-
1
-
-
0017121112
-
Enzymatic unwinding of DNA. 1. Purification and characterization of a DNA-dependent ATPase from Escherichia coli
-
Abdel-Monem, M. & Hoffmann-Berling, H. Enzymatic unwinding of DNA. 1. Purification and characterization of a DNA-dependent ATPase from Escherichia coli. Eur. J. Biochem. 65, 431-440 (1976).
-
(1976)
Eur. J. Biochem
, vol.65
, pp. 431-440
-
-
Abdel-Monem, M.1
Hoffmann-Berling, H.2
-
2
-
-
0017113549
-
Enzymic unwinding of DNA. 2. Chain separation by an ATP-dependent DNA unwinding enzyme
-
Abdel-Monem, M., Durwald, H. & Hoffmann-Berling, H. Enzymic unwinding of DNA. 2. Chain separation by an ATP-dependent DNA unwinding enzyme. Eur. J. Biochem. 65, 441-449 (1976).
-
(1976)
Eur. J. Biochem
, vol.65
, pp. 441-449
-
-
Abdel-Monem, M.1
Durwald, H.2
Hoffmann-Berling, H.3
-
3
-
-
0017759232
-
DNA unwinding enzyme II of Escherichia coli. 2. Characterization of the DNA unwinding activity
-
Abdel-Monem, M., Durwald, H. & Hoffmann-Berling, H. DNA unwinding enzyme II of Escherichia coli. 2. Characterization of the DNA unwinding activity. Eur. J. Biochem. 79, 39-45 (1977).
-
(1977)
Eur. J. Biochem
, vol.79
, pp. 39-45
-
-
Abdel-Monem, M.1
Durwald, H.2
Hoffmann-Berling, H.3
-
4
-
-
0019363395
-
Proteins controlling the helical structure of DNA
-
Geider, K. & Hoffmann-Berling, H. Proteins controlling the helical structure of DNA. Annu. Rev. Biochem. 50, 233-260 (1981).
-
(1981)
Annu. Rev. Biochem
, vol.50
, pp. 233-260
-
-
Geider, K.1
Hoffmann-Berling, H.2
-
5
-
-
0017130522
-
Mechanism of DNA degradation by the ATP-dependent DNase from Hemophilus influenzae Rd
-
Wilcox, K. W. & Smith, H. O. Mechanism of DNA degradation by the ATP-dependent DNase from Hemophilus influenzae Rd. J. Biol. Chem. 251, 6127-6134 (1976).
-
(1976)
J. Biol. Chem
, vol.251
, pp. 6127-6134
-
-
Wilcox, K.W.1
Smith, H.O.2
-
6
-
-
0017332435
-
A mechanism of duplex DNA replication revealed by enzymatic studies of phage φ X174: Catalytic strand separation in advance of replication
-
Scott, J. F., Eisenberg, S., Bertsch, L. L. & Kornberg, A. A mechanism of duplex DNA replication revealed by enzymatic studies of phage φ X174: catalytic strand separation in advance of replication. Proc. Natl Acad. Sci. USA 74, 193-197 (1977).
-
(1977)
Proc. Natl Acad. Sci. USA
, vol.74
, pp. 193-197
-
-
Scott, J.F.1
Eisenberg, S.2
Bertsch, L.L.3
Kornberg, A.4
-
7
-
-
0017900277
-
ATP utilization by rep protein in the catalytic separation of DNA strands at a replicating fork
-
Kornberg, A., Scott, J. F. & Bertsch, L. L. ATP utilization by rep protein in the catalytic separation of DNA strands at a replicating fork. J. Biol. Chem. 253, 3298-3304 (1978).
-
(1978)
J. Biol. Chem
, vol.253
, pp. 3298-3304
-
-
Kornberg, A.1
Scott, J.F.2
Bertsch, L.L.3
-
8
-
-
0018645477
-
Enzyme-catalyzed DNA unwinding. A DNA-dependent ATPase from E. coli
-
Yarranton, G. T., Das, R. H. & Gefter, M. L. Enzyme-catalyzed DNA unwinding. A DNA-dependent ATPase from E. coli. J. Biol. Chem. 254, 11997-12001 (1979).
-
(1979)
J. Biol. Chem
, vol.254
, pp. 11997-12001
-
-
Yarranton, G.T.1
Das, R.H.2
Gefter, M.L.3
-
9
-
-
0018353521
-
Enzyme-catalyzed DNA unwinding: Studies on Escherichia coli rep protein
-
Yarranton, G. T. & Gefter, M. L. Enzyme-catalyzed DNA unwinding: studies on Escherichia coli rep protein. Proc. Natl Acad. Sci. USA 76, 1658-1662 (1979).
-
(1979)
Proc. Natl Acad. Sci. USA
, vol.76
, pp. 1658-1662
-
-
Yarranton, G.T.1
Gefter, M.L.2
-
10
-
-
0018352189
-
The rep protein of Escherichia coli: Interaction with DNA and other proteins
-
Duguet, M., Yarranton, G. & Gefter, M. The rep protein of Escherichia coli: interaction with DNA and other proteins. Cold Spring Harb. Symp. Quant. Biol. 43, 335-343 (1979).
-
(1979)
Cold Spring Harb. Symp. Quant. Biol
, vol.43
, pp. 335-343
-
-
Duguet, M.1
Yarranton, G.2
Gefter, M.3
-
11
-
-
25144500083
-
A fork-clearing role for UvrD
-
Flores, M. J., Sanchez, N. & Michel, B. A fork-clearing role for UvrD. Mol. Microbiol. 57, 1664-1675 (2005).
-
(2005)
Mol. Microbiol
, vol.57
, pp. 1664-1675
-
-
Flores, M.J.1
Sanchez, N.2
Michel, B.3
-
12
-
-
34249938016
-
Recombination proteins and rescue of arrested replication forks
-
Michel, B., Boubakri, H., Baharoglu, Z., Lemasson, M. & Lestini, R. Recombination proteins and rescue of arrested replication forks. DNA Repair (Amst.) 6, 967-980 (2007).
-
(2007)
DNA Repair (Amst.)
, vol.6
, pp. 967-980
-
-
Michel, B.1
Boubakri, H.2
Baharoglu, Z.3
Lemasson, M.4
Lestini, R.5
-
13
-
-
0037673943
-
The Srs2 helicase prevents recombination by disrupting Rad51 nucleoprotein filaments
-
Veaute, X. et al. The Srs2 helicase prevents recombination by disrupting Rad51 nucleoprotein filaments. Nature 423, 309-312 (2003).
-
(2003)
Nature
, vol.423
, pp. 309-312
-
-
Veaute, X.1
-
14
-
-
13244252309
-
UvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli
-
The anti-recombinogenic activity of UvrD is shown to be the probable result of its ability to displace the RecA protein from ssDNA
-
Veaute, X. et al. UvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli. EMBO J. 24, 180-189 (2005). The anti-recombinogenic activity of UvrD is shown to be the probable result of its ability to displace the RecA protein from ssDNA.
-
(2005)
EMBO J
, vol.24
, pp. 180-189
-
-
Veaute, X.1
-
15
-
-
34548061530
-
UvrD controls the access of recombination proteins to blocked replication forks
-
Lestini, R. & Michel, B. UvrD controls the access of recombination proteins to blocked replication forks. EMBO J. 26, 3804-3814 (2007).
-
(2007)
EMBO J
, vol.26
, pp. 3804-3814
-
-
Lestini, R.1
Michel, B.2
-
16
-
-
0035808566
-
Active disruption of an RNA-protein interaction by a DExH/D RNA helicase
-
Jankowsky, E., Gross, C. H., Shuman, S. & Pyle, A. M. Active disruption of an RNA-protein interaction by a DExH/D RNA helicase. Science 291, 121-125 (2001).
