Processive tanslocation and DNA unwinding by individual RecBCD enzyme molecules

Nature

Volumn 409, Issue 6818, 2001, Pages 374-378

  Bianco, Piero R ^a,b   Brewer, Laurence R ^e   Corzett, Michele ^c   Balhorn, Rod ^b   Yeh, Yin ^d   Kowalczykowski, Stephen C ^a,b   Baskin, Ronald J ^b  

^a Departments of Pathology   (United States)

^b Molecular and Cellular Biology   (United States)

^c LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e NONE

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DOUBLE STRANDED DNA; HELICASE; ADENOSINE TRIPHOSPHATE; EXODEOXYRIBONUCLEASE; EXODEOXYRIBONUCLEASE V; VIRUS DNA;

ARTICLE; BASE PAIRING; BIOTINYLATION; CHEMICAL REACTION; CHROMOSOME TRANSLOCATION; DNA DETERMINATION; FLUORESCENCE MICROSCOPY; PRIORITY JOURNAL; STEADY STATE; IMAGE PROCESSING; LASER; METABOLISM; MICROSCOPY; OPTICS; TRANSPORT AT THE CELLULAR LEVEL;

ADENOSINE TRIPHOSPHATE; BIOLOGICAL TRANSPORT; DNA; DNA HELICASES; DNA, VIRAL; EXODEOXYRIBONUCLEASE V; EXODEOXYRIBONUCLEASES; IMAGE PROCESSING, COMPUTER-ASSISTED; LASERS; MICROSCOPY, FLUORESCENCE; MICROSCOPY, VIDEO; OPTICS;

EID: 0035905687     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35053131     Document Type: Article

References (19)

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5. Protamine-induced condensation and decondensation of the same DNA molecule
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8. Single-molecule manipulation of double-stranded DNA using optical tweezers: Interaction studies of DNA with RecA and YOYO-1
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10. Characterization of the helicase activity of the Escherichia coli RecBCD enzyme using a novel helicase assay
11. 2109 CD enzyme, has helicase activity but does not promote efficient joint molecule formation in vitro
12. Unwinding and rewinding of DNA by the recBC enzyme
13. Differences in the chemical reactivity of individual molecules of an enzyme
14. Single-molecule studies of the effect of template tension on T7 DNA polymerase activity
15. Characterization of the adenosinetriphosphatase activity of the Escherichia coli RecBCD enzyme: Relationship of ATP hydrolysis to the unwinding of duplex DNA
16. Step size measurements on the translocation mechanism of the RecBC DNA helicase
17. Binding of double-stranded DNA by Escherichia coli RecA protein monitored by a fluorescent dye displacement assay
18. SSB protein controls RecBCD enzyme nuclease activity during unwinding: A new role for looped intermediates