-
1
-
-
0029964835
-
A novel method for real time quantitative RT-PCR
-
Gibson, U.E., Heid, C.A. & Williams, P.M. A novel method for real time quantitative RT-PCR. Genome Res. 6, 995-1001 (1996).
-
(1996)
Genome Res
, vol.6
, pp. 995-1001
-
-
Gibson, U.E.1
Heid, C.A.2
Williams, P.M.3
-
2
-
-
0033775615
-
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
-
Bustin, S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25, 169-193 (2000).
-
(2000)
J. Mol. Endocrinol
, vol.25
, pp. 169-193
-
-
Bustin, S.A.1
-
3
-
-
0036278859
-
Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream
-
Ginzinger, D.G. Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream. Exp. Hematol. 30, 503-512 (2002).
-
(2002)
Exp. Hematol
, vol.30
, pp. 503-512
-
-
Ginzinger, D.G.1
-
4
-
-
14044265394
-
Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
-
Bustin, S.A. & Nolan, T. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Tech. 15, 155-66 (2004).
-
(2004)
J. Biomol. Tech
, vol.15
, pp. 155-166
-
-
Bustin, S.A.1
Nolan, T.2
-
5
-
-
1542328972
-
Properties of the reverse transcription reaction in mRNA quantification
-
Stahlberg, A., Hakansson, J., Xian, X., Semb, H. & Kubista, M. Properties of the reverse transcription reaction in mRNA quantification. Clin. Chem. 50, 509-515 (2004).
-
(2004)
Clin. Chem
, vol.50
, pp. 509-515
-
-
Stahlberg, A.1
Hakansson, J.2
Xian, X.3
Semb, H.4
Kubista, M.5
-
6
-
-
4344564046
-
Comparison of reverse transcriptases in gene expression analysis
-
Stahlberg, A., Kubista, M. & Pfaffl, M. Comparison of reverse transcriptases in gene expression analysis. Clin. Chem. 50, 1678-1680 (2004).
-
(2004)
Clin. Chem
, vol.50
, pp. 1678-1680
-
-
Stahlberg, A.1
Kubista, M.2
Pfaffl, M.3
-
7
-
-
0036668567
-
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems
-
Bustin, S.A. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems. J. Mol. Endocrinol. 29, 23-39 (2002).
-
(2002)
J. Mol. Endocrinol
, vol.29
, pp. 23-39
-
-
Bustin, S.A.1
-
8
-
-
26844512816
-
Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis
-
Bustin, S.A. & Mueller, R. Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. Clin. Sci. (Lond.) 109, 365-379 (2005).
-
(2005)
Clin. Sci. (Lond.)
, vol.109
, pp. 365-379
-
-
Bustin, S.A.1
Mueller, R.2
-
9
-
-
33644917430
-
Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer
-
Bustin, S.A. & Mueller, R. Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer. Mol. Aspects Med. 27, 192-223 (2006).
-
(2006)
Mol. Aspects Med
, vol.27
, pp. 192-223
-
-
Bustin, S.A.1
Mueller, R.2
-
10
-
-
14744306436
-
Simultaneous amplification and detection of specific DNA sequences
-
Higuchi, R., Dollinger, G., Walsh, P.S. & Griffith, R. Simultaneous amplification and detection of specific DNA sequences. Nat. Biotechnol. 10, 413-417 (1992).
-
(1992)
Nat. Biotechnol
, vol.10
, pp. 413-417
-
-
Higuchi, R.1
Dollinger, G.2
Walsh, P.S.3
Griffith, R.4
-
11
-
-
15744381713
-
Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions
-
Higuchi, R., Fockler, C., Dollinger, G. & Watson, R. Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions. Biotechnol. (NY) 11, 1026-1030 (1993).
-
(1993)
Biotechnol. (NY)
, vol.11
, pp. 1026-1030
-
-
Higuchi, R.1
Fockler, C.2
Dollinger, G.3
Watson, R.4
-
12
-
-
22044458051
-
Real-time, fluorescence-based quantitative PCR: A snapshot of current procedures and preferences
-
Bustin, S.A. Real-time, fluorescence-based quantitative PCR: A snapshot of current procedures and preferences. Expert Rev. Mol. Diagn. 5, 493-498 (2005).
