Splicing factor hSlu7 contains a unique functional domain required to retain the protein within the nucleus

Molecular Biology of the Cell

Volumn 15, Issue 8, 2004, Pages 3782-3795

  Shomron, Noam ^a   Reznik, Mika ^a   Ast, Gil ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

EXPORTIN 1; HSLU7 PROTEIN; MESSENGER RNA PRECURSOR; SMALL NUCLEAR RIBONUCLEOPROTEIN; UNCLASSIFIED DRUG;

ARTICLE; ATOMIC EMISSION SPECTROMETRY; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE MICROSCOPY; NUCLEAR LOCALIZATION SIGNAL; NUCLEOCYTOPLASMIC TRANSPORT; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; SITE DIRECTED MUTAGENESIS; SPLICEOSOME; WESTERN BLOTTING; ZINC FINGER MOTIF;

AMINO ACID SEQUENCE; CELL LINE; CELL NUCLEUS; COMPUTATIONAL BIOLOGY; HUMANS; KARYOPHERINS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR LOCALIZATION SIGNALS; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RIBONUCLEOPROTEINS, SMALL NUCLEAR; RNA SPLICING; SEQUENCE HOMOLOGY, AMINO ACID; ZINC FINGERS;

EID: 3343013149     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E04-02-0152     Document Type: Article

References (70)

