-
1
-
-
0026510509
-
Reverse transcriptase inhibits Taq polymerase activity
-
Sellner,L.N., Coelen,R.J. and Mackenzie,J.S. (1992) Reverse transcriptase inhibits Taq polymerase activity. Nucleic Acids Res., 20, 1487-1490.
-
(1992)
Nucleic Acids Res.
, vol.20
, pp. 1487-1490
-
-
Sellner, L.N.1
Coelen, R.J.2
Mackenzie, J.S.3
-
2
-
-
0027478299
-
Reverse transcriptase can block polymerase chain reaction
-
Fehlmann,C., Krapf,R. and Solioz,M. (1993) Reverse transcriptase can block polymerase chain reaction. Clin. Chem., 39, 368-369.
-
(1993)
Clin. Chem.
, vol.39
, pp. 368-369
-
-
Fehlmann, C.1
Krapf, R.2
Solioz, M.3
-
3
-
-
0028568553
-
Reverse transcriptase can inhibit PCR and stimulate primer-dimer formation
-
Chumakov,K.M. (1994) Reverse transcriptase can inhibit PCR and stimulate primer-dimer formation. PCR Methods Appl., 4, 62-64.
-
(1994)
PCR Methods Appl.
, vol.4
, pp. 62-64
-
-
Chumakov, K.M.1
-
4
-
-
0031889693
-
Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR
-
Chandler,D.P., Wagnon,C.A. and Bolton,H.,Jr (1998) Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR. Appl. Environ. Microbiol., 64, 669-677.
-
(1998)
Appl. Environ. Microbiol.
, vol.64
, pp. 669-677
-
-
Chandler, D.P.1
Wagnon, C.A.2
Bolton Jr., H.3
-
5
-
-
0036724945
-
Improved quantitative real-time RT-PCR for expression profiling of individual cells
-
Liss,B. (2002) Improved quantitative real-time RT-PCR for expression profiling of individual cells. Nucleic Acids Res., 30, e89.
-
(2002)
Nucleic Acids Res.
, vol.30
-
-
Liss, B.1
-
6
-
-
0028042214
-
A coupled one-step reverse transcription PCR procedure for generation of full-length open reading frames
-
Aatsinki,J.T., Lakkakorpi,J.T., Pietila,E.M. and Rajaniemi,H.J. (1994) A coupled one-step reverse transcription PCR procedure for generation of full-length open reading frames. Biotechniques, 16, 282-284, 286-288.
-
(1994)
Biotechniques
, vol.16
, pp. 282-284
-
-
Aatsinki, J.T.1
Lakkakorpi, J.T.2
Pietila, E.M.3
Rajaniemi, H.J.4
-
7
-
-
0036216132
-
Evaluation of a homemade SYBR green I reaction mixture for real-time PCR quantification of gene expression
-
Karsai,A., Muller,S., Platz,S. and Hauser,M.T. (2002) Evaluation of a homemade SYBR green I reaction mixture for real-time PCR quantification of gene expression. Biotechniques, 232, 790-792, 794-796.
-
(2002)
Biotechniques
, vol.232
, pp. 790-792
-
-
Karsai, A.1
Muller, S.2
Platz, S.3
Hauser, M.T.4
-
8
-
-
0036667911
-
Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions
-
Deprez,R.H.L., Fijnvandraat,A.C., Ruijter,J.M. and Moorman,A.F.M. (2002) Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions. Anal. Biochem., 307, 63-69.
-
(2002)
Anal. Biochem.
, vol.307
, pp. 63-69
-
-
Deprez, R.H.L.1
Fijnvandraat, A.C.2
Ruijter, J.M.3
Moorman, A.F.M.4
-
9
-
-
0042121256
-
Mfold web server for nucleic acid folding and hybridization prediction
-
Zuker,M. (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res., 31, 3406-3415.
-
(2003)
Nucleic Acids Res.
