메뉴 건너뛰기




Volumn 1, Issue 3, 2006, Pages 1559-1582

Quantification of mRNA using real-time RT-PCR

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

EID: 33847643776     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.236     Document Type: Article
Times cited : (1934)

References (62)
  • 1
    • 0029964835 scopus 로고    scopus 로고
    • A novel method for real time quantitative RT-PCR
    • Gibson, U.E., Heid, C.A. & Williams, P.M. A novel method for real time quantitative RT-PCR. Genome Res. 6, 995-1001 (1996).
    • (1996) Genome Res , vol.6 , pp. 995-1001
    • Gibson, U.E.1    Heid, C.A.2    Williams, P.M.3
  • 2
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • Bustin, S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25, 169-193 (2000).
    • (2000) J. Mol. Endocrinol , vol.25 , pp. 169-193
    • Bustin, S.A.1
  • 3
    • 0036278859 scopus 로고    scopus 로고
    • Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream
    • Ginzinger, D.G. Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream. Exp. Hematol. 30, 503-512 (2002).
    • (2002) Exp. Hematol , vol.30 , pp. 503-512
    • Ginzinger, D.G.1
  • 4
    • 14044265394 scopus 로고    scopus 로고
    • Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
    • Bustin, S.A. & Nolan, T. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Tech. 15, 155-66 (2004).
    • (2004) J. Biomol. Tech , vol.15 , pp. 155-166
    • Bustin, S.A.1    Nolan, T.2
  • 5
    • 1542328972 scopus 로고    scopus 로고
    • Properties of the reverse transcription reaction in mRNA quantification
    • Stahlberg, A., Hakansson, J., Xian, X., Semb, H. & Kubista, M. Properties of the reverse transcription reaction in mRNA quantification. Clin. Chem. 50, 509-515 (2004).
    • (2004) Clin. Chem , vol.50 , pp. 509-515
    • Stahlberg, A.1    Hakansson, J.2    Xian, X.3    Semb, H.4    Kubista, M.5
  • 6
    • 4344564046 scopus 로고    scopus 로고
    • Comparison of reverse transcriptases in gene expression analysis
    • Stahlberg, A., Kubista, M. & Pfaffl, M. Comparison of reverse transcriptases in gene expression analysis. Clin. Chem. 50, 1678-1680 (2004).
    • (2004) Clin. Chem , vol.50 , pp. 1678-1680
    • Stahlberg, A.1    Kubista, M.2    Pfaffl, M.3
  • 7
    • 0036668567 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems
    • Bustin, S.A. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems. J. Mol. Endocrinol. 29, 23-39 (2002).
    • (2002) J. Mol. Endocrinol , vol.29 , pp. 23-39
    • Bustin, S.A.1
  • 8
    • 26844512816 scopus 로고    scopus 로고
    • Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis
    • Bustin, S.A. & Mueller, R. Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. Clin. Sci. (Lond.) 109, 365-379 (2005).
    • (2005) Clin. Sci. (Lond.) , vol.109 , pp. 365-379
    • Bustin, S.A.1    Mueller, R.2
  • 9
    • 33644917430 scopus 로고    scopus 로고
    • Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer
    • Bustin, S.A. & Mueller, R. Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer. Mol. Aspects Med. 27, 192-223 (2006).
    • (2006) Mol. Aspects Med , vol.27 , pp. 192-223
    • Bustin, S.A.1    Mueller, R.2
  • 10
    • 14744306436 scopus 로고
    • Simultaneous amplification and detection of specific DNA sequences
    • Higuchi, R., Dollinger, G., Walsh, P.S. & Griffith, R. Simultaneous amplification and detection of specific DNA sequences. Nat. Biotechnol. 10, 413-417 (1992).
    • (1992) Nat. Biotechnol , vol.10 , pp. 413-417
    • Higuchi, R.1    Dollinger, G.2    Walsh, P.S.3    Griffith, R.4
  • 11
    • 15744381713 scopus 로고
    • Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions
    • Higuchi, R., Fockler, C., Dollinger, G. & Watson, R. Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions. Biotechnol. (NY) 11, 1026-1030 (1993).
