Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Genome Biology

Volumn 3, Issue 7, 2002, Pages

  Vandesompele, Jo ^a   De Preter, Katleen ^a   Pattyn, Filip ^a   Poppe, Bruce ^a   Van Roy, Nadine ^a   De Paepe, Anne ^a   Speleman, Frank ^a  

^a GHENT UNIVERSITY HOSPITAL   (Belgium)

Author keywords

Additional Data File; Control Gene; Internal Control Gene; Nonspecific Variation; Pairwise Variation

Indexed keywords

ARTICLE; CONTROLLED STUDY; FEMALE; GENE CONTROL; GENE EXPRESSION; GENE EXPRESSION PROFILING; HOUSEKEEPING GENE; HUMAN; HUMAN TISSUE; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REPRODUCIBILITY; ALGORITHM; CELL CULTURE; GENETICS; METABOLISM; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARD; TIME;

COMPLEMENTARY DNA; RNA;

ALGORITHMS; DNA, COMPLEMENTARY; FEMALE; GENE EXPRESSION PROFILING; HUMANS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; TIME FACTORS; TUMOR CELLS, CULTURED;

EID: 0037129827     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/gb-2002-3-7-research0034     Document Type: Article

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