1. Ast, G., and Weiner, A.M. (1986). A U1/U4/U5 snRNP complex induced by a 2′-O-methyl-oligoribonucleotide complementary to U5 snRNA. Science. 272, 881-884.
2. Banerjee, H., Rahn, A., Gawande, B., Guth, S., Valcarcel, J., and Singh, R. (2004). The conserved RNA recognition motif 3 of U2 snRNA auxiliary factor (U2AF 65) in essential in vivo but dispensable for activity in vitro. RNA 10, 240-253.
3. Boulikas, T. (1993). Nuclear localization signals (NLS). Crit. Rev. Eukaryot. Gene Expr. 3, 193-227.
4. Braem, C.V., Kas, K., Meyen, E., Debiec-Rychter, M., Van De Ven, W.J., and Voz, M.L. (2002). Identification of a karyopherin alpha 2 recognition site in PLAG1, which functions as a nuclear localization signal. J. Biol. Chem. 277, 19673-19678.
5. Brow, D.A. (2002). Allosteric cascade of spliceosome activation. Annu. Rev. Genet. 36, 333-360.
6. Brown, V., Krynetski, E., Krynetskaia, N., Grieger, D., Mukatira, S., Murti, K., Slaughter, C., Park, H.W., and Evans, W. (2003). A novel CRM1-mediated nuclear export signal governs nuclear accumulation of GAPDH following genotoxic stress. J. Biol. Chem. 279, 5984-5992.
7. Burdette, S.C., and Lippard, S.J. (2003). Meeting of the minds: metalloneurochemistry. Proc. Natl. Acad. Sci. USA 100, 3605-3610.
8. Burge, C.B., Tuschl, T., and Sharp, P.A. (1999). Splicing of precursors to mRNA by the spliceosome. In: The RNA World, ed. R.F. Gesteland and J.F. Atkins, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 525-560.
9. Caceres, J.F., Misteli, T., Screaton, G.R., Spector, D.L., and Krainer, A.R. (1997). Role of the modular domains of SR proteins in subnuclear localization and alternative splicing specificity. J. Cell Biol. 138, 225-238.
10. Chakraburtty, K. (1999). Functional interaction of yeast elongation factor 3 with yeast ribosomes. Int. J. Biochem. Cell Biol. 31, 163-173.
11. Changela, A., Chen, K., Xue, Y., Holschen, J., Outten, C.E., O'Halloran, T.V., and Mondragon, A. (2003). Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR. Science 301, 1383-1387.
12. Chua, K., and Reed, R. (1999a). Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing. Genes Dev. 13, 841-850.
13. Chua, K., and Reed, R. (1999b). The RNA splicing factor hSlu7 is required for correct 3′ splice-site choice. Nature 402, 207-210.
14. Cokol, M., Nair, R., and Rost, B. (2000). Finding nuclear localization signals. EMBO Rep. 1, 411-415.
15. Das, S., and Maitra, U. (2001). Functional significance and mechanism of eIF5-promoted GTP hydrolysis in eukaryotic translation initiation. Prog. Nucleic Acid Res. Mol. Biol. 70, 207-231.
16. Dye, B.T., and Patton, J.G. (2001). An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles. Exp. Cell Res. 263, 131-144.
17. Fahrenkrog, B., and Aebi, U. (2003). The nuclear pore complex: nucleocytoplasmic transport and beyond. Nat. Rev. Mol. Cell. Biol. 4, 757-766.
18. Frank, D., and Guthrie, C. (1992). An essential splicing factor, SLU7, mediates 3′ splice site choice in yeast. Genes Dev. 6, 2112-2124.
19. Frank, D., Patterson, B., and Guthrie, C. (1992). Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing. Mol. Cell. Biol. 12, 5197-5205.
20. Frugier, M., Moulinier, L., and Giege, R. (2000). A domain in the N-terminal extension of class IIb eukaryotic aminoacyl-tRNA synthetases is important for tRNA binding. EMBO J. 19, 2371-2380.
21. Fukuda, M., Asano, S., Nakamura, T., Adachi, M., Yoshida, M., Yanagida, M., and Nishida, E. (1997). CRM1 is responsible for intracellular transport mediated by the nuclear export signal. Nature 390, 308-311.
22. Ha, S., Park, S., Yun, C.H., and Choi, Y. (2002). Characterization of nuclear localization signal in mouse ING1 homolog protein. Biochem. Biophys. Res. Commun. 293, 163-166.
23. Hanamura, A., Caceres, J.F., Mayeda, A., Franza, B.R., Jr., and Krainer, A.R. (1998). Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. RNA 4, 430-444.
24. Harris, E.D. (2002). Cellular transporters for zinc. Nutr. Rev. 60, 121-124.
