Sensitivity of tyrosinase mRNA detection by RT-PCR: rTth DNA polymerase vs. MMLV-RT and AmpliTaq® polymerase

BioTechniques

Volumn 20, Issue 4, 1996, Pages 592-600

  Juhasz, Agnes ^a   Ravi, Sasikala ^a   O'Connell, Catherine D ^a  

^a NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; MONOPHENOL MONOOXYGENASE; RNA DIRECTED DNA POLYMERASE;

ARTICLE; CANCER DIAGNOSIS; DIAGNOSTIC ACCURACY; ENZYME LOCALIZATION; GENE AMPLIFICATION; HUMAN; MELANOMA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SEQUENCE ANALYSIS;

ACTINS; BASE SEQUENCE; DNA-DIRECTED DNA POLYMERASE; MOLECULAR SEQUENCE DATA; MOLONEY MURINE LEUKEMIA VIRUS; MONOPHENOL MONOOXYGENASE; POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; THERMUS THERMOPHILUS;

EID: 0029940698     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. Bej, A.K. and M.H. Mahbubani. 1994. Thermostable DNA Polymerases for in vitro DNA amplifications, p.219-239. In H.G. Griffin and A. M. Griffin (Ed.), PCR Technology: Current Innovations. CRC Press, Boca Raton.
2. Cusi, M., G.M. Valassina and P.E. Valensin. 1994. Comparison of M-MLV reverse transcriptase and Tth polymerase activity in RT-PCR of samples with low virus burden. BioTechniques 17:1035-1036.
3. Hoon, D.S.B., Y. Wang, P.S. Dale, A.J. Conrad, P. Schmid, D. Garrison, C. Kuo, L.J. Foshag, A.J. Nizze and D.L. Morton. 1995. Detection of occult melanoma cells in blood with a multiple-marker polymerase chain reaction assay. J. Clin. Oncol. 13:2109-2116.
4. Mallet, F., G. Oriol, C. Mary, B. Verrier and B. Mandrand. 1995. Continuous RT-PCR using AMV-RT and Taq DNA polymerase: characterization and comparison to uncoupled procedures. BioTechniques 18:678-687.
5. Myers, T.W and D.H. Gelfand. 1991. Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 30:7661-7666.
6. Powell, L.M., S.C. Wallis, R.J. Pease, Y.H. Edwards, T.J. Knott and J. Scott. 1987. A novel form of tissue-specific RNA processing produces apolipoprotein-B48 in intestine. Cell 50:831-840.
7. Saiki, R.K., D.H. Gelfand, S. Stoffel, S.J. Scharf, R. Higuchi, G.T. Horn, K.B. Mullis and H.A. Erlich. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487-491.
8. Smith, B., P. Selby, J. Southgate, K. Pittman, C. Bradley and G.E. Blair. 1991. Detection of melanoma cells in peripheral blood by means of reverse transcriptase and polymerase chain reaction. Lancet 338:1227-1229.