-
(2005)
Expert Rev. Mol. Diagn
, vol.5
, pp. 493-498
-
-
Bustin, S.A.1
-
13
-
-
21344458602
-
Quantitative real-time RT-PCR-a perspective
-
Bustin, S.A., Benes, V., Nolan, T. & Pfaffl, M.W. Quantitative real-time RT-PCR-a perspective. J. Mol. Endocrinol. 34, 597-601 (2005).
-
(2005)
J. Mol. Endocrinol
, vol.34
, pp. 597-601
-
-
Bustin, S.A.1
Benes, V.2
Nolan, T.3
Pfaffl, M.W.4
-
15
-
-
33644975004
-
RNA integrity and the effect on the real-time qRT-PCR performance
-
Fleige, S. & Pfaffl, M.W. RNA integrity and the effect on the real-time qRT-PCR performance. Mol. Aspects Med. 27, 126-139 (2006).
-
(2006)
Mol. Aspects Med
, vol.27
, pp. 126-139
-
-
Fleige, S.1
Pfaffl, M.W.2
-
16
-
-
33645241407
-
The RIN: An RNA integrity number for assigning integrity values to RNA measurements
-
Schroeder, A. et al. The RIN: An RNA integrity number for assigning integrity values to RNA measurements. BMC Mol. Biol. 7, 3 (2006).
-
(2006)
BMC Mol. Biol
, vol.7
, pp. 3
-
-
Schroeder, A.1
-
17
-
-
0345257783
-
Chipping away at the chip bias: RNA degradation in microarray analysis
-
Auer, H. et al. Chipping away at the chip bias: RNA degradation in microarray analysis. Nat. Genet. 35, 292-293 (2003).
-
(2003)
Nat. Genet
, vol.35
, pp. 292-293
-
-
Auer, H.1
-
18
-
-
4344599509
-
Pre-PCR processing: Strategies to generate PCR-compatible samples
-
Radstrom, P., Knutsson, R., Wolffs, P., Lovenklev, M. & Lofstrom, C. Pre-PCR processing: Strategies to generate PCR-compatible samples. Mol. Biotechnol. 26, 133-146 (2004).
-
(2004)
Mol. Biotechnol
, vol.26
, pp. 133-146
-
-
Radstrom, P.1
Knutsson, R.2
Wolffs, P.3
Lovenklev, M.4
Lofstrom, C.5
-
19
-
-
10744228596
-
Prevalence of selective inhibition of HPV-16 DNA amplification in cervicovaginal lavages
-
Lefevre, J., Hankins, C., Pourreaux, K., Voyer, H. & Coutlee, F. Prevalence of selective inhibition of HPV-16 DNA amplification in cervicovaginal lavages. J. Med. Virol. 72, 132-137 (2004).
-
(2004)
J. Med. Virol
, vol.72
, pp. 132-137
-
-
Lefevre, J.1
Hankins, C.2
Pourreaux, K.3
Voyer, H.4
Coutlee, F.5
-
20
-
-
1242338153
-
Comparison of two methods for the detection of hepatitis A virus in clam samples (Tapes spp.) by reverse transcription-nested PCR
-
Sunen, E., Casas, N., Moreno, B. & Zigorraga, C. Comparison of two methods for the detection of hepatitis A virus in clam samples (Tapes spp.) by reverse transcription-nested PCR. Int. J. Food Microbiol. 91, 147-154 (2004).
-
(2004)
Int. J. Food Microbiol
, vol.91
, pp. 147-154
-
-
Sunen, E.1
Casas, N.2
Moreno, B.3
Zigorraga, C.4
-
21
-
-
1642497870
-
Removal of PCR inhibitors using dielectrophoresis as a selective filter in a microsystem
-
Perch-Nielsen, I.R., Bang, D.D., Poulsen, C.R., El-Ali, J. & Wolff, A. Removal of PCR inhibitors using dielectrophoresis as a selective filter in a microsystem. Lab Chip 3, 212-216 (2003).
