Fused protein of δPKC activation loop and PDK1-interacting fragment (δAL-PIF) functions as a pseudosubstrate and an inhibitory molecule for PDK1 when expressed in cells

Genes to Cells

Volumn 11, Issue 9, 2006, Pages 1051-1070

  Seki, Takahiro ^a   Irie, Naoki ^a,e   Nakamura, Kyoko ^c   Sakaue, Hiroshi ^c   Ogawa, Wataru ^c   Kasuga, Masato ^c   Yamamoto, Hideyuki ^d   Ohmori, Shiho ^b   Saito, Naoaki ^b   Sakai, Norio ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

^b KOBE UNIVERSITY   (Japan)

^c KOBE UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^d KUMAMOTO UNIVERSITY   (Japan)

^e KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CELL PROTEIN; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; PROTEIN KINASE C DELTA; PROTEIN KINASE C GAMMA; PROTEIN PDK1; PROTEIN SUBUNIT; THREONINE; UNCLASSIFIED DRUG; 3 PHOSPHOINOSITIDE DEPENDENT PROTEIN KINASE; 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE; ADENOSINE TRIPHOSPHATE; GLUTAMIC ACID; PHOSPHOTHREONINE; PROTEIN KINASE C; PROTEIN SERINE THREONINE KINASE;

ANIMAL CELL; ANTIBODY SPECIFICITY; APOPTOSIS; ARTICLE; CANCER THERAPY; CELL CULTURE; CELL FUNCTION; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVATION; GENE CONSTRUCT; KNOCKOUT GENE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION; ANIMAL; CELL STRAIN COS1; CERCOPITHECUS; CHEMISTRY; CHO CELL; CRICETULUS; DRUG EFFECT; GENE EXPRESSION; HAMSTER; METABOLISM; MOUSE MUTANT; PHOSPHORYLATION; PROTEIN BINDING; RAT;

ADENOSINE TRIPHOSPHATE; ANIMALS; APOPTOSIS; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; CHO CELLS; COS CELLS; CRICETINAE; CRICETULUS; ENZYME ACTIVATION; GENE EXPRESSION; GLUTAMIC ACID; MICE; MICE, KNOCKOUT; PHOSPHORYLATION; PHOSPHOTHREONINE; PROTEIN BINDING; PROTEIN KINASE C; PROTEIN KINASE C-DELTA; PROTEIN TRANSPORT; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS C-AKT; RATS; RECOMBINANT FUSION PROTEINS;

EID: 33747478593     PISSN: 13569597     EISSN: 13652443     Source Type: Journal    
DOI: 10.1111/j.1365-2443.2006.01003.x     Document Type: Article

References (51)

