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Volumn 304, Issue 5671, 2004, Pages 730-734

Protein Displacement by DExH/D "RNA Helicases" Without Duplex Unwinding

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; METABOLISM; PHYSIOLOGY; RNA; SEPARATION; SUBSTRATES;

EID: 2342443461     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1095596     Document Type: Article
Times cited : (205)

References (32)
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  • 14
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    • note
    • Removal of all RNA that protrudes from the EJC does not compromise the stability of the EJC (Fig. 3, B and F), indicating that the stability of the EJC-RNA complex does not depend on any RNA secondary structure surrounding the EJC.
  • 18
    • 2342432698 scopus 로고    scopus 로고
    • note
    • Materials and methods are available on Science Online.
  • 20
    • 2342435446 scopus 로고    scopus 로고
    • data not shown
    • M. E. Fairman et al., data not shown.
    • Fairman, M.E.1
  • 21
    • 2342430227 scopus 로고    scopus 로고
    • note
    • TRAP-RNA complexes were formed before the reaction for 10 min. NPH-II was added, and incubation was continued for at least 5 min. Remodeling reactions were started by adding a mixture of ATP and RNA scavenger, which consists of DNA oligonucleotides that hybridize to the TRAP-binding site and thus prevent rebinding of TRAP once it has been displaced. Further materials and methods are available (18).
  • 31
    • 2342444292 scopus 로고    scopus 로고
    • note
    • NPH-II forms, in an ATP-dependent manner, a tight complex with the RNA from which TRAP has been displaced. This complex could not be disrupted without destruction of the TRAP-RNA complex The NPH-II-RNA complex co-migrated with the TRAP-RNA complex under all separation conditions tested.
  • 32
    • 2342436072 scopus 로고    scopus 로고
    • note
    • We thank P. Linder for kindly providing the DED1 plasmid and S. Shuman for recombinant baculovirus containing the NPH-II expression vector. HeLa Cells were obtained from the National Cell Culture Center. We are grateful to Q. Yang for help with the initial characterization of DED1 and to P. deHaseth and M. Caprara for comments on the manuscript. This work was supported by a grant from NIH to E.J. and by grants from NIH to T.W.N. P.G. was supported by grants from NIH and NSF, and M.E.F. was supported by an NIH training grant.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.