-
(2001)
Science
, vol.291
, pp. 121-125
-
-
Jankowsky, E.1
Gross, C.H.2
Shuman, S.3
Pyle, A.M.4
-
17
-
-
33745790132
-
Chromatin remodelling: The industrial revolution of DNA around histones
-
Saha, A., Wittmeyer, J. & Cairns, B. R. Chromatin remodelling: the industrial revolution of DNA around histones. Nature Rev. Mol. Cell Biol. 7, 437-447 (2006).
-
(2006)
Nature Rev. Mol. Cell Biol
, vol.7
, pp. 437-447
-
-
Saha, A.1
Wittmeyer, J.2
Cairns, B.R.3
-
18
-
-
33344473656
-
The DEAD-box protein family of RNA helicases
-
Cordin, O., Banroques, J., Tanner, N. K. & Linder, P. The DEAD-box protein family of RNA helicases. Gene 367, 17-37 (2006).
-
(2006)
Gene
, vol.367
, pp. 17-37
-
-
Cordin, O.1
Banroques, J.2
Tanner, N.K.3
Linder, P.4
-
19
-
-
0030861685
-
DNA helicases in inherited human disorders
-
Ellis, N. A. DNA helicases in inherited human disorders. Curr. Opin. Genet. Dev. 7, 354-363 (1997).
-
(1997)
Curr. Opin. Genet. Dev
, vol.7
, pp. 354-363
-
-
Ellis, N.A.1
-
20
-
-
28844442307
-
Roles of the Bloom's syndrome helicase in the maintenance of genome stability
-
Cheok, C. F. et al. Roles of the Bloom's syndrome helicase in the maintenance of genome stability. Biochem. Soc. Trans. 33, 1456-1459 (2005).
-
(2005)
Biochem. Soc. Trans
, vol.33
, pp. 1456-1459
-
-
Cheok, C.F.1
-
21
-
-
0033652180
-
Werner's syndrome: From clinics to genetics
-
Goto, M. Werner's syndrome: from clinics to genetics. Clin. Exp. Rheumatol. 18, 760-766 (2000).
-
(2000)
Clin. Exp. Rheumatol
, vol.18
, pp. 760-766
-
-
Goto, M.1
-
22
-
-
34247220441
-
New insights into the combined Cockayne/xeroderma pigmentosum complex: Human XPG protein can function in transcription factor stability
-
Friedberg, E. C. & Wood, R. D. New insights into the combined Cockayne/xeroderma pigmentosum complex: human XPG protein can function in transcription factor stability. Mol. Cell 26, 162-164 (2007).
-
(2007)
Mol. Cell
, vol.26
, pp. 162-164
-
-
Friedberg, E.C.1
Wood, R.D.2
-
23
-
-
0033786801
-
Structure and function of hexameric helicases
-
Patel, S. S. & Picha, K. M. Structure and function of hexameric helicases. Annu. Rev. Biochem. 69, 651-697 (2000).
-
(2000)
Annu. Rev. Biochem
, vol.69
, pp. 651-697
-
-
Patel, S.S.1
Picha, K.M.2
-
24
-
-
34548638261
-
-
Singleton, M. R., Dillingham, M. S. & Wigley, D. B. Structure and mechanism of helicases and nucleic acid translocases. Annu. Rev. Biochem. 76, 23-50 (2007). Structures and mechanistic models, including the inch-worm model, are reviewed for all classes of helicase.
-
Singleton, M. R., Dillingham, M. S. & Wigley, D. B. Structure and mechanism of helicases and nucleic acid translocases. Annu. Rev. Biochem. 76, 23-50 (2007). Structures and mechanistic models, including the inch-worm model, are reviewed for all classes of helicase.
-
-
-
-
25
-
-
0027182114
-
Helicases: Amino acid sequence comparisons and structure-function relationships
-
Gorbalenya, A. E. & Koonin, E. V. Helicases: amino acid sequence comparisons and structure-function relationships. Curr. Opin. Struct. Biol. 3, 419-429 (1993).
-
(1993)
Curr. Opin. Struct. Biol
, vol.3
, pp. 419-429
-
-
Gorbalenya, A.E.1
Koonin, E.V.2
-
26
-
-
33749139723
-
Dead-box proteins: A family affair - active and passive players in RNP-remodeling
-
Linder, P. Dead-box proteins: a family affair - active and passive players in RNP-remodeling. Nucleic Acids Res. 34, 4168-4180 (2006).
-
(2006)
Nucleic Acids Res
, vol.34
, pp. 4168-4180
-
-
Linder, P.1
-
27
-
-
34347385000
-
RNA helicases - one fold for many functions
-
Jankowsky, E. & Fairman, M. E. RNA helicases - one fold for many functions. Curr. Opin. Struct. Biol. 17, 316-324 (2007).
-
(2007)
Curr. Opin. Struct. Biol
, vol.17
, pp. 316-324
-
-
Jankowsky, E.1
Fairman, M.E.2
-
28
-
-
0033428970
-
Helicase motifs: The engine that powers DNA unwinding
-
Hall, M. C. & Matson, S. W. Helicase motifs: the engine that powers DNA unwinding. Mol. Microbiol. 34, 867-877 (1999).
-
(1999)
Mol. Microbiol
, vol.34
, pp. 867-877
-
-
Hall, M.C.1
Matson, S.W.2
-
29
-
-
2342443461
-
Protein displacement by DExH/D "RNA helicases" without duplex unwinding
-
Fairman, M. E. et al. Protein displacement by DExH/D "RNA helicases" without duplex unwinding. Science 304, 730-734 (2004).
-
(2004)
Science
, vol.304
, pp. 730-734
-
-
Fairman, M.E.1
-
30
-
-
0029856618
-
Crystal structure of a DExx box DNA helicase
-
Subramanya, H. S., Bird, L. E., Brannigan, J. A. & Wigley, D. B. Crystal structure of a DExx box DNA helicase. Nature 384, 379-383 (1996).
-
(1996)
Nature
, vol.384
, pp. 379-383
-
-
Subramanya, H.S.1
Bird, L.E.2
Brannigan, J.A.3
Wigley, D.B.4
-
31
-
-
0033515425
-
Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism
-
Velankar, S. S., Soultanas, P., Dillingham, M. S., Subramanya, H. S. & Wigley, D. B. Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism. Cell 97, 75-84 (1999).
-
(1999)
Cell
, vol.97
, pp. 75-84
-
-
Velankar, S.S.1
Soultanas, P.2
Dillingham, M.S.3
Subramanya, H.S.4
Wigley, D.B.5
-
32
-
-
0030740262
-
-
Korolev, S., Hsieh, J., Gauss, G. H., Lohman, T. M. & Waksman, G. Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP. Cell 90, 635-647 (1997).
-
Korolev, S., Hsieh, J., Gauss, G. H., Lohman, T. M. & Waksman, G. Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP. Cell 90, 635-647 (1997).
-
-
-
-
33
-
-
33845657428
-
-
Lee, J. Y. & Yang, W. UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke. Cell 127, 1349-1360 (2006). High resolution crystal structures of UvrD monomers bound to DNA substrates are used to propose a wrench and inch-worm mechanism for DNA unwinding by a UvrD monomer.
-
Lee, J. Y. & Yang, W. UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke. Cell 127, 1349-1360 (2006). High resolution crystal structures of UvrD monomers bound to DNA substrates are used to propose a wrench and inch-worm mechanism for DNA unwinding by a UvrD monomer.
-
-
-
-
34
-
-
9144271294
-
Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks
-
The crystal structure of the bipolar RecBCD helicase shows interactions between an accessory protein (RecC) and two SF1 helicases with opposite directionality, the 3′ to 5′ RecB and the 5′ to 3′ RecD
-
Singleton, M. R., Dillingham, M. S., Gaudier, M., Kowalczykowski, S. C. & Wigley, D. B. Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. Nature 432, 187-193 (2004). The crystal structure of the bipolar RecBCD helicase shows interactions between an accessory protein (RecC) and two SF1 helicases with opposite directionality, the 3′ to 5′ RecB and the 5′ to 3′ RecD.
