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The first report that multiple CDC25B variants derive from a single mRNA by alternative splicing events
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Lindqvist, A., Kallstrom, H., Lundgren, A., Barsoum, E. & Rosenthal, C. K. Cdc25B cooperates with Cdc25A to induce mitosis but has a unique role in activating cyclin B1-Cdk1 at the centrosome. J. Cell Biol. 171, 35-45 (2005). An important study exploring the relative roles of CDC25A and CDC25B in inducing mitotic onset. The authors show that CDC25B is required for the initial activation of CDK1-cyclin B at the centrosomes, whereas CDC25A is involved in chromatin condensation.
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Boutros, R., Dozier, C. & Ducommun, B. The when and wheres of CDC25 phosphatases. Curr. Opin. Cell Biol. 18, 185-191 (2006). A detailed review of the complex mechanisms that regulate CDC25 activities throughout the cell cycle.
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24
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p55 cdc25 is a nuclear phosphoprotein required for the initiation of mitosis in human cells
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Gabrielli, B. G. et al. Cytoplasmic accumulation of CDC25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells. J. Cell Sci. 109, 1081-1093 (1996). The first demonstration of a link between cytoplasmic CDC25B accumulation and centrosomal events that occur during the G2-M transition.
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27
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Hyperphosphorylation of the N-terminal domain of cdc25 regulates activity toward cyclinB1/cdc2 but not cyclin A/cdk2
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Ferguson, A. M., White, L. S., Donovan, P. J. & Piwnica-Worms, H. Normal cell cycle and checkpoint responses in mice and cells lacking Cdc25B and Cdc25C protein phosphatases. Mol. Cell Biol. 25, 2853-2860 (2005). An important study in which the authors challenge the idea that all three CDC25 isoforms are required for normal cell-cycle progression, by successfully generating mice that lack both CDC25B and CDC25C without any obvious defects in cell-cycle function or checkpoint responses.
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32
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-/- female mice are sterile because of permanent meiotic arrest, resulting from lack of maturation promoting factor (MPF; CDK1-cyclinB1 complex) activity.
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Dutertre, S. et al. Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2/M transition. J. Cell Sci. 117, 2523-2531 (2004). This study demonstrates that phosphorylation by Aurora A is required for CDC25B activity and localization to the centrosomes to initiate mitotic entry.
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Dutertre, S. et al. Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2/M transition. J. Cell Sci. 117, 2523-2531 (2004). This study demonstrates that phosphorylation by Aurora A is required for CDC25B activity and localization to the centrosomes to initiate mitotic entry.
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Mils, V. et al. Specific interaction between 14. 3. 3 isoforms and the human CDC25B phosphatase. Oncogene 19, 1257-1265 (2000).
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Kramer, A. et al. Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase. Nature Cell Biol. 6, 884-891 (2004). An interesting study in which CHK1 is reported to localize to the centrosomes of interphase cells, and this pool of CHK1 functions to prevent the premature activation of CDK1-cyclin B.
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Kramer, A. et al. Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase. Nature Cell Biol. 6, 884-891 (2004). An interesting study in which CHK1 is reported to localize to the centrosomes of interphase cells, and this pool of CHK1 functions to prevent the premature activation of CDK1-cyclin B.
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51
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33751160547
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Schmitt, E. et al. CHK1 phosphorylates CDC25B during the cell cycle in the absence of DNA damage. J. Cell Sci. 119, 4269-4275 (2006). This study shows that CHK1 phosphorylates CDC25B in vitro and in vivo during interphase in the absence of DNA damage, in order to inhibit its mitosis-inducing activity.
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