25. Hastings, M.L., and Krainer, A.R. (2001). Pre-mRNA splicing in the new millennium. Curr. Opin. Cell Biol. 13, 302-309.
26. Henderson, B.R., and Eleftheriou, A. (2000). A comparison of the activity, sequence specificity, and CRM1-dependence of different nuclear export signals. Exp. Cell Res. 256, 213-224.
27. James, S.A., Turner, W., and Schwer, B. (2002). How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA 8, 1068-1077.
28. Jensen, K.B., Dredge, B.K., Stefani, G., Zhong, R., Buckanovich, R.J., Okano, H.J., Yang, Y.Y., and Darnell, R.B. (2000). Nova-1 regulates neuron-specific alternative splicing and is essential for neuronal viability. Neuron 25, 359-371.
29. Jiang, J., Horowitz, D.S., and Xu, R.M. (2000). Crystal structure of the functional domain of the splicing factor Prp18. Proc. Natl. Acad. Sci. USA 97, 3022-3027.
30. Jones, M.H., Frank, D.N., and Guthrie, C. (1995). Characterization and functional ordering of Slu7p and Prp17p during the second step of pre-mRNA splicing in yeast. Proc. Natl. Acad. Sci. USA 92, 9687-9691.
31. Jurica, M.S., and Moore, M.J. (2003). Pre-mRNA splicing: awash in a sea of proteins. Mol. Cell 12, 5-14.
32. Kitagawa, H., Hotta, Y., Fujiki, K., and Kanai, A. (1996). Cloning and high expression of rabbit FKBP25 in cornea. Jpn. J. Ophthalmol. 40, 133-141.
33. Komeili, A., and O'Shea, E.K. (2000). Nuclear transport and transcription. Curr. Opin. Cell Biol. 12, 355-360.
34. Konarska, M.M., and Sharp, P.A. (1986). Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs. Cell 46, 845-855.
35. Kudo, N., Matsumori, N., Taoka, H., Fujiwara, D., Schreiner, E.P., Wolff, B., Yoshida, M., and Horinouchi, S. (1999). Leptomycin B inactivates CRM1/ exportin 1 by covalent modification at a cysteine residue in the central conserved region. Proc. Natl. Acad. Sci. USA 96, 9112-9117.
36. Lallena, M.J., Chalmers, K.J., Llamazares, S., Lamond, A.L, and Valcarcel, J. (2002). Splicing regulation at the second catalytic step by Sex-lethal involves 3′ splice site recognition by SPF45. Cell 109, 285-296.
37. Lamond, A.I., and Spector, D.L. (2003). Nuclear speckles: a model for nuclear organelles. Nat. Rev. Mol. Cell. Biol. 4, 605-612.
38. Leary, D.J., Terns, M.P., and Huang, S. (2003). Components of U3 snoRNA containing complexes shuttle between nuclei and the cytoplasm and differentially localize in nucleoli: implications for assembly and function. Mol. Biol. Cell 15, 281-293.
39. Lev-Maor, G., Sorek, R., Shomron, N., and Ast, G. (2003). The birth of an alternatively spliced exon: 3′ splice-site selection in Alu exons. Science 300, 1288-1291.
40. Liu, X., Jiang, Q., Mansfield, S.G., Puttaraju, M., Zhang, Y., Zhou, W., Cohn, J.A., Garcia-Blanco, M.A., Mitchell, L.G., and Engelhardt, J.F. (2002). Partial correction of endogenous DeltaF508 CFTR in human cystic fibrosis airway epithelia by spliceosome-mediated RNA trans-splicing. Nat. Biotechnol. 20, 47-52.
41. Lopato, S., Gattoni, R., Fabini, G., Stevenin, J., and Barta, A. (1999). A novel family of plant splicing factors with a Zn knuckle motif: examination of RNA binding and splicing activities. Plant Mol. Biol. 39, 761-773.
42. Mahe, D., Fischer, N., Decimo, D., and Fuchs, J.P. (2000). Spatiotemporal regulation of hnRNP M and 2H9 gene expression during mouse embryonic development. Biochim. Biophys. Acta 1492, 414-424.
43. Meshorer, E., Erb, C., Gazit, R., Pavlovsky, L., Kaufer, D., Friedman, A., Click, D., Ben-Arie, N., and Soreq, H. (2002). Alternative splicing and neuritic mRNA translocation under long-term neuronal hypersensitivity. Science 295, 508-512.
44. Mintz, P.J., and Spector, D.L. (2000). Compartmentalization of RNA processing factors within nuclear speckles. J. Struct. Biol. 229, 241-251.
45. Modrek, B., and Lee, C. (2002). A genomic view of alternative splicing. Nat. Genet. 30, 13-19.
46. O'Halloran, T.V. (1993). Transition metals in control of gene expression. Science 261, 715-725.
47. O'Halloran, T.V., and Culotta, V.C. (2000). Metallochaperones, an intracellular shuttle service for metal ions. J. Biol. Chem. 275, 25057-25060.
