Genome-wide RNAi screen for host factors required for intracellular bacterial infection

Science

Volumn 309, Issue 5738, 2005, Pages 1248-1251

  Agaisse, Hervé ^a,c   Burrack, Laura S ^a   Philips, Jennifer A ^a   Rubin, Eric J ^b   Perrimon, Norbert ^a   Higgins, Darren E ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

^c YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; ENVIRONMENTAL IMPACT; GENETIC ENGINEERING; MICROBIOLOGY;

BACTERIAL PATHOGENESIS; DROSOPHILA CELLS; MONOCYTOGENES; MYCOBACTERUM;

RNA;

DOUBLE STRANDED RNA;

RNA;

ARTICLE; BACTERIAL INFECTION; BACTERIAL VIRULENCE; CELL FUNCTION; CELL VACUOLE; CYTOSOL; DROSOPHILA; GENOME ANALYSIS; HOST PATHOGEN INTERACTION; LISTERIA MONOCYTOGENES; MYCOBACTERIUM FORTUITUM; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; RNA INTERFERENCE;

ANIMALS; CELL CYCLE; CELL LINE; CYTOSKELETON; CYTOSOL; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; GENES, INSECT; GENOME; GREEN FLUORESCENT PROTEINS; LISTERIA MONOCYTOGENES; MACROPHAGES; MYCOBACTERIUM FORTUITUM; PHENOTYPE; RNA INTERFERENCE; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, DOUBLE-STRANDED; SIGNAL TRANSDUCTION; VACUOLES; VESICULAR TRANSPORT PROTEINS;

LISTERIA MONOCYTOGENES; MYCOBACTERIUM FORTUITUM;

EID: 23844442475     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1116008     Document Type: Article

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16. We are grateful to the Drosophila RNAi Screening Center for providing access to the dsRNA library and screening facilities. We thank members of the Perrimon lab and Higgins lab for assistance and support, especially A. Shen for construction of strain DHL1039. This work was supported by grant AI053669 (D.E.H.) and AI048704 (E.J.R.) from the NIH. N.P. is an Investigator of the Howard Hughes Medical Institute (HHMI). Work in the Perrimon lab was supported by HHMI and the NIH. L.S.B. is a HHMI Predoctoral Fellow.