메뉴 건너뛰기




Volumn 5, Issue 4, 2005, Pages 493-498

Real-time, fluorescence-based quantitative PCR: A snapshot of current procedures and preferences

Author keywords

DNA; Fluorescence; PCR; Quantitative; Real time; Reverse transcripcase

Indexed keywords

NUCLEIC ACID;

EID: 22044458051     PISSN: 14737159     EISSN: None     Source Type: Journal    
DOI: 10.1586/14737159.5.4.493     Document Type: Review
Times cited : (114)

References (35)
  • 1
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25, 169-193 (2000).
    • (2000) J. Mol. Endocrinol. , vol.25 , pp. 169-193
    • Bustin, S.A.1
  • 2
    • 0036668567 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems
    • Bustin SA. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J. Mol. Endocrinol. 29, 23-39 (2002).
    • (2002) J. Mol. Endocrinol. , vol.29 , pp. 23-39
    • Bustin, S.A.1
  • 3
    • 0036278859 scopus 로고    scopus 로고
    • Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream
    • Ginzinger DG. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp. Hematol. 30, 503-512 (2002).
    • (2002) Exp. Hematol. , vol.30 , pp. 503-512
    • Ginzinger, D.G.1
  • 5
    • 0036724945 scopus 로고    scopus 로고
    • Improved quantitative real-time RT-PCR for expression profiling of individual cells
    • Liss B. Improved quantitative real-time RT-PCR for expression profiling of individual cells. Nucleic Acids Res. 30, E89 (2002).
    • (2002) Nucleic Acids Res. , vol.30
    • Liss, B.1
  • 6
    • 0035988627 scopus 로고    scopus 로고
    • Quantification using real-time PCR technology: Applications and limitations
    • Klein D. Quantification using real-time PCR technology: applications and limitations. Trends Mol. Med. 8, 257-260 (2002).
    • (2002) Trends Mol. Med. , vol.8 , pp. 257-260
    • Klein, D.1
  • 7
    • 1642318064 scopus 로고    scopus 로고
    • Real-time PCR in the microbiology laboratory
    • Mackay IM. Real-time PCR in the microbiology laboratory. Clin. Microbiol. Infect. 10, 190-212 (2004).
    • (2004) Clin. Microbiol. Infect. , vol.10 , pp. 190-212
    • Mackay, I.M.1
  • 8
    • 14044265394 scopus 로고    scopus 로고
    • Pitfalls of quantitative reverse transcription polymerase chain reaction
    • Bustin SA, Nolan T. Pitfalls of quantitative reverse transcription polymerase chain reaction. J. Biomol. Tech. 15, 155-166 (2004).
    • (2004) J. Biomol. Tech. , vol.15 , pp. 155-166
    • Bustin, S.A.1    Nolan, T.2
  • 9
    • 21344458602 scopus 로고    scopus 로고
    • Quantitative real-time reverse transcription polymerase chain reaction - A perspective
    • Bustin SA, Benes V, Nolan T, Pfaffl M. Quantitative real-time reverse transcription polymerase chain reaction - a perspective. J. Mol. Endocrinol. 34(3), 597-601 (2005).
    • (2005) J. Mol. Endocrinol. , vol.34 , Issue.3 , pp. 597-601
    • Bustin, S.A.1    Benes, V.2    Nolan, T.3    Pfaffl, M.4
  • 11
    • 16644361805 scopus 로고    scopus 로고
    • Intratumoral genomic heterogeneity in advanced head and neck cancer detected by comparative genomic hybridization
    • Gotte K, Tremmel SC, Popp S et al. Intratumoral genomic heterogeneity in advanced head and neck cancer detected by comparative genomic hybridization. Adv. Otorhinolaryngol. 62, 38-48 (2005).
    • (2005) Adv. Otorhinolaryngol. , vol.62 , pp. 38-48
    • Gotte, K.1    Tremmel, S.C.2    Popp, S.3
  • 12
    • 19844364343 scopus 로고    scopus 로고
    • Evolution of intratumoral genetic heterogeneity during colorectal cancer progression
    • Losi L, Baisse B, Bouzourene H, Benhattar J. Evolution of intratumoral genetic heterogeneity during colorectal cancer progression. Carcinogenesis 26, 916-922 (2005).
    • (2005) Carcinogenesis , vol.26 , pp. 916-922