-
(2004)
Nature
, vol.432
, pp. 187-193
-
-
Singleton, M.R.1
Dillingham, M.S.2
Gaudier, M.3
Kowalczykowski, S.C.4
Wigley, D.B.5
-
35
-
-
0035812836
-
Structural analysis of DNA replication fork reversal by RecG
-
Singleton, M. R., Scaife, S. & Wigley, D. B. Structural analysis of DNA replication fork reversal by RecG. Cell 107, 79-89 (2001).
-
(2001)
Cell
, vol.107
, pp. 79-89
-
-
Singleton, M.R.1
Scaife, S.2
Wigley, D.B.3
-
36
-
-
0030979410
-
Structure of the hepatitis C virus RNA helicase domain
-
Yao, N. et al. Structure of the hepatitis C virus RNA helicase domain. Nature Struct. Biol. 4, 463-467 (1997).
-
(1997)
Nature Struct. Biol
, vol.4
, pp. 463-467
-
-
Yao, N.1
-
37
-
-
0032518490
-
Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: The crystal structure provides insights into the mode of unwinding
-
Kim, J. L. et al. Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding. Structure 6, 89-100 (1998).
-
(1998)
Structure
, vol.6
, pp. 89-100
-
-
Kim, J.L.1
-
38
-
-
33645645084
-
Structural and biological identification of residues on the surface of NS3 helicase required for optimal replication of the hepatitis C virus
-
Mackintosh, S. G. et al. Structural and biological identification of residues on the surface of NS3 helicase required for optimal replication of the hepatitis C virus. J. Biol. Chem. 281, 3528-3535 (2006).
-
(2006)
J. Biol. Chem
, vol.281
, pp. 3528-3535
-
-
Mackintosh, S.G.1
-
39
-
-
0141865522
-
High-resolution structure of the E.coli RecQ helicase catalytic core
-
Bernstein, D. A., Zittel, M. C. & Keck, J. L. High-resolution structure of the E.coli RecQ helicase catalytic core. EMBO J. 22, 4910-4921 (2003).
-
(2003)
EMBO J
, vol.22
, pp. 4910-4921
-
-
Bernstein, D.A.1
Zittel, M.C.2
Keck, J.L.3
-
40
-
-
33646017369
-
Structural basis for RNA unwinding by the DEAD-box protein Drosophila Vasa
-
Sengoku, T., Nureki, O., Nakamura, A., Kobayashi, S. & Yokoyama, S. Structural basis for RNA unwinding by the DEAD-box protein Drosophila Vasa. Cell 125, 287-300 (2006).
-
(2006)
Cell
, vol.125
, pp. 287-300
-
-
Sengoku, T.1
Nureki, O.2
Nakamura, A.3
Kobayashi, S.4
Yokoyama, S.5
-
41
-
-
34447132375
-
Structural basis for DNA duplex separation by a superfamily-2 helicase
-
Buttner, K., Nehring, S. & Hopfner, K. P. Structural basis for DNA duplex separation by a superfamily-2 helicase. Nature Struct. Mol. Biol. 14, 647-652 (2007).
-
(2007)
Nature Struct. Mol. Biol
, vol.14
, pp. 647-652
-
-
Buttner, K.1
Nehring, S.2
Hopfner, K.P.3
-
42
-
-
0031031958
-
Kinetic measurement of the step-size of DNA unwinding by Escherichia coli UvrD helicase
-
Ali, J. A. & Lohman, T. M. Kinetic measurement of the step-size of DNA unwinding by Escherichia coli UvrD helicase. Science 275, 377-380 (1997).
-
(1997)
Science
, vol.275
, pp. 377-380
-
-
Ali, J.A.1
Lohman, T.M.2
-
43
-
-
0141865611
-
General methods for analysis of sequential "n-step" kinetic mechanisms: Application to single turnover kinetics of helicase-catalyzed DNA unwinding
-
The use of pre-steady-state kinetic methods to study DNA unwinding is discussed along with the concept of a kinetic step size
-
Lucius, A. L., Maluf, N. K., Fischer, C. J. & Lohman, T. M. General methods for analysis of sequential "n-step" kinetic mechanisms: application to single turnover kinetics of helicase-catalyzed DNA unwinding. Biophys. J. 85, 2224-2239 (2003). The use of pre-steady-state kinetic methods to study DNA unwinding is discussed along with the concept of a kinetic step size.
-
(2003)
Biophys. J
, vol.85
, pp. 2224-2239
-
-
Lucius, A.L.1
Maluf, N.K.2
Fischer, C.J.3
Lohman, T.M.4
-
44
-
-
0034635172
-
Demonstration of unidirectional single-stranded DNA translocation by PcrA helicase: Measurement of step size and translocation speed
-
Dillingham, M. S., Wigley, D. B. & Webb, M. R. Demonstration of unidirectional single-stranded DNA translocation by PcrA helicase: measurement of step size and translocation speed. Biochemistry 39, 205-212 (2000).
-
(2000)
Biochemistry
, vol.39
, pp. 205-212
-
-
Dillingham, M.S.1
Wigley, D.B.2
Webb, M.R.3
-
45
-
-
0037080166
-
Direct measurement of single-stranded DNA translocation by PcrA helicase using the fluorescent base analogue 2-aminopurine
-
Dillingham, M. S., Wigley, D. B. & Webb, M. R. Direct measurement of single-stranded DNA translocation by PcrA helicase using the fluorescent base analogue 2-aminopurine. Biochemistry 41, 643-651 (2002).
-
(2002)
Biochemistry
, vol.41
, pp. 643-651
-
-
Dillingham, M.S.1
Wigley, D.B.2
Webb, M.R.3
-
46
-
-
9244237061
-
ATP-dependent translocation of proteins along single-stranded DNA: Models and methods of analysis of pre-steady state kinetics
-
Fischer, C. J. & Lohman, T. M. ATP-dependent translocation of proteins along single-stranded DNA: models and methods of analysis of pre-steady state kinetics. J. Mol. Biol. 344, 1265-1286 (2004).
-
(2004)
J. Mol. Biol
, vol.344
, pp. 1265-1286
-
-
Fischer, C.J.1
Lohman, T.M.2
-
47
-
-
0035816217
-
-
Cheng, W., Hsieh, J., Brendza, K. M. & Lohman, T. M. E. coli Rep oligomers are required to initiate DNA unwinding in vitro. J. Mol. Biol. 310, 327-350 (2001).
-
Cheng, W., Hsieh, J., Brendza, K. M. & Lohman, T. M. E. coli Rep oligomers are required to initiate DNA unwinding in vitro. J. Mol. Biol. 310, 327-350 (2001).
-
-
-
-
48
-
-
22544482026
-
Autoinhibition of Escherichia coli Rep monomer helicase activity by its 2B subdomain
-
This paper demonstrates that Rep monomers can translocate along ssDNA and do not have helicase activity, but the deletion of subdomain 2B activates helicase activity of the monomer
-
Brendza, K. M. et al. Autoinhibition of Escherichia coli Rep monomer helicase activity by its 2B subdomain. Proc. Natl Acad. Sci. USA 102, 10076-10081 (2005). This paper demonstrates that Rep monomers can translocate along ssDNA and do not have helicase activity, but the deletion of subdomain 2B activates helicase activity of the monomer.
-
(2005)
Proc. Natl Acad. Sci. USA
, vol.102
, pp. 10076-10081
-
-
Brendza, K.M.1
-
49
-
-
0037474547
-
A dimer of Escherichia coli UvrD is the active form of the helicase in vitro
-
Pre-steady-state kinetic studies show that UvrD monomers do not possess helicase activity in vitro and that dimers are the minimal oligomeric form needed
-
Maluf, N. K., Fischer, C. J. & Lohman, T. M. A dimer of Escherichia coli UvrD is the active form of the helicase in vitro. J. Mol. Biol. 325, 913-935 (2003). Pre-steady-state kinetic studies show that UvrD monomers do not possess helicase activity in vitro and that dimers are the minimal oligomeric form needed.