-
(2003)
Lab Chip
, vol.3
, pp. 212-216
-
-
Perch-Nielsen, I.R.1
Bang, D.D.2
Poulsen, C.R.3
El-Ali, J.4
Wolff, A.5
-
22
-
-
15444364560
-
Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors
-
Jiang, J., Alderisio, K.A., Singh, A. & Xiao, L. Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors. Appl. Environ. Microbiol. 71, 1135-1141 (2005).
-
(2005)
Appl. Environ. Microbiol
, vol.71
, pp. 1135-1141
-
-
Jiang, J.1
Alderisio, K.A.2
Singh, A.3
Xiao, L.4
-
23
-
-
0141482157
-
Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage
-
Guy, R.A., Payment, P., Krull, U.J. & Horgen, P.A. Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Appl. Environ. Microbiol. 69, 5178-5185 (2003).
-
(2003)
Appl. Environ. Microbiol
, vol.69
, pp. 5178-5185
-
-
Guy, R.A.1
Payment, P.2
Krull, U.J.3
Horgen, P.A.4
-
24
-
-
15444365256
-
Quantitative real-time PCR for cancer detection: The lymphoma case
-
Stahlberg, A., Zoric, N., Aman, P. &, Kubista, M. Quantitative real-time PCR for cancer detection: The lymphoma case. Expert Rev. Mol. Diagn. 5, 221-230 (2005).
-
(2005)
Expert Rev. Mol. Diagn
, vol.5
, pp. 221-230
-
-
Stahlberg, A.1
Zoric, N.2
Aman, P.3
Kubista, M.4
-
25
-
-
0037230778
-
Quantitative real-time PCR method for detection of B-lymphocyte monoclonality by comparison of kappa and lambda immunoglobulin light chain expression
-
Stahlberg, A., Aman, P., Ridell, B., Mostad, P. & Kubista, M. Quantitative real-time PCR method for detection of B-lymphocyte monoclonality by comparison of kappa and lambda immunoglobulin light chain expression. Clin. Chem. 49, 51-59 (2003).
-
(2003)
Clin. Chem
, vol.49
, pp. 51-59
-
-
Stahlberg, A.1
Aman, P.2
Ridell, B.3
Mostad, P.4
Kubista, M.5
-
26
-
-
1542356381
-
Standardized determination of real-time PCR efficiency from a single reaction set-up
-
Tichopad, A., Dilger, M., Schwarz, G. & Pfaffl, M.W. Standardized determination of real-time PCR efficiency from a single reaction set-up. Nucleic Acids Res. 31, e122 (2003).
-
(2003)
Nucleic Acids Res
, vol.31
-
-
Tichopad, A.1
Dilger, M.2
Schwarz, G.3
Pfaffl, M.W.4
-
27
-
-
0037435395
-
Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
-
Ramakers, C., Ruijter, J.M., Deprez, R.H. & Moorman, A.F. Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci. Lett. 339, 62-66 (2003).
-
(2003)
Neurosci. Lett
, vol.339
, pp. 62-66
-
-
Ramakers, C.1
Ruijter, J.M.2
Deprez, R.H.3
Moorman, A.F.4
-
28
-
-
0036289137
-
Validation of a quantitative method real time PCR kinetics
-
Liu, W. & Saint D.A. Validation of a quantitative method real time PCR kinetics. Biochem. Biophys. Res. Commun. 294, 347-353 (2002).
-
(2002)
Biochem. Biophys. Res. Commun
, vol.294
, pp. 347-353
-
-
Liu, W.1
Saint, D.A.2
-
29
-
-
0031784953
-
Armored RNA technology for production of ribonuclease-resistant viral RNA controls and standards
-
Pasloske, S.L., Walkerpeach, C.R., Obermoeller, R.D., Winkler, M. & DuBois, D.B. Armored RNA technology for production of ribonuclease-resistant viral RNA controls and standards. J. Clin. Microbiol. 36, 3590-3594 (1998).