    • (1993) Biotechnol. (NY) , vol.11 , pp. 1026-1030
    • Higuchi, R.1    Fockler, C.2    Dollinger, G.3    Watson, R.4
  • 12
    • 22044458051 scopus 로고    scopus 로고
    • Real-time, fluorescence-based quantitative PCR: A snapshot of current procedures and preferences
    • Bustin, S.A. Real-time, fluorescence-based quantitative PCR: A snapshot of current procedures and preferences. Expert Rev. Mol. Diagn. 5, 493-498 (2005).
    • (2005) Expert Rev. Mol. Diagn , vol.5 , pp. 493-498
    • Bustin, S.A.1
  • 15
    • 33644975004 scopus 로고    scopus 로고
    • RNA integrity and the effect on the real-time qRT-PCR performance
    • Fleige, S. & Pfaffl, M.W. RNA integrity and the effect on the real-time qRT-PCR performance. Mol. Aspects Med. 27, 126-139 (2006).
    • (2006) Mol. Aspects Med , vol.27 , pp. 126-139
    • Fleige, S.1    Pfaffl, M.W.2
  • 16
    • 33645241407 scopus 로고    scopus 로고
    • The RIN: An RNA integrity number for assigning integrity values to RNA measurements
    • Schroeder, A. et al. The RIN: An RNA integrity number for assigning integrity values to RNA measurements. BMC Mol. Biol. 7, 3 (2006).
    • (2006) BMC Mol. Biol , vol.7 , pp. 3
    • Schroeder, A.1
  • 17
    • 0345257783 scopus 로고    scopus 로고
    • Chipping away at the chip bias: RNA degradation in microarray analysis
    • Auer, H. et al. Chipping away at the chip bias: RNA degradation in microarray analysis. Nat. Genet. 35, 292-293 (2003).
    • (2003) Nat. Genet , vol.35 , pp. 292-293
    • Auer, H.1
  • 19
    • 10744228596 scopus 로고    scopus 로고
    • Prevalence of selective inhibition of HPV-16 DNA amplification in cervicovaginal lavages
    • Lefevre, J., Hankins, C., Pourreaux, K., Voyer, H. & Coutlee, F. Prevalence of selective inhibition of HPV-16 DNA amplification in cervicovaginal lavages. J. Med. Virol. 72, 132-137 (2004).
    • (2004) J. Med. Virol , vol.72 , pp. 132-137
    • Lefevre, J.1    Hankins, C.2    Pourreaux, K.3    Voyer, H.4    Coutlee, F.5
  • 20
    • 1242338153 scopus 로고    scopus 로고
    • Comparison of two methods for the detection of hepatitis A virus in clam samples (Tapes spp.) by reverse transcription-nested PCR
    • Sunen, E., Casas, N., Moreno, B. & Zigorraga, C. Comparison of two methods for the detection of hepatitis A virus in clam samples (Tapes spp.) by reverse transcription-nested PCR. Int. J. Food Microbiol. 91, 147-154 (2004).
    • (2004) Int. J. Food Microbiol , vol.91 , pp. 147-154
    • Sunen, E.1    Casas, N.2    Moreno, B.3    Zigorraga, C.4
  • 21
    • 1642497870 scopus 로고    scopus 로고
    • Removal of PCR inhibitors using dielectrophoresis as a selective filter in a microsystem
    • Perch-Nielsen, I.R., Bang, D.D., Poulsen, C.R., El-Ali, J. & Wolff, A. Removal of PCR inhibitors using dielectrophoresis as a selective filter in a microsystem. Lab Chip 3, 212-216 (2003).