-
(2003)
J. Mol. Biol
, vol.325
, pp. 913-935
-
-
Maluf, N.K.1
Fischer, C.J.2
Lohman, T.M.3
-
50
-
-
9244235535
-
Mechanism of ATP-dependent translocation of E.coli UvrD monomers along single-stranded DNA
-
Single round methods for studying ssDNA-translocase kinetics show that UvrD monomers are rapid and processive ssDNA translocases, but not helicases in vitro
-
Fischer, C. J., Maluf, N. K. & Lohman, T. M. Mechanism of ATP-dependent translocation of E.coli UvrD monomers along single-stranded DNA. J. Mol. Biol. 344, 1287-1309 (2004). Single round methods for studying ssDNA-translocase kinetics show that UvrD monomers are rapid and processive ssDNA translocases, but not helicases in vitro.
-
(2004)
J. Mol. Biol
, vol.344
, pp. 1287-1309
-
-
Fischer, C.J.1
Maluf, N.K.2
Lohman, T.M.3
-
51
-
-
0037069390
-
-
Nanduri, B., Byrd, A. K., Eoff, R. L., Tackett, A. J. & Raney, K. D. Pre-steady-state DNA unwinding by bacteriophage T4 Dda helicase reveals a monomeric molecular motor. Proc. Natl Acad. Sci. USA 99, 14722-14727 (2002). Pre-steady-state kinetic methods demonstrate that the T4 Dda monomer has limited helicase activity in vitro.
-
Nanduri, B., Byrd, A. K., Eoff, R. L., Tackett, A. J. & Raney, K. D. Pre-steady-state DNA unwinding by bacteriophage T4 Dda helicase reveals a monomeric molecular motor. Proc. Natl Acad. Sci. USA 99, 14722-14727 (2002). Pre-steady-state kinetic methods demonstrate that the T4 Dda monomer has limited helicase activity in vitro.
-
-
-
-
52
-
-
33845980606
-
DNA unwinding by Escherichia coli DNA helicase I (TraI) provides evidence for a processive monomeric molecular motor
-
Pre-steady-state kinetic methods demonstrate that the TraI monomer is a highly processive helicase in vitro
-
Sikora, B., Eoff, R. L., Matson, S. W. & Raney, K. D. DNA unwinding by Escherichia coli DNA helicase I (TraI) provides evidence for a processive monomeric molecular motor. J. Biol. Chem. 281, 36110-36116 (2006). Pre-steady-state kinetic methods demonstrate that the TraI monomer is a highly processive helicase in vitro.
-
(2006)
J. Biol. Chem
, vol.281
, pp. 36110-36116
-
-
Sikora, B.1
Eoff, R.L.2
Matson, S.W.3
Raney, K.D.4
-
53
-
-
3342896727
-
Periodic cycles of RNA unwinding and pausing by hepatitis C virus NS3 helicase
-
Serebrov, V. & Pyle, A. M. Periodic cycles of RNA unwinding and pausing by hepatitis C virus NS3 helicase. Nature 430, 476-480 (2004).
-
(2004)
Nature
, vol.430
, pp. 476-480
-
-
Serebrov, V.1
Pyle, A.M.2
-
54
-
-
2542468381
-
The functional interaction of the hepatitis C virus helicase molecules is responsible for unwinding processivity
-
Levin, M. K., Wang, Y. H. & Patel, S. S. The functional interaction of the hepatitis C virus helicase molecules is responsible for unwinding processivity. J. Biol. Chem. 279, 26005-26012 (2004).
-
(2004)
J. Biol. Chem
, vol.279
, pp. 26005-26012
-
-
Levin, M.K.1
Wang, Y.H.2
Patel, S.S.3
-
55
-
-
0037057630
-
Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase
-
Ha, T. et al. Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase. Nature 419, 638-641 (2002).
-
(2002)
Nature
, vol.419
, pp. 638-641
-
-
Ha, T.1
-
56
-
-
27644514163
-
Repetitive shuttling of a motor protein on DNA
-
Myong, S., Rasnik, I., Joo, C., Lohman, T. M. & Ha, T. Repetitive shuttling of a motor protein on DNA. Nature 437, 1321-1325 (2005).
-
(2005)
Nature
, vol.437
, pp. 1321-1325
-
-
Myong, S.1
Rasnik, I.2
Joo, C.3
Lohman, T.M.4
Ha, T.5
-
57
-
-
34547595373
-
Spring-loaded mechanism of DNA unwinding by hepatitis C virus NS3 helicase
-
A single molecule fluorescence study concluding that the NS3 helicase unwinds DNA with a mechanical step size of ∼3 bp, composed of 1 bp sub-steps
-
Myong, S., Bruno, M. M., Pyle, A. M. & Ha, T. Spring-loaded mechanism of DNA unwinding by hepatitis C virus NS3 helicase. Science 317, 513-516 (2007). A single molecule fluorescence study concluding that the NS3 helicase unwinds DNA with a mechanical step size of ∼3 bp, composed of 1 bp sub-steps.
-
(2007)
Science
, vol.317
, pp. 513-516
-
-
Myong, S.1
Bruno, M.M.2
Pyle, A.M.3
Ha, T.4
-
58
-
-
2342598305
-
Single-molecule assay reveals strand switching and enhanced processivity of UvrD
-
Dessinges, M. N., Lionnet, T., Xi, X. G., Bensimon, D. & Croquette, V. Single-molecule assay reveals strand switching and enhanced processivity of UvrD. Proc. Natl Acad. Sci. USA 101, 6439-6444 (2004).
-
(2004)
Proc. Natl Acad. Sci. USA
, vol.101
, pp. 6439-6444
-
-
Dessinges, M.N.1
Lionnet, T.2
Xi, X.G.3
Bensimon, D.4
Croquette, V.5
-
59
-
-
1542375262
-
Forward and reverse motion of single RecBCD molecules on DNA
-
Perkins, T. T., Li, H. W., Dalal, R. V., Gelles, J. & Block, S. M. Forward and reverse motion of single RecBCD molecules on DNA. Biophys. J. 86, 1640-1648 (2004).
-
(2004)
Biophys. J
, vol.86
, pp. 1640-1648
-
-
Perkins, T.T.1
Li, H.W.2
Dalal, R.V.3
Gelles, J.4
Block, S.M.5
-
60
-
-
0035905687
-
Processive translocation and DNA unwinding by individual RecBCD enzyme molecules
-
Bianco, P. R. et al. Processive translocation and DNA unwinding by individual RecBCD enzyme molecules. Nature 409, 374-378 (2001).
-
(2001)
Nature
, vol.409
, pp. 374-378
-
-
Bianco, P.R.1
-
61
-
-
34250766751
-
Single-molecule studies reveal dynamics of DNA unwinding by the ring-shaped T7 helicase
-
Johnson, D. S., Bai, L., Smith, B. Y., Patel, S. S. & Wang, M. D. Single-molecule studies reveal dynamics of DNA unwinding by the ring-shaped T7 helicase. Cell 129, 1299-1309 (2007).
-
(2007)
Cell
, vol.129
, pp. 1299-1309
-
-
Johnson, D.S.1
Bai, L.2
Smith, B.Y.3
Patel, S.S.4
Wang, M.D.5
-
62
-
-
30144436268
-
RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP
-
A single molecule, optical tweezers study concluding that the NS3 helicase unwinds RNA with a mechanical step size of ∼11 bp, composed of 3 bp sub-steps
-
Dumont, S. et al. RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP. Nature 439, 105-108 (2006). A single molecule, optical tweezers study concluding that the NS3 helicase unwinds RNA with a mechanical step size of ∼11 bp, composed of 3 bp sub-steps.