-
(1998)
J. Clin. Microbiol
, vol.36
, pp. 3590-3594
-
-
Pasloske, S.L.1
Walkerpeach, C.R.2
Obermoeller, R.D.3
Winkler, M.4
DuBois, D.B.5
-
30
-
-
0038642772
-
Description of a multiplex Bordetella pertussis and Bordetella parapertussis LightCycler PCR assay with inhibition control
-
Cloud, J.L et al. Description of a multiplex Bordetella pertussis and Bordetella parapertussis LightCycler PCR assay with inhibition control. Diagn. Microbiol. Infect. Dis. 46, 189-195 (2003).
-
(2003)
Diagn. Microbiol. Infect. Dis
, vol.46
, pp. 189-195
-
-
Cloud, J.L.1
-
31
-
-
33645293441
-
SPUD: A qPCR assay for the detection of inhibitors in nucleic acid preparations
-
Nolan, T., Hands, R.E., Ogunkolade, B.W. & Bustin, S.A. SPUD: A qPCR assay for the detection of inhibitors in nucleic acid preparations. Anal. Biochem. 351, 308-310 (2006).
-
(2006)
Anal. Biochem
, vol.351
, pp. 308-310
-
-
Nolan, T.1
Hands, R.E.2
Ogunkolade, B.W.3
Bustin, S.A.4
-
32
-
-
0028170672
-
Comparison of M-MLV reverse transcriptase and Tth polymerase activity in RT-PCR of samples with low virus burden
-
Cusi, M.G., Valassina, M. & Valensin, P.E. Comparison of M-MLV reverse transcriptase and Tth polymerase activity in RT-PCR of samples with low virus burden. Biotechniques 17, 1034-1036 (1994).
-
(1994)
Biotechniques
, vol.17
, pp. 1034-1036
-
-
Cusi, M.G.1
Valassina, M.2
Valensin, P.E.3
-
33
-
-
0029940698
-
Sensitivity of tyrosinase mRNA detection-by RT-PCR: RTth DNA polymerase vs. MMLV-RT and AmpliTaq polymerase
-
Juhasz, A., Ravi, S. & O'Connell, C.D. Sensitivity of tyrosinase mRNA detection-by RT-PCR: RTth DNA polymerase vs. MMLV-RT and AmpliTaq polymerase. Biotechniques 20, 592-600 (1996).
-
(1996)
Biotechniques
, vol.20
, pp. 592-600
-
-
Juhasz, A.1
Ravi, S.2
O'Connell, C.D.3
-
34
-
-
0028598720
-
Evaluation of a 'one tube' reverse transcription-polymerase chain reaction for the detection of ruminant pestiviruses
-
Easton, L.A., Vilcek, S. & Nettleton, P.F. Evaluation of a 'one tube' reverse transcription-polymerase chain reaction for the detection of ruminant pestiviruses. J. Virol. Methods 50, 343-348 (1994).
-
(1994)
J. Virol. Methods
, vol.50
, pp. 343-348
-
-
Easton, L.A.1
Vilcek, S.2
Nettleton, P.F.3
-
35
-
-
0036534582
-
-
Vandesompele, J., De Paepe, A. & Speleman, F. Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR green I-real-time RT-PCR 303, 95-98 (2002).
-
Vandesompele, J., De Paepe, A. & Speleman, F. Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR green I-real-time RT-PCR 303, 95-98 (2002).
-
-
-
-
37
-
-
29144495882
-
PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
-
Suslov, O. & Steindler, D.A. PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency. Nucleic Acids Res 33, e181 (2005).
-
(2005)
Nucleic Acids Res
, vol.33
-
-
Suslov, O.1
Steindler, D.A.2
-
38
-
-
12344305007
-
Gestational profile of Na+/H+ exchanger and Cl/HCO3- anion exchanger mRNA expression in placenta using real-time QPCR
-
Lacey, H.A., Nolan, T., Greenwood, S.L., Glazier, J.D. & Sibley, C.P. Gestational profile of Na+/H+ exchanger and Cl/HCO3- anion exchanger mRNA expression in placenta using real-time QPCR. Placenta 26 93-98 (2005).