    • Losi, L.1    Baisse, B.2    Bouzourene, H.3    Benhattar, J.4
  • 13
    • 0036828286 scopus 로고    scopus 로고
    • Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling
    • Wang W, Wyckoff JB, Frohlich VC et al. Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Res. 62, 6278-6288 (2002).
    • (2002) Cancer Res. , vol.62 , pp. 6278-6288
    • Wang, W.1    Wyckoff, J.B.2    Frohlich, V.C.3
  • 14
    • 6344291988 scopus 로고    scopus 로고
    • Quantification of multiple gene expression in individual cells
    • Peixoto A, Monteiro M, Rocha B, Veiga-Fernandes H. Quantification of multiple gene expression in individual cells. Genome Res. 14, 1938-1947 (2004).
    • (2004) Genome Res. , vol.14 , pp. 1938-1947
    • Peixoto, A.1    Monteiro, M.2    Rocha, B.3    Veiga-Fernandes, H.4
  • 15
    • 0035559257 scopus 로고    scopus 로고
    • Clinical utility of viral quantification as a tool for disease monitoring
    • Schutten M, Niesters HG. Clinical utility of viral quantification as a tool for disease monitoring. Expert Rev. Mol. Diagn. 1, 153-162 (2001).
    • (2001) Expert Rev. Mol. Diagn. , vol.1 , pp. 153-162
    • Schutten, M.1    Niesters, H.G.2
  • 16
    • 9144222001 scopus 로고    scopus 로고
    • Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia - A Europe Against Cancer program
    • Gabert J, Beillard E, van der Velden VH et al. Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia - a Europe Against Cancer program. Leukemia 17, 2318-2357 (2003).
    • (2003) Leukemia , vol.17 , pp. 2318-2357
    • Gabert, J.1    Beillard, E.2    Van Der Velden, V.H.3
  • 17
    • 2642516188 scopus 로고    scopus 로고
    • Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia
    • Muller MC, Hordt T, Paschka P et al. Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia. Acta Haematol. 112, 30-33 (2004).
    • (2004) Acta Haematol. , vol.112 , pp. 30-33
    • Muller, M.C.1    Hordt, T.2    Paschka, P.3
  • 20
    • 4344564046 scopus 로고    scopus 로고
    • Comparison of reverse transcriptases in gene expression analysis
    • Stahlberg A, Kubista M, Pfaffl M. Comparison of reverse transcriptases in gene expression analysis. Clin. Chem. 50, 1678-1680 (2004).
    • (2004) Clin. Chem. , vol.50 , pp. 1678-1680
    • Stahlberg, A.1    Kubista, M.2    Pfaffl, M.3
  • 21
    • 21844467196 scopus 로고    scopus 로고
    • Comparison of amplification enzymes for hepatitis C virus quasispecies analysis
    • Polyak SJ, Sullivan DG, Austin MA et al. Comparison of amplification enzymes for hepatitis C virus quasispecies analysis. Virol. J. 2, 41 (2005).
    • (2005) Virol. J. , vol.2 , pp. 41
    • Polyak, S.J.1    Sullivan, D.G.2    Austin, M.A.3
  • 22
    • 1542328972 scopus 로고    scopus 로고
    • Properties of the reverse transcription reaction in mRNA quantification
    • Stahlberg A, Hakansson J, Xian X, Semb H, Kubista M. Properties of the reverse transcription reaction in mRNA quantification. Clin. Chem. 50, 509-515 (2004).
    • (2004) Clin. Chem. , vol.50 , pp. 509-515
    • Stahlberg, A.1    Hakansson, J.2    Xian, X.3    Semb, H.4    Kubista, M.5
  • 23
    • 22044443786 scopus 로고    scopus 로고
    • Basic RT-PCR considerations
    • Bustin S (Ed.), IUL Press, CA, USA
    • Bustin S, Nolan T. Basic RT-PCR considerations. In: A-Z of Quantitative PCR. Bustin S (Ed.), IUL Press, CA, USA, 359-396 (2004).
    • (2004) A-Z of Quantitative PCR , pp. 359-396
    • Bustin, S.1    Nolan, T.2
  • 24
    • 0036499301 scopus 로고    scopus 로고
    • A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics
    • Liu W, Saint DA. A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics. Anal. Biochem. 302, 52-59 (2002).
    • (2002) Anal. Biochem. , vol.302 , pp. 52-59
    • Liu, W.1    Saint, D.A.2
  • 25
    • 0037109753 scopus 로고    scopus 로고
    • Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
    • Tricarico C, Pinzani P, Bianchi S et al. Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal. Biochem. 309, 293-300 (2002).
    • (2002) Anal. Biochem. , vol.309 , pp. 293-300
    • Tricarico, C.1    Pinzani, P.2    Bianchi, S.3
  • 26
    • 2942700135 scopus 로고    scopus 로고
    • Utility of the housekeeping genes 18S rRNA, β-actin and glyceraldehyde-3-phosphate-dehydrogenase for normalization in real-time quantitative reverse transcriptase-polymerase chain reaction analysis of gene expression in human T-lymphocytes
    • Bas A, Forsberg G, Hammarstrom S, Hammarstrom ML. Utility of the housekeeping genes 18S rRNA, β-actin and glyceraldehyde-3-phosphate- dehydrogenase for normalization in real-time quantitative reverse transcriptase-polymerase chain reaction analysis of gene expression in human T-lymphocytes. Scand. J. Immunol. 59, 566-573 (2004).
    • (2004) Scand. J. Immunol. , vol.59 , pp. 566-573
    • Bas, A.1    Forsberg, G.2    Hammarstrom, S.3    Hammarstrom, M.L.4
  • 27
    • 3442876969 scopus 로고    scopus 로고
    • Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    • Andersen CL, Jensen JL, Orntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64, 5245-5250 (2004).
    • (2004) Cancer Res. , vol.64 , pp. 5245-5250
    • Andersen, C.L.1    Jensen, J.L.2    Orntoft, T.F.3
  • 28
    • 0037227141 scopus 로고    scopus 로고
    • Exogenous reference RNA for normalization of real-time quantitative PCR
    • Smith RD, Brown B, Ikonomi P, Schechter AN. Exogenous reference RNA for normalization of real-time quantitative PCR. Biotechniques 34, 88-91 (2003).
    • (2003) Biotechniques , vol.34 , pp. 88-91
    • Smith, R.D.1    Brown, B.2    Ikonomi, P.3    Schechter, A.N.4
  • 29
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • Vandesompele J, De Preter K, Pattyn F et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3, 0034.1-0034.11 (2002).
    • (2002) Genome Biol. , vol.3
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3
  • 30
    • 0036581160 scopus 로고    scopus 로고
    • Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
    • Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 30, e36 (2002).
    • (2002) Nucleic Acids Res. , vol.30
    • Pfaffl, M.W.1    Horgan, G.W.2    Dempfle, L.3
  • 31
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time RT-PCR
    • Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29, E45 (2001).
    • (2001) Nucleic Acids Res. , vol.29
    • Pfaffl, M.W.1
  • 32
    • 3142615272 scopus 로고    scopus 로고
    • Validation of housekeeping genes for normalizing RNA expression in real-time PCR
    • Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques 37, 112-114, 116, 118-119 (2004).
    • (2004) Biotechniques , vol.37 , pp. 112-114
    • Dheda, K.1    Huggett, J.F.2    Bustin, S.A.3    Johnson, M.A.4    Rook, G.5    Zumla, A.6
  • 33
    • 21344471138 scopus 로고    scopus 로고
    • Real-time RT-PCR normalisation; strategies and considerations
    • Huggett J, Dheda K, Bustin S, Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 6(4), 279-284 (2005).
    • (2005) Genes Immun. , vol.6 , Issue.4 , pp. 279-284
    • Huggett, J.1    Dheda, K.2    Bustin, S.3    Zumla, A.4
  • 35
    • 0038824761 scopus 로고    scopus 로고
    • Customized molecular phenotyping by quantitative gene expression and pattern recognition analysis
    • Akilesh S, Shaffer DJ, Roopenian D. Customized molecular phenotyping by quantitative gene expression and pattern recognition analysis. Genome Res. 13, 1719-1727 (2003).
    • (2003) Genome Res. , vol.13 , pp. 1719-1727
    • Akilesh, S.1    Shaffer, D.J.2    Roopenian, D.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.