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Volumn 278, Issue 5335, 1997, Pages 141-144

Evidence for a role of CRM1 in signal-mediated nuclear protein export

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOME PROTEIN;

EID: 0030748907     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5335.141     Document Type: Article
Times cited : (632)

References (44)
  • 1
    • 0027937653 scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1994) Cell , vol.79 , pp. 767
    • Görlich, D.1    Prehn, S.2    Laskey, R.3    Hartmann, E.4
  • 2
    • 0028962511 scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1995) Proc. Natl. Acad. Sci. U.S.A. , vol.92 , pp. 2008
    • Moroianu, J.1    Blobel, G.2    Radu, A.3
  • 3
    • 0029093824 scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1995) EMBO J. , vol.14 , pp. 3617
    • Imamoto, N.1
  • 4
    • 0028988731 scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1995) Science , vol.268 , pp. 1049
    • Weis, K.1    Mattaj, I.W.2    Lamond, A.3
  • 5
    • 0029278621 scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1995) Curr. Biol. , vol.5 , pp. 383
    • Görlich, D.1
  • 6
    • 0028834428 scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1995) Cell , vol.83 , pp. 683
    • Rexach, M.1    Blobel, G.2
  • 7
    • 0029558543 scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1995) J. Cell Biol. , vol.131 , pp. 1699
    • Iovine, M.K.1    Watkins, J.L.2    Wente, S.R.3
  • 8
    • 0030221192 scopus 로고    scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1996) Curr. Biol. , vol.6 , pp. 1025
    • Makkerh, J.P.S.1    Dingwall, C.2    Laskey, R.A.3
  • 9
    • 0030595342 scopus 로고    scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1996) Cell , vol.86 , pp. 985
    • Pollard, V.W.1
  • 10
    • 0029845118 scopus 로고    scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1996) Science , vol.274 , pp. 624
    • Aitchison, J.D.1    Blobel, G.2    Rout, M.P.3
  • 11
    • 0030722506 scopus 로고    scopus 로고
    • D. Görlich, S. Prehn, R. Laskey, E. Hartmann, Cell 79, 767 (1994); J. Moroianu, G. Blobel, A. Radu, Proc. Natl. Acad. Sci. U.S.A. 92, 2008 (1995); N. Imamoto et al., EMBO J. 14, 3617 (1995); K. Weis, I. W. Mattaj, A. Lamond, Science 268, 1049 (1995); D. Görlich et al., Curr. Biol. 5, 383 (1995); M. Rexach and G. Blobel, Cell 83, 683 (1995); M. K. Iovine, J. L. Watkins, S. R. Wente, J. Cell Biol. 131, 1699 (1995); J. P. S. Makkerh, C. Dingwall, R. A. Laskey, Curr. Biol. 6, 1025 (1996); V. W. Pollard et al., Cell 86, 985 (1996); J. D. Aitchison, G. Blobel, M. P. Rout, Science 274, 624 (1996); M. Rout, G. Blobel, J. D. Aitchison, Cell 89, 715 (1997).
    • (1997) Cell , vol.89 , pp. 715
    • Rout, M.1    Blobel, G.2    Aitchison, J.D.3
  • 12
    • 0027376308 scopus 로고
    • M. S. Moore and G. Blobel, Nature 365, 661 (1993); F. Melchior, B. Paschal, J. Evans, L. Gerace, J. Cell Biol. 123, 215 (1993); B. Paschal and L. Gerace, ibid. 129, 925 (1995); U. Nehrbass and G. Blobel, Science 272, 120 (1996).
    • (1993) Nature , vol.365 , pp. 661
    • Moore, M.S.1    Blobel, G.2
  • 13
    • 0027376308 scopus 로고
    • M. S. Moore and G. Blobel, Nature 365, 661 (1993); F. Melchior, B. Paschal, J. Evans, L. Gerace, J. Cell Biol. 123, 215 (1993); B. Paschal and L. Gerace, ibid. 129, 925 (1995); U. Nehrbass and G. Blobel, Science 272, 120 (1996).
    • (1993) J. Cell Biol. , vol.123 , pp. 215
    • Melchior, F.1    Paschal, B.2    Evans, J.3    Gerace, L.4
  • 14
    • 0029027836 scopus 로고
    • M. S. Moore and G. Blobel, Nature 365, 661 (1993); F. Melchior, B. Paschal, J. Evans, L. Gerace, J. Cell Biol. 123, 215 (1993); B. Paschal and L. Gerace, ibid. 129, 925 (1995); U. Nehrbass and G. Blobel, Science 272, 120 (1996).