, vol.31
, pp. 3406-3415
-
-
Zuker, M.1
-
10
-
-
3342925716
-
Thermal stability and cDNA synthesis capability of SuperScript III reverse transcriptase
-
25.1
-
Potter,J., Zheng,W. and Lee,J. (2003) Thermal stability and cDNA synthesis capability of SuperScript III reverse transcriptase. Focus, 25.1, 19-24.
-
(2003)
Focus
, pp. 19-24
-
-
Potter, J.1
Zheng, W.2
Lee, J.3
-
11
-
-
1542266463
-
Kinetic Outlier Detection (KOD) in real-time PCR
-
Bar,T., Stahlberg,A., Muszta,A. and Kubista,M. (2003) Kinetic Outlier Detection (KOD) in real-time PCR. Nucleic Acids Res., 31, e105.
-
(2003)
Nucleic Acids Res.
, vol.31
-
-
Bar, T.1
Stahlberg, A.2
Muszta, A.3
Kubista, M.4
-
12
-
-
14044265394
-
Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
-
Bustin,S.A. and Nolan,T. (2004) Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Tech., 15, 155-166.
-
(2004)
J. Biomol. Tech.
, vol.15
, pp. 155-166
-
-
Bustin, S.A.1
Nolan, T.2
-
13
-
-
0037129827
-
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
-
RESEARCH 0034. Epub
-
Vandesompele,J., De Preter,K., Pattyn,F., Poppe,B., Van Roy,N., De Paepe,A. and Speleman,F. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol., 18, RESEARCH 0034. Epub.
-
(2002)
Genome Biol.
, vol.18
-
-
Vandesompele, J.1
De Preter, K.2
Pattyn, F.3
Poppe, B.4
Van Roy, N.5
De Paepe, A.6
Speleman, F.7
-
14
-
-
25444494901
-
Quantification strategies in real-time PCR
-
Bustin,S.A. (ed.), International University Line, La Jolla, California
-
Pfaffl,M.W. (2004) Quantification strategies in real-time PCR. In Bustin,S.A. (ed.), A-Z of Quantitative PCR. International University Line, La Jolla, California, p.103.
-
(2004)
A-Z of Quantitative PCR
, pp. 103
-
-
Pfaffl, M.W.1
-
15
-
-
17344392308
-
A new mathematical model for relative quantification in real-time RT-PCR
-
Pfaffl,M.W. (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res., 29, e45.
-
(2001)
Nucleic Acids Res.
, vol.29
-
-
Pfaffl, M.W.1
-
16
-
-
1842829051
-
Real-time RT-PCR: Considerations for efficient and sensitive assay design
-
Peters,J.R., Helps,C.R., Hall,E.J. and Day,M.J. (2004) Real-time RT-PCR: considerations for efficient and sensitive assay design. J. Immunol. Methods, 286, 203-217.
-
(2004)
J. Immunol. Methods
, vol.286
, pp. 203-217
-
-
Peters, J.R.1
Helps, C.R.2
Hall, E.J.3
Day, M.J.4
-
17
-
-
0014964654
-
RNA-dependent DNA polymerase in virions of RNA tumour viruses
-
Baltimore,D. (1970) RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature, 226, 1209-1211.
-
(1970)
Nature
, vol.226
, pp. 1209-1211
-
-
Baltimore, D.1
-
18
-
-
0014964695
-
RNA-dependent DNA polymerase in virions of Rous sarcoma virus
-
Temin,H.M. and Mizutani,S. (1970) RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature, 226, 1211-1213.
-
(1970)
Nature
, vol.226
, pp. 1211-1213
-
-
Temin, H.M.1
Mizutani, S.2
-
19
-
-
0014933641
-
DNA-directed DNA polymerase activity in oncogenic RNA viruses
-
Spiegelman,S., Burny,A., Das,M.R., Keydar,J., Schlom,J., Travnicek,M. and Watson,K. (1970) DNA-directed DNA polymerase activity in oncogenic RNA viruses. Nature, 227, 1029-1031.
