Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra α-helical domain

EMBO Journal

Volumn 21, Issue 13, 2002, Pages 3213-3224

  Anand, Kanchan ^a   Palm, Gottfried J ^a   Mesters, Jeroen R ^a   Siddell, Stuart G ^a   Ziebuhr, John ^a   Hilgenfeld, Rolf ^a  

^a FRITZ LIPMANN INSTITUTE   (Germany)

Author keywords

3C like; Catalytic dyad; Coronavirus; Proteinase; X ray crystallography

Indexed keywords

CHYMOTRYPSIN; CYSTEINE; GLUTAMINE; HISTIDINE; LEUCINE; PROTEINASE; UNCLASSIFIED DRUG; VIRAL MAIN PROTEINASE; VIRUS ENZYME;

ARTICLE; CORONAVIRUS; CRYSTAL STRUCTURE; DIMERIZATION; ENZYME ACTIVE SITE; ENZYME STRUCTURE; ENZYME SUBSTRATE; GASTROENTERITIS; MOLECULAR MODEL; MOLECULAR STABILITY; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OPTICAL RESOLUTION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN INTERACTION; PROTEIN PROCESSING; SEQUENCE HOMOLOGY; VIRUS MORPHOLOGY;

AMINO ACID SEQUENCE; BINDING SITES; CHYMOTRYPSIN; CRYSTALLOGRAPHY, X-RAY; CYSTEINE ENDOPEPTIDASES; DIMERIZATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; TRANSMISSIBLE GASTROENTERITIS VIRUS;

CORONAVIRUS; RNA VIRUSES; TRANSMISSIBLE GASTROENTERITIS VIRUS;

EID: 0036646055     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdf327     Document Type: Article

References (61)

1. Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases
2. Poliovirus RNA synthesis utilizes an RNP complex formed around the 5′-end of viral RNA
3. A fast algorithm for rendering space-filling molecule pictures
4. Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: Structural and functional implications
5. The refined crystal structure of the 3C gene product from hepatitis A virus: Specific proteinase activity and RNA recognition
6. Free R value: A novel statistical quantity for assessing the accuracy of crystal structures
7. Crystallography and NMR system: A new software suite for macromolecular structure determination
8. Nidovirales: A new order comprising Coronaviridae and Arteriviridae
9. The CCP4 suite: Programs for protein crystallography
10. Phase combination and cross validation in iterated density-modification calculations
11. Equine arteritis virus is not a togavirus but belongs to the coronaviruslike superfamily
12. Complete sequence (20 kilobases) of the polyprotein-encoding gene 1 of transmissible gastroenteritis virus
13. Molecular basis of transmissible gastroenteritis virus epidemiology
14. Refined structure of α-lytic protease at 1.7 Å resolution
15. Crystal and molecular structures of the complex of α-chymotrypsin with its inhibitor turkey ovomucoid third domain at 1.8 Å resolution
16. Motif-based searching in TOPS protein topology databases
17. Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. A distinct protein superfamily with a common structural fold
18. Coronavirus genome: Prediction of putative functional domains in the non-structural polyprotein by comparative amino acid sequence analysis
19. A minimal principle in the phase problem
20. Conservation of substrate specificities among coronavirus main proteases
21. Mutational analysis of the active centre of coronavirus 3C-like proteases
22. Seleno- methionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): A vehicle for direct determination of three-dimensional structure
23. Protein structure comparison by alignment of distance matrices
24. Structure of product and inhibitor complexes of Streptomyces griseus protease A at 1.8 Å resolution: A model for serine protease catalysis
25. Improved methods for building protein models in electron density maps and the location of errors in these models
26. Structure of papain refined at 1.65 Å resolution
27. MOLSCRIPT - A program to produce both detailed and schematic plots of protein structures
28. PROCHECK: A program to check the stereochemical quality of protein structures
29. The interpretation of protein structures: Estimation of static accessibility
30. pro) binds specifically to the 5′-noncoding region of the viral RNA
31. Characterisation and mutational analysis of an ORF 1a-encoding proteinase domain responsible for proteolytic processing of the infectious bronchitis virus 1a/1b polyprotein
32. Intracellular and in vitro-translated 27-kDa proteins contain the 3C-like proteinase activity of the coronavirus MHV-A59
33. Determinants of mouse hepatitis virus 3C-like proteinase activity
34. Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59
35. The picornaviral 3C proteinases: Cysteine nucleophiles in serine proteinase folds
36. Solvent content of protein crystals
37. Structure of human rhinovirus 3C protease reveals a trypsin-like polypeptide fold, RNA-binding site, and means for cleaving precursor polyprotein
38. Structure-assisted design of mechanism-based irreversible inhibitors of human rhinovirus 3C protease with potent antiviral activity against multiple rhinovirus serotypes
39. Refined x-ray crystallographic structure of the poliovirus 3C gene product
40. Further characterization of the coronavirus infectious bronchitis virus 3C-like proteinase and determination of a new cleavage site
41. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons
42. Processing of x-ray diffraction data collected in oscillation mode
43. Proteolytic processing of picornaviral polyprotein
44. Complete genome sequence of transmissible gastroenteritis coronavirus PUR46-MAD clone and evolution of the purdue virus cluster
45. The structure of the 2A proteinase from a common cold virus: A proteinase responsible for the shut-off of host-cell protein synthesis
46. Mercaptide-imidazolium ion-pair: The reactive nucleophile in papain catalysis
47. Transmissible gastroenteritis virus
48. Processing of the coronavirus MHV-JHM polymerase polyprotein: Identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a
49. Expression and characterization of a recombinant murine coronavirus 3C-like proteinase
50. The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools
51. Structure of α-chymotrypsin refined at 1.68 Å resolution
52. SFCHECK: A unified set of procedures for evaluating the quality of macromolecular structure-factor data and their agreement with the atomic model
53. WHAT IF: A molecular modeling and drug design program
54. The design and implementation of SnB version 2.0
55. On the use of merging R-factor as a quality indicator for x-ray data
56. Interaction between the 5′-terminal cloverleaf and 3AB/3CDpro of poliovirus is essential for RNA replication
57. Site-directed mutagenesis of herpesvirus glycoprotein phosphorylation sites by recombination polymerase chain reaction
58. Processing of the human coronavirus 229E replicase polyproteins by the virus-encoded 3C-like proteinase: Identification of proteolytic products and cleavage sites common to pp1a and pp1ab
59. Characterization of a human coronavirus (strain 229E) 3C-like proteinase activity
60. Biosynthesis, purification, and characterization cf the human coronavirus 229E 3C-like proteinase
61. Virus-encoded proteinases, and proteolytic processing in the Nidovirales