    • (1995) J. Cell Biol. , vol.129 , pp. 925
    • Paschal, B.1    Gerace, L.2
  • 15
    • 0030570091 scopus 로고    scopus 로고
    • M. S. Moore and G. Blobel, Nature 365, 661 (1993); F. Melchior, B. Paschal, J. Evans, L. Gerace, J. Cell Biol. 123, 215 (1993); B. Paschal and L. Gerace, ibid. 129, 925 (1995); U. Nehrbass and G. Blobel, Science 272, 120 (1996).
    • (1996) Science , vol.272 , pp. 120
    • Nehrbass, U.1    Blobel, G.2
  • 16
    • 0024654957 scopus 로고
    • Y. Adachi and M. Yanagida, J. Cell Biol. 108, 1195 (1989); T. Toda et al., Mol. Cell. Biol. 12, 5474 (1992); M. Fornerod et al., EMBO J. 16, 807 (1997).
    • (1989) J. Cell Biol. , vol.108 , pp. 1195
    • Adachi, Y.1    Yanagida, M.2
  • 17
    • 0026466825 scopus 로고
    • Y. Adachi and M. Yanagida, J. Cell Biol. 108, 1195 (1989); T. Toda et al., Mol. Cell. Biol. 12, 5474 (1992); M. Fornerod et al., EMBO J. 16, 807 (1997).
    • (1992) Mol. Cell. Biol. , vol.12 , pp. 5474
    • Toda, T.1
  • 18
    • 0031053791 scopus 로고    scopus 로고
    • Y. Adachi and M. Yanagida, J. Cell Biol. 108, 1195 (1989); T. Toda et al., Mol. Cell. Biol. 12, 5474 (1992); M. Fornerod et al., EMBO J. 16, 807 (1997).
    • (1997) EMBO J. , vol.16 , pp. 807
    • Fornerod, M.1
  • 21
    • 0029130169 scopus 로고    scopus 로고
    • U. Fischer, J. Huber, W. C. Boelens, I. W. Mattaj, R. Luhrmann, ibid. 82, 475 (1995); W. Wen, J. L. Meinkoth, R. Y. Tsien, S. S. Taylor, ibid., p. 463; F. Arenzana-Seisdedos et al., J. Cell Sci. 110, 369 (1997); W. M. Michael, M. Choi, G. Dreyfuss, Cell 83, 415 (1995); W. M. Michael, P. S. Eder, G. Dreyfuss, EMBO J. 16, 3587 (1997).
    • (1995) Cell , vol.82 , pp. 475
    • Fischer, U.1    Huber, J.2    Boelens, W.C.3    Mattaj, I.W.4    Luhrmann, R.5
  • 22
    • 0029130169 scopus 로고    scopus 로고
    • U. Fischer, J. Huber, W. C. Boelens, I. W. Mattaj, R. Luhrmann, ibid. 82, 475 (1995); W. Wen, J. L. Meinkoth, R. Y. Tsien, S. S. Taylor, ibid., p. 463; F. Arenzana-Seisdedos et al., J. Cell Sci. 110, 369 (1997); W. M. Michael, M. Choi, G. Dreyfuss, Cell 83, 415 (1995); W. M. Michael, P. S. Eder, G. Dreyfuss, EMBO J. 16, 3587 (1997).
    • Cell , pp. 463
    • Wen, W.1    Meinkoth, J.L.2    Tsien, R.Y.3    Taylor, S.S.4
  • 23
    • 0031057644 scopus 로고    scopus 로고
    • U. Fischer, J. Huber, W. C. Boelens, I. W. Mattaj, R. Luhrmann, ibid. 82, 475 (1995); W. Wen, J. L. Meinkoth, R. Y. Tsien, S. S. Taylor, ibid., p. 463; F. Arenzana-Seisdedos et al., J. Cell Sci. 110, 369 (1997); W. M. Michael, M. Choi, G. Dreyfuss, Cell 83, 415 (1995); W. M. Michael, P. S. Eder, G. Dreyfuss, EMBO J. 16, 3587 (1997).
    • (1997) J. Cell Sci. , vol.110 , pp. 369
    • Arenzana-Seisdedos, F.1
  • 24
    • 0028845313 scopus 로고
    • U. Fischer, J. Huber, W. C. Boelens, I. W. Mattaj, R. Luhrmann, ibid. 82, 475 (1995); W. Wen, J. L. Meinkoth, R. Y. Tsien, S. S. Taylor, ibid., p. 463; F. Arenzana-Seisdedos et al., J. Cell Sci. 110, 369 (1997); W. M. Michael, M. Choi, G. Dreyfuss, Cell 83, 415 (1995); W. M. Michael, P. S. Eder, G. Dreyfuss, EMBO J. 16, 3587 (1997).
