An optimized dexamethasone-methotrexate yeast 3-hybrid system for high-throughput screening of small molecule-protein interactions

Analytical Biochemistry

Volumn 315, Issue 1, 2003, Pages 134-137

  Baker, Kathleen ^a   Sengupta, Debleena ^a   Salazar Jimenez, Gilda ^a   Cornish, Virginia W ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

DEXAMETHASONES; HIGH THROUGHPUT SCREENING; PROTEIN INTERACTION; SMALL MOLECULES;

DRUG PRODUCTS;

BETA GALACTOSIDASE; DEXAMETHASONE; DIHYDROFOLATE REDUCTASE; HYBRID PROTEIN; METHOTREXATE;

ARTICLE; CELL HYBRIDIZATION; CONTROLLED STUDY; DIMERIZATION; DNA BINDING MOTIF; ENZYME ACTIVITY; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN INTERACTION; YEAST;

EID: 12244298884     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00698-X     Document Type: Article

References (6)

1. Lin H., Abida W., Sauer R., Cornish V. J. Am. Chem. Soc. 122:2000;4247-4248.
2. Gyuris J., Golemis E., Chertkov H., Brent R. Cell. 75:1993;791-803.
3. Adams A., Gottschling D., Kaiser C., Stearns T. Methods in Yeast Genetics. 1998;Cold Spring Harbor Laboratory Press, Plainview, New York.
4. Abida W., Carter B., Althoff E., Lin H., Cornish V. ChemBioChem. 3:2002;101-109.
5. Estojak J., Brent R., Golemis E. Mol. Cell. Biol. 15:1995;5820-5829.
6. Watson M., Buckholz R., Weiner M. Biotechniques. 21:1996;255-259.