Evaluating the 3C-like protease activity of SARS-Coronavirus: Recommendations for standardized assays for drug discovery

Virus Research

Volumn 133, Issue 1, 2008, Pages 63-73

  Grum Tokars, Valerie ^a   Ratia, Kiira ^a   Begaye, Adrian ^a   Baker, Susan C ^b   Mesecar, Andrew D ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b LOYOLA UNIVERSITY CHICAGO   (United States)

Author keywords

3C like protease; Coronavirus; FRET based assays; SARS CoV; Severe acute respiratory syndrome; Viral protease inhibitors

Indexed keywords

3C LIKE PROTEASE; DIMER; DITHIOTHREITOL; EDETIC ACID; PEPTIDE; PROTEINASE INHIBITOR; REAGENT; SODIUM CHLORIDE; SUCCIMER; UNCLASSIFIED DRUG; VIRUS ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; ARTIFACT; CARBOXY TERMINAL SEQUENCE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISSOCIATION CONSTANT; DRUG DETERMINATION; DRUG RESEARCH; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME SUBSTRATE; EQUILIBRIUM CONSTANT; EVALUATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SARS CORONAVIRUS; SENSITIVITY ANALYSIS; STANDARD; VIRUS REPLICATION;

CORONAVIRUS; SARS CORONAVIRUS;

EID: 40649104356     PISSN: 01681702     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.virusres.2007.02.015     Document Type: Article

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