-
(1970)
Nature
, vol.227
, pp. 1029-1031
-
-
Spiegelman, S.1
Burny, A.2
Das, M.R.3
Keydar, J.4
Schlom, J.5
Travnicek, M.6
Watson, K.7
-
20
-
-
0014969826
-
-
Spiegelman,S., Burny,A., Das,M.R., Keydar,J., Schlom,J., Travnicek,M. and Watson,K. (1970) Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses. Nature, 228, 430-432.
-
(1970)
Nature
, vol.228
, pp. 430-432
-
-
Spiegelman, S.1
Burny, A.2
Das, M.R.3
Keydar, J.4
Schlom, J.5
Travnicek, M.6
Watson, K.7
-
21
-
-
0014969825
-
A DNA-dependent DNA polymerase and a DNA endonuclease in virions of Rous sarcoma virus
-
Mizutani,S., Boettiger,D. and Temin,H.M. (1970) A DNA-dependent DNA polymerase and a DNA endonuclease in virions of Rous sarcoma virus. Nature, 228, 424-427.
-
(1970)
Nature
, vol.228
, pp. 424-427
-
-
Mizutani, S.1
Boettiger, D.2
Temin, H.M.3
-
22
-
-
0019186230
-
RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus
-
Myers,J.C., Dobkin,C. and Spiegelman,S. (1980) RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus. Proc. Natl Acad. Sci. USA, 771, 316-1320.
-
(1980)
Proc. Natl Acad. Sci. USA
, vol.771
, pp. 316-1320
-
-
Myers, J.C.1
Dobkin, C.2
Spiegelman, S.3
-
23
-
-
0031028380
-
Conferring RNA polymerase activity to a DNA polymerase: A single residue in reverse transcriptase controls substrate selection
-
Gao,G., Orlova,M., Georgiadis,M.M., Hendrickson,W.A. and Goff,S.P. (1997) Conferring RNA polymerase activity to a DNA polymerase: a single residue in reverse transcriptase controls substrate selection. Proc. Natl Acad. Sci. USA, 94, 407-411.
-
(1997)
Proc. Natl Acad. Sci. USA
, vol.94
, pp. 407-411
-
-
Gao, G.1
Orlova, M.2
Georgiadis, M.M.3
Hendrickson, W.A.4
Goff, S.P.5
-
24
-
-
0038709523
-
Mutations proximal to the minor groove-binding track of human immunodeficiency virus type 1 reverse transcriptase differentially affect utilization of RNA versus DNA as template
-
Fisher,T.S., Darden,T. and Prasad,V.R. (2003) Mutations proximal to the minor groove-binding track of human immunodeficiency virus type 1 reverse transcriptase differentially affect utilization of RNA versus DNA as template. J Virol., 77, 5837-5845.
-
(2003)
J Virol.
, vol.77
, pp. 5837-5845
-
-
Fisher, T.S.1
Darden, T.2
Prasad, V.R.3
-
25
-
-
0035092560
-
Reverse transcriptase adds nontemplated nucleotides to cDNAs during 5′-RACE and primer extension
-
Chen,D. and Patton,J.T. (2001) Reverse transcriptase adds nontemplated nucleotides to cDNAs during 5′-RACE and primer extension. Biotechniques, 30, 574-580, 582.
-
(2001)
Biotechniques
, vol.30
, pp. 574-580
-
-
Chen, D.1
Patton, J.T.2
-
26
-
-
0029929520
-
Relationship between plus strand DNA synthesis removal of downstream segments of RNA by human immunodeficiency virus, murine leukemia virus and avian myeloblastoma virus reverse transcriptases
-
Fuentes,G.M., Fay,P.J. and Bambara,R.A. (1996) Relationship between plus strand DNA synthesis removal of downstream segments of RNA by human immunodeficiency virus, murine leukemia virus and avian myeloblastoma virus reverse transcriptases. Nucleic Acids Res., 24, 1719-1726.
-
(1996)
Nucleic Acids Res.
