coverage allele libraries for the analysis of protein interactions
-
Gray P.N., Busser K.J., and Chappell T.G. A novel approach for generating full-length, high coverage allele libraries for the analysis of protein interactions. Mol Cell Proteomics 6 (2007) 514-526
-
(2007)
Mol Cell Proteomics
, vol.6
, pp. 514-526
-
-
Gray, P.N.1
Busser, K.J.2
Chappell, T.G.3
-
53
-
-
35648977580
-
One- plus two-hybrid system, a novel yeast genetic selection for specific missense mutations disrupting protein/protein interactions
-
In a first one-hybrid step, informative mutants are selected from randomly generated mutant alleles of one interacting partner (prey). The prey construct contains an additional DBD at its C-terminus, and therefore only full-length proteins can activate the HIS3 reporter gene and grow. In the second two-hybrid step, the selected alleles are tested for interaction with the bait protein by β-gelactosidase expression. Alleles that grow on -His medium not expressing β-galactosidase selectively abolish the bait-prey interaction.
-
Kim J.Y., Park O.G., Lee J.W., and Lee Y.C. One- plus two-hybrid system, a novel yeast genetic selection for specific missense mutations disrupting protein/protein interactions. Mol Cell Proteomics 6 (2007) 1727-1740. In a first one-hybrid step, informative mutants are selected from randomly generated mutant alleles of one interacting partner (prey). The prey construct contains an additional DBD at its C-terminus, and therefore only full-length proteins can activate the HIS3 reporter gene and grow. In the second two-hybrid step, the selected alleles are tested for interaction with the bait protein by β-gelactosidase expression. Alleles that grow on -His medium not expressing β-galactosidase selectively abolish the bait-prey interaction.
-
(2007)
Mol Cell Proteomics
, vol.6
, pp. 1727-1740
-
-
Kim, J.Y.1
Park, O.G.2
Lee, J.W.3
Lee, Y.C.4
-
54
-
-
39049093111
-
Mapping of protein-protein interaction sites by the 'absence of interference' approach
-
The interaction region for a PPI on the protein surface can be identified by an absence or depletion of mutations. The mutations are mapped on the surface of the protein structure.
-
Dhayalan A., Jurkowski T.P., Laser H., Reinhardt R., Jia D., Cheng X., and Jeltsch A. Mapping of protein-protein interaction sites by the 'absence of interference' approach. J Mol Biol 376 (2008) 1091-1099. The interaction region for a PPI on the protein surface can be identified by an absence or depletion of mutations. The mutations are mapped on the surface of the protein structure.
-
(2008)
J Mol Biol
, vol.376
, pp. 1091-1099
-
-
Dhayalan, A.1
Jurkowski, T.P.2
Laser, H.3
Reinhardt, R.4
Jia, D.5
Cheng, X.6
Jeltsch, A.7
-
55
-
-
34548838274
-
Phage display for engineering and analyzing protein interaction interfaces
-
Sidhu S.S., and Koide S. Phage display for engineering and analyzing protein interaction interfaces. Curr Opin Struct Biol 17 (2007) 481-487
-
(2007)
Curr Opin Struct Biol
, vol.17
, pp. 481-487
-
-
Sidhu, S.S.1
Koide, S.2
-
56
-
-
33750953227
-
Whole-genome re-sequencing
-
Bentley D.R. Whole-genome re-sequencing. Curr Opin Genet Dev 16 (2006) 545-552
-
(2006)
Curr Opin Genet Dev
, vol.16
, pp. 545-552
-
-
Bentley, D.R.1
-
57
-
-
0030923133
-
Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions
-
Aronheim A., Zandi E., Hennemann H., Elledge S.J., and Karin M. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol Cell Biol 17 (1997) 3094-3102
-
(1997)
Mol Cell Biol
, vol.17
, pp. 3094-3102
-
-
Aronheim, A.1
Zandi, E.2
Hennemann, H.3
Elledge, S.J.4
Karin, M.5
-
58
-
-
0032497564
-
The ras recruitment system, a novel approach to the study of protein-protein interactions
-
Broder Y.C., Katz S., and Aronheim A. The ras recruitment system, a novel approach to the study of protein-protein interactions. Curr Biol 8 (1998) 1121-1124
-
(1998)
Curr Biol
