SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations

Analytical Biochemistry

Volumn 351, Issue 2, 2006, Pages 308-310

  Nolan, Tania ^a   Hands, Rebecca E ^b   Ogunkolade, William ^b   Bustin, Stephen A ^b,c  

^a Sigma Aldrich   (United Kingdom)

^b QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^c ROYAL LONDON HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

QUANTITATIVE PCR;

POLYMERASE CHAIN REACTION;

BILE SALT; COMPLEMENTARY DNA; FORMALDEHYDE; HEPARIN; IMMUNOGLOBULIN G; MESSENGER RNA; NUCLEIC ACID; NUCLEOTIDE; PARAFFIN; UREA; WATER;

ACCURACY; ARTICLE; FLUORESCENCE; GENE AMPLIFICATION; GENE EXPRESSION; GENE SEQUENCE; HEALTH SURVEY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; REPORTER GENE; REPRODUCIBILITY; RNA EXTRACTION; SENSITIVITY ANALYSIS;

EID: 33645293441     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.01.051     Document Type: Article

References (18)

1. S.A. Bustin, and T. Nolan Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction J. Biomol. Tech. 15 2004 155 166
2. T. Bar, A. Stahlberg, A. Muszta, and M. Kubista Kinetic outlier detection (KOD) in real-time PCR Nucleic Acids Res. 31 2003 e105
3. P. Radstrom, R. Knutsson, P. Wolffs, M. Lovenklev, and C. Lofstrom Pre-PCR processing: strategies to generate PCR-compatible samples Mol. Biotechnol. 26 2004 133 146
4. J. Lefevre, C. Hankins, K. Pourreaux, H. Voyer, and F. Coutlee Prevalence of selective inhibition of HPV-16 DNA amplification in cervicovaginal lavages J. Med. Virol. 72 2004 132 137
5. E. Sunen, N. Casas, B. Moreno, and C. Zigorraga Comparison of two methods for the detection of hepatitis A virus in clam samples (Tapes spp.) by reverse transcription-nested PCR Intl. J. Food Microbiol. 91 2004 147 154
6. I.R. Perch-Nielsen, D.D. Bang, C.R. Poulsen, J. El-Ali, and A. Wolff Removal of PCR inhibitors using dielectrophoresis as a selective filter in a microsystem Lab. Chip 3 2003 212 216
7. J. Jiang, K.A. Alderisio, A. Singh, and L. Xiao Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors Appl. Environ. Microbiol. 71 2005 1135 1141
8. R.A. Guy, P. Payment, U.J. Krull, and P.A. Horgen Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage Appl. Environ. Microbiol. 69 2003 5178 5185
9. A. Stahlberg, N. Zoric, P. Aman, and M. Kubista Quantitative real-time PCR for cancer detection: the lymphoma case Expert Rev. Mol. Diagn. 5 2005 221 230
10. S.A. Bustin Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences Expert Rev. Mol. Diagn. 5 2005 493 498
11. A. Stahlberg, P. Aman, B. Ridell, P. Mostad, and M. Kubista Quantitative real-time PCR method for detection of B-lymphocyte monoclonality by comparison of kappa and lambda immunoglobulin light chain expression Clin. Chem. 49 2003 51 59
12. A. Tichopad, M. Dilger, G. Schwarz, and M.W. Pfaffl Standardized determination of real-time PCR efficiency from a single reaction set-up Nucleic Acids Res. 31 2003 e122
13. C. Ramakers, J.M. Ruijter, R.H. Deprez, and A.F. Moorman Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data Neurosci. Lett. 339 2003 62 66
14. W. Liu, and D.A. Saint Validation of a quantitative method for real time PCR kinetics Biochem. Biophys. Res. Commun. 294 2002 347 353
15. B.L. Pasloske, C.R. Walkerpeach, R.D. Obermoeller, M. Winkler, and D.B. DuBois Armored RNA technology for production of ribonuclease-resistant viral RNA controls and standards J. Clin. Microbiol. 36 1998 3590 3594
16. S. Burggraf, and B. Olgemoller Simple technique for internal control of real-time amplification assays Clin. Chem. 50 2004 819 825
17. J.L. Cloud, W.C. Hymas, A. Turlak, A. Croft, U. Reischl, J.A. Daly, and K.C. Carroll Description of a multiplex Bordetella pertussis and Bordetella parapertussis LightCycler PCR assay with inhibition control Diagn. Microbiol. Infect. Dis. 46 2003 189 195
18. S.F. Altschul, W. Gish, W. Miller, E.W. Myers, and D.J. Lipman Basic local alignment search tool J. Mol. Biol. 215 1990 403 410