    • (2003) Lab Chip , vol.3 , pp. 212-216
    • Perch-Nielsen, I.R.1    Bang, D.D.2    Poulsen, C.R.3    El-Ali, J.4    Wolff, A.5
  • 22
    • 15444364560 scopus 로고    scopus 로고
    • Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors
    • Jiang, J., Alderisio, K.A., Singh, A. & Xiao, L. Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors. Appl. Environ. Microbiol. 71, 1135-1141 (2005).
    • (2005) Appl. Environ. Microbiol , vol.71 , pp. 1135-1141
    • Jiang, J.1    Alderisio, K.A.2    Singh, A.3    Xiao, L.4
  • 23
    • 0141482157 scopus 로고    scopus 로고
    • Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage
    • Guy, R.A., Payment, P., Krull, U.J. & Horgen, P.A. Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Appl. Environ. Microbiol. 69, 5178-5185 (2003).
    • (2003) Appl. Environ. Microbiol , vol.69 , pp. 5178-5185
    • Guy, R.A.1    Payment, P.2    Krull, U.J.3    Horgen, P.A.4
  • 24
    • 15444365256 scopus 로고    scopus 로고
    • Quantitative real-time PCR for cancer detection: The lymphoma case
    • Stahlberg, A., Zoric, N., Aman, P. &, Kubista, M. Quantitative real-time PCR for cancer detection: The lymphoma case. Expert Rev. Mol. Diagn. 5, 221-230 (2005).
    • (2005) Expert Rev. Mol. Diagn , vol.5 , pp. 221-230
    • Stahlberg, A.1    Zoric, N.2    Aman, P.3    Kubista, M.4
  • 25
    • 0037230778 scopus 로고    scopus 로고
    • Quantitative real-time PCR method for detection of B-lymphocyte monoclonality by comparison of kappa and lambda immunoglobulin light chain expression
    • Stahlberg, A., Aman, P., Ridell, B., Mostad, P. & Kubista, M. Quantitative real-time PCR method for detection of B-lymphocyte monoclonality by comparison of kappa and lambda immunoglobulin light chain expression. Clin. Chem. 49, 51-59 (2003).
    • (2003) Clin. Chem , vol.49 , pp. 51-59
    • Stahlberg, A.1    Aman, P.2    Ridell, B.3    Mostad, P.4    Kubista, M.5
  • 26
    • 1542356381 scopus 로고    scopus 로고
    • Standardized determination of real-time PCR efficiency from a single reaction set-up
    • Tichopad, A., Dilger, M., Schwarz, G. & Pfaffl, M.W. Standardized determination of real-time PCR efficiency from a single reaction set-up. Nucleic Acids Res. 31, e122 (2003).
    • (2003) Nucleic Acids Res , vol.31
    • Tichopad, A.1    Dilger, M.2    Schwarz, G.3    Pfaffl, M.W.4
  • 27
    • 0037435395 scopus 로고    scopus 로고
    • Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
    • Ramakers, C., Ruijter, J.M., Deprez, R.H. & Moorman, A.F. Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci. Lett. 339, 62-66 (2003).
    • (2003) Neurosci. Lett , vol.339 , pp. 62-66
    • Ramakers, C.1    Ruijter, J.M.2    Deprez, R.H.3    Moorman, A.F.4
  • 28
    • 0036289137 scopus 로고    scopus 로고
    • Validation of a quantitative method real time PCR kinetics
    • Liu, W. & Saint D.A. Validation of a quantitative method real time PCR kinetics. Biochem. Biophys. Res. Commun. 294, 347-353 (2002).
    • (2002) Biochem. Biophys. Res. Commun , vol.294 , pp. 347-353
    • Liu, W.1    Saint, D.A.2
  • 29
    • 0031784953 scopus 로고    scopus 로고
    • Armored RNA technology for production of ribonuclease-resistant viral RNA controls and standards
    • Pasloske, S.L., Walkerpeach, C.R., Obermoeller, R.D., Winkler, M. & DuBois, D.B. Armored RNA technology for production of ribonuclease-resistant viral RNA controls and standards. J. Clin. Microbiol. 36, 3590-3594 (1998).