, vol.8
, pp. 1121-1124
-
-
Broder, Y.C.1
Katz, S.2
Aronheim, A.3
-
59
-
-
32944457836
-
Yeast growth selection system for detecting activity and inhibition of dimerization-dependent receptor tyrosine kinase
-
Gunde T., and Barberis A. Yeast growth selection system for detecting activity and inhibition of dimerization-dependent receptor tyrosine kinase. Biotechniques 39 (2005) 541-549
-
(2005)
Biotechniques
, vol.39
, pp. 541-549
-
-
Gunde, T.1
Barberis, A.2
-
60
-
-
0036852292
-
Detection of peptides, proteins, and drugs that selectively interact with protein targets
-
Serebriiskii I.G., Mitina O., Pugacheva E.N., Benevolenskaya E., Kotova E., Toby G.G., Khazak V., Kaelin W.G., Chernoff J., and Golemis E.A. Detection of peptides, proteins, and drugs that selectively interact with protein targets. Genome Res 12 (2002) 1785-1791
-
(2002)
Genome Res
, vol.12
, pp. 1785-1791
-
-
Serebriiskii, I.G.1
Mitina, O.2
Pugacheva, E.N.3
Benevolenskaya, E.4
Kotova, E.5
Toby, G.G.6
Khazak, V.7
Kaelin, W.G.8
Chernoff, J.9
Golemis, E.A.10
-
61
-
-
3042780219
-
A tethered catalysis, two-hybrid system to identify protein-protein interactions requiring post-translational modifications
-
Guo D., Hazbun T.R., Xu X.J., Ng S.L., Fields S., and Kuo M.H. A tethered catalysis, two-hybrid system to identify protein-protein interactions requiring post-translational modifications. Nat Biotechnol 22 (2004) 888-892
-
(2004)
Nat Biotechnol
, vol.22
, pp. 888-892
-
-
Guo, D.1
Hazbun, T.R.2
Xu, X.J.3
Ng, S.L.4
Fields, S.5
Kuo, M.H.6
-
62
-
-
0033776891
-
Use of G-protein fusions to monitor integral membrane protein-protein interactions in yeast
-
Ehrhard K.N., Jacoby J.J., Fu X.Y., Jahn R., and Dohlman H.G. Use of G-protein fusions to monitor integral membrane protein-protein interactions in yeast. Nat Biotechnol 18 (2000) 1075-1079
-
(2000)
Nat Biotechnol
, vol.18
, pp. 1075-1079
-
-
Ehrhard, K.N.1
Jacoby, J.J.2
Fu, X.Y.3
Jahn, R.4
Dohlman, H.G.5
-
63
-
-
0037767632
-
Cell growth selection system to detect extracellular and transmembrane protein interactions
-
Urech D.M., Lichtlen P., and Barberis A. Cell growth selection system to detect extracellular and transmembrane protein interactions. Biochim Biophys Acta 1622 (2003) 117-127
-
(2003)
Biochim Biophys Acta
, vol.1622
, pp. 117-127
-
-
Urech, D.M.1
Lichtlen, P.2
Barberis, A.3
-
64
-
-
0034691202
-
A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions
-
Joung J.K., Ramm E.I., and Pabo C.O. A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proc Natl Acad Sci U S A 97 (2000) 7382-7387
-
(2000)
Proc Natl Acad Sci U S A
, vol.97
, pp. 7382-7387
-
-
Joung, J.K.1
Ramm, E.I.2
Pabo, C.O.3
-
65
-
-
21044452487
-
A combined yeast/bacteria two-hybrid system: development and evaluation
-
Serebriiskii I.G., Fang R., Latypova E., Hopkins R., Vinson C., Joung J.K., and Golemis E.A. A combined yeast/bacteria two-hybrid system: development and evaluation. Mol Cell Proteomics 4 (2005) 819-826
-
(2005)
Mol Cell Proteomics
, vol.4
, pp. 819-826
-
-
Serebriiskii, I.G.1
Fang, R.2
Latypova, E.3
Hopkins, R.4
Vinson, C.5
Joung, J.K.6
Golemis, E.A.7
-
66
-
-
0027745644
-
Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system
-
Li J.J., and Herskowitz I. Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system. Science 262 (1993) 1870-1874
-
(1993)
Science
, vol.262
, pp. 1870-1874
-
-
Li, J.J.1
Herskowitz, I.2
-
67
-
-
33744972290
-
A gene-centered C. elegans protein-DNA interaction network
-
Deplancke B., Mukhopadhyay A., Ao W., Elewa A.M., Grove C.A., Martinez N.J., Sequerra R., Doucette-Stamm L., Reece-Hoyes J.S., Hope I.A., et al. A gene-centered C. elegans protein-DNA interaction network. Cell 125 (2006) 1193-1205
-
(2006)
Cell
, vol.125
, pp. 1193-1205
-
-
Deplancke, B.1
Mukhopadhyay, A.2
Ao, W.3
Elewa, A.M.4
Grove, C.A.5
Martinez, N.J.6
Sequerra, R.7
Doucette-Stamm, L.8
Reece-Hoyes, J.S.9
Hope, I.A.10
|