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Volumn 307, Issue 1, 2002, Pages 63-69
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Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions
a
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Author keywords
cDNA synthesis; DTT; mRNA expression; Quantification; Real time RT PCR; SYBR green I
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Indexed keywords
TRANSCRIPTION;
CDNA SYNTHESIS;
MRNA EXPRESSION;
QUANTIFICATION;
REAL TIME RT-PCR;
SYBR GREEN I;
POLYMERASE CHAIN REACTION;
COMPLEMENTARY DNA;
DITHIOTHREITOL;
DYE;
MESSENGER RNA;
SYBR GREEN 1;
UNCLASSIFIED DRUG;
DIAGNOSTIC AGENT;
DNA;
FLUORESCENT DYE;
MAGNESIUM CHLORIDE;
ORGANIC COMPOUND;
PRIMER DNA;
SYBR GREEN I;
ACCURACY;
ANIMAL CELL;
ARTICLE;
CONTROLLED STUDY;
DNA SYNTHESIS;
FLUORESCENCE;
GENE AMPLIFICATION;
GENE EXPRESSION;
MEDICAL RESEARCH;
MELTING POINT;
NONHUMAN;
NUCLEOTIDE SEQUENCE;
POLYMERASE CHAIN REACTION;
PRIORITY JOURNAL;
QUANTITATIVE ANALYSIS;
REACTION ANALYSIS;
REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;
SENSITIVITY AND SPECIFICITY;
ANIMAL;
BIOSYNTHESIS;
METHODOLOGY;
MOUSE;
REPRODUCIBILITY;
TEMPERATURE;
ANIMALS;
DNA;
DNA PRIMERS;
DNA, COMPLEMENTARY;
FLUORESCENT DYES;
MAGNESIUM CHLORIDE;
MICE;
ORGANIC CHEMICALS;
REPRODUCIBILITY OF RESULTS;
REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;
RNA, MESSENGER;
SENSITIVITY AND SPECIFICITY;
TEMPERATURE;
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EID: 0036667911
PISSN: 00032697
EISSN: None
Source Type: Journal
DOI: 10.1016/S0003-2697(02)00021-0 Document Type: Article |
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Times cited : (271)
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References (11)
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