48. Ossareh-Nazari, B., Bachelerie, F., and Dargemont, C. (1997). Evidence for a role of CRM1 in signal-mediated nuclear protein export. Science 278, 141-144.
49. Pandya, K., and Townes, T.M. (2002). Basic residues within the Kruppel zinc finger DNA binding domains are the critical nuclear localization determinants of EKLF/KLF-1. J. Biol. Chem. 277, 16304-16312.
50. Parent, L.J., Wilson, C.B., Resh, M.D., and Wills, J.W. (1996). Evidence for a second function of the MA sequence in the Rous sarcoma virus Gag protein. J. Virol. 70, 1016-1026.
51. Rappsilber, J., Ryder, U., Lamond, A.I., and Mann, M. (2002). Large-scale proteomic analysis of the human spliceosome. Genome Res. 12, 1231-1245.
52. Robbins, J., Dilworth, S.M., Laskey, R.A., and Dingwall, C. (1991). Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell 64, 615-623.
53. Romanelli, M.G., and Morandi, C. (2002). Importin alpha binds to an unusual bipartite nuclear localization signal in the heterogeneous ribonucleoprotein type I. Eur. J. Biochem. 269, 2727-2734.
54. Rosenzweig, A.C. (2002). Metallochaperones: bind and deliver. Chem. Biol. 9, 673-677.
55. Scarlata, S., and Carter, C. (2003). Role of HIV-1 Gag domains in viral assembly. Biochim. Biophys. Acta 2614, 62-72.
56. Shomron, N., and Ast, G. (2003). Boric acid reversibly inhibits the second step of pre-mRNA splicing. FEBS Lett. 552, 219-224.
57. Shomron, N., Malca, H., Vig, I., and Ast, G. (2002). Reversible inhibition of the second step of splicing suggests a possible role of zinc in the second step of splicing. Nucleic Acids Res. 30, 4127-4137.
58. Smith, C.W., and Valcarcel, J. (2000). Alternative pre-mRNA splicing: the logic of combinatorial control. Trends Biochem. Sci. 25, 381-388.
59. Stoilov, P., Meshorer, E., Gencheva, M., Click, D., Soreq, H., and Stamm, S. (2002). Defects in pre-mRNA processing as causes of and predisposition to diseases. DNA Cell Biol. 21, 803-818.
60. Stros, M. (2001). Two mutations of basic residues within the N-terminus of HMG-1 B domain with different effects on DNA supercoiling and binding to bent DNA. Biochemistry 40, 4769-4779.
61. Umen, J.G., and Guthrie, C. (1995). The second catalytic step of pre-mRNA splicing. RNA 1, 869-885.
62. van der Houven van Oordt, W., Diaz-Meco, M.T., Lozano, J., Krainer, A.R., Moscat, J., and Caceres, J.F. (2000). The MKK(3/6)-p38-signaling cascade alters the subcellular distribution of hnRNP A1 and modulates alternative splicing regulation. J. Cell Biol. 149, 307-316.
63. Wang, J., and Manley, J.L. (1995). Overexpression of the SR proteins ASF/SF2 and SC35 influences alternative splicing in vivo in diverse ways. RNA 1, 335-346.
64. Watjen, W., Haase, H., Biagioli, M., and Beyersmann, D. (2002). Induction of apoptosis in mammalian cells by cadmium and zinc. Environ Health Perspect. 330, 865-867.
65. Weeks, K.M., and Crothers, D.M. (1992). RNA binding assays for Tat-derived peptides: implications for specificity. Biochemistry 31, 10281-10287.
66. Xiao, Z., Latek, R., and Lodish, H.F. (2003). An extended bipartite nuclear localization signal in Smad4 is required for its nuclear import and transcriptional activity. Oncogene 22, 1057-1069.
67. Xie, J., Lee, J.A., Kress, T.L., Mowry, K.L., and Black, D.L. (2003). Protein kinase A phosphorylation modulates transport of the polypyrimidine tract-binding protein. Proc. Natl. Acad. Sci. USA 300, 8776-8781.
68. Yu, Z., Lee, C.H., Chinpaisal, C., and Wei, L.N. (1998). A constitutive nuclear localization signal from the second zinc-finger of orphan nuclear receptor TR2. J. Endocrinol. 359, 53-60.
69. Zhang, X., and Schwer, B. (1997). Functional and physical interaction between the yeast splicing factors Slu7 and Prp18. Nucleic Acids Res. 25, 2146-2152.
70. Zhou, W., Parent, L.J., Wills, J.W., and Resh, M.D. (1994). Identification of a membrane-binding domain within the amino-terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholipids. J. Virol. 68, 2556-2569.