-
(2006)
Nature
, vol.439
, pp. 105-108
-
-
Dumont, S.1
-
63
-
-
35348856329
-
-
Cheng, W., Dumont, S., Tinoco, I. Jr & Bustamante, C. NS3 helicase actively separates RNA strands and senses sequence barriers ahead of the opening fork. Proc. Natl Acad. Sci. USA. 104, 13954-13959 (2007).
-
Cheng, W., Dumont, S., Tinoco, I. Jr & Bustamante, C. NS3 helicase actively separates RNA strands and senses sequence barriers ahead of the opening fork. Proc. Natl Acad. Sci. USA. 104, 13954-13959 (2007).
-
-
-
-
64
-
-
0042818314
-
The Escherichia coli RecQ helicase functions as a monomer
-
Xu, H. Q. et al. The Escherichia coli RecQ helicase functions as a monomer. J. Biol. Chem. 278, 34925-34933 (2003).
-
(2003)
J. Biol. Chem
, vol.278
, pp. 34925-34933
-
-
Xu, H.Q.1
-
65
-
-
34848840105
-
Bacillus stearothermophilus PcrA monomer is a single-stranded DNA translocase but not a processive helicase in vitro
-
Niedziela-Majka, A., Chesnik, M. A., Tomko, E. J. & Lohman, T. M. Bacillus stearothermophilus PcrA monomer is a single-stranded DNA translocase but not a processive helicase in vitro. J. Biol. Chem. 282, 27076-27085 (2007).
-
(2007)
J. Biol. Chem
, vol.282
, pp. 27076-27085
-
-
Niedziela-Majka, A.1
Chesnik, M.A.2
Tomko, E.J.3
Lohman, T.M.4
-
66
-
-
0033527761
-
An oligomeric form of the E. coli UvrD helicase is required for optimal helicase activity
-
Ali, J. A., Maluf, N. K. & Lohman, T. M. An oligomeric form of the E. coli UvrD helicase is required for optimal helicase activity. J. Mol. Biol. 293, 815-833 (1999).
-
(1999)
J. Mol. Biol
, vol.293
, pp. 815-833
-
-
Ali, J.A.1
Maluf, N.K.2
Lohman, T.M.3
-
67
-
-
0041355282
-
Kinetic mechanism for formation of the active, dimeric UvrD helicase-DNA complex
-
Maluf, N. K., Ali, J. A. & Lohman, T. M. Kinetic mechanism for formation of the active, dimeric UvrD helicase-DNA complex. J. Biol. Chem. 278, 31930-31940 (2003).
-
(2003)
J. Biol. Chem
, vol.278
, pp. 31930-31940
-
-
Maluf, N.K.1
Ali, J.A.2
Lohman, T.M.3
-
68
-
-
34447266733
-
Directional loading and stimulation of PcrA helicase by the replication initiator protein RepD
-
The RepD protein is shown to greatly stimulate the helicase activity of the PcrA enzyme in vitro
-
Zhang, W. et al. Directional loading and stimulation of PcrA helicase by the replication initiator protein RepD. J. Mol. Biol. 371, 336-348 (2007). The RepD protein is shown to greatly stimulate the helicase activity of the PcrA enzyme in vitro.
-
(2007)
J. Mol. Biol
, vol.371
, pp. 336-348
-
-
Zhang, W.1
-
69
-
-
41849106909
-
Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding
-
Yang, Y. et al. Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding. Nucleic Acids Res. 36, 1976-1989 (2008).
-
(2008)
Nucleic Acids Res
, vol.36
, pp. 1976-1989
-
-
Yang, Y.1
-
70
-
-
25444456899
-
Increasing the length of the single-stranded overhang enhances unwinding of duplex DNA by bacteriophage T4 Dda helicase
-
Byrd, A. K. & Raney, K. D. Increasing the length of the single-stranded overhang enhances unwinding of duplex DNA by bacteriophage T4 Dda helicase. Biochemistry 44, 12990-12997 (2005).
-
(2005)
Biochemistry
, vol.44
, pp. 12990-12997
-
-
Byrd, A.K.1
Raney, K.D.2
-
71
-
-
15444371665
-
Multiple full-length NS3 molecules are required for optimal unwinding of oligonucleotide DNA in vitro
-
Tackett, A. J., Chen, Y., Cameron, C. E. & Raney, K. D. Multiple full-length NS3 molecules are required for optimal unwinding of oligonucleotide DNA in vitro. J. Biol. Chem. 280, 10797-10806 (2005).
-
(2005)
J. Biol. Chem
, vol.280
, pp. 10797-10806
-
-
Tackett, A.J.1
Chen, Y.2
Cameron, C.E.3
Raney, K.D.4
-
72
-
-
0027518667
-
Helicase-catalyzed DNA unwinding
-
Lohman, T. M. Helicase-catalyzed DNA unwinding. J. Biol. Chem. 268, 2269-2272 (1993).
-
(1993)
J. Biol. Chem
, vol.268
, pp. 2269-2272
-
-
Lohman, T.M.1
-
73
-
-
0029893874
-
Mechanisms of helicase-catalyzed DNA unwinding
-
Lohman, T. M. & Bjornson, K. P. Mechanisms of helicase-catalyzed DNA unwinding. Ann. Rev. Biochem. 65, 169-214 (1996).
-
(1996)
Ann. Rev. Biochem
, vol.65
, pp. 169-214
-
-
Lohman, T.M.1
Bjornson, K.P.2
-
75
-
-
0021071027
-
The gene 4 protein of bacteriophate T7. Characterization of helicase activity
-
Matson, S. W., Tabor, S. & Richardson, C. C. The gene 4 protein of bacteriophate T7. Characterization of helicase activity. J. Biol. Chem. 258, 14017-14024 (1983).
-
(1983)
J. Biol. Chem
, vol.258
, pp. 14017-14024
-
-
Matson, S.W.1
Tabor, S.2
Richardson, C.C.3
-
76
-
-
34247571461
-
Kinesin motor mechanics: Binding, stepping, tracking, gating, and limping
-
Block, S. M. Kinesin motor mechanics: binding, stepping, tracking, gating, and limping. Biophys. J. 92, 2986-2995 (2007).
-
(2007)
Biophys. J
, vol.92
, pp. 2986-2995
-
-
Block, S.M.1
-
77
-
-
2542475076
-
Effects of temperature and ATP on the kinetic mechanism and kinetic step-size for E. coli RecBCD helicase-catalyzed DNA unwinding
-
Lucius, A. L. & Lohman, T. M. Effects of temperature and ATP on the kinetic mechanism and kinetic step-size for E. coli RecBCD helicase-catalyzed DNA unwinding. J. Mol. Biol. 339, 751-771 (2004).
-
(2004)
J. Mol. Biol
, vol.339
, pp. 751-771
-
-
Lucius, A.L.1
Lohman, T.M.2
-
78
-
-
0025051352
-
Differential ATP requirements distinguish the DNA translocation and DNA unwinding activities of the Escherichia coli PRI A protein
-
Lee, M. S. & Marians, K. J. Differential ATP requirements distinguish the DNA translocation and DNA unwinding activities of the Escherichia coli PRI A protein. J. Biol. Chem. 265, 17078-17083 (1990).
-
(1990)
J. Biol. Chem
, vol.265
, pp. 17078-17083
-
-
Lee, M.S.1
Marians, K.J.2
-
79
-
-
34247602963
-
-
Tomko, E. J., Fischer, C. J., Niedziela-Majka, A. & Lohman, T. M. A nonuniform stepping mechanism for E. coli UvrD monomer translocation along single-stranded DNA. Mol. Cell 26, 335-347 (2007). Pre-steady-state measurements of both ssDNA-translocation rates and ATP hydrolysis shows an ATP coupling stoichiometry of ∼1 ATP hydrolyzed per base translocated with a slow kinetic step occurring every 4-5 bases.
