The birth of an alternatively spliced exon: 3′ Splice-site selection in Alu exons

Science

Volumn 300, Issue 5623, 2003, Pages 1288-1291

  Lev Maor, Galit ^a   Sorek, Rotem ^a,b   Shomron, Noam ^a   Ast, Gil ^a  

^a TEL AVIV UNIVERSITY   (Israel)

^b Compugen ^*  (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

GENETIC ENGINEERING; MUTAGENESIS; RNA;

EXONIZATION;

MOLECULAR BIOLOGY;

MESSENGER RNA;

BIOCHEMISTRY; GENETICS;

ALTERNATIVE RNA SPLICING; ALU SEQUENCE; ARTICLE; DNA RESPONSIVE ELEMENT; EXON; GENE CONTROL; GENE INSERTION; GENE MUTATION; GENE SEQUENCE; HUMAN; INTERSPERSED REPEAT; INTRON; PRIORITY JOURNAL; PROTEIN MOTIF; SEQUENCE ANALYSIS; SPLICEOSOME;

ADENOSINE DEAMINASE; ALTERNATIVE SPLICING; ALU ELEMENTS; CELL LINE; CLONING, MOLECULAR; DINUCLEOSIDE PHOSPHATES; DNA, ANTISENSE; EXONS; GENOME, HUMAN; GLUCOSYLTRANSFERASES; HUMANS; INTRONS; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; POLYMERASE CHAIN REACTION; RIBONUCLEOPROTEINS, SMALL NUCLEAR; SPLICEOSOMES; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0038701605     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1082588     Document Type: Article

References (29)

1. A. J. Mighell, A. F. Markham, P. A. Robinson, FEBS Lett. 417, 1 (1997).
2. D. J. Rowold, R. J. Herrera, Genetica 108, 57 (2000).
3. C. W. Schmid, Prog. Nucleic Acid Res. Mol. Biol. 53, 283 (1996).
4. E. S. Lander et al., Nature 409, 860 (2001).
5. A. M. Roy-Engel et al., Genome Res. 12, 1333 (2002).
6. W. Makalowski, G. A. Mitchell, D. Labuda, Trends Genet. 10, 188 (1994).
7. W. Makalowski, Gene 259, 61 (2000).
8. A. Nekrutenko, W. H. Li, Trends Genet. 17, 619 (2001).
9. R. Sorek, G. Ast, D. Graur, Genome Res. 12, 1060 (2002).
10. B. Knebelmann et al., Hum. Mol. Genet. 4, 675 (1995).
11. G. A. Mitchell et al., Proc. Natl. Acad. Sci. U.S.A. 88, 815 (1991).
12. R. Vervoort, R. Gitzelmann, W. Lissens, I. Liebaers, Hum. Genet. 103, 686 (1998).
13. K. Chua, R. Reed, Nature 402, 207 (1999).
14. K. Chua, R. Reed, Mol. Cell. Biol. 21, 1509 (2001).
15. R. E. Manrow, S. L. Berger, J. Mol. Biol. 234, 281 (1993).
16. D. Slavov, K. Gardiner, Gene 299, 83 (2002).
17. F. Lai, C. X. Chen, K. C. Carter, K. Nishikura, Mol. Cell. Biol. 17, 2413 (1997).
18. Materials and methods are available as supporting material on Science Online.
19. G. Lev-Maor, R. Sorek, N. Shomron, G. Ast, unpublished data.
20. R. Sorek, R. Shalgi, G. Ast, D. Graur, unpublished data.
21. J. Brosius, S. J. Gould, Proc. Natl. Acad. Sci. U.S.A. 89, 10706 (1992).
22. M. Mihovilovic et al., Biochem. Biophys. Res. Commun. 197, 137 (1993).
23. M. Miller, K. Zeller, Gene 190, 309 (1997).
24. W. G. Cance, R. J. Craven, T. M. Weiner, E. T. Liu, Int. J. Cancer 54, 571 (1993).
25. I. W. Caras et al, Nature 325, 545 (1987).
26. G. Svineng, R. Fassler, S. Johansson, Biochem. J. 330, 1255 (1998).
27. J. Jurka, A. Milosavljevic, J. Mol. Evol. 32, 105 (1991).
28. RepeatMasker is available online at http://repeatmasker.genome.washington.edu/cgi-bin/RepeatMasker.
29. We thank M. Kupiec for a critical reading; R. Reed for the hSlu7 plasmid; and also F. Belinky, R. Shalgi, T. Dagan, and E. Sharon for assistance in Alu data analysis. Supported by a grant from the Israel Science Foundation and, in part, by a grant from the Israel Cancer Association and the Indian-Israeli Scientific Research Corporation to G.A.