, vol.24
, pp. 1719-1726
-
-
Fuentes, G.M.1
Fay, P.J.2
Bambara, R.A.3
-
27
-
-
0031039590
-
Determination of the site of first strand transfer during Moloney murine leukemia virus reverse transcription and identification of strand transfer-associated reverse transcriptase errors
-
Kulpa,D., Topping,R. and Telesnitsky,A. (1997) Determination of the site of first strand transfer during Moloney murine leukemia virus reverse transcription and identification of strand transfer-associated reverse transcriptase errors. EMBO J., 16, 856-865.
-
(1997)
EMBO J.
, vol.16
, pp. 856-865
-
-
Kulpa, D.1
Topping, R.2
Telesnitsky, A.3
-
28
-
-
0032101286
-
Quantitative reverse transcription strand displacement amplification: Quantitation of nucleic acids using an isothermal amplification technique
-
Nycz,C.M., Dean,C.H., Haaland,P.D., Spargo,C.A. and Walker,G.T. (1998) Quantitative reverse transcription strand displacement amplification: quantitation of nucleic acids using an isothermal amplification technique. Anal. Biochem., 259, 226-234.
-
(1998)
Anal. Biochem.
, vol.259
, pp. 226-234
-
-
Nycz, C.M.1
Dean, C.H.2
Haaland, P.D.3
Spargo, C.A.4
Walker, G.T.5
-
29
-
-
0037100699
-
The role of template-primer in protection of reverse transcriptase from thermal inactivation
-
Gerard,G.F., Potter,R.J., Smith,M.D., Rosenthal,K., Dhariwal,G., Lee,J. and Chatterjee,D.K. (2002) The role of template-primer in protection of reverse transcriptase from thermal inactivation. Nucleic Acids Res., 30, 3118-3129.
-
(2002)
Nucleic Acids Res.
, vol.30
, pp. 3118-3129
-
-
Gerard, G.F.1
Potter, R.J.2
Smith, M.D.3
Rosenthal, K.4
Dhariwal, G.5
Lee, J.6
Chatterjee, D.K.7
-
30
-
-
0028901265
-
Continuous RT-PCR using AMV-RT and Taq DNA polymerase: Characterization and comparison to uncoupled procedures
-
Mallet,F., Oriol,G., Mary,C., Verrier,B. and Mandrand,B. (1995) Continuous RT-PCR using AMV-RT and Taq DNA polymerase: characterization and comparison to uncoupled procedures. Biotechniques, 18, 678-687.
-
(1995)
Biotechniques
, vol.18
, pp. 678-687
-
-
Mallet, F.1
Oriol, G.2
Mary, C.3
Verrier, B.4
Mandrand, B.5
-
31
-
-
0035987139
-
RNase H and its effects on PCR
-
Polumuri,S.K., Ruknudin,A. and Schulze,D.H. (2002) RNase H and its effects on PCR. Biotechniques, 32, 1224-1225.
-
(2002)
Biotechniques
, vol.32
, pp. 1224-1225
-
-
Polumuri, S.K.1
Ruknudin, A.2
Schulze, D.H.3
-
32
-
-
0033555647
-
Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR
-
Zhang,J. and Byrne,C.D. (1999) Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR. Biochem. J., 337, 231-241.
-
(1999)
Biochem. J.
, vol.337
, pp. 231-241
-
-
Zhang, J.1
Byrne, C.D.2
-
33
-
-
0033521113
-
Polypurine tract primer generation and utilization by Moloney murine leukemia virus reverse transcriptase
-
Schultz,S.J., Zhang,M., Kelleher,C.D. and Champoux,J.J. (1999) Polypurine tract primer generation and utilization by Moloney murine leukemia virus reverse transcriptase. J. Biol. Chem., 274, 34547-34555.
-
(1999)
J. Biol. Chem.
, vol.274
, pp. 34547-34555
-
-
Schultz, S.J.1
Zhang, M.2
Kelleher, C.D.3
Champoux, J.J.4
-
34
-
-
7044250957
-
The isolation of the components of streptococcal nucleoproteins in serologically active form
-
Sevag,M.G., Lackman,D.B. and Smolens,J. (1938) The isolation of the components of streptococcal nucleoproteins in serologically active form. Streptococcal Nucleoproteins, 425-436.
