Ribosomal RNA is the target for oxazolidinones, a novel class of translational inhibitors

RNA

Volumn 5, Issue 7, 1999, Pages 939-946

  Matassova, N B ^a,b   Rodnina, M V ^a   Endermann, R ^c   Kroll, H P ^c   Pleiss, U ^c   Wild, H ^c   Wintermeyer, W ^a  

^a UNIVERSITY OF WITTEN HERDECKE   (Germany)

^b ENERGY SYSTEMS INSTITUTE   (Russian Federation)

^c BAYER AG   (Germany)

Author keywords

Antibiotics; E site; Ribosomal RNA; Translational inhibition; Translocation

Indexed keywords

BACTERIAL RNA; OXAZOLIDINE DERIVATIVE;

ANTIBACTERIAL ACTIVITY; ARTICLE; CROSS LINKING; DRUG EFFECT; DRUG TARGETING; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; RNA BINDING; RNA TRANSLATION; TRANSLATION REGULATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; POSIBACTERIA;

EID: 0033032414     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838299990210     Document Type: Article

References (33)

1. Allmang C, Mougel M, Westhof E, Ehresmann B, Ehresmann C. 1994. Role of conserved nucleotides in building the 16S rRNA binding site of E. coli ribosomal protein S8. Nucleic Acids Res 22:3708-3714.
2. Barta A, Kuechler E, Steiner G. 1990. Photoaffinity labeling of the peptidyltransferase region. In: Hill WE, Dahlberg A, Garret RA, Moore PB, Schlessinger D, Warner JR, eds. The ribosome. Washington, DC: American Society of Microbiology, pp 358-365.
3. Bogdanov A, Chichkova N, Kopylov A, Mankin A, Skripkin E. 1988. Surface topography of ribosomal RNA. Methods Enzymol 164: 440-456.
4. Borowski C, Rodnina MV, Wintermeyer W. 1996. Truncated elongation factor G lacking the G domain promotes translocation of the 3′ end but not of the anticodon domain of peptidyl-tRNA. Proc Natl Acad Sci USA 93:4202-4206.
5. Brickner SJ, Hutchinson MR, Barbachyn MR, Manninen PR, Ulaniwicz DA, Garmon SA, Grega KC, Hendges SK, Toops DS, Ford CW, Zurenko GE. 1996. Synthesis and antimicrobial activity of U-100592 and U-100766, two oxazolidinone antibacterial agents for the potential treatment of multidrug-resistant gram-positive bacterial infections. J Med Chem 39:673-679.
6. Calogero RA, Pon CL, Canonaco MA, Gualerzi CO. 1988. Selection of the mRNA translation initiation region by Escherichia coli ribosomes. Proc Natl Acad Sci USA 85:6427-6431.
7. Carlson RK, Gregory WA, Park C. 1990. European Patent Application 352781.
8. Daly JS, Eliopoulos GM, Willey S, Moellering RC. 1988. Mechanism of action and in vitro and in vivo activities of S-6123, a new oxazolidinone compound. Antimicrob Agents Chemother 32:1341-1346.
9. Douthwaite S. 1992. Interaction of the antibiotics clindamycin and lincomycin with Escherichia coli 235 ribosomal RNA. Nucleic Acids Res 20:4717-4720.
10. Ehresmann C, Moine H, Mougel M, Dondon J, Grunberg-Manago M, Ebel JP, Ehresmann B. 1986. Cross-linking of initiation factor IF3 to Escherichia coli 30S ribosomal subunit by transdiamminedichloroplatinum(II): Characterization of two cross-linking sites in 16S rRNA; a possible way of functioning for IF3. Nucleic Acids Res 14:4803-4821.
11. Eustice DC, Feldman PA, Slee AM. 1988a. Mechanism of action of DuP 721, a new antibacterial agent: Effect on macromolecular synthesis. Biochim Biophys Res Commun 150:965-971.
12. Eustice DC, Feldman PA, Zajac I, Slee AM. 1988b. Mechanism of action of DuP 721: Inhibition of an early event during initiation of protein synthesis. Antimicrob Agents Chemother 32:1218-1222.
13. Ford CW, Hamel JC, Wilson DM, Moerman JK, Stapert D, Yancey RJ, Hutchinson DK, Barbachyn MR, Brickner SJ. 1996. In vivo activities of U-100592 and U-100766, novel oxazolidinone antimicrobial agents, against experimental bacterial infections. Antimicrob Agents Chemother 40:1508-1513.
