Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR green I real-time RT-PCR

Analytical Biochemistry

Volumn 303, Issue 1, 2002, Pages 95-98

  Vandesompele, J ^a   De Paepe, Anne ^a   Speleman, Frank ^a  

^a GHENT UNIVERSITY HOSPITAL   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

REAL TIME RT-PCR; SYBR GREEN I;

GENES;

COMPLEMENTARY DNA; DIMER; DYE; PRIMER DNA; SYBR GREEN 1; TAQ POLYMERASE; UNCLASSIFIED DRUG;

ACCURACY; ARTIFACT; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA BINDING; DNA SYNTHESIS; GENE AMPLIFICATION; GENE EXPRESSION; HUMAN; MELTING POINT; NOTE; PRIORITY JOURNAL; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0036534582     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5564     Document Type: Article

References (8)

1. Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions
2. Real time quantitative PCR
3. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
4. Advances in quantitative PCR technology: 5′ nuclease assays
5. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
6. The elimination of primer-dimer accumulation in PCR
7. The DNA sequence of human chromosome 22
8. The DNA sequence of human chromosome 21: The chromosome 21 mapping and sequencing consortium