Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Bα

Current Biology

Volumn 7, Issue 4, 1997, Pages 261-269

  Alessi, Dario R ^a   James, Stephen R ^a   Downes, C Peter ^a   Holmes, Andrew B ^b   Gaffney, Piers R J ^c   Reese, Colin B ^c   Cohen, Philip ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b Department of Chemistry   (United Kingdom)

^c KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0031127305     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/s0960-9822(06)00122-9     Document Type: Article

References (30)

1. Coffer PJ, Woodgett JR: Molecular-cloning and characterization of a novel putative protein-serine kinase related to the cAMP-dependent and protein-kinase-C families. Eur J Biochem 1991, 201:475-481.
2. Jones PF, Jakubowicz T, Pitossi FJ, Maurer F, Hemmings BA: Molecular-cloning and identification of a serine threonine protein-kinase of the 2nd-messenger subfamily. Proc Natl Acad Sci USA 1991, 88:4171-4175.
3. Belacossa A, Testa JR, Staal SP, Tsichlis PN: A retroviral oncogene, AKT, encoding a serine-threonine kinase containing an SH2-like region. Science 1991, 254:244-247.
4. Franke TF, Yang SI, Chan TO, Datta K, Kazlauskas A, Morrison DK, et al.: The protein-kinase encoded by the AKT protooncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 1995, 81:727-736.
5. Burgering BMT, Coffer PJ: Prate in-kinase-B (C-AKT) in phosphatidylinositol-3-OH kinase signal-transduction. Nature 1995, 376:599-602.
6. Kohn AD, Kovacina KS, Roth RA: Insulin stimulates the kinase-activity of RAC-PK, a pleckstrin homology domain-containing ser/thr kinase. EMBO J 1995, 14:4288-4295.
7. Cheng JQ, Godwin AK, Bellacosa A, Taguchi T, Franke TF, Hamilton TC, et al.: AKT2, a putative oncogene encoding a member of a subfamily of protein-serine threonine kinases, is amplified in human ovarian carcinomas. Proc Natl Acad Sci USA 1992, 89:9267-9271.
8. Cheng JQ, Ruggeri B, Klein WM, Sonoda G, Altomare DA, Watson DK, Testa JR: Amplification of AKT2 in human pancreatic-cancer cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA. Proc Natl Acad Sci USA 1996, 933636-3641.
9. Cross DAE, Alessi DR, Cohen P, Andjelkovic M, Hemmings BA: Inhibition of glycogen-synthase kinase-3 by insulin-mediated by protein-kinase-B. Nature 1995, 378:785-789.
10. Welsh GI, Foulstone EJ, Young SJ, Tavare JM, Proud CG: Wortmannin inhibits the effects of insulin and serum on the activities of glycogen-synthase kinase-3 and the mitogen-activated protein-kinase. Biochem J 1994, 303:15-20.
11. Kohn AD, Summers SA, Birnbaum MJ, Roth RA: Expression of a constitutively active AKT ser/thr kinase in 3T3-L1 adipocytes stimulates glucose-uptake and glucose-transporter-4 translocation. J Biol Chem 1996, 271:31372-31378.
12. Dudek H, Datta SR, Franke TF, Birnbarun MJ, Yao R, Cooper GM, et al.: Regulation of neuronal survival by the serine-threonine protein kinase AKT. Science 1997, 275:661-665.
13. Kauffmann-Zeh A, Rodriguez-Viciana P, Ulrich E, Gilbert, C, Coffer P, Downward J, Evan G: Suppression of c-Myc-induced apoptosis by Ras signalling through PI(3)K and PKB. Nature 1997, 385:544-548.
14. Andjelkovic M, Jakubowicz T, Cron P, Ming XF, Han JH, Hemmings BA: Activation and phosphorylation of a pleckstrin homology domain-containing protein-kinase (RAC-PK/PKB) promoted by serum and protein phosphatase inhibitors. Proc Natl Acad Sci USA 1996, 93:5699-5704.
15. Alessi DR, Andjelkovic M, Caudwell FB, Cron P, Morrice N, Cohen P, Hemmings B: Mechanism of activation of protein kinase B by insulin and IGF-1. EMBO J 1996, 15:6541-6551.
16. Alessi DR, Caudwell FB, Andjelkovic M, Hemmings BA, Cohen P: Molecular basis for the substrate specificity of protein kinase B; comparison with MAPKAP kinase-1 and p70 S6 kinase. FEBS Lett 1996, 399:333-338.
17. Baker RR, Thompson W: Selective acylation of 1-acylglycerophosphorylinositol by rat brain microsomes. Comparison with 1-acylglycerophosphorylcholine. J Biol Chem 1973, 248:7060-7065.
18. James SR, Downes CP, Gigg R, Grove SJA, Holmes AB, Alessi DR: Specific binding of the AKT-1 protein-kinase to phosphatidylinositol 3,4,5-trisphosphate without subsequent activation. Biochem J 1996, 315:709-713.
19. Frech M, Andjelkovic M, Falck JR, Hemmings BA: High affinity binding of inositol phosphates and phosphoinositides to the pleckstrin homology domain of RAC/protein kinase B and their influence on the kinase activity. J Biol Chem 1996, 272:8474-8480.
20. Klippel A, Kavanaugh WM, Pot D, Williams LT: A specific product of phosphatidyinositol 3-kinase directly activates the protein kinase Akt through its pleckstrin homology domain. Mol Cell Biol 1997, 17:338-344.
21. Franke TF, Kaplan DR, Cantley LC, Toker A: Direct regulation of the Akt proto-oncogene product by phosphatidylinositol-3,4-bisphosphate. Science 275:665-668.
22. Krebs EG, Love DS, Bratvold GE, Trayser KA, Meyer WL, Fischer EH: Purification and properties of rabbit skeletal muscle phosphorylase B kinase. Biochemistry 1964, 3:1023-1033.
23. Walsh DA, Perkins JP, Krebs EG : An adenosine 3′5′ monophosphate-dependent protein kinase from rabbit skeletal muscle. J Biol Chem 1968, 246:1968-1976.
24. Kohn AD, Takeuchi F, Roth RA: AKT, a pleckstrin homology domain-containing kinase, is activated primarily by phosphorylation. J Biol Chem 1996, 271:21920-21926.
25. James SR, Demel RA, Downes CP: Interfacial hydrolysis of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate by turkey erythrocyte phospholipase-C. Biochem J 1994, 298:499-506.
26. Sanchez I, Hughes RT, Mayer BJ, Yee K, Woodgett JR, Avruch J, et al.: Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun. Nature 1994, 372:794-798.
27. Alessi DR, Cohen P, Leevers S, Cowley S, Marshall CJ: Assay and expression of mitogen-activated protein kinase, MAP kinase kinase and Raf. Methods Enzymol 1995, 255:279-290.
28. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 1976, 72:248-254.
29. Stokoe D, Campbell DG, Nakielny S, Hidaka H, Leevers SJ, Marshall C, Cohen P: Mapkap kinase-2 - a novel protein-kinase activated by the mitogen-activated protein-kinase. EMBO J 1992, 11:3985-3994.
30. Vanhaesbroeck B, Leevers SJ, Panayotoy G, Waterfield MD: Phosphoinositide 3 kinases: a conserved family of signal transducers. Trends Biochem Sci 1997, in press.