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note
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13C NMR: δ 171.0, 157.8, 135.3, 133.4, 129.2, 129.0, 129.0, 128.7, 127.4, 127.3, 78.4, 63.9, 55.7, 41.4, 41.0.
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note
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0 were the fluorescence at 585 nm of the FRET-labeled RNA with and without ligand, respectively. All binding experiments were done in duplicate and averaged during analysis in Prism (GraphPad). Data were collected using a Molecular Devices FlexStation 96-well array spectrofluorometer.
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