Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions

Proceedings of the National Academy of Sciences of the United States of America

Volumn 93, Issue 19, 1996, Pages 10315-10320

  Vidal, Marc ^a   Brachmann, Rainer K ^b,c   Fattaey, Ali ^d   Harlow, Ed ^a   Boeke, Jef D ^b  

^a MASSACHUSETTS GENERAL HOSPITAL CANCER CENTER   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c JOHNS HOPKINS UNIVERSITY   (United States)

^d ONYX PHARMACEUTICALS   (United States)

Author keywords

5 fluoroorotic acid; negative selection; URA3

Indexed keywords

DNA; DNA BINDING PROTEIN; FLUOROOROTIC ACID;

ARTICLE; BINDING SITE; CONTROLLED STUDY; DISSOCIATION; HYBRID; IMMUNOBLOTTING; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN PROTEIN INTERACTION; REPORTER GENE; SACCHAROMYCES CEREVISIAE;

BASE SEQUENCE; BINDING SITES; BLOTTING, WESTERN; CONSENSUS SEQUENCE; DNA PRIMERS; DNA, FUNGAL; DNA-BINDING PROTEINS; FUNGAL PROTEINS; GENES, REPORTER; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); PROTEIN HYBRIDIZATION; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS;

SACCHAROMYCES CEREVISIAE;

EID: 0029809862     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.93.19.10315     Document Type: Article

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