    • (1998) J. Clin. Microbiol , vol.36 , pp. 3590-3594
    • Pasloske, S.L.1    Walkerpeach, C.R.2    Obermoeller, R.D.3    Winkler, M.4    DuBois, D.B.5
  • 30
    • 0038642772 scopus 로고    scopus 로고
    • Description of a multiplex Bordetella pertussis and Bordetella parapertussis LightCycler PCR assay with inhibition control
    • Cloud, J.L et al. Description of a multiplex Bordetella pertussis and Bordetella parapertussis LightCycler PCR assay with inhibition control. Diagn. Microbiol. Infect. Dis. 46, 189-195 (2003).
    • (2003) Diagn. Microbiol. Infect. Dis , vol.46 , pp. 189-195
    • Cloud, J.L.1
  • 31
    • 33645293441 scopus 로고    scopus 로고
    • SPUD: A qPCR assay for the detection of inhibitors in nucleic acid preparations
    • Nolan, T., Hands, R.E., Ogunkolade, B.W. & Bustin, S.A. SPUD: A qPCR assay for the detection of inhibitors in nucleic acid preparations. Anal. Biochem. 351, 308-310 (2006).
    • (2006) Anal. Biochem , vol.351 , pp. 308-310
    • Nolan, T.1    Hands, R.E.2    Ogunkolade, B.W.3    Bustin, S.A.4
  • 32
    • 0028170672 scopus 로고
    • Comparison of M-MLV reverse transcriptase and Tth polymerase activity in RT-PCR of samples with low virus burden
    • Cusi, M.G., Valassina, M. & Valensin, P.E. Comparison of M-MLV reverse transcriptase and Tth polymerase activity in RT-PCR of samples with low virus burden. Biotechniques 17, 1034-1036 (1994).
    • (1994) Biotechniques , vol.17 , pp. 1034-1036
    • Cusi, M.G.1    Valassina, M.2    Valensin, P.E.3
  • 33
    • 0029940698 scopus 로고    scopus 로고
    • Sensitivity of tyrosinase mRNA detection-by RT-PCR: RTth DNA polymerase vs. MMLV-RT and AmpliTaq polymerase
    • Juhasz, A., Ravi, S. & O'Connell, C.D. Sensitivity of tyrosinase mRNA detection-by RT-PCR: RTth DNA polymerase vs. MMLV-RT and AmpliTaq polymerase. Biotechniques 20, 592-600 (1996).
    • (1996) Biotechniques , vol.20 , pp. 592-600
    • Juhasz, A.1    Ravi, S.2    O'Connell, C.D.3
  • 34
    • 0028598720 scopus 로고
    • Evaluation of a 'one tube' reverse transcription-polymerase chain reaction for the detection of ruminant pestiviruses
    • Easton, L.A., Vilcek, S. & Nettleton, P.F. Evaluation of a 'one tube' reverse transcription-polymerase chain reaction for the detection of ruminant pestiviruses. J. Virol. Methods 50, 343-348 (1994).
    • (1994) J. Virol. Methods , vol.50 , pp. 343-348
    • Easton, L.A.1    Vilcek, S.2    Nettleton, P.F.3
  • 35
    • 0036534582 scopus 로고    scopus 로고
    • Vandesompele, J., De Paepe, A. & Speleman, F. Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR green I-real-time RT-PCR 303, 95-98 (2002).
    • Vandesompele, J., De Paepe, A. & Speleman, F. Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR green I-real-time RT-PCR 303, 95-98 (2002).
  • 37
    • 29144495882 scopus 로고    scopus 로고
    • PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
    • Suslov, O. & Steindler, D.A. PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency. Nucleic Acids Res 33, e181 (2005).
    • (2005) Nucleic Acids Res , vol.33
    • Suslov, O.1    Steindler, D.A.2
  • 38
    • 12344305007 scopus 로고    scopus 로고
    • Gestational profile of Na+/H+ exchanger and Cl/HCO3- anion exchanger mRNA expression in placenta using real-time QPCR
    • Lacey, H.A., Nolan, T., Greenwood, S.L., Glazier, J.D. & Sibley, C.P. Gestational profile of Na+/H+ exchanger and Cl/HCO3- anion exchanger mRNA expression in placenta using real-time QPCR. Placenta 26 93-98 (2005).