    • (1995) Cell , vol.83 , pp. 415
    • Michael, W.M.1    Choi, M.2    Dreyfuss, G.3
  • 25
    • 0030978415 scopus 로고    scopus 로고
    • U. Fischer, J. Huber, W. C. Boelens, I. W. Mattaj, R. Luhrmann, ibid. 82, 475 (1995); W. Wen, J. L. Meinkoth, R. Y. Tsien, S. S. Taylor, ibid., p. 463; F. Arenzana-Seisdedos et al., J. Cell Sci. 110, 369 (1997); W. M. Michael, M. Choi, G. Dreyfuss, Cell 83, 415 (1995); W. M. Michael, P. S. Eder, G. Dreyfuss, EMBO J. 16, 3587 (1997).
    • (1997) EMBO J. , vol.16 , pp. 3587
    • Michael, W.M.1    Eder, P.S.2    Dreyfuss, G.3
  • 26
    • 1842263762 scopus 로고    scopus 로고
    • note
    • The complete coding sequence of human CRM1 was amplified by polymerase chain reaction from an HPBALL cell cDNA library and cloned into the Kpn I and Xba I sites of pcDNA3 plasmid (Invitrogen).
  • 27
    • 1842286220 scopus 로고    scopus 로고
    • note
    • 35S-cysteine (Amersham). Translation products were analyzed by SDS-PAGE and autoradiography. For immunoprecipitation experiments, CRM1 was cotranslated with either wild-type IκBα or LκBα-L234. Five microliters of each TNT reaction was incubated in 40 μl of phosphate-buffered saline (PBS) containing BSA (100 μg/ml) for 30 min at room temperature before being immunoprecipitated with 2.5 μg of either anti-MYC tag or anti-SV5 tag in the presence of 20 μl of protein G-Sepharose (Pharmacia). After being washed in PBS containing 0.1% NP-40, samples were treated with Laemmli sample buffer for 2 min at 95°C and analyzed by 7% SDS-PAGE and fluorography. We obtained NES or mutated NES affinity columns by coupling biotinylated BSA (Pierce) first to sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Pierce) and then to NES peptide (CIQQQLGQLTLENL) or mutated NES peptide (CIQQQAGQATAENA) (9). For each condition, 16 μg of biotinylated BSA coupled to the peptides was bound to 20 μl of streptavidin-agarose. After being washed in PBS, beads were incubated in 40 μl of PBS containing BSA (100 μg/ml), 3 μl of the TNT reaction, and peptides (2 mg/ml) for the competition experiments for 30 min at room temperature. Unbound fractions were collected and sedimented material was extensively washed in PBS before being treated with Laemmli sample buffer for 2 min at 95°C. Samples were analyzed by 7% SDS-PAGE and fluorography.
  • 28
    • 1842264731 scopus 로고    scopus 로고
    • note
    • Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; E, Glu; G, Gly; I, Ile; L, Leu; N, Asn; Q, Gln; and T, Thr.
  • 30
    • 0025014776 scopus 로고
    • M. Yoshida et al., Exp. Cell Res. 187, 150 (1990); K. Nishi et al., J. Biol. Chem. 269, 6320 (1994).
    • (1990) Exp. Cell Res. , vol.187 , pp. 150
    • Yoshida, M.1
  • 31
    • 0028318159 scopus 로고
    • M. Yoshida et al., Exp. Cell Res. 187, 150 (1990); K. Nishi et al., J. Biol. Chem. 269, 6320 (1994).
    • (1994) J. Biol. Chem. , vol.269 , pp. 6320
    • Nishi, K.1
  • 32
    • 1842320560 scopus 로고    scopus 로고
    • note
    • 6 cells/ml. Fifty microliters of DNA mix (210 mM NaC;, 10 μg of specific DNA, and 30 μg of carrier DNA) was added to 200 μl of cell suspension before electroporation (950 μF, 240 V, with Gene Pulser II; Bio-Rad). Cells were subsequently cultured for 18 hours before analysis. Forty percent of cells were transfected by this protocol.
  • 33
    • 0025083331 scopus 로고
    • 2, 1 mM EGTA, 1 mM dithiothreitol (DTT), and protease inhibitors (leupeptin, pepstatin, and aprotinin) for 5 min at 4°C. After being washed twice in the same buffer, cells were incubated for 30 min at 23°C in the different incubation conditions. A high-speed supernatant was prepared from Xenopus eggs resuspended in 20 mM Hepes (pH 7.5), 70 mM KCl, 1 mM DTT, and 250 mM sucrose and centrifuged at 13,000g and then at 190,000g. The addition of ATP corresponded to 1 mM ATP, 10 mM creatine phosphate, and creatine phosphokinase (4 U/ml).
