Two-hybrid selection assay to identify proteins interacting with polymerase II transcription factors and regulators

BioTechniques

Volumn 30, Issue 2, 2001, Pages 296-302

  Petrascheck, M ^a   Castagna, F ^a   Barberis, A ^a  

^a ESBATech AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE II; SMALL NUCLEAR RNA; TRANSCRIPTION FACTOR;

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENE LIBRARY; NONHUMAN; PHENOTYPE; PLASMID; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; REACTION ANALYSIS; SCREENING TEST; TEMPERATURE DEPENDENCE; TWO HYBRID SYSTEM; YEAST;

EID: 0035131574     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01302st02     Document Type: Article

References (14)

1. Recruitment of the RNA polymerase II holoenzyme and its implications in gene regulation
2. Contact with a component of the polymerase II holoenzyme suffices for gene activation
3. Gene activation by recruitment of the RNA polymerase II holoenzyme
4. A novel genetic system to detect protein-protein interactions
5. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure
6. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites
7. A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome
8. Directing transcription of an RNA polymerase III gene via GAL4 sites
9. A RNA polymerase III-based two-hybrid system to study RNA polymerase II transcriptional regulators
10. Reciprocal interference between nucleosomal organization and transcriptional activity of the yeast SNR6 gene
11. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae
12. Yeast forward and reverse "n"-hybrid systems
13. Protein interaction mapping in C. elegans using proteins involved in vulval development