1. Alessi, D.R. (2001) Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture. Biochem. Soc. Trans. 29, 1-14.
2. Alessi, D.R., Deak, M., Casamayor, A., Caudwell, F.B., Morrice, N., Norman, D.G., Gaffney, P., Reese, C.B., MacDougall, C.N., Harbison, D., Ashworth, A. & Bownes, M. (1997a) 3-Phosphoinositide-dependent protein kinase-1 (PDK1): Structural and functional homology with the Drosophila DSTPK61 kinase. Curr. Biol. 7, 776-789.
3. Alessi, D.R., James, S.R., Downes, C.P., Holmes, A.B., Gaffney, P.R., Reese, C.B. & Cohen, P. (1997b) Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Bα. Curr. Biol. 7, 261-269.
4. Arico, S., Pattingre, S., Bauvy, C., Gane, P., Barbat, A., Codogno, P. & Ogier-Denis, E. (2002) Celecoxib induces apoptosis by inhibiting 3-phosphoinositide-dependent protein kinase-1 activity in the human colon cancer HT-29 cell line. J. Biol. Chem. 277, 27613-27621.
5. Balendran, A., Casamayor, A., Deak, M., Paterson, A., Gaffney, P., Currie, R., Downes, C.P. & Alessi, D.R. (1999) PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2. Curr. Biol. 9, 393-404.
6. Balendran, A., Hare, G.R., Kieloch, A., Williams, M.R. & Alessi, D.R. (2000) Further evidence that 3-phosphoinositide-dependent protein kinase-1 (PDK1) is required for the stability and phosphorylation of protein kinase C (PKC) isoforms. FEBS Lett. 484, 217-223.
7. Ballif, B.A., Shimamura, A., Pae, E. & Blenis, J. (2001) Disruption of 3-phosphoinositide-dependent kinase 1 (PDK1) signaling by the anti-tumorigenic and anti-proliferative agent N-α-tosyl-L-phenylalanyl chloromethyl ketone. J. Biol. Chem. 276, 12466-12475.
8. Behn-Krappa, A. & Newton, A.C. (1999) The hydrophobic phosphorylation motif of conventional protein kinase C is regulated by autophosphorylation. Curr. Biol. 9, 728-737.
9. Belham, C., Wu, S. & Avruch, J. (1999) Intracellular signalling: PDK1 - a kinase at the hub of things. Curr. Biol. 9, R93-R96.
10. Biondi, R.M., Cheung, P.C.F., Casamayor, A., Deak, M., Currie, R.A. & Alessi, D.R. (2000) Identification of a pocket in the PDK1 kinase domain that interacts with PIF and the C-terminal residues of PKA. EMBO J. 19, 979-988.
11. Blackshear, P.J. (1993) The MARCKS family of cellular protein kinase C substrates. J. Biol. Chem. 268, 1501-1504.
12. Bornancin, F. & Parker, P.J. (1996) Phosphorylation of threonine 638 critically controls the dephosphorylation and inactivation of protein kinase Cα. Curr. Biol. 6, 1114-1123.
13. Bronisz, A., Gajkowska, B. & Domanska-Janik, K. (2002) PKC and Raf-1 inhibition-related apoptotic signalling in N2a cells. J. Neurochem. 81, 1176-1184.
14. Collins, B.J., Deak, M., Arthur, J.S., Armit, L.J. & Alessi, D.R. (2003) In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation. EMBO J. 22, 4202-4211.
15. Downward, J. (1998) Mechanisms and consequences of activation of protein kinase B/Akt. Curr. Opin. Cell Biol. 10, 262-267.
16. Edwards, A.S. & Newton, A.C. (1997) Phosphorylation at conserved carboxyl-terminal hydrophobic motif regulates the catalytic and regulatory domains of protein kinase C. J. Biol. Chem. 272, 18382-18390.
17. Fresno Vara, J.A., Casado, E., de Castro, J., Cejas, P., Belda-Iniesta, C. & Gonzalez-Baron, M. (2004) PI3K/Akt signalling pathway and cancer. Cancer. Treat. Rev. 30, 193-204.
18. Gao, T., Toker, A. & Newton, A.C. (2001) The carboxyl terminus of protein kinase c provides a switch to regulate its interaction with the phosphoinositide-dependent kinase, PDK-1. J. Biol. Chem. 276, 19588-19596.
