An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles

Experimental Cell Research

Volumn 263, Issue 1, 2001, Pages 131-144

  Dye, B T ^a   Patton, J G ^a  

^a VANDERBILT UNIVERSITY   (United States)

Author keywords

FHL2; Nuclear localization; PSF; Speckles; Splicing; Subnuclear localization

Indexed keywords

BINDING PROTEIN; GLUTAMINE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LIM PROTEIN; PROLINE; PROTEIN FHL2; PROTEIN PSF; RNA; RNA BINDING PROTEIN; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL NUCLEUS; CONTROLLED STUDY; GENE DELETION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; MOLECULAR RECOGNITION; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; RNA SPLICING; TWO HYBRID SYSTEM; YEAST;

EID: 0035242433     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.2000.5097     Document Type: Article

References (91)

1. Regulation of pre-mRNA splicing in metazoa
2. Biochemistry and regulation of pre-mRNA splicing
3. The structure and function of proteins involved in mammalian pre-mRNA splicing
4. Alternative pre-mRNA splicing: The logic of combinatorial control
5. Alternative pre-mRNA splicing
6. Mechanisms of regulated pre-mRNA splicing
7. snRNP proteins
8. Determinants of SR protein specificity
9. SR proteins: A conserved family of pre-mRNA splicing factors
10. General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer
11. A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA
12. A splicing enhancer exhibits both constitutive and regulated activities
13. Identification of a new class of exonic splicing enhancers by in vivo selection
14. Enhancer elements activate the weak 3′ splice site of α-tropomyosin exon 2
15. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
16. Protein-protein interactions and 5′ splice site recognition in mammalian mRNA precursors
17. SR proteins promote the first specific recognition of pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex
18. The cellular organization of gene expression
19. Nuclear organization of pre-mRNA splicing factors
20. Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery
21. Dynamic interactions between splicing snRNPs, coiled bodies, and nucleoli revealed using snRNP fusions to the green fluorescent protein
22. Nuclear bodies: Multifaceted subdomains of the interchromatin space
23. Newly assembled snRNPs associate with coiled bodies before speckles, suggesting a nuclear snRNP maturation pathway
24. The localization of sites containing nascent RNA and splicing factors
25. Ultrastructure of the nucleus in relation to transcription and splicing: Roles of perichromatin fibrils and interchromatin granules
26. Quantitative digital analysis of diffuse and concentrated nuclear distributions of nascent transcripts, SC35 and poly(A)
27. Ultrastructural analysis of transcription and splicing in the cell nucleus after bromo-UTP microinjection
28. Splicing factors associate with HCMV-IE transcripts after transcriptional activation of the gene, but dissociate upon transcription inhibition: Evidence for a dynamic organization of splicing factors
29. The dynamics of a pre-mRNA splicing factor in living cells
30. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription
31. Dynamic relocation of transcription and splicing factors dependent upon transcriptional activity
32. Discrete nuclear domains of poly(A) RNA and their relationship to functional organization of the nucleus
33. Localization of pre-messenger RNA at discrete nuclear sites
34. A three-dimensional view of precursor messenger RNA metabolism within the mammalian nucleus
35. Intranuclear distribution of poly(A) RNA determined by electron microscope in situ hybridization
36. Higher level organization of individual gene transcription and RNA splicing
37. Dynamic organization of pre-mRNA splicing factors
38. Three-dimensional visualization of transcription sites and their association with splicing factor-rich nuclear speckles
39. Processing of endogenous pre-mRNAs in association with SC-35 domains is gene specific
40. Nonrandom gene organization: Structural arrangements of specific pre-mRNA transcription and splicing with SC-35 domains
41. Intron-independent association of splicing factors with active genes
42. Nuclear pre-mRNA compartmentalization: Trafficking of released transcripts to splicing factor reservoirs
43. Arginine/serine-rich domains of the su(wa) and tra RNA processing regulators target proteins to a subnuclear compartment implicated in splicing
44. An amino acid sequence motif sufficient for subnuclear localization of an arginine/serine-rich splicing factor
45. Role of the modular domains of SR proteins in subnuclear localization and alternative splicing specificity
46. The Sm core domain mediates targeting of U1 snRNP to subnuclear compartments involved in transcription and splicing
47. A novel set of spliceosome-associated proteins (SAPs) and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction
48. Cloning and characterization of PSF, a novel pre-mRNA splicing factor
49. Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B″ and their cognate RNAs
50. Multiple RRM domains contribute to RNA binding specificity for polypyrimidine tract binding protein
51. The U1 small ribonucleoprotein (snRNP) 70K protein is transported independently of U1 snRNP particles via a nuclear localization signal in the RNA-binding domain
52. Nuclear localization signals overlap DNA- or RNA-binding domains in nucleic acid-binding proteins
53. A simple and rapid method for generating a deletion by PCR
54. Cloning and domain structure of the mammalian splicing factor U2AF
55. 65 to sites of active splicing in the nucleus
56. Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB
57. The green fluorescent protein
58. Nuclear targeting sequences-a consensus?
59. Three basic regions in adenovirus DNA polymerase interact differentially depending on the protein context to function as bipartite nuclear localization signals
60. Transcription-dependent colocalization of the U1, U2, U4/U6, and U5 snRNPs in coiled bodies
61. Quantitative imaging of pre-mRNA splicing factors in living cells
62. Molecular cloning and characterization of FHL2, a novel LIM domain protein preferentially expressed in human heart
63. Isolation of genes differentially expressed in human primary myoblasts and embryonal rhabdomyosarcoma
64. LIM domains: Multiple roles as adapters and functional modifiers in protein interactions
65. Transport of macromolecules between the nucleus and cytoplasm
66. Transport of proteins and RNAs in and out of the nucleus
67. Transport pathways of macromolecules between the nucleus and the cytoplasm
68. Functional modules in ribosomal protein L5 for ribonucleoprotein complex formation and nucleocytoplasmic transport
69. Comparative mutagenesis of nuclear localization signals reveal the importance of neutral and acidic amino acids
70. nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6
71. The human U5 snRNP-specific 100kD protein is an RS domain-containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p
72. Protein-protein interaction of FHL2, a LIM domain protein preferentially expressed in human heart, with hCDC47
73. FHL2, a novel, tissue-specific coactivator of the androgen receptor
74. Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA
75. Human U1 snRNP-specific C protein: Complete cDNA and protein sequence and identification of a multigene family in mammals
76. Splicing factor SF3a60 is the mammalian homologue of PRP9 of S. cerevisiae: The conserved zinc finger-like motif is functionally exchangeable in vivo
77. Correspondence between a mammalian spliceosome component and an essential yeast splicing factor
78. Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae
79. Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing
80. RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus
81. The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors
82. An essential splicing factor, Slu7, mediates 3′ splice site choice in yeast
83. The Saccharomyces cerevisiae PRP21 gene product is an integral component of the prespliceosome
84. Direct interaction of the KRAB zinc finger protein ZNF74 with a hyperphosphorylated form of the RNA polymerase II largest subunit
85. Characterization and cloning of the human splicing factor 9G8: A novel 35 kDa factor of the serine/arginine protein family
86. Zis: A developmentally regulated gene expressed in juxtaglomerular cells
87. Identification, characterization and mapping of the human ZIS (zinc-finger, splicing) gene
88. A novel family of plant splicing factors with a Zn knuckle motif: Examination of RNA binding and splicing activities
89. Homodimerization of the human U1-specific protein C
90. The herpes simplex virus type 1 regulatory protein ICP27 coimmunoprecipitates with anti-Sm antiserum, and the C terminus appears to be required for this interaction