14. Kalurachchi K, Uma K, Zimmermann RA, Nikonowicz EP. 1997. Structural features of the binding site for ribosomal protein S8 in Escherichia coli 16S rRNA defined using NMR spectroscopy. Proc Natl Acad Sci USA 94:2139-2144.
15. Kruse TA, Siboska GE, Clark BFC. 1982. Photosensitized crosslinking of tRNA to the P, A, and R sites of Escherichia coli ribosomes. Biochimie 64:279-284.
16. Lill R, Robertson JM, Wintermeyer W. 1989. Binding of the 3′ terminus of tRNA to 23S rRNA in the ribosomal exit site actively promotes translocation. EMBO J 8:3933-3938.
17. Lin AH, Murray RW, Vidmar TJ, Marotti KR. 1997. The oxazolidinone eperezolid binds to the 50S ribosomal subunit and competes with binding of chloramphenicol and linkomycin. Antimicrob Agents Chemother 41:2127-2131.
18. Moazed D, Noller HF. 1987. Chloramphenicol, erythromycin, carbomycin, and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNA. Biochimie 69:879-884.
19. Moazed D, Noller HF. 1989. Interaction of tRNA with 23S rRNA in the ribosomal A, P, and E sites. Cell 57:585-597.
20. Mougel M, Eyermann F, Westhof E, Romby P, Expert-Bezançon A, Ebel J-P, Ehresmann B, Ehresmann C. 1987. Binding of E. coli ribosomal protein S8 to 16S rRNA. A model for the interaction and the tertiary structure of the RNA binding site. J Mol Biol 198:91-107.
21. Mueller F, Brimacombe R. 1997a. A new model for the three-dimensional folding of Escherichia coli 16S ribosomal RNA. I. Fitting the RNA to a 3D electron microscopic map at 20 Å. J Mol Biol 271:524-544.
22. Mueller F, Brimacombe R. 1997b. A new model for the three-dimensional folding of Escherichia coli 16S ribosomal RNA. II. The RNA-protein interaction data. J Mol Biol 271:545-565.
23. Powers T, Noller HF. 1995. Hydroxyl radical footprinting of ribosomal proteins on 16S rRNA. RNA 1:194-209.
24. Rodnina MV, Fricke R, Wintermeyer W. 1994a. Transient conformational states of aminoacyl-tRNA during ribosome binding catalyzed by elongation factor Tu. Biochemistry 33:12267-12275.
25. fMet by Fast Protein Liquid Chromatography. Anal Biochem 219:380-381.
26. Rodnina MV, Wintermeyer W. 1995. GTP consumption of elongation factor Tu during translation of heteropolymeric mRNAs. Proc Natl Acad Sci USA 92:1945-1949.
27. Said B, Cole JR, Nomura M. 1988. Mutational analysis of the L1 binding site of 23S rRNA in Escherichia coli. Nucleic Acids Res 16:10529-10545.
28. Shinabarger DL, Marotti KR, Murray RW, Lin AH, Melchior EP, Swaney SM, Dunyak DS, Demyan WF, Buysse JM. 1997. Mechanism of action of oxazolidinones: Effect of linezolid and eperezolid on translation reactions. Antimicrob Agents Chemother 41:2132-2136.
29. Slee AM, Wuanola MA, McRipley RJ, Zajac I, Bartolomew PT, Gregory WA, Forbes M. 1987. Oxazolidinones, a new class of synthetic antibacterial agents: In vitro and in vivo activities of DuP 105 and Dup 721. Antimicrob Agents Chemother 31:1791-1797.
30. Stade K, Rinke-Appel J, Brimacombe R. 1989. Site-directed cross-linking of mRNA analogues to the Escherichia coli ribosome; identification of 30S ribosomal components that can be cross-linked to the mRNA at various points 5' with respect to the decoding site. Nucleic Acids Res 17:9889-9908.
31. Stern S, Moazed D, Noller HF. 1988. Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension. Methods Enzymol 164:481-489.
32. Wintermeyer W, Lill R, Robertson JM. 1990. Role of the tRNA exit site in ribosomal translocation. In: Hill WE, Dahlberg A, Garret RA, Moore PB, Schlessinger D, Warner JR, eds. The ribosome. Washington, DC: American Society of Microbiology, pp 348-357.
33. Wollenzien P. 1988. Isolation and identification of RNA cross-links. Methods Enzymol 164:319-329.