    • (2005) Placenta , vol.26 , pp. 93-98
    • Lacey, H.A.1    Nolan, T.2    Greenwood, S.L.3    Glazier, J.D.4    Sibley, C.P.5
  • 39
    • 33745482592 scopus 로고    scopus 로고
    • Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA
    • Stangegaard, M., Dufva, I.H. & Dufva, M. Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA. Biotechniques 40, 649-657 (2006).
    • (2006) Biotechniques , vol.40 , pp. 649-657
    • Stangegaard, M.1    Dufva, I.H.2    Dufva, M.3
  • 40
    • 34648863662 scopus 로고    scopus 로고
    • Lewis, F. & Maughan, N.J. Extraction of Total RNA from Formalin-Fixed Paraffin-Embedded Tissue. in A-Z of quantitative PCR (ed. Bustin, S.A.) (IUL, La Jolla, California, 2004).
    • Lewis, F. & Maughan, N.J. Extraction of Total RNA from Formalin-Fixed Paraffin-Embedded Tissue. in A-Z of quantitative PCR (ed. Bustin, S.A.) (IUL, La Jolla, California, 2004).
  • 41
    • 0036667911 scopus 로고    scopus 로고
    • Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions
    • Lekanne Deprez, R.H., Fijnvandraat, A.C., Ruijter, J.M. & Moorman, A.F. Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions. Anal. Biochem. 307, 63-69 (2002).
    • (2002) Anal. Biochem , vol.307 , pp. 63-69
    • Lekanne Deprez, R.H.1    Fijnvandraat, A.C.2    Ruijter, J.M.3    Moorman, A.F.4
  • 42
    • 29144484144 scopus 로고    scopus 로고
    • Multiplexed tandem PCR: Gene profiling from small amounts of RNA using SYBR Green detection
    • Stanley, K.K. & Szewczuk, E. Multiplexed tandem PCR: Gene profiling from small amounts of RNA using SYBR Green detection. Nucleic Acids Res 33, e180 (2005).
    • (2005) Nucleic Acids Res , vol.33
    • Stanley, K.K.1    Szewczuk, E.2
  • 43
    • 29244475713 scopus 로고    scopus 로고
    • Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability
    • Hilscher, C., Vahrson, W. & Dittmer, D.P. Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability. Nucleic Acids Res. 33, e182 (2005).
    • (2005) Nucleic Acids Res , vol.33
    • Hilscher, C.1    Vahrson, W.2    Dittmer, D.P.3
  • 44
    • 13244265562 scopus 로고    scopus 로고
    • Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell
    • Hartshorn, C., Anshelevich, A. & Wangh, L.J. Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell. BMC Biotechnol 5, 2 (2005).
    • (2005) BMC Biotechnol , vol.5 , pp. 2
    • Hartshorn, C.1    Anshelevich, A.2    Wangh, L.J.3
  • 47
    • 85074914953 scopus 로고    scopus 로고
    • PCR primer bank for quantitative gene expression analysis
    • Wang, X. & Seed, B. A PCR primer bank for quantitative gene expression analysis. Nucleic Acids Res. 31, 154 (2003).
    • (2003) Nucleic Acids Res , vol.31 , pp. 154
    • Wang, X.1    Seed, B.A.2
  • 48
    • 33745160325 scopus 로고    scopus 로고
    • Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR
    • Reynisson, E., Josefsen, M.H., Krause, M. & Hoorfar, J. Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR: J. Microbiol. Methods 66, 206-216 (2005).
    • (2005) J. Microbiol. Methods , vol.66 , pp. 206-216
    • Reynisson, E.1    Josefsen, M.H.2    Krause, M.3    Hoorfar, J.4
  • 49
    • 0029883357 scopus 로고    scopus 로고
    • Optimization of Dnase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR
    • Huang, Z.; Fasco, M.J. & Kaminsky, L.S. Optimization of Dnase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR. Biotechniques 20, 1012-1020 (1996).
