Cytoplasmic accumulation of cdc25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells

Journal of Cell Science

Volumn 109, Issue 5, 1996, Pages 1081-1093

  Gabrielli, Brian G ^a   De Souza, Colin P C ^a   Tonks, Ian D ^a   Clark, Joanne M ^a   Hayward, Nicholas K ^a   Ellem, Kay A O ^a  

^a QIMR BERGHOFER MEDICAL RESEARCH INSTITUTE   (Australia)

Author keywords

cdc25; Microtubule; Mitosis

Indexed keywords

CYCLIN B; CYCLIN DEPENDENT KINASE; MUTANT PROTEIN; PHOSPHATASE;

ARTICLE; CELL CYCLE; CELL NUCLEUS; CENTROSOME; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; ENZYME LOCALIZATION; HELA CELL; HUMAN; HUMAN CELL; INTERPHASE; MICROTUBULE; MITOSIS; MITOSIS SPINDLE; PRIORITY JOURNAL; PROPHASE; PROTEIN TRANSPORT;

ANTIBODIES, MONOCLONAL; BASE SEQUENCE; CDC25 PHOSPHATASE; CELL CYCLE PROTEINS; CENTROSOME; CYTOPLASM; G2 PHASE; GENES, DOMINANT; HELA CELLS; HUMANS; METAPHASE; MICROTUBULES; MITOTIC SPINDLE APPARATUS; MOLECULAR SEQUENCE DATA; PHOSPHOPROTEIN PHOSPHATASE; PROPHASE;

EID: 0029937532     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (49)

