Optimization of DNase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR

BioTechniques

Volumn 20, Issue 6, 1996, Pages 1012-1020

  Huang, Zeqi ^a   Fasco, Michael J ^a,b   Kaminsky, Laurence S ^a,b,c  

^a STATE UNIVERSITY OF NEW YORK   (United States)

^b NEW YORK STATE DEPARTMENT OF HEALTH   (United States)

^c WADSWORTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; COMPETITIVE INHIBITION; DENATURATION; DNA RNA HYBRIDIZATION; ENZYME INACTIVATION; HUMAN; HUMAN CELL; QUANTITATIVE DIAGNOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSCRIPTION;

BASE SEQUENCE; DEOXYRIBONUCLEASE I; DNA; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA, MESSENGER; TUMOR CELLS, CULTURED;

EID: 0029883357     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96206st02     Document Type: Article

References (6)

1. Belinsky, M. and A.K. Jaiswal. 1993. NAD(P)H:Quinone oxidoreductase 1 (DT-diaphorase) expression in normal and tumor tissues. Cancer Metastasis Rev. 12:103-117.
2. Dilworth, D.D. and J.R. McCarrey. 1992. Single-step elimination of contaminating DNA prior to reverse transcriptase PCR. PCR Methods Appl. 1:279-282.
3. Fasco, M.J., C.P. Treanor, S. Spivack, H.L. Figge and L.S. Kaminsky. 1995. Quantitative RNA-polymerase chain reaction-DNA analysis by capillary electrophoresis and laser-induced fluorescence. Anal. Biochem. 224:140-147.
4. Liang, P., L. Averbouth and A.B. Pardee. 1993. Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization. Nucleic Acid Res. 21:3269-3275.
5. Rashtchian, A. 1994. Amplification of RNA. PCR Methods Appl. 4:S83-S91.
6. Rolfs, A., I. Schuller, U. Finckh and I. Weber-Rolfs. 1992. Reverse transcription/PCR (RT-PCR), p. 99-111. In A. Rolfs, I. Schuller, U. Finckh and I. Weber-Rolfs (Eds.), PCR: Clinical Diagnostics and Research. Springer-Verlag, Berlin.