Drug-target identification in Drosophila cells: combining high-throughout RNAi and small-molecule screens

Drug Discovery Today

Volumn 12, Issue 1-2, 2007, Pages 28-33

  Perrimon, Norbert ^a   Friedman, Adam ^a   Mathey Prevot, Bernard ^a   Eggert, Ulrike S ^b  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHOTRANSFERASE INHIBITOR;

CYTOKINESIS; DROSOPHILA; DRUG DETERMINATION; DRUG EFFECT; DRUG RESEARCH; DRUG TARGETING; GENOMICS; HIGH THROUGHPUT SCREENING; MEDICAL SCHOOL; MOLECULAR SIZE; NONHUMAN; PHARMACOGENETICS; REVIEW; RNA INTERFERENCE;

ANIMALS; DROSOPHILA; DRUG DELIVERY SYSTEMS; DRUG DESIGN; DRUG EVALUATION, PRECLINICAL; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 33845769956     PISSN: 13596446     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.drudis.2006.10.006     Document Type: Review

References (39)

1. Eggert U.S., et al. Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets. PLoS Biol 2 (2004) e379
2. Hendricks J.C., et al. Modafinil maintains waking in the fruit fly Drosophila melanogaster. Sleep 26 (2003) 139-146
3. Marsh J.L., and Thompson L.M. Can flies help humans treat neurodegenerative diseases?. Bioessays 26 (2004) 485-496
4. Zhang X., et al. A potent small molecule inhibits polyglutamine aggregation in Huntington's disease neurons and suppresses neurodegeneration in vivo. Proc Natl Acad Sci U S A. 102 (2005) 892-897
5. Steffan J.S., et al. Histone deacetylase inhibitors arrest polyglutamine-dependent neurodegeneration in Drosophila. Nature 413 (2001) 739-743
6. Micchelli C.A., et al. Gamma-secretase/presenilin inhibitors for Alzheimer's disease phenocopy Notch mutations in Drosophila. Faseb J 17 (2003) 79-81
7. Rubin G.M., et al. Comparative genomics of the eukaryotes. Science 287 (2000) 2204-2215
8. Bier E. Drosophila, the golden bug, emerges as a tool for human genetics. Nat. Rev. Genet. 6 (2005) 9-23
9. Brumby A.M., and Richardson H.E. Using Drosophila melanogaster to map human cancer pathways. Nat. Rev. Cancer 5 (2005) 626-639
10. Carlomagno F., et al. ZD6474, an orally available inhibitor of KDR tyrosine kinase activity, efficiently blocks oncogenic RET kinases. Cancer Res. 62 (2002) 7284-7290
11. Vidal M., et al. ZD6474 suppresses oncogenic RET isoforms in a Drosophila model for type 2 multiple endocrine neoplasia syndromes and papillary thyroid carcinoma. Cancer Res. 65 (2005) 3538-3541
12. Spring D.R. Chemical genetics to chemical genomics: small molecules offer big insights. Chem. Soc. Rev. 34 (2005) 472-482
13. Eggert U.S., and Mitchison T.J. Small molecule screening by imaging. Curr. Opin. Chem. Biol. 10 (2006) 232-237
14. Adams M.D., et al. The genome sequence of Drosophila melanogaster. Science 287 (2000) 2185-2195
15. Boutros M., et al. Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science 303 (2004) 832-835
16. Kiger A., et al. A functional genomic analysis of cell morphology using RNA interference. J. Biol. 2 (2003) 27
17. Armknecht S., et al. High-throughput RNA interference screens in Drosophila tissue culture cells. Methods Enzymol. 392 (2005) 55-73
18. Clemens J.C., et al. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc. Natl. Acad. Sci. U. S. A. 97 (2000) 6499-6503
19. Baeg G.H., et al. N. Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila. Genes Dev. 19 (2005) 1861-1870
20. DasGupta R., et al. Functional Genomic Analysis of the Wnt-Wingless Signaling Pathway. Science 308 (2005) 826-833
21. Lum L., et al. Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells. Science 299 (2003) 2039-2045
22. Nybakken K., et al. A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nat Genet. 37 (2005) 1323-1332
23. Muller P., et al. Identification of JAK/STAT signalling components by genome-wide RNA interference. Nature 436 (2005) 871-875
24. Cherry S., et al. Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition. Genes Dev. 19 (2005) 445-452
25. Bjorklund M., et al. Identification of pathways regulating cell size and cell-cycle progression by RNAi. Nature 439 (2006) 1009-1013
26. Agaisse H., et al. Genome-wide RNAi screen for host factors required for intracellular bacterial infection. Science. 309 (2005) 1248-1251
27. Feske S., et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature. 441 (2006) 179-185
28. Philips J.A., et al. Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection. Science. 309 (2005) 1251-1253
29. Gwack Y., et al. A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT. Nature. 441 (2006) 646-650
30. Carpenter A.E., and Sabatini D.M. Systematic genome-wide screens of gene function. Nat. Rev. Genet. 5 (2004) 11-22
31. Echeverri C.J., and Perrimon N. High-throughput RNAi screening in cultured cells: a user's guide. Nat. Rev. Genet. 7 (2006) 373-384
32. Ramet M., et al. Functional genomic analysis of phagocytosis and identification of a Drosophila receptor for E. coli. Nature 416 (2002) 644-648
33. Vig M., et al. CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Science. 312 (2006) 1220-1223
34. Zhang S.L., et al. Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity. Proc. Natl. Acad. Sci. U. S. A. 103 (2006) 9357-9362
35. Friedman A., and Perrimon N. Genome-wide high-throughput screens in functional genomics. Curr. Opin. Genet. Dev. 14 (2004) 470-476
36. Fishman M.C., and Porter J.A. Pharmaceuticals: a new grammar for drug discovery. Nature 437 (2005) 491-493
37. Guertin D.A., et al. Functional genomics identifies TOR-regulated genes that control growth and division. Curr. Biol. 16 (2006) 958-970
38. Hoffmann J.A. The immune response of Drosophila. Nature 426 (2003) 33-38
39. Kulkarni M.M., and Perrimon N. Super-size flies. Cell Metab. 1 (2005) 288-290