19. Hinton, H.J., Alessi, D.R. & Cantrell, D.A. (2004) The serine kinase phosphoinositide-dependent kinase 1 (PDK1) regulates T cell development. Nat. Immunol. 5, 539-545.
20. Inoue, H., Ogawa, W., Asakawa, A. et al. (2006) Role of hepatic STAT3 in brain-insulin action on hepatic glucose production. Cell. Metab. 3, 267-275.
21. Jensen, C.J., Buch, M.B., Krag, T.O., Hemmings, B.A., Gammeltoft, S. & Frodin, M. (1999) 90-kDa ribosomal S6 kinase is phosphorylated and activated by 3-phosphoinositide-dependent protein kinase-1. J. Biol. Chem. 274, 27168-27176.
22. Kim, D.W., Hwang, J.H., Suh, J.M., Kim, H., Song, J.H., Hwang, E.S., Hwang, I.Y., Park, K.C., Chung, H.K., Kim, J.M., Park, J., Hemmings, B.A. & Shong, M. (2003) RET/PTC (rearranged in transformation/ papillary thyroid carcinomas) tyrosine kinase phosphorylates and activates phosphoinositide-dependent kinase 1 (PDK1): An alternative phosphatidylinositol 3-kinase-independent pathway to activate PDK1. Mol. Endocrinol. 17, 1382-1394.
23. Kobayashi, T. & Cohen, P. (1999) Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2. Biochem. J. 339 (2), 319-328.
24. Lawlor, M.A., Mora, A., Ashby, P.R., Williams, M.R., Murray-Tait, V., Malone, L., Prescott, A.R., Lucocq, J.M. & Alessi, D.R. (2002) Essential role of PDK1 in regulating cell size and development in mice. EMBO J. 21, 3728-3738.
25. Le Good, J.A., Ziegler, W.H., Parekh, D.B., Alessi, D.R., Cohen, P. & Parker, P.J. (1998) Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1. Science 281, 2042-2045.
26. McManus, E.J., Collins, B.J., Ashby, P.R., Prescott, A.R., Murray-Tait, V., Armit, L.J., Arthur, J.S. & Alessi, D.R. (2004) The in vivo role of PtdIns (3,4,5) P (3) binding to PDK1 PH domain defined by knockin mutation. EMBO J. 23, 2071-2082.
27. Mora, A., Davies, A.M., Bertrand, L., Sharif, I., Budas, G.R., Jovanovic, S., Mouton, V., Kahn, C.R., Lucocq, J.M., Gray, G.A., Jovanovic, A. & Alessi, D.R. (2003) Deficiency of PDK1 in cardiac muscle results in heart failure and increased sensitivity to hypoxia. EMBO J. 22, 4666-4676.
28. Mora, A., Komander, D., van Aalten, D.M. & Alessi, D.R. (2004) PDK1, the master regulator of AGC kinase signal transduction. Semin. Cell Dev. Biol. 15, 161-170.
29. Mora, A., Lipina, C., Tronche, F., Sutherland, C. & Alessi, D.R. (2005) Deficiency of PDK1 in liver results in glucose intolerance, impairment of insulin-regulated gene expression and liver failure. Biochem. J. 385, 639-648.
30. Oancea, E. & Meyer, T. (1998) Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals. Cell 95, 307-318.
31. Ohmori, S., Sakai, N., Shirai, Y., Yamamoto, H., Miyamoto, E., Shimizu, N. & Saito, N. (2000) Importance of protein kinase C targeting for the phosphorylation of its substrate, myristoylated alanine-rich C-kinase substrate. J. Biol. Chem. 275, 26449-26457.
32. Ohmori, S., Shirai, Y., Sakai, N., Fujii, M., Konishi, H., Kikkawa, U. & Saito, N. (1998) Three distinct mechanisms for translocation and activation of the delta subspecies of protein kinase C. Mol. Cell. Biol. 18, 5263-5271.
33. Ono, Y., Fujii, T., Igarashi, K., Kikkawa, U., Ogita, K. & Nishizuka, Y. (1988) Nucleotide sequences of cDNAs for alpha and gamma subspecies of rat brain protein kinase C. Nucleic Acids Res. 16, 5199-5200.
34. Osaki, M., Oshimura, M. & Ito, H. (2004) PI3K-Akt pathway: Its functions and alterations in human cancer. Apoptosis 9, 667-676.
35. Pullen, N., Dennis, P.B., Andjelkovic, M., Dufner, A., Kozma, S.C., Hemmings, B.A. & Thomas, G. (1998) Phosphorylation and activation of p70s6k by PDK1. Science 279, 707-710.