1. Alpha, C. E., Ducommun, B., Beach, D. and Hyams, J. S. (1990). Distinct nuclear and spindle pole body populations of cyclin-cdc2 in fission yeast. Nature 347, 680-682.
2. cdc2 inhibitory kinases. Mol. Biol. Cell 5, 989-1001.
3. 2/M and enters vesicles at anaphase. EMBO J. 8, 3985-3995.
4. Bailly, E., Pines, J., Hunter, T. and Bornens, M. (1992). Cytoplasmic accumulation of cyclin Bl in human cells: association with a detergent-resistent compartment within the centrosome. J. Cell Sci. 101, 529-545.
5. cdc2 protein kinase. Mol. Cell Biol. 10, 3607-3618.
6. Chambers, T. C. and Langan, T. A. (1990). Purification and characterisation of growth-associated H1 histone kinase from Novikoff hepatoma cells. J. Biol. Chem. 265, 16940-16947.
7. Cohen, G. M., Sun, X.-M., Snowden, R. T., Ormerod, M. G. and Dinsdale, D. (1993). Identification of a transitional preapoptotic population of thymocytes. J. Immunol. 151, 566-574.
8. Deng, W. P. and Nickoloff, J. A. (1992). Site directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200, 81-88.
9. Ducommun, B., Draetta, G., Young, P. and Beach, D. (1990). Fission yeast cdc25 is a cell-cycle regulated protein. Biochem. Biophys. Res. Commun. 167, 301-309.
10. Dunphy, W. G. and Kumagai, A. (1991). The cdc25 protein contains an intrinsic phosphatase activity. Cell 67, 189-196.
11. wee1 mitotic inhibitor is a tyrosine/serine kinase. Nature 349, 808-811.
12. Feiler, H. S. and Jacobs, T. W. (1990). Cell division in higher plants: a cdc2 gene, its 34-kDa product, and histone H1 activity in pea. Proc. Nat. Acad. Sci. USA 87, 5397-5401.
13. Frangioni, J. V. and Neel, B. G. (1993). Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Anal. Biochem. 210, 179-187.
14. Gabrielli, B. G., Lee, M. S., Walker, D. H., Piwnica-Worms, H. and Maller, J. L. (1992). cdc25 regulates the phosphorylation and activity of the Xenopus cdk2 protein kinase complex. J. Biol. Chem. 267, 18040-18046.
15. Galaktionov, K. and Beach, D. (1991). Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic kinases. Cell 67, 1181-1194.
16. Gallant, P. and Nigg, E. A. (1992). Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells. J. Cell Biol. 117, 213-224.
17. cdc2. Cell 67, 197-211.
18. cdc2 kinase is restricted to mitosis in the mammalian cdc2 mutant FT210. J. Cell Biol. 117, 1041-1053.
19. Heald, R., McLoughlin, M. and McKeon, F. (1993). Human wee 1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated cdc2 kinase. Cell 74, 463-474.
20. 1/S transistion. EMBO J. 13, 4302-4310.
21. Jinno, S., Suto, K., Nagata, A., Igarashi, M., Kanaota, Y., Nojima, H. and Okayama, H. (1994). cdc25A is a novel phosphatase functioning early in the cell cycle. EMBO J. 13, 1549-1556.
22. Kochanski, R. S. and Borisy, G. G. (1990). Mode of centriole duplication and distribution. J. Cell Biol. 110, 1599-1605.
23. 2/M transistions of the cell cycle: identification of major phosphorylation sites. EMBO J. 10, 3331-3341.
24. cdc2 kinase activation in vertebrates. EMBO J. 10, 3331-3341.
25. Kuriyama, R. and Borisy, G. G. (1981). Microtubule-nucleating activity of centrosomes in CHO cells is independent of the centriole cycle, but coupled to the mitotic cycle. J. Cell Biol. 91, 822-826.
26. cdc2 kinase induces marked changes in cell shape, cytoskeletal organisation, and chromatin structure in mammalian fibroblasts. Cell 60, 151-165.
27. +. Nature 327, 31-35.
28. cdc2. Mol. Biol. Cell 3, 73-84.
29. Masuda, H., Sevik, M. and Cande, W. Z. (1992). In vitro microtubule-nucleating activity of spindle pole bodies in fission yeast Schizosaccharomyces pombe: cell-cycle dependent activation in Xenopus cell-free extracts. J. Cell Biol. 117, 1055-1066.
30. cdc25 is a nuclear protein required for the initiation of mitosis in human cells. Proc. Nat. Acad. Sci. USA 88, 10500-10504.
31. cdc25 mitotic inducer in fission yeast. Nature 344, 549-552.
32. + gene occurs in humans and is highly expressed in some cancer cells. New Biol. 3, 959-968.
33. cdc2 protein kinase in vertebrates. EMBO J. 10, 3321-3329.
34. 2 for control of mitosis in fission yeast. Nature 292, 558-560.
35. Ohta, K., Shiina, N., Okumura, E., Hisanaga, S., Kishimoto, T., Endo, S., Gotoh, G., Nishida, E. and Sakai, H. (1993). Microtubule nucleating activity of centrosomes in cell-free extracts from Xenopus eggs: involvement of phosphorylation and accumulation of pericentriolar material. J. Cell Sci. 104, 125-137.
36. + dependent manner. EMBO J. 10, 1255-1263.
37. Peter, M., Nakagawa, J., Doree, M., Labbe, J. C and Nigg, E. A. (1990). Identification of major nucleolar proteins as candidate substrates of cdc2 kinase. Cell 61, 591-602.
38. Pines. J. and Hunter, T. (1991). Human cyclins A and B1 are differentially located in the cell and undergo cell-cycle dependent nuclear transport. J. Cell Biol 115, 1-17.
39. Riabowol, K., Draetta, G., Brizuela, L., Vandre, D. and Beach, D. (1989). The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells. Cell 57, 393-401.
40. + functions as an inducer in the mitotic control of fission yeast. Cell 45, 145-153.
41. 2. Proc. Nat. Acad. Sci. USA 87, 5139-5143.
42. Sebastian, B., Kakizuka, A. and Hunter, T. (1993). cdc25M2 activation of cyclin-dependent kinases by dephosphorylation of threonine-14 and tyrosine-15. Proc. Nat. Acad. Sci. USA 90, 3521-3524.
43. Seki, T., Yamashita, K., Nishitani, H., Takagi, T., Russell, P. and Nishimoto, T. (1992). Chromosome condensation caused by loss of RCC1 function requires the cdc25C protein that is located in the cytoplasm. Mol. Biol. Cell 3, 1373-1388.
44. cdc2/cyclin B complex in vitro by human cdc25 protein. Nature 351, 242-245.
45. Sun, X.-M., Snowden, R. T., Skilleter, D. N., Dinsdale, D., Ormerod, M. G. and Cohen, G. M. (1992). A flow-cytometric method for the separation and quantitation of normal and apoptotic thymocytes. Anal. Biochem. 204, 242-245.
46. Takebe, Y., Seiki, M., Fujisawa, J., Hoy, P., Yokota, K., Arai, K., Yoshida, M. and Arai, N. (1988). SRα promotor: an efficient and versitile mammalian cDNA expression system composed of the simian virus 40 early promotor and the R-U5 segment of human T-cell Leukemia virus Type 1 long terminal repeat. Mol. Cell Biol. 8, 466-472.
47. van den Heuval, S. and Harlow, E. (1993). Distinct roles for cyclin dependent kinases in cell cycle control. Science 262, 2050-2054.
48. Verde, F., Labbe, J., Dorre, M. and Karsenti, E. (1990). Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs. Nature 343, 233-238.
49. Verde, F., Dogterom, M., Stelzer, E., Karsenti, E. and Leibler, S. (1992). Control of microtubule dynamics and length by cyclin A and cyclin B-dependent kinases in Xenopus egg extracts. J. Cell Biol. 118, 1097-1108.