-
Tomko, E. J., Fischer, C. J., Niedziela-Majka, A. & Lohman, T. M. A nonuniform stepping mechanism for E. coli UvrD monomer translocation along single-stranded DNA. Mol. Cell 26, 335-347 (2007). Pre-steady-state measurements of both ssDNA-translocation rates and ATP hydrolysis shows an ATP coupling stoichiometry of ∼1 ATP hydrolyzed per base translocated with a slow kinetic step occurring every 4-5 bases.
-
-
-
-
80
-
-
0019835096
-
Theoretical aspects of translocation on DNA: Adenosine triphosphatase and treadmilling binding proteins
-
Hill, T. L. & Tsuchiya, T. Theoretical aspects of translocation on DNA: Adenosine triphosphatase and treadmilling binding proteins. Proc. Natl Acad. Sci. USA 78, 4796-4800 (1981).
-
(1981)
Proc. Natl Acad. Sci. USA
, vol.78
, pp. 4796-4800
-
-
Hill, T.L.1
Tsuchiya, T.2
-
81
-
-
0035150184
-
Unwinding the 'Gordian knot' of helicase action
-
Soultanas, P. & Wigley, D. B. Unwinding the 'Gordian knot' of helicase action. Trends Biochem. Sci. 26, 47-54 (2001).
-
(2001)
Trends Biochem. Sci
, vol.26
, pp. 47-54
-
-
Soultanas, P.1
Wigley, D.B.2
-
82
-
-
33748335314
-
Structure-based model of the stepping motor of PcrA helicase
-
Yu, J., Ha, T. & Schulten, K. Structure-based model of the stepping motor of PcrA helicase. Biophys. J. 91, 2097-2114 (2006).
-
(2006)
Biophys. J
, vol.91
, pp. 2097-2114
-
-
Yu, J.1
Ha, T.2
Schulten, K.3
-
83
-
-
0026546001
-
Allosteric effects of nucleotide cofactors on Escherichia coli Rep helicase-DNA binding
-
Wong, I. & Lohman, T. M. Allosteric effects of nucleotide cofactors on Escherichia coli Rep helicase-DNA binding. Science 256, 350-355 (1992).
-
(1992)
Science
, vol.256
, pp. 350-355
-
-
Wong, I.1
Lohman, T.M.2
-
84
-
-
34247634331
-
-
eds Tamanoi, F. & Hackney, D. D, Academic Press, New York
-
Lohman, T. M. et al. DNA helicases, motors that move along nucleic acids: lessons from the SF1 helicase superfamily (eds Tamanoi, F. & Hackney, D. D.) 303-369 (Academic Press, New York, 2003).
-
(2003)
DNA helicases, motors that move along nucleic acids: Lessons from the SF1 helicase superfamily
, pp. 303-369
-
-
Lohman, T.M.1
-
85
-
-
0038607629
-
ATP binding modulates the nucleic acid affinity of hepatitis C virus helicase
-
Levin, M. K., Gurjar, M. M. & Patel, S. S. ATP binding modulates the nucleic acid affinity of hepatitis C virus helicase. J. Biol. Chem. 278, 23311-23316 (2003).
-
(2003)
J. Biol. Chem
, vol.278
, pp. 23311-23316
-
-
Levin, M.K.1
Gurjar, M.M.2
Patel, S.S.3
-
86
-
-
20444440763
-
A Brownian motor mechanism of translocation and strand separation by hepatitis C virus helicase
-
Levin, M. K., Gurjar, M. & Patel, S. S. A Brownian motor mechanism of translocation and strand separation by hepatitis C virus helicase. Nature Struct. Mol. Biol. 12, 429-435 (2005).
-
(2005)
Nature Struct. Mol. Biol
, vol.12
, pp. 429-435
-
-
Levin, M.K.1
Gurjar, M.2
Patel, S.S.3
-
87
-
-
0026597376
-
-
Lohman, T. M. E. coli DNA helicases: mechanisms of DNA unwinding. Molecular Microbiology 6, 5-14 (1992).
-
Lohman, T. M. E. coli DNA helicases: mechanisms of DNA unwinding. Molecular Microbiology 6, 5-14 (1992).
-
-
-
-
88
-
-
0027172508
-
Escherichia coli Rep helicase unwinds DNA by an active mechanism
-
Amaratunga, M. & Lohman, T. M. Escherichia coli Rep helicase unwinds DNA by an active mechanism. Biochemistry 32, 6815-6820 (1993).
-
(1993)
Biochemistry
, vol.32
, pp. 6815-6820
-
-
Amaratunga, M.1
Lohman, T.M.2
-
89
-
-
41349092240
-
Opening of nucleic-acid double strands by helicases: Active versus passive opening
-
Betterton, M. D. & Julicher, F. Opening of nucleic-acid double strands by helicases: active versus passive opening. Phys. Rev. E Stat. Nonlin. Soft Matter Phys. 71, 1-11 (2005).
-
(2005)
Phys. Rev. E Stat. Nonlin. Soft Matter Phys
, vol.71
, pp. 1-11
-
-
Betterton, M.D.1
Julicher, F.2
-
90
-
-
0035951425
-
A general model for nucleic acid helicases and their "coupling" within macromolecular machines
-
von Hippel, P. H. & Delagoutte, E. A general model for nucleic acid helicases and their "coupling" within macromolecular machines. Cell 104, 177-190 (2001).
-
(2001)
Cell
, vol.104
, pp. 177-190
-
-
von Hippel, P.H.1
Delagoutte, E.2
-
91
-
-
42449124020
-
-
Lohman, T. M. DNA helicases: dimeric enzyme action (ed. Lennarz, W. a. L., M. D.) 618-624 (Academic Press, Elsevier Science (USA), San Diego, California, 2004).
-
Lohman, T. M. DNA helicases: dimeric enzyme action (ed. Lennarz, W. a. L., M. D.) 618-624 (Academic Press, Elsevier Science (USA), San Diego, California, 2004).
-
-
-
-
92
-
-
0034679815
-
Uncoupling DNA translocation and helicase activity in PcrA: Direct evidence for an active mechanism
-
Soultanas, P., Dillingham, M. S., Wiley, P., Webb, M. R. & Wigley, D. B. Uncoupling DNA translocation and helicase activity in PcrA: direct evidence for an active mechanism. EMBO J. 19, 3799-3810 (2000).
-
(2000)
EMBO J
, vol.19
, pp. 3799-3810
-
-
Soultanas, P.1
Dillingham, M.S.2
Wiley, P.3
Webb, M.R.4
Wigley, D.B.5
-
93
-
-
0036447339
-
DNA unwinding step-size of E. coli RecBCD helicase determined from single turnover chemical quenched-flow kinetic studies
-
Lucius, A. L. et al. DNA unwinding step-size of E. coli RecBCD helicase determined from single turnover chemical quenched-flow kinetic studies. J. Mol. Biol. 324, 409-428 (2002).
-
(2002)
J. Mol. Biol
, vol.324
, pp. 409-428
-
-
Lucius, A.L.1
-
94
-
-
33744951869
-
Escherichia coli RecQ is a rapid, efficient, and monomeric helicase
-
Zhang, X. D. et al. Escherichia coli RecQ is a rapid, efficient, and monomeric helicase. J. Biol. Chem. 281, 12655-12663 (2006).
-
(2006)
J. Biol. Chem
, vol.281
, pp. 12655-12663
-
-
Zhang, X.D.1
-
95
-
-
33644793170
-
Intermediates revealed in the kinetic mechanism for DNA unwinding by a monomeric helicase
-
Eoff, R. L. & Raney, K. D. Intermediates revealed in the kinetic mechanism for DNA unwinding by a monomeric helicase. Nature Struct. Mol. Biol. 13, 242-249 (2006).
