Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection

Science

Volumn 309, Issue 5738, 2005, Pages 1251-1253

  Philips, Jennifer A ^a   Rubin, Eric J ^c   Perrimon, Norbert ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^c HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELLS; ESCHERICHIA COLI; GENETIC ENGINEERING;

MACROPHAGE; MAMMALIAN CLASS B; MYCOBACTERIAL INFECTION; PATHOGENS;

RNA;

CD36 ANTIGEN; PROTEIN PESTE; SCAVENGER RECEPTOR; UNCLASSIFIED DRUG;

RNA;

ARTICLE; DROSOPHILA; ENVIRONMENTAL FACTOR; ESCHERICHIA COLI; GENETIC CONSERVATION; GENOME ANALYSIS; HUMAN; HUMAN CELL; IMMUNITY; MACROPHAGE; MAMMAL; MOLECULAR RECOGNITION; MULTIGENE FAMILY; MYCOBACTERIOSIS; MYCOBACTERIUM FORTUITUM; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PHAGOCYTOSIS; PRIORITY JOURNAL; RNA INTERFERENCE; STAPHYLOCOCCUS AUREUS; SURVIVAL;

ANIMALS; ANTIGENS, CD36; CELL LINE; CYTOSKELETON; DROSOPHILA MELANOGASTER; ESCHERICHIA COLI; HUMANS; IMMUNITY, NATURAL; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEINS; MACROPHAGES; MEMBRANE PROTEINS; MICE; MYCOBACTERIUM FORTUITUM; PHAGOCYTOSIS; RECEPTORS, IMMUNOLOGIC; RECEPTORS, SCAVENGER; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; SCAVENGER RECEPTORS, CLASS B; SIALOGLYCOPROTEINS; STAPHYLOCOCCUS AUREUS; TRANSFECTION; TRANSPORT VESICLES;

CORYNEBACTERINEAE; ESCHERICHIA COLI; MAMMALIA; MYCOBACTERIUM FORTUITUM; MYCOBACTERIUM TUBERCULOSIS; STAPHYLOCOCCUS AUREUS;

EID: 23844531867     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1116006     Document Type: Article

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22. We thank C. Cosma and L. Ramikrishnan (University of Washington) for providing map24 and map49 plasmids. Expression constructs for CD36, SR-BI and SR-BII, and LIMP-II were provided by K. Moore (Massachusetts General Hospital), J. Owen (University College London), and K. Akasaki (Fukuyama University), respectively. S. De Bruin and N. Gliksman (Universal Imaging) provided MetaMorph software training, E. Troy, L. Burrack, and D. Higgins assisted with bone marrow-derived macrophages experiments. We are indebted to the Drosophila RNAi Screening Center for providing expertise and the facilities for screening, and to the Perrimon and Rubin laboratory members for assistance, in particular S. Cherry, D. Higgins, B. Mathey-Prevot, L. Burrack, and M. Gibson provided useful comments on the manuscript. This work was supported by the NIH. N.P. is an Investigator of the Howard Hughes Medical Institute.