-
(2005)
Placenta
, vol.26
, pp. 93-98
-
-
Lacey, H.A.1
Nolan, T.2
Greenwood, S.L.3
Glazier, J.D.4
Sibley, C.P.5
-
39
-
-
33745482592
-
Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA
-
Stangegaard, M., Dufva, I.H. & Dufva, M. Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA. Biotechniques 40, 649-657 (2006).
-
(2006)
Biotechniques
, vol.40
, pp. 649-657
-
-
Stangegaard, M.1
Dufva, I.H.2
Dufva, M.3
-
40
-
-
34648863662
-
-
Lewis, F. & Maughan, N.J. Extraction of Total RNA from Formalin-Fixed Paraffin-Embedded Tissue. in A-Z of quantitative PCR (ed. Bustin, S.A.) (IUL, La Jolla, California, 2004).
-
Lewis, F. & Maughan, N.J. Extraction of Total RNA from Formalin-Fixed Paraffin-Embedded Tissue. in A-Z of quantitative PCR (ed. Bustin, S.A.) (IUL, La Jolla, California, 2004).
-
-
-
-
41
-
-
0036667911
-
Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions
-
Lekanne Deprez, R.H., Fijnvandraat, A.C., Ruijter, J.M. & Moorman, A.F. Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions. Anal. Biochem. 307, 63-69 (2002).
-
(2002)
Anal. Biochem
, vol.307
, pp. 63-69
-
-
Lekanne Deprez, R.H.1
Fijnvandraat, A.C.2
Ruijter, J.M.3
Moorman, A.F.4
-
42
-
-
29144484144
-
Multiplexed tandem PCR: Gene profiling from small amounts of RNA using SYBR Green detection
-
Stanley, K.K. & Szewczuk, E. Multiplexed tandem PCR: Gene profiling from small amounts of RNA using SYBR Green detection. Nucleic Acids Res 33, e180 (2005).
-
(2005)
Nucleic Acids Res
, vol.33
-
-
Stanley, K.K.1
Szewczuk, E.2
-
43
-
-
29244475713
-
Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability
-
Hilscher, C., Vahrson, W. & Dittmer, D.P. Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability. Nucleic Acids Res. 33, e182 (2005).
-
(2005)
Nucleic Acids Res
, vol.33
-
-
Hilscher, C.1
Vahrson, W.2
Dittmer, D.P.3
-
44
-
-
13244265562
-
Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell
-
Hartshorn, C., Anshelevich, A. & Wangh, L.J. Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell. BMC Biotechnol 5, 2 (2005).
-
(2005)
BMC Biotechnol
, vol.5
, pp. 2
-
-
Hartshorn, C.1
Anshelevich, A.2
Wangh, L.J.3
-
45
-
-
0037248812
-
RTPrimerDB: The real-time PCR primer and probe database
-
Pattyn, F., Speleman, F., De Paepe, A. & Vandesompele, J. RTPrimerDB: the real-time PCR primer and probe database. Nucleic Acids Res. 31, 122-123 (2003).
-
(2003)
Nucleic Acids Res
, vol.31
, pp. 122-123
-
-
Pattyn, F.1
Speleman, F.2
De Paepe, A.3
Vandesompele, J.4
-
46
-
-
33644878359
-
RTPrimerDB: The real-time PCR primer and probe database, major update 2006
-
Pattyn, F., Robbrecht, P., De Paepe, A., Speleman, F. & Vandesompele, J. RTPrimerDB: The real-time PCR primer and probe database, major update 2006. Nucleic Acids Res. 34, 684-688 (2006).
-
(2006)
Nucleic Acids Res
, vol.34
, pp. 684-688
-
-
Pattyn, F.1
Robbrecht, P.2
De Paepe, A.3
Speleman, F.4
Vandesompele, J.5
-
47
-
-
85074914953
-
PCR primer bank for quantitative gene expression analysis
-
Wang, X. & Seed, B. A PCR primer bank for quantitative gene expression analysis. Nucleic Acids Res. 31, 154 (2003).
-
(2003)
Nucleic Acids Res
, vol.31
, pp. 154
-
-
Wang, X.1
Seed, B.A.2
-
48
-
-
33745160325
-
Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR
-
Reynisson, E., Josefsen, M.H., Krause, M. & Hoorfar, J. Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR: J. Microbiol. Methods 66, 206-216 (2005).