-
(2006)
Nature Struct. Mol. Biol
, vol.13
, pp. 242-249
-
-
Eoff, R.L.1
Raney, K.D.2
-
96
-
-
0026673415
-
Processivity of the DNA helicase activity of Escherichia coli recBCD enzyme
-
Roman, L. J., Eggleston, A. K. & Kowalczykowski, S. C. Processivity of the DNA helicase activity of Escherichia coli recBCD enzyme. J. Biol. Chem. 267, 4207-4214 (1992).
-
(1992)
J. Biol. Chem
, vol.267
, pp. 4207-4214
-
-
Roman, L.J.1
Eggleston, A.K.2
Kowalczykowski, S.C.3
-
97
-
-
0037590607
-
RecBCD enzyme is a bipolar helicase
-
Dillingham, M. S., Spies, M., Kowalczykowski, S. C. RecBCD enzyme is a bipolar helicase. Nature 423, 893-897 (2003).
-
(2003)
Nature
, vol.423
, pp. 893-897
-
-
Dillingham, M.S.1
Spies, M.2
Kowalczykowski, S.C.3
-
98
-
-
0037698985
-
RecBCD enzyme is a DNA helicase with fast and slow motors of opposite polarity
-
Taylor, A. F. & Smith, G. R. RecBCD enzyme is a DNA helicase with fast and slow motors of opposite polarity. Nature 423, 889-893 (2003).
-
(2003)
Nature
, vol.423
, pp. 889-893
-
-
Taylor, A.F.1
Smith, G.R.2
-
99
-
-
2542430217
-
Fluorescence stopped-flow studies of single turnover kinetics of E. coli RecBCD helicase-catalyzed DNA unwinding
-
Lucius, A. L., Jason Wong, C. & Lohman, T. M. Fluorescence stopped-flow studies of single turnover kinetics of E. coli RecBCD helicase-catalyzed DNA unwinding. J. Mol. Biol. 339, 731-750 (2004).
-
(2004)
J. Mol. Biol
, vol.339
, pp. 731-750
-
-
Lucius, A.L.1
Jason Wong, C.2
Lohman, T.M.3
-
100
-
-
0024512057
-
Characterization of the helicase activity of the Escherichia coli RecBCD enzyme using a novel helicase assay
-
Roman, L. J. & Kowalczykowski, S. C. Characterization of the helicase activity of the Escherichia coli RecBCD enzyme using a novel helicase assay. Biochemistry 28, 2863-2873 (1989).
-
(1989)
Biochemistry
, vol.28
, pp. 2863-2873
-
-
Roman, L.J.1
Kowalczykowski, S.C.2
-
101
-
-
0034682407
-
Translocation step size and mechanism of the RecBC DNA helicase
-
Bianco, P. R. & Kowalczykowski, S. C. Translocation step size and mechanism of the RecBC DNA helicase. Nature 405, 368-372 (2000).
-
(2000)
Nature
, vol.405
, pp. 368-372
-
-
Bianco, P.R.1
Kowalczykowski, S.C.2
-
102
-
-
0031563796
-
The RecBCD enzyme initiation complex for DNA unwinding: Enzyme positioning and DNA opening
-
Farah, J. A. & Smith, G. R. The RecBCD enzyme initiation complex for DNA unwinding: enzyme positioning and DNA opening. J. Mol. Biol. 272, 699-715 (1997).
-
(1997)
J. Mol. Biol
, vol.272
, pp. 699-715
-
-
Farah, J.A.1
Smith, G.R.2
-
103
-
-
24644435846
-
Energetics of DNA end binding by E. coli RecBC and RecBCD helicases indicate loop formation in the 3′-single-stranded DNA tail
-
Wong, C. J., Lucius, A. L. & Lohman, T. M. Energetics of DNA end binding by E. coli RecBC and RecBCD helicases indicate loop formation in the 3′-single-stranded DNA tail. J. Mol. Biol. 352, 765-782 (2005).
-
(2005)
J. Mol. Biol
, vol.352
, pp. 765-782
-
-
Wong, C.J.1
Lucius, A.L.2
Lohman, T.M.3
-
104
-
-
0034625236
-
Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides
-
Singleton, M. R., Sawaya, M. R., Ellenberger, T. & Wigley, D. B. Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides. Cell 101, 589-600 (2000).
-
(2000)
Cell
, vol.101
, pp. 589-600
-
-
Singleton, M.R.1
Sawaya, M.R.2
Ellenberger, T.3
Wigley, D.B.4
-
105
-
-
0033617191
-
Escherichia coli DNA helicase II is active as a monomer
-
Mechanic, L. E., Hall, M. C. & Matson, S. W. Escherichia coli DNA helicase II is active as a monomer. J. Biol. Chem. 274, 12488-12498 (1999).
-
(1999)
J. Biol. Chem
, vol.274
, pp. 12488-12498
-
-
Mechanic, L.E.1
Hall, M.C.2
Matson, S.W.3
-
106
-
-
0037058917
-
The 2B domain of the Escherichia coli Rep protein is not required for DNA helicase activity
-
Cheng, W. et al. The 2B domain of the Escherichia coli Rep protein is not required for DNA helicase activity. Proc. Natl Acad. Sci. USA 99, 16006-16011 (2002).
-
(2002)
Proc. Natl Acad. Sci. USA
, vol.99
, pp. 16006-16011
-
-
Cheng, W.1
-
107
-
-
33749173326
-
The Escherichia coli UvrD helicase is essential for Tus removal during recombination-dependent replication restart from Ter sites
-
Bidnenko, V., Lestini, R. & Michel, B. The Escherichia coli UvrD helicase is essential for Tus removal during recombination-dependent replication restart from Ter sites. Mol. Microbiol. 62, 382-396 (2006).
-
(2006)
Mol. Microbiol
, vol.62
, pp. 382-396
-
-
Bidnenko, V.1
Lestini, R.2
Michel, B.3
-
108
-
-
8844265345
-
The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants
-
983-988
-
Flores, M. J., Bidnenko, V. & Michel, B. The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants. EMBO Rep. 5, 983-988 (2004).
-
(2004)
EMBO Rep
, vol.5
-
-
Flores, M.J.1
Bidnenko, V.2
Michel, B.3
-
109
-
-
0037673941
-
DNA helicase Srs2 disrupts the Rad51 presynaptic filament
-
Krejci, L. et al. DNA helicase Srs2 disrupts the Rad51 presynaptic filament. Nature 423, 305-309 (2003).
-
(2003)
Nature
, vol.423
, pp. 305-309
-
-
Krejci, L.1
-
110
-
-
2542453681
-
Role of ATP hydrolysis in the antirecombinase function of Saccharomyces cerevisiae Srs2 protein
-
Krejci, L. et al. Role of ATP hydrolysis in the antirecombinase function of Saccharomyces cerevisiae Srs2 protein. J. Biol. Chem. 279, 23193-23199 (2004).
-
(2004)
J. Biol. Chem
, vol.279
, pp. 23193-23199
-
-
Krejci, L.1
-
111
-
-
27744445335
-
The yeast Pif1p helicase removes telomerase from telomeric DNA
-
Boule, J. B., Vega, L. R. & Zakian, V. A. The yeast Pif1p helicase removes telomerase from telomeric DNA. Nature 438, 57-61 (2005).
-
(2005)
Nature
, vol.438
, pp. 57-61
-
-
Boule, J.B.1
Vega, L.R.2
Zakian, V.A.3
-
112
-
-
34250305132
-
DNA helicase activity of PcrA is not required for the displacement of RecA protein from DNA or inhibition of RecA-mediated strand exchange
-
Anand, S. P., Zheng, H., Bianco, P. R., Leuba, S. H. & Khan, S. A. DNA helicase activity of PcrA is not required for the displacement of RecA protein from DNA or inhibition of RecA-mediated strand exchange. J. Bacteriol. 189, 4502-4509 (2007).