    • (1990) J. Cell Biol. , vol.111 , pp. 807
    • Adam, S.A.1    Stern-Marr, R.2    Gerace, L.3
  • 34
    • 1842396921 scopus 로고    scopus 로고
    • note
    • For indirect immunofluorescence analysis, cells were fixed for 10 min with 2% paraformaldehyde and 0.1% glutaraldehyde and permeabilized with 0.1% Triton X-100 for 5 min. A monoclonal antibody (mAb) to MYC (9E10) was applied for 30 min followed by a 30-min incubation with fluorescein isothiocyanate-conjugated donkey anti-mouse immunoglobulin G (Jackson). Cover slips were mounted in Moviol (Hoechst, Frankfurt, Germany). Photographs corresponding to the different conditions were taken with the same setting parameters.
  • 35
    • 1842381355 scopus 로고    scopus 로고
    • note
    • Cells were treated first with 1 μg of deoxyribonuclease I before being lysed in Laemmli sample buffer containing 8 M urea. Proteins were resolved by 10% SDS-PAGE and transferred to nitrocellulose membrane. Membranes were incubated with mAb 9E10 and an mAb to hnRNP C (4F4), followed by an incubation with anti-mouse coupled to horseradish peroxidase, and finally developed with the chemiluminescence protein immunoblotting reagents (POD, Boehringer Mannheim, Germany). Quantitation of protein immunoblots was performed with the Bioprint acquisition system and Bioprofil program (Vilbert Lourmat).
  • 38
    • 0029098538 scopus 로고    scopus 로고
    • C. C. Fritz, M. L. Zapp, M. R. Green, Nature 376, 530 (1995); F. Stutz, M. Neville, M. Rosbash, Cell 82, 495 (1995); H. P. Bogerd, R. A. Fridell, S. Madore, B. C. Cullen, ibid., p. 485; F. Stutz, E. Izaurralde, I. W. Mattaj, M. Rosbach, Mol. Cell. Biol. 16, 7144 (1996).
    • (1995) Nature , vol.376 , pp. 530
    • Fritz, C.C.1    Zapp, M.L.2    Green, M.R.3
  • 39
    • 0029122732 scopus 로고
    • C. C. Fritz, M. L. Zapp, M. R. Green, Nature 376, 530 (1995); F. Stutz, M. Neville, M. Rosbash, Cell 82, 495 (1995); H. P. Bogerd, R. A. Fridell, S. Madore, B. C. Cullen, ibid., p. 485; F. Stutz, E. Izaurralde, I. W. Mattaj, M. Rosbach, Mol. Cell. Biol. 16, 7144 (1996).
    • (1995) Cell , vol.82 , pp. 495
    • Stutz, F.1    Neville, M.2    Rosbash, M.3
  • 40
    • 0029098538 scopus 로고    scopus 로고
    • C. C. Fritz, M. L. Zapp, M. R. Green, Nature 376, 530 (1995); F. Stutz, M. Neville, M. Rosbash, Cell 82, 495 (1995); H. P. Bogerd, R. A. Fridell, S. Madore, B. C. Cullen, ibid., p. 485; F. Stutz, E. Izaurralde, I. W. Mattaj, M. Rosbach, Mol. Cell. Biol. 16, 7144 (1996).
    • Cell , vol.485
    • Bogerd, H.P.1    Fridell, R.A.2    Madore, S.3    Cullen, B.C.4
  • 41
    • 0029923982 scopus 로고    scopus 로고
    • C. C. Fritz, M. L. Zapp, M. R. Green, Nature 376, 530 (1995); F. Stutz, M. Neville, M. Rosbash, Cell 82, 495 (1995); H. P. Bogerd, R. A. Fridell, S. Madore, B. C. Cullen, ibid., p. 485; F. Stutz, E. Izaurralde, I. W. Mattaj, M. Rosbach, Mol. Cell. Biol. 16, 7144 (1996).
    • (1996) Mol. Cell. Biol. , vol.16 , pp. 7144
    • Stutz, F.1    Izaurralde, E.2    Mattaj, I.W.3    Rosbach, M.4
  • 42
    • 85045586526 scopus 로고    scopus 로고
    • S. A. Richards, K. L. Carey, I. G. Macara, Science 276, 1842 (1997); T. Tachibana, M. Hieda, T. Sekimoto, Y. Yoneda, FEBS Lett. 397, 177 (1996).
    • (1997) Science , vol.276 , pp. 1842
    • Richards, S.A.1    Carey, K.L.2    Macara, I.G.3
  • 44
    • 1842315710 scopus 로고    scopus 로고
    • note
    • We thank Novartis and particularly B. Wolff for leptomycin B; G. Dreyfuss for the anti-hnRNP C; C. Maison for helpful and stimulating discussions; and D. Louvard, J. Salamero, and R. Golsteyn for critical reading of the manuscript. Supported by grants from Action Thematique et Incitative sur Programme et Equipes-CNRS, the Association de Recherche Contre le Cancer, and the European Communities Concerted Action (project: Rocio II).


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