    • (1996) Biotechniques , vol.20 , pp. 1012-1020
    • Huang, Z.1    Fasco, M.J.2    Kaminsky, L.S.3
  • 51
    • 28644452204 scopus 로고    scopus 로고
    • Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces
    • Imbeaud, S. et al. Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids Res. 33, 56 (2005).
    • (2005) Nucleic Acids Res , vol.33 , pp. 56
    • Imbeaud, S.1
  • 53
    • 21344471138 scopus 로고    scopus 로고
    • Real-time RT-PCR normalization; strategies and considerations
    • Huggett, J., Dheda, K., Bustin, S. & Zumla, A. Real-time RT-PCR normalization; strategies and considerations. Genes Immun. 6 279-284 (2005).
    • (2005) Genes Immun , vol.6 , pp. 279-284
    • Huggett, J.1    Dheda, K.2    Bustin, S.3    Zumla, A.4
  • 54
    • 29144510503 scopus 로고    scopus 로고
    • Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos
    • Goossens, K. et al. Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos. BMC Dev. Biol. 5, 27 (2005).
    • (2005) BMC Dev. Biol , vol.5 , pp. 27
    • Goossens, K.1
  • 55
    • 0037109753 scopus 로고    scopus 로고
    • Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
    • Tricarico, C. et al. Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal.Biochem. 309, 293-300 (2002).
    • (2002) Anal.Biochem , vol.309 , pp. 293-300
    • Tricarico, C.1
  • 56
    • 27644563899 scopus 로고    scopus 로고
    • The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
    • Dheda, K. et al. The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal. Biochem. 344, 141-143 (2005).
    • (2005) Anal. Biochem , vol.344 , pp. 141-143
    • Dheda, K.1
  • 57
    • 24944584586 scopus 로고    scopus 로고
    • Impact of RNA quality on reference gene expression stability
    • Perez-Novo, C.A. et al. Impact of RNA quality on reference gene expression stability. Biotechniques 39 52, 54, 56 (2005).
    • (2005) Biotechniques , vol.39
    • Perez-Novo, C.A.1
  • 58
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • Vandesompele, J. et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3, 0034.1-0034.11 (2002).
    • (2002) Genome Biol , vol.3
    • Vandesompele, J.1
  • 59
    • 1842483281 scopus 로고    scopus 로고
    • Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations
    • Pfaffl, M.W., Tichopad, A., Prgomet, C. & Neuvians, T. P. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 26, 509-515 (2004).
    • (2004) Biotechnol. Lett , vol.26 , pp. 509-515
    • Pfaffl, M.W.1    Tichopad, A.2    Prgomet, C.3    Neuvians, T.P.4
  • 60
    • 33646168728 scopus 로고    scopus 로고
    • Per cell normalization method for mRNA measurement by quantitative PCR and microarrays
    • Kanno, J. et al. "Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays. BMC Genomics 7 64 (2006).
    • (2006) BMC Genomics , vol.7 , pp. 64
    • Kanno, J.1
  • 61
    • 0030977377 scopus 로고    scopus 로고
    • Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion
    • Bauer, P., Rolfs, A., Regitz-Zagrosek, V., Hildebrandt A. & Fleck, E. Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion. Biotechniques 22, 1128-1132 (1997).
    • (1997) Biotechniques , vol.22 , pp. 1128-1132
    • Bauer, P.1    Rolfs, A.2    Regitz-Zagrosek, V.3    Hildebrandt, A.4    Fleck, E.5
  • 62
    • 2342510407 scopus 로고    scopus 로고
    • IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin
    • Nemeth, E. et al. IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin. J. Clin. Invest. 113, 1271-1276 (2004).
    • (2004) J. Clin. Invest , vol.113 , pp. 1271-1276
    • Nemeth, E.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.