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Volumn 281, Issue 5385, 1998, Pages 2042-2045

Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVATOR; PHOSPHATIDYLINOSITIDE; PROTEIN KINASE; PROTEIN KINASE C;

EID: 0032566691     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.281.5385.2042     Document Type: Article
Times cited : (983)

References (27)
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    • (1998) Science , vol.279 , pp. 707
    • Pullen, N.1
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    • 3543113199 scopus 로고    scopus 로고
    • note
    • Phosphorylation site-specific antisera were generated with oligopeptides seven residues long encompassing the phosphoamino acid at residue 4. The peptides were coupled to keyhole limpet hemocyanin and used to immunize rabbits. Sera were characterized for specificity with dephospho- and phospho- forms of the peptide antigen. Protein immunoblotting analysis was carried out in the presence of dephosphopeptide (1 μg/ml) to suppress immunodetection of the dephosphorylated proteins.
  • 25
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    • note
    • Lysis buffer: 1% (v/v) Triton X-100, 20 mM tris-HCl (pH 7.5), leupeptin (50 μg/ml), aprotinin (50 μg/ml), 1 mM dithiothreitol, and 0.1 mM phenylmethylsulfonyl fluoride.
  • 27
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    • note
    • We thank R. Marais (ICR, London) for providing the myc-pEFlink vector. We are grateful to the Deutsche Forschungsgemeinschaft (W.H.Z.), to the Medical Research Council (P.C. and D.R.A.), and to The Royal Society (P.C.) for support of this work.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.