Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies

Analytical Biochemistry

Volumn 309, Issue 2, 2002, Pages 293-300

  Tricarico, Carmela ^a   Pinzani, Pamela ^a   Bianchi, Simonetta ^a   Paglierani, Milena ^a   Distante, Vito ^a   Pazzagli, Mario ^a   Bustin, Stephen A ^b   Orlando, Claudio ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

^b UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOPSY; RNA; TISSUE; TRANSCRIPTION;

DIFFERENT TREATMENTS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; MICROVESSEL DENSITY; QUANTITATIVE RESULT; QUANTITATIVE REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; RIBOSOMAL RNA (RRNA); VASCULAR ENDOTHELIAL GROWTH FACTOR;

POLYMERASE CHAIN REACTION;

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MESSENGER RNA; RIBOSOME RNA; VASCULOTROPIN;

ACCURACY; ARTICLE; BIOPSY; COLON BIOPSY; CORRELATION ANALYSIS; GENE CONTROL; GENE EXPRESSION; HOUSEKEEPING GENE; HUMAN; HUMAN TISSUE; MICROVASCULATURE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0037109753     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00311-1     Document Type: Article

References (26)

1. C. Orlando, P. Pinzani, M. Pazzagli, Developments in quantitative PCR, Clin. Chem. Lab. Med. 36 (1998) 255-269.
2. S.A. Bustin, Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays, J. Mol. Endocrinol. 25 (2000) 169-193.
3. S.A. Bustin, Quantification of mRNA using real-time RT-PCR: Trends and problems, J. Mol. Endocrinol. 28 (2002) 23-39.
4. T. Suzuki, P.J. Higgins, D.R. Crawford, Control selection for RNA quantitation, Biotechniques 29 (2000) 332-337.
5. T.D. Schmittgen, B.A. Zakrajsek, Effect of experimental treatment on housekeeping gene expression: Validation by real-time, quantitative RT-PCR, J. Biochem. Biophys. Methods 46 (2000) 69-81.
6. J. Lupberger, K.A. Kreuzer, G. Baskaynak, U.R. Peters, P. le Coutre, C.A. Schmidt, Quantitative analysis of beta-actin, beta-2-microglobulin and porphobilinogen deaminase mRNA and their comparison as control transcripts for RT-PCR, Mol. Cell. Probes 16 (2002) 25-30.
7. H.K. Hamalainen, J.C. Tubman, S. Vikman, T. Kyrola, E. Ylikoski, J.A. Warrington, R. Lahesmaa, Identification and validation of endogenous reference genes for expression profiling of T helper cell differentiation by quantitative real-time RT-PCR, Anal. Biochem. 299 (2001) 63-70.
8. S.A. Bustin, V.G. Gyselman, N.S. Williams, S. Dorudi, Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients, Br. J. Cancer 79 (1999) 1813-1820.
9. C. Tricarico, B. Salvadori, D. Villari, G. Nicita, M.A. Della, P. Pinzani, M. Ziche, M. Pazzagli, Quantitative RT-PCR assay for VEGF mRNA in human tumors of the kidney, Int. J. Biol. Markers 14 (1999) 247-250.
10. Y. Seo, H. Baba, T. Fukuda, M. Takashima, K. Sugimachi, High expression of vascular endothelial growth factor is associated with liver metastasis and a poor prognosis for patients with ductal pancreatic adenocarcinoma, Cancer 88 (2000) 2239-2245.
11. G. Morphopoulos, M. Pearson, W.D. Ryder, A. Howell, M. Harris, Tumour angiogenesis as a prognostic marker in infiltrating lobular carcinoma of the breast, J. Pathol. 180 (1996) 44-49.
12. P.A. Scott, K. Smith, R. Poulsom, A. De Benedetti, R. Bicknell, A.L. Harris, Differential expression of vascular endothelial growth factor mRNA vs protein isoform expression in human breast cancer and relationship to eiF-4E, Br. J. Cancer 77 (1998) 2120-2128.
13. F. Cianchi, C. Cortesini, P. Bechi, O. Fantappie, L. Messerini, A. Vannacci, I. Sardi, G. Baroni, V. Boddi, R. Mazzanti, E. Masini, Up-regulation of cyclooxygenase2 gene expression correlates with tumor angiogenesis in human colorectal cancer, Gastroenterology 121 (2001) 1339-1347.
14. 2÷-activated chloride channel genes CLCA1 and CLCA2 is downregulated in human colorectal cancer, DNA Cell Biol. 20 (2001) 331-338.
15. S.R. Li, S. Dorudi, S.A. Bustin, Elevated levels of RanBP7 mRNA in colorectal carcinoma are associated with increased proliferation and are similar to the transcription pattern of the proto-oncogene c-myc, Biochem. Biophys. Res. Commun. 271 (2000) 322-329.
16. S.R. Li, V.G. Gyselman, O. Lalude, S. Dorudi, S.A. Bustin, Transcription of the inositol polyphosphate 1-phosphatase gene (INPP1) is upregulated in human colorectal cancer, Mol. Carcinog. 27 (2000) 322-329.
17. M. Korbonits, S.A. Bustin, M. Kojima, S. Jordan, E.F. Adams, D.G. Lowe, K. Kangawa, A.B. Grossman, The expression of the growth hormone secretagogue receptor ligand ghrelin in normal and abnormal human pituitary and other neuroendocrine tumors, J. Clin. Endocrinol. Metab. 86 (2001) 881-887.
18. J. Vandesompele, K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, A. De Paepe, F. Speleman, Accurate normalization of real-time geometric averaging of multiple internal control genes, Genome Biol. 3 (2002) research0034.1-research0034.11.
19. D.R. Smith, T. Myint, H.S. Goh, Over-expression of the c-myc proto-oncogene in colorectal carcinoma, Br. J. Cancer 68 (1993) 407-413.
20. K. Sato, M. Miyahara, T. Saito, M. Kobayashi, c-myc mRNA overexpression is associated with lymph node metastasis in colorectal cancer, Eur. J. Cancer 30A (1994) 1113-1117.
21. C.F. Rochlitz, R. Herrmann, E. de Kant, Overexpression and amplification of c-myc during progression of human colorectal cancer, Oncology 53 (1996) 448-454.
22. T.R. Regnault, R.J. Orbus, B. de Vrijer, M.L. Davidsen, H.L. Galan, R.B. Wilkening, R.V. Anthony, Placental expression of VEGF, PIGF and their receptors in a model of placental insufficiency-intrauterine growth restriction (PI-IUGR), Placenta 23 (2002) 132-144.
23. K.K. Graven R.F. Troxler, H. Kornfeld, M.V. Panchenko, H.W. Farber, Regulation of endothelial cell glyceraldehyde-3-phosphate dehydrogenase expression by hypoxia, J. Biol. Chem. 269 (1994) 24446-24453.
24. H. Zhong, J.W. Simons, Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia, Biochem. Biophys. Res. Commun. 259 (1999) 523-526.
25. L.S. Ziemer, C.J. Koch, A. Maity, D.P. Magarelli, A.M. Horan, S.M. Evans, Hypoxia and VEGF mRNA expression in human tumors, Neoplasia 3 (2001) 500-508.
26. S. Lu, X. Gu, S. Hoestje, D.E. Epner, Identification of an additional hypoxia responsive element in the glyceraldehyde-3-phosphate dehydrogenase gene promoter, Biochim. Biophys. Acta 1574 (2002) 152-156.