-
(2007)
J. Bacteriol
, vol.189
, pp. 4502-4509
-
-
Anand, S.P.1
Zheng, H.2
Bianco, P.R.3
Leuba, S.H.4
Khan, S.A.5
-
113
-
-
34247859353
-
Controlling the motor activity of a transcription-repair coupling factor: Autoinhibition and the role of RNA polymerase
-
Smith, A. J., Szczelkun, M. D. & Savery, N. J. Controlling the motor activity of a transcription-repair coupling factor: autoinhibition and the role of RNA polymerase. Nucleic Acids Res. 35, 1802-1811 (2007).
-
(2007)
Nucleic Acids Res
, vol.35
, pp. 1802-1811
-
-
Smith, A.J.1
Szczelkun, M.D.2
Savery, N.J.3
-
114
-
-
41949094586
-
Structure of the DNA repair helicase HEL308 reveals DNA binding and autoinhibitory domains
-
Richards, J. D. et al. Structure of the DNA repair helicase HEL308 reveals DNA binding and autoinhibitory domains. J. Biol. Chem. 283, 5118-5126 (2008).
-
(2008)
J. Biol. Chem
, vol.283
, pp. 5118-5126
-
-
Richards, J.D.1
-
115
-
-
36849075152
-
The serine protease domain of hepatitis C viral NS3 activates RNA helicase activity by promoting the binding of RNA substrate
-
Beran, R. K. F., Serebrov, V. & Pyle, A. M. The serine protease domain of hepatitis C viral NS3 activates RNA helicase activity by promoting the binding of RNA substrate. J. Biol. Chem. 282, 34913-34920 (2007).
-
(2007)
J. Biol. Chem
, vol.282
, pp. 34913-34920
-
-
Beran, R.K.F.1
Serebrov, V.2
Pyle, A.M.3
-
116
-
-
0017669060
-
φX174 cistron A protein is a multifunctional enzyme in DNA replication
-
Eisenberg, S., Griffith, J. D. & Kornberg, A. φX174 cistron A protein is a multifunctional enzyme in DNA replication. Proc. Natl Acad. Sci. USA 74, 3198-3202 (1977).
-
(1977)
Proc. Natl Acad. Sci. USA
, vol.74
, pp. 3198-3202
-
-
Eisenberg, S.1
Griffith, J.D.2
Kornberg, A.3
-
117
-
-
0026646152
-
The RecB subunit of the Escherichia coli RecBCD enzyme couples ATP hydrolysis to DNA unwinding
-
Boehmer, P. E. & Emmerson, P. T. The RecB subunit of the Escherichia coli RecBCD enzyme couples ATP hydrolysis to DNA unwinding. J. Biol. Chem. 267, 4981-4987 (1992).
-
(1992)
J. Biol. Chem
, vol.267
, pp. 4981-4987
-
-
Boehmer, P.E.1
Emmerson, P.T.2
-
118
-
-
23944518444
-
An inactivated nuclease-like domain in RecC with novel function: Implications for evolution
-
Rigden, D. J. An inactivated nuclease-like domain in RecC with novel function: implications for evolution. BMC Struct. Biol. 5, 9 (2005).
-
(2005)
BMC Struct. Biol
, vol.5
, pp. 9
-
-
Rigden, D.J.1
-
119
-
-
0036500976
-
The hepatitis C viral NS3 protein is a processive DNA helicase with cofactor enhanced RNA unwinding
-
Pang, P. S., Jankowsky, E., Planet., P. J. & Pyle, A. M. The hepatitis C viral NS3 protein is a processive DNA helicase with cofactor enhanced RNA unwinding. EMBO J. 21, 1168-1176 (2002).
-
(2002)
EMBO J
, vol.21
, pp. 1168-1176
-
-
Pang, P.S.1
Jankowsky, E.2
Planet, P.J.3
Pyle, A.M.4
-
120
-
-
36049052525
-
RecBCD enzyme switches lead motor subunits in response to chi recognition
-
Single molecule studies showing that recognition of the 'Chi' sequence within the unwound DNA results in a switching of the lead motor during unwinding with a dramatic effect on the rates and nuclease activity of this bipolar helicase
-
Spies, M., Amitani, I., Baskin, R. J. & Kowalczykowski, S. C. RecBCD enzyme switches lead motor subunits in response to chi recognition. Cell 131, 694-705 (2007). Single molecule studies showing that recognition of the 'Chi' sequence within the unwound DNA results in a switching of the lead motor during unwinding with a dramatic effect on the rates and nuclease activity of this bipolar helicase.
-
(2007)
Cell
, vol.131
, pp. 694-705
-
-
Spies, M.1
Amitani, I.2
Baskin, R.J.3
Kowalczykowski, S.C.4
-
121
-
-
0029879214
-
A helicase assay based on the displacement of fluorescent, nucleic acid-binding ligands
-
Eggleston, A. K., Rahim, N. A. & Kowalczykowski, S. C. A helicase assay based on the displacement of fluorescent, nucleic acid-binding ligands. Nucleic Acids Res. 24, 1179-1186 (1996).
-
(1996)
Nucleic Acids Res
, vol.24
, pp. 1179-1186
-
-
Eggleston, A.K.1
Rahim, N.A.2
Kowalczykowski, S.C.3
-
122
-
-
0023660955
-
Interaction between the replication origin and the initiator protein of the filamentous phage f1
-
Greenstein, D. & Horiuchi, K. Interaction between the replication origin and the initiator protein of the filamentous phage f1. J. Mol. Biol. 197, 157-174 (1987).
-
(1987)
J. Mol. Biol
, vol.197
, pp. 157-174
-
-
Greenstein, D.1
Horiuchi, K.2
-
123
-
-
0026054936
-
DNA-induced dimerization of the Escherichia coli rep helicase
-
Chao, K. & Lohman, T. M. DNA-induced dimerization of the Escherichia coli rep helicase. J. Mol. Biol. 221, 1165-1181 (1991).
-
(1991)
J. Mol. Biol
, vol.221
, pp. 1165-1181
-
-
Chao, K.1
Lohman, T.M.2
-
124
-
-
33749120512
-
The UvrD helicase and its modulation by the mismatch repair protein MutL
-
Matson, S. W. & Robertson, A. B. The UvrD helicase and its modulation by the mismatch repair protein MutL. Nucleic Acids Res. 34, 4089-4097 (2006).
-
(2006)
Nucleic Acids Res
, vol.34
, pp. 4089-4097
-
-
Matson, S.W.1
Robertson, A.B.2
-
125
-
-
34447515950
-
Mycobacterial UvrD1 is a Ku-dependent DNA helicase that plays a role in multiple DNA repair events, including double-strand break repair
-
Sinha, K. M., Stephanou, N. C., Gao, F., Glickman, M. S. & Shuman, S. Mycobacterial UvrD1 is a Ku-dependent DNA helicase that plays a role in multiple DNA repair events, including double-strand break repair. J. Biol. Chem. 282, 15114-15125 (2007).
-
(2007)
J. Biol. Chem
, vol.282
, pp. 15114-15125
-
-
Sinha, K.M.1
Stephanou, N.C.2
Gao, F.3
Glickman, M.S.4
Shuman, S.5
-
126
-
-
34547121734
-
A central role for SSB in Escherichia coli RecQ DNA helicase function
-
Shereda, R. D., Bernstein, D. A. & Keck, J. L. A central role for SSB in Escherichia coli RecQ DNA helicase function. J. Biol. Chem. 282, 19247-19258 (2007).
-
(2007)
J. Biol. Chem
, vol.282
, pp. 19247-19258
-
-
Shereda, R.D.1
Bernstein, D.A.2
Keck, J.L.3
-
127
-
-
13444282478
-
PriA helicase and SSB interact physically and functionally
-
Cadman, C. J. & McGlynn, P. PriA helicase and SSB interact physically and functionally. Nucleic Acids Res. 32, 6378-6387 (2004).
-
(2004)
Nucleic Acids Res
, vol.32
, pp. 6378-6387
-
-
Cadman, C.J.1
McGlynn, P.2
|