-
(2005)
J. Microbiol. Methods
, vol.66
, pp. 206-216
-
-
Reynisson, E.1
Josefsen, M.H.2
Krause, M.3
Hoorfar, J.4
-
49
-
-
0029883357
-
Optimization of Dnase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR
-
Huang, Z.; Fasco, M.J. & Kaminsky, L.S. Optimization of Dnase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR. Biotechniques 20, 1012-1020 (1996).
-
(1996)
Biotechniques
, vol.20
, pp. 1012-1020
-
-
Huang, Z.1
Fasco, M.J.2
Kaminsky, L.S.3
-
51
-
-
28644452204
-
Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces
-
Imbeaud, S. et al. Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids Res. 33, 56 (2005).
-
(2005)
Nucleic Acids Res
, vol.33
, pp. 56
-
-
Imbeaud, S.1
-
53
-
-
21344471138
-
Real-time RT-PCR normalization; strategies and considerations
-
Huggett, J., Dheda, K., Bustin, S. & Zumla, A. Real-time RT-PCR normalization; strategies and considerations. Genes Immun. 6 279-284 (2005).
-
(2005)
Genes Immun
, vol.6
, pp. 279-284
-
-
Huggett, J.1
Dheda, K.2
Bustin, S.3
Zumla, A.4
-
54
-
-
29144510503
-
Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos
-
Goossens, K. et al. Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos. BMC Dev. Biol. 5, 27 (2005).
-
(2005)
BMC Dev. Biol
, vol.5
, pp. 27
-
-
Goossens, K.1
-
55
-
-
0037109753
-
Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
-
Tricarico, C. et al. Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal.Biochem. 309, 293-300 (2002).
-
(2002)
Anal.Biochem
, vol.309
, pp. 293-300
-
-
Tricarico, C.1
-
56
-
-
27644563899
-
The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
-
Dheda, K. et al. The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal. Biochem. 344, 141-143 (2005).
-
(2005)
Anal. Biochem
, vol.344
, pp. 141-143
-
-
Dheda, K.1
-
57
-
-
24944584586
-
-
Impact of RNA quality on reference gene expression stability
-
Perez-Novo, C.A. et al. Impact of RNA quality on reference gene expression stability. Biotechniques 39 52, 54, 56 (2005).
-
(2005)
Biotechniques
, vol.39
-
-
Perez-Novo, C.A.1
-
58
-
-
0037129827
-
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
-
Vandesompele, J. et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3, 0034.1-0034.11 (2002).
-
(2002)
Genome Biol
, vol.3
-
-
Vandesompele, J.1
-
59
-
-
1842483281
-
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations
-
Pfaffl, M.W., Tichopad, A., Prgomet, C. & Neuvians, T. P. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 26, 509-515 (2004).
-
(2004)
Biotechnol. Lett
, vol.26
, pp. 509-515
-
-
Pfaffl, M.W.1
Tichopad, A.2
Prgomet, C.3
Neuvians, T.P.4
-
60
-
-
33646168728
-
Per cell normalization method for mRNA measurement by quantitative PCR and microarrays
-
Kanno, J. et al. "Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays. BMC Genomics 7 64 (2006).
-
(2006)
BMC Genomics
, vol.7
, pp. 64
-
-
Kanno, J.1
-
61
-
-
0030977377
-
Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion
-
Bauer, P., Rolfs, A., Regitz-Zagrosek, V., Hildebrandt A. & Fleck, E. Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion. Biotechniques 22, 1128-1132 (1997).
-
(1997)
Biotechniques
, vol.22
, pp. 1128-1132
-
-
Bauer, P.1
Rolfs, A.2
Regitz-Zagrosek, V.3
Hildebrandt, A.4
Fleck, E.5
-
62
-
-
2342510407
-
IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin
-
Nemeth, E. et al. IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin. J. Clin. Invest. 113, 1271-1276 (2004).
-
(2004)
J. Clin. Invest
, vol.113
, pp. 1271-1276
-
-
Nemeth, E.1
|