36. Ruvolo, P.P., Deng, X., Carr, B.K. & May, W.S. (1998) A functional role for mitochondrial protein kinase Cα in Bcl2 phosphorylation and suppression of apoptosis. J. Biol. Chem. 273, 25436-25442.
37. Sakai, N., Sasaki, K., Ikegaki, N., Shirai, Y., Ono, Y. & Saito, N. (1997) Direct visualization of the translocation of the gamma-subspecies of protein kinase C in living cells using fusion proteins with green fluorescent protein. J. Cell Biol. 139, 1465-1476.
38. Sakai, N., Tsubokawa, H., Matsuzaki, M., Kajimoto, T., Takahashi, E., Ren, Y., Ohmori, S., Shirai, Y., Matsubayashi, H., Chen, J., Duman, R.S., Kasai, H. & Saito, N. (2004) Propagation of γPKC translocation along the dendrites of Purkinje cell in γPKC-GFP transgenic mice. Genes Cells 9, 945-957.
39. Sakaue, H., Nishizawa, A., Ogawa, W., Teshigawara, K., Mori, T., Takashima, Y., Noda, T. & Kasuga, M. (2003) Requirement for 3-phosphoinositide-kependent dinase-1 (PDK-1) in insulin-induced glucose uptake in immortalized brown adipocytes. J. Biol. Chem. 278, 38870-38874.
40. Sato, S., Fujita, N. & Tsuruo, T. (2002) Interference with PDK1-Akt survival signaling pathway by UCN-01 (7-hydroxystaurosporine). Oncogene 21, 1727-1738.
41. Seki, T., Matsubayashi, H., Amano, T., Shirai, Y., Saito, N. & Sakai, N. (2005) Phosphorylation of PKC activation loop plays an important role in receptor-mediated translocation of PKC. Genes Cells 10, 225-239.
42. Shirai, Y., Kashiwagi, K., Sakai, N. & Saito, N. (2000) Phospholipase A (2) and its products are involved in the purinergic receptor-mediated translocation of protein kinase C in CHO-K1 cells. J. Cell Sci. 113, 1335-1343.
43. Shirai, Y., Sakai, N. & Saito, N. (1998) Subspecies-specific targeting mechanism of protein kinase C. Jpn. J. Pharmacol. 78, 411-417.
44. Sonnenburg, E.D., Gao, T. & Newton, A.C. (2001) The phosphoinositide-dependent kinase, PDK-1, phosphorylates conventional protein kinase C isozymes by a mechanism that is independent of phosphoinositide 3-kinase. J. Biol. Chem. 276, 45289-45297.
45. Stempka, L., Girod, A., Muller, H.J., Rincke, G., Marks, F., Gschwendt, M. & Bossemeyer, D. (1997) Phosphorylation of protein kinase Cdelta (PKCdelta) at threonine 505 is not a prerequisite for enzymatic activity. Expression of rat PKCdelta and an alanine 505 mutant in bacteria in a functional form. J. Biol. Chem. 272, 6805-6811.
46. Stephens, L., Anderson, K., Stokoe, D., Erdjument-Bromage, H., Painter, G.F., Holmes, A.B., Gaffney, P.R., Reese, C.B., McCormick, F., Tempst, P., Coadwell, J. & Hawkins, P.T. (1998) Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B. Science 279, 710-714.
47. Todaro, G.J. & Green, H. (1963) Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17, 299-313.
48. Toker, A. & Newton, A.C. (2000) Cellular signaling: Pivoting around PDK-1. Cell 103, 185-188.
49. Vanhaesebroeck, B. & Alessi, D.R. (2000) The PI3K-PDK1 connection: more than just a road to PKB. Biochem. J. 346, 561-576.
50. Williams, M.R., Arthur, J.S.C., Balendran, A., van der Kaay, J., Poli, V., Cohen, P. & Alessi, D.R. (2000) The role of 3-phosphoinositide-dependent kinase 1 in activating AGC kinases defined in embryonic stem cells. Curr. Biol. 10, 439-448.
51. Yamamoto, H., Matsumura, T., Kugiyama, K., Oishi, Y., Ogata, N., Yasue, H. & Miyamoto, E. (1998) The antibody specific for myristoylated alanine-rich C kinase substrate phosphorylated by protein kinase C: activation of protein kinase C in smooth muscle cells in human coronary arteries. Arch. Biochem. Biophys. 359, 151-159.