-
(1938)
Streptococcal Nucleoproteins
, pp. 425-436
-
-
Sevag, M.G.1
Lackman, D.B.2
Smolens, J.3
-
35
-
-
0002961944
-
Precipitation Techniques
-
Deutscher,M.P. (ed.), Academic Press, INC, San Diego, CA
-
England,S. and Seifter,S. (1990) Precipitation Techniques. In Deutscher,M.P. (ed.), Guide to Protein Purification. Methods in Eznymology. Academic Press, INC, San Diego, CA, pp. 296-298.
-
(1990)
Guide to Protein Purification. Methods in Eznymology
, pp. 296-298
-
-
England, S.1
Seifter, S.2
-
36
-
-
1542328972
-
Properties of the reverse transcription reaction in mRNA quantification
-
Epub
-
Stahlberg,A., Hakansson,J., Xian,X., Semb,H. and Kubista,M. (2004) Properties of the reverse transcription reaction in mRNA quantification. Clin. Chem., 50, 509-515. Epub.
-
(2004)
Clin. Chem.
, vol.50
, pp. 509-515
-
-
Stahlberg, A.1
Hakansson, J.2
Xian, X.3
Semb, H.4
Kubista, M.5
-
37
-
-
0037683339
-
Improved indicators for assessing the reliability of detection and quantification by kinetic PCR
-
Soong,R. and Ladanyi,A. (2003) Improved indicators for assessing the reliability of detection and quantification by kinetic PCR. Clin. Chem., 49, 973-976.
-
(2003)
Clin. Chem.
, vol.49
, pp. 973-976
-
-
Soong, R.1
Ladanyi, A.2
-
38
-
-
0025257556
-
Amplified RNA synthesized from limited quantities of heterogeneous cDNA
-
Van Gelder,R.N., von Zastrow,M.E., Yool,A., Dement,W.C., Barchas,J.D. and Eberwine,J.H. (1990) Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc. Natl Acad. Sci. USA, 87, 1663-1667.
-
(1990)
Proc. Natl Acad. Sci. USA
, vol.87
, pp. 1663-1667
-
-
Van Gelder, R.N.1
Von Zastrow, M.E.2
Yool, A.3
Dement, W.C.4
Barchas, J.D.5
Eberwine, J.H.6
-
39
-
-
0036793267
-
Yeast tRNA as carrier in the isolation of microscale RNA for global amplification and expression profiling
-
passim
-
Wang,Q.T., Xiao,W., Mindrinos,M. and Davis,R.W. (2002) Yeast tRNA as carrier in the isolation of microscale RNA for global amplification and expression profiling. Biotechniques, 33, 788, 790, 792 passim.
-
(2002)
Biotechniques
, vol.33
, pp. 788
-
-
Wang, Q.T.1
Xiao, W.2
Mindrinos, M.3
Davis, R.W.4
-
40
-
-
2442712711
-
Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling
-
Naderi,A., Ahmed,A.A., Barbosa-Morais,N.L., Aparicio,S., Brenton,J.D. and Caldas,C. (2004) Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling. BMC Genomics, 5, 9.
-
(2004)
BMC Genomics
, vol.5
, pp. 9
-
-
Naderi, A.1
Ahmed, A.A.2
Barbosa-Morais, N.L.3
Aparicio, S.4
Brenton, J.D.5
Caldas, C.6
-
41
-
-
0037195087
-
Neural stem cell heterogeneity demonstrated by molecular phenotyping of clonal neurospheres
-
Epub
-
Suslov,O.N., Kukekov,V.G., Ignatova,T.N. and Steindler,D.A. (2002) Neural stem cell heterogeneity demonstrated by molecular phenotyping of clonal neurospheres. Proc. Natl Acad. Sci. USA, 99, 14506-14511. Epub.
-
(2002)
Proc. Natl Acad. Sci. USA
, vol.99
, pp. 14506-14511
-
-
Suslov, O.N.1
Kukekov, V.G.2
Ignatova, T.N.3
Steindler, D.A.4
|